• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2004.05a
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• pp.83-83
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• 2004

• Korean Journal of Ecology and Environment
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• v.53 no.1
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• pp.73-79
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• 2020

Metarhizium anisopliae is one of the entomopathogenic fungi infecting Protaetia brevitarsis seulensis. Environmental conditions strongly affect the virulence of entomopathogenic fungus. To test the susceptibility of P. brevitarsis seulensis larvae to M. anisopliae, we determined level mortality rates under different rearing conditions: temperatures (20℃, 25℃, 30℃), relative humidities (RH; RH 40%, 50%, 60%, 70%), and water content in sawdust (40%, 50%, 60%). The mortality rate of larvae treated with 5×10⁶ conidia mL^-1 M. anisopliae was not significantly affected by rearing temperature, but was affected by low RH (40%) and low water content in sawdust (40%), both of which resulted in high mortality rates. Mortality rated among M. anisopliae-treated 1^st, 2^nd, and 3^rd instar larvae were highest in 1^st instar larvae.

• International Journal of Industrial Entomology and Biomaterials
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• v.32 no.1
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• pp.41-48
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• 2016

Mealworms, Tenebrio molitor (Coleoptera: Tenebrionidae), are widely used as food sourcefor animal rearing as well as human diet. Conventionally, mealworms raised on wheat bran. In this study, we investigated the effects of brewer's spent grain (BSG) on the growth of mealworm larvae to reduce the rearing cost. We prepared five different diets with various BSG content, 0, 10, 30, 50, and 70% of heated air dried BSG with wheat bran. We compared survivorship, larval weight, developmental duration, pupation rate, and pupal weight of five different groups of T. molitor raised on these five diets. The larval survivorships were almost similar with the control group except a group with 70% of BSG. For the larval weight, groups with 30% and 50% of BSG gained more weight than that with control treatment, 0% of BSG. For the developmental period, the groups with 30% of BSG took less time compared to the control group. In all treatment groups, more than 90% of pupation rate were observed. Especially, the group with 30% of BSG showed highest pupation rate among the five groups. Wheat bran with 30~50% of BSG was the optimal diet for successive insect rearing among the five diet treatments. Based on this study, we concluded that adding BSG to wheat bran helps to improve the quality of T. molitor and to reduce the rearing period.

• Korean journal of applied entomology
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• v.31 no.3
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• pp.241-246
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• 1992

Since 1975, the studies on in vitro rearing Trichogramma spp., Anastatus japonicus Ashmead, Telenomus dendrolimusi Chu, Dibrachys cavus Walker, Habrobracon hebetor(Say), Bracon greeni Ashmead have been conducted successfully in China. The simulated “host-eggs" are made of polyethylene or polypropylene semispherical capsules, containing artificial diets, in which insectan pupal hemolymph is the main component. Manufacture of simulated “host larvae" are made of small rectangular parafilm of cotton-paper bags, containing artificial diets with insectan pupal hemolymph as the main component. Mass production of in vitro reared Trichogramma spp. and Anastatus japonicus and its utilization in the fields showed good effectiveness in controlling cotton bollworm, pine caterpillars, sugarcane borers and litchi stink bug.

• International Journal of Industrial Entomology and Biomaterials
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• v.29 no.2
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• pp.198-206
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• 2014

The olive fly Bactrocera oleae (Rossi) is the key pest for olive cultivation worldwide. Substantial effort has been invested in the development of the sterile insect technique (SIT) to control this pest. One of the limitations to develop SIT technology for olive fruit fly is the low ability of wild females to lay eggs in other medium than olive fruits, and their slow adaptation to oviposition in artificial substrates. In the present study, fruit grapes were used as an alternative egg collection medium to harvest eggs and young larvae from freshly colonized wild strains originating from France, Italy, Spain and Croatia. The larvae were allowed to develop into the fruits until the second instar, before they were extracted out and further reared on a standard artificial diet. Furthermore, F1 to F4 female flies were alternatively offered wax bottles to oviposit. Finally, the performance of hybrid strains created from crosses between wild and long colonised flies was assessed. The results showed that females of all 4 wild strains readily oviposited eggs in grapes and from the F2 generation onward, females from all strains were adapted to laying eggs in wax bottles. No difference was observed in eggs and pupae production among all strains tested. The findings are discussed for their implications on SIT application against olive fruit fly.

• Korean Journal of Ecology and Environment
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• v.53 no.4
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• pp.374-379
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• 2020

The white-spotted flower chafer (Protaetia brevitarsis) larva is one of the insects widely-used for edible and medicinal purposes in Eastern Asia. As a result of increasing demand for P. brevitarsis, massrearing systems in domestic farms have become necessary. However, the mass-rearing of larvae under confined rearing conditions could provide conditions unsuitable for preventing entomopathogenic diseases. Metarhizium anisopliae is the strongest fatal entomopathogenic fungus against P. brevitarsis. For inhibition of M. anisopliae, we used a Rhus verniciflua Stokes extract that has antifungal components. We investigated the inhibitory effect of the R. verniciflua extract at 1%, 5%, and 10% concentrations. The results showed that a 1% R. verniciflua extract added to sawdust produced a significantly low P. brevitarsis mortality rate. Moreover, extract-treated groups were heavier and had a shorter larval period than those of the untreated group. Consequently, we suggest that using an R. verniciflua extract can reduce the P. brevitarsis fatality rate from entomopathogenic fungi (e.g. M. anisopliae), resulting in more effective mass-rearing systems for P. brevitarsis.

• International Journal of Industrial Entomology and Biomaterials
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• v.28 no.1
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• pp.1-4
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• 2014

The effect of an artificial diet on developmental rate, a life history parameter, was examined for the swallowtail butterfly Papilio xuthus. Artificial insect diets are an essential component of many insect rearing systems that produce insects for research purposes. Complex agar-gelled diets are generally prepared in large batches and used shortly after preparation because the degradation of perishable diet ingredients, such as vitamins and fatty acids, can adversely affect insect quality (Brewer 1984). However, the timing of diet preparation may be inconvenient, and large batches wasteful, if the unused excess is discarded. The percentage of pupation varied considerably, with no significant differences among diets, on which a maximum pupation percentage of 83% was observed. Pellet-type diets were investigated with the aim of developing a more easily prepared diet. The extrusion of the artificial diet under high temperature and pressure may induce desirable chemical and physical changes in the extruded product. The purpose of the present study was to develop an artificial diet for rearing P. xuthus.

• Korean journal of applied entomology
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• v.55 no.4
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• pp.421-429
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• 2016

Tenebrio molitor larvae contain large amounts of proteins, lipids and other functional materials, enabling this insect to be used as an edible food source in animal feeds and for industrialization. Although many efforts have been made to set up mass rearing systems, few studies have been conducted to establish optimal rearing conditions for the production of high quality T. molitor larvae. Herein we investigated 1) the effects of additional diets on the survival and fecundity of the insect, 2) the relationship between oviposition period and the uniformity of larval size, 3) the effects of rearing density and temperature on insect development, and 4) the storage stability of eggs and pupae at low temperatures given possible temporary production discontinuation. The addition of carrot and zucchini to the traditional wheat bran diet significantly increased the survival and fecundity rate of adult T. molitor. Of the three different oviposition sampling periods (3, 7, and 14 days) used to investigate the uniformity of the hatched larvae in each treatment, the period of 3 and 7 days provided higher uniformity than the 14 days oviposition period. Larval development was faster at $30^{\circ}C$ than at 20, 25, and $35^{\circ}C$. Interestingly, oviposition rates were highest at $20^{\circ}C$ but showed much slower larval development and lower uniformity at $30^{\circ}C$. Regarding the effect of larval rearing densities (1, 10, 20, 30, 40, and 50 larvae per 90 mm diam. dish), larval weight was significantly reduced at higher rearing densities, but larval longevity and length were not influenced by rearing density. The 30 larvae/dish is suggested to be a reasonable density to be applied to mass production systems. When kept at $4^{\circ}C$, T. molitor eggs showed a significant reduction in hatching rate; however, when stored under the same conditions, pupae emergence rates remained high until 10 weeks, suggesting that storage at low temperatures is more suitable for the pupal stage than the egg stage. Our findings suggest that an increase in T. molitor adult survival and fecundity rates and a uniformity of hatched larval development can be achieved with the following recommendations: a combination diet (including wheat bran), a 7-day oviposition period; a larvae-rearing temperature of $30^{\circ}C$, a rearing density of 30 larvae/dish, and the storage of pupal stages at low temperatures in the case of rearing discontinuation. This study serves as a strong foundation for the successful mass production of high quality T. molitor larvae.

• International Journal of Industrial Entomology and Biomaterials
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• v.29 no.2
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• pp.193-197
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• 2014

To investigate the degree of individuals' likability of insects, a survey was conducted with students and adults living in Korea. Overall, for insect-related questions, positive responses were higher than negative ones. Among the positive responses, the personal rearing of insects scored lower than others. The unconditional dislike of insects scored highest in the negative responses. A significant differences and correlations were found between males and females, students and adults, people experienced in insect-related events and those who were not. In particular, previous experience in insect-related events significantly influenced insect likability. Subjects preferred coleopteran and lepidopteran insects to others. However, this preference of insects differed by gender: Male participants preferred coleopteran insects to lepidopteran insects, while female participants preferred lepidopteran insects to coleopteran ones. No significant differences in the preference of insects were found by participants' job, residence, or experience in insect-related events. These findings suggest that gender is the most important factor to consider in selecting insects to be used as therapeutic tools in mental health care.

• International Journal of Industrial Entomology and Biomaterials
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• v.26 no.1
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• pp.61-66
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• 2013

Baculum elongatum has several peculiarities such as parthenogenesis, unique external features, changing body color, and dropping oviposition, which makes it a potential economically useful insect. Oviposition was performed by females without fertilization by the sperm of males. The oviposition period was $42.2{\pm}22.7$ days and the number of eggs per female was $109.5{\pm}70.5$ eggs. The hatch rate was 73.3% at $25^{\circ}C$ and 66.7% after low temperature treatment ($8^{\circ}C$ for 60 days). In nature, B. elongatum overwinters as an egg, but it can be assumed from the results that cold temperatures were not required for hatching. The hatch rate was 98.2% in the treatment using floral foam, fermented sawdust, and leaves. The developmental period was $100.9{\pm}4.2$ days for eggs, $55.3{\pm}4.6$ days for nymphs, and $49.7{\pm}16.0$ days for adults. The length of the eggs was $0.33{\pm}0.0$ cm and the lengths of the nymphs were clearly distinguishable according to the instar stage. Clover(Trifolium repens) was an excellent diet as it was similar to the host plant and could be used as an alternative diet. The rate of reaching adulthood for the insects was 66.7% on an artificial diet containing 25% acacia leaves. For sustainable mass rearing of nymphs or adults of B. elongatum, a natural diet could be used such as acacia (Robinia pseudoacacia), white oak (Quercus aliena), chestnut (Castanea crenata var. dulcis), and bush clover (Lespedeza bicolor), or an alternative diet such as clover or artificial diet.