• Title/Summary/Keyword: Insect resistance

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Control Indian meal moth Plodia interpunctella by gas treatment

  • Han, Gyung Deok;Kwon, Hyeok;Jin, Hyun Jung;Kum, Ho Jung;Kim, Bo Hwan;Kim, Wook
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.45-45
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    • 2017
  • The Indian meal moth, Plodia interpunctella, is one of the most important pests of stored food in the food processing industry worldwide. To control the Indian meal moth, methyl bromide, phosphine, high carbon dioxide, sulfuryl fluoride and plant essential oil fumigation have been considered. However, these treatments have disadvantages. For example, depleting the ozone layer, showing resistance in insect, low control efficacy or need high cost for treatment. Chlorine dioxide ($ClO_2$) is strong disinfectant and insecticide. The gas caused a malfunction in enzymes. The oxidative stress induced by $ClO_2$ gas treatment damaged to a physiological system and all life stages of P. interpunctella. The gaseous $ClO_2$ is a convincing alternative to methyl bromide for controlling P. interpunctella. The gaseous $ClO_2$ was generated by a chlorine dioxide generator (PurgoFarm Co., Ltd., Hwasung, Korea). It generated highly pure $ClO_2$ gas and the gas blown out through a vent into a test chamber. Gas entry to the chamber was automatically controlled and monitored by a PortaSene II gas leak detector (Analytical Technology, Collegeville, PA, USA). The properly prepared eggs, larvae, pupae, and adults of P. interpunctella were used in this experiment. Data were analyzed using SAS 9.4. Percentage data were statistically analyzed after arcsine-root transformation. Analysis of variance was performed using general linear model, and means were separated by the least significant difference test at P < 0.05. Fumigation is an effective management technique for controlling all stages of P. interpunctella. We found that $ClO_2$ gas treatment directly effects on egg, larvae, pupae and adults of P. interpunctella. The gas treatment with proper concentration for over a day achieved 100 % mortality in all stages of P. interpunctella and short time treatment or low concentration gas treatment results showed that the egg hatchability, pupation rate, and adult emergency rate were lowered compare with untreated control. Also, abnormal pupae or adult rate were increased. Gaseous $ClO_2$ treatment induced insecticidal reactive oxygen species (ROS), and it resulted in fatal oxidative stress in P. interpunctella. Taken together, these results showed that exposure proper concentration and time of the gas control all stages of P. interpunctella by inducing fatal oxidative stress. Further studies will be required to apply the gas treatment under real-world condition and to understanding physiological reaction in P. interpunctella caused by oxidative stress.

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The Survey of Actual Using Conditions of Farm-Made Liquid Fertilizers for Cultivating Environment-friendly Agricultural Products (친환경 농산물 재배를 위한 농가 자가제조 액비 사용실태)

  • An, Nan-Hee;Jo, Young-Sang;Jo, Jeong-Rae;Kim, Yong-Ki;Lee, Yeon;Jee, Hyeong-Jin;Lee, Sang-Min;Park, Kwang-Lai;Lee, Byung-Mo
    • Korean Journal of Organic Agriculture
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    • v.20 no.3
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    • pp.345-356
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    • 2012
  • We conducted a survey of actual using conditions of farm-made liquid fertilizers by investigating their formulation types, materials, making processes, using methods and various beneficial effects on 29 farms certified by National Agricultural Products Quality Management Service to produce environment-friendly agricultural products in 2009. Most of the materials used to make liquid fertilizers are those that can be easily obtained around the farms. Molasses or black sugar are added as an energy source of microorganism. And leaf mold, bacterial cultures supplied by agricultural extension centers of local governments, and cultures of native microorganisms were used as microbial sources for fermenting effective microorganisms. Types of the farm-made liquid fertilizers were fermented liquid fertilizers, fermented plant juices, amino acid liquid fertilizers, calcium-liquid fertilizers, and phosphoric acid liquid fertilizers. Effects of liquid fertilizers used by the farms were found to promote plant growth by supplying nutrition, to accelerate blooming and flower bud formation, to enhance the quality of agricultural products such as increase of sugar contents and improvement of storing conditions, to induce resistance against diseases and insect pests, and to cause endurance to high temperature stress. Chemical properties of the liquid fertilizers collected were analyzed. As a result, pH and EC range showed differences according to kinds of the liquid fertilizers. Amount of macro-nutrients, such as nitrogen and phosphoric acid, in most of the collected liquid fertilizers, was found to be low. Even though the liquid fertilizers were made from same materials, their contents was found to be different depending on the making process.

Effect of Different Drained Conditions on Growth, Forage Production and Quality of Silage Corn at Paddy Field (논에서 배수조건에 따른 사일리지용 옥수수 품종의 생육특성, 생산성 및 품질 비교)

  • Ji, Hee-Chung;Kim, Won-Ho;Kim, Ki-Yong;Lee, Sang-Hoon;Yoon, Sei-Hyung;Lim, Young-Chul
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.29 no.4
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    • pp.329-336
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    • 2009
  • This experiment was carried out to know adaptability and forage production and quality of corn hybrid for silage at paddy field from 2007 to 2008 at Chungnam province. Growth, forage production and quality of silage corn showed more well drained condition than poorly drained condition at paddy field. Among growth characteristics, 'Kwangpyongok' and 'DK697' hybrids were somewhat strong for waterlogging, then and good at stay green, lodging, disease and insect resistance. Fresh yield of 'DK697' hybrid at poorly drained paddy field was the highest as 32,610 kg per ha among corn hybrids. The dry yield of 'P32P75' hybrid at poorly drained paddy field was the highest as 14,910 kg per ha The result of this study showed that 'P32P75', 'DK697', 'Kangdaok' and 'Kwangpyongok' hybrids had good growth characters and forage productivity at poorly drained paddy field and dry matter yield at poorly drained paddy field was 65.6% level compared with well drained paddy field.

Selection of Pasture Species at Paddy Field in Southern Region of Korea (남부지역 논 재배에 적합한 목초류 초종 선발)

  • Ji, Hee-Chung;Kwon, Oh-Doo;Kim, Won-Hoo;Lim, Young-Chul;Cho, Jung-Ho;Lee, Ki-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.2
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    • pp.113-118
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    • 2011
  • This experiment was carried out to select the adaptability, forage production and quality of pasture species at paddy field from 2007 to 2009 at Jeonnam province. Among pasture species, Italian ryegrass was somewhat good for waterlogging, production, and disease and insect resistance. Fresh and dry matter yields of Italian ryegrass was the highest as 67,368 kg and 17,677 kg per ha among six species. The dry yield of Tall fescue mixture (Orchardgrass, Tall fescue, Perennial ryegrass, Kentuck bluegrass, White clover) and Tall fescue were also high as 15,589 kg and 14,693 kg per ha, respectively. Crude protein of Tall fescue and Tall fescue mixture were high as 10.4% and 13.5%, respectively. The result of this study showed that Italian ryegrass, Tall fescue mixture and Tall fescue had good growth characteristics and forage productivity at paddy field in southern region of Korea.

Suppression of Powdery Mildew Development in Oriental Melon by Silicate Fertilizer (규산질 비료의 참외 흰가루병 발생 억제 효과)

  • Ryu, Na-Hyun;Choi, Mi-Young;Ryu, Youn-Ju;Cho, Hyun-Jong;Lee, Yong-Se;Lee, Young-Deuk;Chung, Jong-Bae
    • Korean Journal of Environmental Agriculture
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    • v.22 no.4
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    • pp.255-260
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    • 2003
  • Silicon is known to accumulate in plants and results in greater resistance to diseases and insect pests. In this study, we investigated the effect of silicate fertilizer applied in soil on the development of powdery mildew of oriental melon. Oriental melon seedlings of four-leaf stage were transplanted and grown in a plastic film house. Silicate fertilizer was applied to maintain soil available $SiO_2$ level of 200 mg/kg one week before transplanting. Fungicide triflumizol was sprayed three times; one, two, and three weeks after transplanting. Sphaerotheca fuliginea was inoculated 2 weeks after transplanting. The number of infected leaf and the number of fungal colony in leaves were measured one, two, and three weeks after the inoculation. Three weeks after the fungal inoculation, in the treatment of fungicide triflumizol. infected leaf numbers and number of colony per leaf were reduced by 10 and 58%, respectively. In the silicate fertilizer treatment, infected leaf numbers and numbers of colony per infected leaf were suppressed only by 6 and 16%, respectively, and the efficacy was lower than that of the fungicide triflumizol. The combined treatment of silicate fertilizer and the fungicide suppressed powdery mildew more effectively, and infected leaf numbers and numbers of colony per leaf were reduced by 31 and 80%, respectively. These results indicate that although silicate fertilizer itself is not much effective in the suppression of powdery mildew, it can significantly enhance the efficacy of the fungicide.

A New Early Maturity, High Grain Quality and Cold Tolerance Rice Cultivar "Boseog" (벼 조생 고품질 내냉성 "보석")

  • Nam, Jeong Kwon;Kim, Ki Young;Choung, Jin Il;Shin, Mun Sik;Kim, Bo Kyeong;Ha, Ki Yong;Ko, Jong Cheol;Kang, Hyeon Jung;Shin, Woon Chul;Mo, Young Jun;Ko, Jae Kwon
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.564-567
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    • 2009
  • "Boseog" is a new japonica rice cultivar developed from the three way cross of Kinuhikari//HR19621AC6/Sobibyeo and registered by the rice breeding team of Department of Rice and Winter Cereal Crop, NICS, RDA in 2008. This cultivar has about 110 days of growth duration from transplanting to harvesting in northern plain, northern and southern mid-mountainous area of Korea. This cultivar is about 72 cm in culm length and tolerant to lodging. In the reaction to biotic and abiotic stresses, it shows resistance to leaf blast, but susceptibility to bacterial blight, virus diseases and insect pests. The milled rice of "Boseog" exhibits translucent, relatively clear non-glutinous endosperm and midium short grain. It has similar amylose content(18.0%), lower protein content(6.3%), and similar palatability of cooked rice compared to Odaebyeo. The milled rice yield of this variety is about 5.03 MT/ha in local adaptability test. "Boseog" would be adaptable for ordinary transplanting in northern plain, mid-mountainous and southern mountainous area of Korea.

Effect of Brown Planthopper Resistance Gene, Bph18 to Yield Components in Rice (벼멸구 저항성 Bph18 유전자가 벼의 수량구성요소에 미치는 영향)

  • Shin, Mun-Sik;Kim, Woo-Jae;Shin, Woon-Chul;Park, Hyun-Su;Seo, Chun-Sun;Choi, In-Bae;Ha, Ki-Yong;Kang, Hyun-Jung;Ko, Jae-Kwon
    • Korean Journal of Breeding Science
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    • v.43 no.1
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    • pp.56-61
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    • 2011
  • Brown planthopper(BPH) is one of the most destructive insect pests of rice. The use of genetically resistant cultivars has proven to be a more economical, efficient and environment friendly means to combat this pest. This study was carried out to investigate the relationship between BPH resistant gene, Bph18 and yield components of rice using DH(doubled haploid) lines derived from 'Saenuri'/SR30071-3-7-23-6-1-1-1. SR30071-3-7-23-6-1-1-1 line has Bph18 gene derived from wild species, Oryza australiensis. BPH resistant gene, Bph18 shortened heading days, enlarged culm length and panicle length and reduced ratio of ripened grains of rice. The results indicate that backcrossing breeding is necessary to develop elite cultivars carrying Bph18 gene.

Biological Control of Thrips Using a Self-produced Predatory Mite Stratiolaelaps scimitus (Acari: Laelapidae) in the Greenhouse Chrysanthemum (시설재배지 국화에서 자가생산한 뿌리이리응애 (응애아강: 가시진드기과)를 활용한 총채벌레의 생물적 방제)

  • Jung, Duck-Oung;Hwang, Hwal-Su;Kim, San-Young;Lee, Kyeong-Yeoll
    • Korean journal of applied entomology
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    • v.58 no.3
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    • pp.233-238
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    • 2019
  • For greenhouse crops, thrips is one of the major insect pests, but its control is difficult owing to short generation time, rapid escaping behavior, and development of pesticide resistance. Stratiolaelaps scimitus (Womersley) is a soil-dwelling predatory mite attacking various soil invertebrate species, including thrips. Using the method by growers' self-production, we mass-reared S. scimitus colony and investigated thrips control in the greenhouse chrysanthemum. The initial density of thrips was six individuals/flower. The treatment with one S. scimitus box in the greenhouse was estimated to be $1,000individuals/m^2$. From August to September 2018, the greenhouse was released with a total of 10 boxes of S. scimitus. The density of thrips in the untreated and the treated cultivar was $53.7{\pm}7.0$ and $13.5{\pm}1.7$ on the late September, respectively, which indicated a reduction of 74.9% in the treated cultivar. Our results suggest that S. scimitus was highly effective for the control of thrips in the greenhouse chrysanthemum although temperature is very high during the summer season.

Selection of Entomopathogenic Fungus Isaria javanica FT333 for Dual Control of Thrips and Anthracnose (총채벌레 및 고추탄저병의 동시 방제를 위한 곤충병원성 곰팡이 Isaria javanica FT333 선발)

  • Lee, Moran;Jeong, Hyeju;Kim, Jaeyoon;Kim, Dayeon;Ahn, Seung Ho;Lee, SangYeob;Han, Ji Hee
    • The Korean Journal of Mycology
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    • v.46 no.4
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    • pp.479-490
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    • 2018
  • Red pepper is seriously damaged by thrips (Thrips palmi) and anthracnose caused by Colletotrichum acutatum throughout its development. Because of biotic constraints, producers often depend on chemicals that are expensive and have adverse effects on the environment, operator, and beneficial insects. In addition, resistance is developed because of the repeated use of chemicals. In recent decades, the use of microorganisms in crop protection has become a credible alternative because it is eco-friendly. In this study, we aimed to select isolates with insecticidal and fungicidal activities against the pathogens that cause anthracnose and thrips. We treated T. palmi adults and juveniles with 13 strains of entomopathogenic fungi (isolated from the soil by using the insect-bait method), and 6 strains showed excellent insecticidal activity (70-100%) 5 days after the treatment. The selected isolates were cultured with C. acutatum to screen for the strain with excellent anti-fungal activities, among which an isolate FT333 showed more than 95% control efficacy against C. acutatum in vitro. The isolate was identified as Isaria javanica through its morphological characteristics and phylogenetic analysis of the ITS and ${\beta}-tubulin$ nucleotide sequences. The Isaria javanica FT333 isolate could be used effectively for dual bio-control of thrips and anthracnose during red pepper cultivation.

Development of Sequential Sampling Plan of Bemisia tabaci in Greenhouse Tomatoes (토마토 온실내 담배가루이의 축차표본조사법 개발)

  • SoEun Eom;Taechul Park;Kimoon Son;Jiwon Jeong;Jung-Joon Park
    • Korean journal of applied entomology
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    • v.62 no.4
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    • pp.299-305
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    • 2023
  • Bemisia tabaci is one of polyphagous insect pests that transmits Tomato Yellow Leaf Curl Virus (TYLCV) and Cassava Brown Streak Disease (CBSD). Insecticides are primarily applied to control B. tabaci, but it has limits due to the development of resistance. As a result, a fixed precision sampling plan was developed for its integrated pest management (IPM). The tomato plants were divided into top (more than 130cm from the ground), middle (70 cm to 100 cm above the ground), and bottom (50 cm or less above the ground) strata, before visual sampling of the larvae of B. tabaci. The spatial distribution analysis was conducted using Taylor's power law coefficients with pooled data of top, middle, bottom strata. Fixed precision sampling plan and control decision-making were developed with precision levels and action threshold recommended from published scientific papers. To assess the validation of the developed sampling plans, independent data not used in the analysis were evaluated using the Resampling Validation for Sampling Plan (RVSP) program.