• Design & Manufacturing
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• v.2 no.5
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• pp.5-10
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• 2008

Recently, ultra precision and light-weight micro products are needed in various industries. Injection molding products with metal insert material is often satisfied with light-weight and precision simultaneously. The researches on macro-size insert deformation have been performed but, a research on micro-size insert is meager. In this paper, the injection molding product with $300{\mu}m$ thin foil insert is designed and insert injection molding process is performed. Finally, the deformation of thin foil insert is analyzed according to insert feature and gate length.

• Korean journal of applied entomology
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• v.54 no.2
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• pp.91-97
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• 2015

The sweetpotato whitefly, Bemisia tabaci, is the major insect pest that transmitted over 100 plant viruses including tomato yellow leaf curl virus (TYLCV) of tomato plant as virus vector in the world. In this study, cDNA library of whitefly was constructed using Gateway system for selecting target gene in order to control of B. tabaci using virus-induced gene silencing (VIGS) vector with RNAi. First of all, when using oligo d(T) rimer, the calculated titer of cDNA library was confirmed with $1.4{\times}10^4$ clones and average insert sizes was confirmed with 1 kb. However, insert size was very big for construction of cDNA. Otherwise, when using attB-N25 random primer and sonication for 6 sec, the calculated titer of cDNA library was confirmed with $1.04{\times}10^5$ clones. But mostly insert band wasn't identified on the electrophoresis, because it seemed that insert size is too small (${\leq}100bp$), also the size of identified insert was somewhat big. Finally, when using oligo d(T) primer and sonication for 1 sec, cDNA insert of whitefly was appropriated for VIGS with 300-600 bp. However, cDNA sequence included a poly A and titer was very low to $5.2{\times}10^2$ clones. It was supposed that heat shock transformation was used instead of electro-transformation. It is considered that when constructing cDNA library for using VIGS vector, (1) random primer should be used for First strand cDNA synthesis in order to remove poly A and (2) sonication for 1 sec should be performed in order to get appropriated insert size and (3) electro-transformation should be performed in order to improve transformation efficiency.

• Journal of Mechanical Science and Technology
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• v.16 no.7
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• pp.1019-1028
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• 2002

The instability of two-phase loop thermosyphons was investigated experimentally and analytically. Three orifice type inserts were used to study the effect of change in the pressure drop in the flow channel of the TLT on the flow instability and temperature fluctuation. It is observed that a decrease in the size of the orifice insert from 3.7 mm (no insert) to 0.71 mm drastically reduced the fluctuation of the temperature, especially at the evaporator section of the TLT. With the orifice type insert of 0.71 mm for the TLT, the overall temperature fluctuation was almost completely eliminated, especially at higher power input to the TLT The analysis based on the Kelvin-Helmholtz instability theory seems to predict reasonable well the loop stability state of the TLT with experimentally determined constant factors.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.14 no.5
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• pp.408-414
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• 2002

The instability of two-phase loop thermosyphons (TLTs) was investigated experimentally. Three orifice type inserts were used to study the effect of change in the pressure drop in the flow channel of the TLT on the flow instability and temperature fluctuation. It is observed that a decrease in the size of the orifice insert from 3.7mm (no insert) to 0.71mm drastically reduced the fluctuation of the temperature, especially at the evaporator section of the TLT With the orifice type insert of 0.71 mm for the TLT, the overall temperature fluctuation was almost completely eliminated, especially at higher power input to the TLT.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2008.05a
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• pp.535-538
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• 2008

Insert-injection molding can inject two different materials or two colors in the same mold and process. If this injection process use, product has ability because the base part maintain strength and specified part can inject soft-material. It makes the cost down by single operation automatically for saving wages. In this paper, we designed double-injection mold for automobile remote control to inject secondary using this part as insert after inject external appearance of product. CAE analysis was progressed gate location and runner size as variable and analysis result is reflected in mold design process. As a result, it could solved badness that is generated at the conventional mold. Additionally, cost is downed by reducing loss of runner as well as could omit painting process because surface of finished product is improved through new mold.

• Design & Manufacturing
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• v.11 no.3
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• pp.29-34
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• 2017

Increased demand for parts with micro-pattern structure made of metals, ceramics, and composites in various fields such as medical ultrasonic sensors, CT collimators, and ultra-small actuator parts. Micro powder injection molding (PIM) is a technology for manufacturing micro size, high volume, complex, precision, net-shape components from either metal or ceramic powder. In the present study, a standard mold with a variable insert core capable of producing various micro patterns was investigated. An injection molding test was performed on a standard mold using a line type micro-pattern core having an aspect ratio of 2, a slenderness ratio of 70, a pattern size of $200{\mu}m$, and a pattern spacing of $150{\mu}m$. During the filling process, the deformation of the mold with large aspect ratio and slenderness ratio was analyzed by the experiment and the numerical simulation according to the position of the gate. We proposed a mold structure that minimizes mold deformation by gate modification and enables uniform pattern filling behavior.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.2
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• pp.565-570
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• 2011

There is always die roll in fine blanking parts which is able to have 100% clean shear surface. In this paper the change of die roll size was studied by fine blanking tryout in order to minimize die roll size. Various die inserts with different die chamfer were machined, fine blanking die was manufactured and tested. The die roll sizes of fine blanking samples were measured and the tendency of thickness directional die roll size was comprehended. This result will be used on the design of die chamfer in order to minimize thickness directional die roll size of fine blanking parts

• Journal of Korean Tunnelling and Underground Space Association
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• v.21 no.1
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• pp.49-59
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• 2019

In this study, the durability of conical pick cutter was compared and analyzed by pre- and post-test visual inspection, measurement of weight loss and wear volume through field test on two types of conical pick cutters applied to rotary drum cutter. In the visual inspection, it was found that only 9 inserts were lost in the slim type conical pick cutter. This result show that the thickness of the head cover surrounding a insert was important to maintain the insert during excavation. The weight loss and wear volume of the heavy type conical pick cutter were less than half that of the slim type. From these results, it can be confirmed that heavy type is more useful than slim type in hard rock. It should be noted that, when determining the wear loss of the conical pick cutter, the mutual comparison of the weight measurement and the wear volume measurement results may be different due to the unit weight of the material and the spalling caused by excavation.

• Genomics & Informatics
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• v.4 no.2
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• pp.80-86
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• 2006

It is clear that the construction of large insert DNA libraries is important for map-based gene cloning, the assembly of physical maps, and simple screening for specific genomic sequences. The bacterial artificial chromosome (BAC) system is likely to be an important tool for map-based cloning of genes since BAC libraries can be constructed simply and analyzed more efficiently than yeast artificial chromosome (YAC) libraries. BACs have significantly expanded the size of fragments from eukaryotic genomes that can be cloned in Escherichia coli as plasmid molecules. To facilitate the isolation of molecular-biologically important genes in Ashbya gossypii, we constructed Ashbya chromosome-specific BAC libraries using pBeloBAC11 and pBACwich vectors with an average insert size of 100 kb, which is equivalent to 19.8X genomic coverage. pBACwich was developed to streamline map-based cloning by providing a tool to integrate large DNA fragments into specific sites in chromosomes. These chromosome-specific libraries have provided a useful tool for the further characterization of the Ashbya genome including positional cloning and genome sequencing.

• Design & Manufacturing
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• v.14 no.1
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• pp.15-21
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• 2020

Smart systems in different application areas such as automotive, medical and consumer electronics require a novel manufacturing method of electronic, optical and mechanical functions into products. Traditional methods including mechanical assembly, bonding of plastic and electronic circuit cause the problems in large size of products and complicated manufacturing processes. In this study, thermoforming and film insert molding were applied to fabricate a dome shaped plastic part embedded with electronic circuits. The deformation of patterns printed on PET film was predicted by thermoforming simulation using T-SIM, and the results were compared with those by experiment. In order to decrease spring-back after thermoforming, the Taguchi method of design of experiment was used. Through ANOVA analysis, it was found that mold temperature was the most dominant parameter for spring-back. By using flow analysis, gate design was performed to decrease injection pressure. During film insert molding, the wash-out of ink printed on film occurred for Polycarbonate. When the resin was changed to PMMA, the wash-out disappeared due to low melt temperature.