• 제목/요약/키워드: Inner Culture Contents

검색결과 39건 처리시간 0.02초

Concentrations of Calcium-binding Protein and Bone Gla-protein in Culture Medium and CaBP mRNA Expression in Osteoblasts of Broiler Chickens

  • Guo, Xiaoyu;Yan, Sumei;Shi, Binlin;Feng, Yongmiao
    • Asian-Australasian Journal of Animal Sciences
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    • 제24권2호
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    • pp.239-245
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    • 2011
  • This study was conducted to determine the effects of excess vitamin A on alkaline phosphatase (ALP) activity, contents of calcium-binding protein (CaBP), bone gla-protein (BGP) in culture medium and CaBP mRNA expression in chicken osteoblasts in vitro. Osteoblastic cells in the tibia from 1-day-old Arbor Acre broiler chickens were isolated using enzyme digestion. The subconfluenced cells were divided into eight treatments with six replicates in each treatment and cultured in a medium containing either vehicle or different levels of vitamin A (0, 0.2, 0.6, 1.0, 2.0, 5.0, 10.0 and $20.0\;{\mu}g$/ml), and the control received an equivalent volume of ethanol. The incubation lasted 48 h. The results showed that vitamin A down-regulated ALP activity in the culture medium as well as CaBP mRNA expression of osteoblasts in a linear dose-dependent manner (p = 0.124 and p<0.10, respectively), and suppressed the contents of BGP and CaBP in the culture medium in a quadratic dose-dependent manner (p<0.05 and p<0.10, respectively) with increasing addition of vitamin A. The addition of 0-$0.2\;{\mu}g$/ml vitamin A to the culture medium increased ALP activity, BGP and CaBP contents as well as CaBP mRNA expression compared with other groups, but positive effects of vitamin A tended to be suppressed when vitamin A was increased to $1.0\;{\mu}g$/ml, and adverse effects occurred when vitamin A was increased to 10.0-$20.0\;{\mu}g$/ml. These results implied that there was a threshold level of vitamin A inclusion beyond which inhibitory effects occurred, and the mechanism by which overdose of vitamin A reduced bone growth in chickens was probably reduced osteoblastic cell activity, and inhibited expression of CaBP mRNA and CaBP secretion.

한국여성의 가치관 변화에 관한 연구 -화장품과 의류상품 광고를 중심으로- (A Study on the Value Changes for the Korean Women in 1977~1998 -A Content Analysis of Print Ads-)

  • 전양진
    • 한국의류학회지
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    • 제25권4호
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    • pp.773-782
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    • 2001
  • This study was to investigate the value changes in Korean women by analyzing the ads of womens magazines. The contents of ads were classified into two types of values: general and consumption values. The general values, composed of inner-directedness and outer-directrdness, might predict consumer behavior in general. The consumption values, utilitarians and hedonic, were expected to affect the consumer purchasing attitude to a specific product. Factors affecting the cultural values were per capita income and womens social status. Total 2969 illustrated ads with verbal theme from 32 Korean womens magazines were used. The content analysis, chi-square test, logistic regression were done for the analysis. The results showed that inner-directedness was dominant general value in Korean womens culture and increased over time. Younger consumers were more inner-directed than older ones were, and inner-directed values increased with income growth. For the consumption values, utilitarian values were higher than hedonic ones and went up over time. For the product types, utilitarian ads were frequent in cosmetics while hedonic ads were high for apparels. Those results implied that Korean womens culture became more inner-directed with increased income. Also consumption values were likely to differ between product groups. Per capita income was shown to increase inner-directedness while womens social status was to increase outer-directedness.

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광복전후(光復前後) 공영주택(公營住宅)의 평면비교(平面比較)와 변화특성(變化特性)에 관한 연구(硏究) (A Study on the Comparison and Characteristics of Public Standard Housing Unit Plan before and after the Independence from the Japan Occupation)

  • 유재우
    • 건축역사연구
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    • 제11권2호
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    • pp.23-35
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    • 2002
  • Even though there have been greate changes in the types and contents of the housing plan in Korea since the Modem age, it seems that the identity of traditional dwelling culture is still remains within it. The purpose of this study is to examine the characteristics and features in the development of the 'Public Standard' Housing Unit Plan by comparing the differences and similarities in unit plans in housings built on Japanese occupation period and those built after the period. From the study, we can conclude as follows: 1. The public standard housing unit plans on both periods have shown the common features in the composition of their physical forms in the way that the front-row area still remains the traditional housing culture and the rear-row area reflects the desires of the modem lives. The structure of the dual-row unit plan appeared in the public standard housing plan was caused by the cultural acculturation in Japanese occupation period. 2. The public standard housing unit plans, however, show the differences in the contents and meaning in units plans with the them of the Japanese housing because of the cultural differences. These differences took placed autonomously through the change of housing plans in the modern time, and have the common features such that 1) the changes in the Maru-centered plan type differ in composition from the inner-corridor plan type used Japanese housing, and 2) the composition of the open-inner spaces in the double-row housing plan reflects the single-row outer open space In the traditional housing. All of these cultural progresses in the public standard housing unit plans was took place as the result of the inner-oriented and notional circulations derived from the modernity. However, the transformed unit plans still contains the identity derived from the variation of the traditional Korean building elements such as Maru and Ondol. In other words, those are the Maru-centered and composed housing plans with juxtaposition of the inner block and the outer block. As the conclusion, though the public standard dwelling unit plan has the similarity in the physical form, it is different in the cultural Identity and meaning from the public standard plans before the independence from the Japan occupation.

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농가맛집의 활성화를 위한 아이덴티티 디자인 개발과 적용 - 전북 김제 황금보리 밥상개발을 예로 - (The Development and Application of Identity Design to Facilitate Farm Restaurants - Developing menu of barley dishes for Hwanggeum Bori (golden barley) in Gimje, North Jeolla Province)

  • 장혜진;김수인
    • 디지털산업정보학회논문지
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    • 제9권4호
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    • pp.143-153
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    • 2013
  • The rural tourism in Korea is expanding in scope including experience tours and integrating with dining industry these days. The Rural Development Administration in Korea has been supporting the farm restaurant project across the nation as part of its efforts to utilize local or rural food recipes as tourism resource for seven years. Over the years, however, case studies are rarely done or reported regarding physical environment of farm restaurants. This study offers an opportunity for farm restaurateurs and policy makers related to recognize the importance of developing brand identity and inner culture contents for the rural community by presenting a case of integrated design marketing. In the study, definitions of the "authentic food" in Korea and other countries are compared before farm restaurants at home and abroad are investigated. It also addresses the foundation on the significance of physical environment and design to build a brand identity of authentic food. The case presented is naming, designing and developing logos and design applicable to various product packages for "Hwanggeum Bori Aechan", which is the barley (main crop in Gimje)- dish development project for Gimje, North Jeolla Province in 2012.

운봉 향교 정밀 모니터링을 통한 목재 기둥 부재의 함수율 분포 특성 (Study on Moisture Contents Distribution of Wooden Post Member by Precise Monitoring in Un-Bong Confucian School)

  • 박천영;김광철
    • 한국가구학회지
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    • 제24권1호
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    • pp.104-112
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    • 2013
  • Monitoring for moisture contents of wooden post member of Un-bong confucian school was performed in 6 months and analyzed to conserve scientifically the wooden culture building and open for common people. In inner post member of the building, variation of moisture contents was very small and upper part had relatively higher moisture contents. In outer post member, moisture contents were changed according to the weather conditions and was relatively high in bottom and upper part. Southern post has lower moisture content than eastern. These results were related to air ventilation in interior, depth of eaves and orientation of the building. In the design, construction and maintenance of wooden building, characteristics of wood should be considered like moisture contents.

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Effects of Saturated Long-chain Fatty Acid on mRNA Expression of Genes Associated with Milk Fat and Protein Biosynthesis in Bovine Mammary Epithelial Cells

  • Qi, Lizhi;Yan, Sumei;Sheng, Ran;Zhao, Yanli;Guo, Xiaoyu
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권3호
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    • pp.414-421
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    • 2014
  • This study was conducted to determine the effects of saturated long-chain fatty acids (LCFA) on cell proliferation and triacylglycerol (TAG) content, as well as mRNA expression of ${\alpha}s1$-casein (CSN1S1) and genes associated with lipid and protein synthesis in bovine mammary epithelial cells (BMECs). Primary cells were isolated from the mammary glands of Holstein dairy cows, and were passaged twice. Then cells were cultured with different levels of palmitate or stearate (0, 200, 300, 400, 500, and 600 ${\mu}M$) for 48 h and fetal bovine serum in the culture solution was replaced with fatty acid-free BSA (1 g/L). The results showed that cell proliferation tended to be increased quadratically with increasing addition of stearate. Treatments with palmitate or stearate induced an increase in TAG contents at 0 to 600 ${\mu}M$ in a concentration-dependent manner, and the addition of 600 ${\mu}M$ was less effective in improving TAG accumulation. The expression of acetyl-coenzyme A carboxylase alpha, fatty acid synthase and fatty acid-binding protein 3 was inhibited when palmitate or stearate were added in culture medium, whereas cluster of differentiation 36 and CSN1S1 mRNA abundance was increased in a concentration-dependent manner. The mRNA expressions of peroxisome proliferator-activated receptor gamma, mammalian target of rapamycin and signal transducer and activator of transcription 5 with palmitate or stearate had no significant differences relative to the control. These results implied that certain concentrations of saturated LCFA could stimulate cell proliferation and the accumulation of TAG, whereas a reduction may occur with the addition of an overdose of saturated LCFA. Saturated LCFA could up-regulate CSN1S1 mRNA abundance, but further studies are necessary to elucidate the mechanism for regulating milk fat and protein synthesis.

예술심리상담사의 감정노동과 삶의 만족도, 일터영성의 매개효과 (The Mediating Effect of Workplace Spirituality on the Relationship between Emotional Labor and Life Satisfaction of Art Psychology Counselors)

  • 이은숙;고정훈
    • 한국산학기술학회논문지
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    • 제22권2호
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    • pp.587-592
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    • 2021
  • 본 연구의 목적은 예술심리상담사의 감정노동과 삶의 만족도와의 관계에서 일터영성의 매개효과를 파악하는데 있다. 연구대상은 속초시 예술심리상담사 196명을 대상으로 실시하였다. 분석방법은 SPSS 프로그램을 사용하여 빈도분석, 신뢰도분석, 상관관계분석, 변수 간의 인과관계 및 매개효과 검증에 대한 회귀분석을 실시하였다. 연구결과 첫째, 예술심리상담사의 감정노동과 삶의 전반적 만족도와의 관계에서 감정노동의 하위요인인 표면행동, 내면행동 요인 모두 삶의 전반적 만족도와의 관계에서 일터영성은 모두 부분 매개효과가 있는 것으로 확인되었다. 둘째, 예술심리상담사의 감정노동과 삶의 주관적 만족도와의 관계에서 감정노동의 하위요인인 내면행동 요인만 삶의 주관적 만족도와의 관계에서 일터영성은 부분 매개효과가 있는 것으로 확인되었다. 이는 예술심리상담사의 감정노동과 삶의 만족도와의 관계에서 일터영성은 매개역할을 하는 것을 의미하는 것이다. 본 연구의 시사점은 예술심리상담사의 삶의 만족도 증진을 위한 방안 마련의 기초자료가 될 것으로 사료된다.

전통마을 생활환경 구성체계와 주생활 특성에 관한 연구 - 임하댐 수몰지역의 수곡동을 대상으로 - (A Study on the Living Environment Organization System in Traditional Village - In the case of the Sugok-dong in the Imha Dam Flooded Area -)

  • 정준현
    • 한국주거학회논문집
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    • 제1권1호
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    • pp.65-76
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    • 1990
  • Sugok Villiage. of the prospective flooded villiages for building Im Ha Dam, is selected as the studied place. Primarily, the purpose of this study is invested, recerded about traditional living environment, and then searched to find out possibilites on the context of the inheritance of analyzing about life contents and space. The result of this study are as follows:1) In composition of living environment, thinking view on confucianism and living view on folk beliefs are considered as important element2) Hierarchy of life environment is appeared of above and below space.(focused on JONG KA) and inner and outer space(boarded on pine forest) on the other hand according to living territory life environment can be divided into five territories.3) Confucian ideas, social status, folk beliefs and economics has been affected about formation of individual life. Accordingly, I believe that true inheritance of traditional culture was, through preserving mental contents as well as physical contents, possible.

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PIE에 기반한 탈북청소년의 문화예술 적응에 대한 연구 (A Study on the Adaptation of Culture and Art Based on PIE for North Korean Adolescents)

  • 김순임;정경열;이영대
    • 문화기술의 융합
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    • 제3권4호
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    • pp.117-122
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    • 2017
  • 탈북청소년이란 북한을 벗어나 남한 사회에 살고 있는 6세 이상 18세 이하의 청소년으로 본 연구에서는 사진활용교육(Photo-In-Education)을 통하여 그들에게 문화예술교육 기회를 제공함으로서 탈북청소년의 피폐해진 심신을 치유하고 남한사회에 정상적으로 적응할 수 있도록 돕는데 연구의 목적이 있다. 새로운 삶에 도전하는 탈북 청소년들에게 '사진 영상 글'을 통하여 자신의 내면세계를 표현하고 그 결과 청소년들은 밝고 맑은 심성을 기르고 표현력, 논리력 등을 개발해 건강하고 똑똑하게 자라도록 힘쓴다. 결과적으로 사회에 대한 긍정적인 감정을 갖게 하고, 더 나아가 사회에 이바지 할 수 있는 건전한 사회인이 되도록 최선을 다하는 것이 본 교육의 의미라고 할 수 있다. 사진 활용교육을 통해 탈북청소년들이 소극적이고 부정적인 그늘에서 벗어나 자신을 긍정적으로 바라보고 자기 내면을 적극적으로 표출하는 것을 알 수 있었으며 PIE가 탈북청소년교육에도 효과적임을 알 수 있었다.

중년기 여성의 차문화 교육요구도 연구 (A Study on the Needs of Tea-Culture Education of Middle-aged Women)

  • 최배영;김영인
    • 가족자원경영과 정책
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    • 제9권3호
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    • pp.69-95
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    • 2005
  • The purpose of this study was to analyze the needs and demands of middle-aged women concerning the educational contents of tea-culture, the managing methods of educational programs, and a plan to invigorate tea culture education as a whole. Here is the summary of the main results. 1) The need for tea-culture education scored an average of 4.14 / 5(maximum score). The actual needs for tea-culture education had varied according to the women's place of living, educational motives, and the desire to become lecturers in the future. That is, the actual needs for tea-culture education scored high among the groups who displayed strong personal motives to cultivate their inner minds and learn tea-culture seriously, groups who lived in Seoul${\cdot}$Taegu${\cdot}$Cheonju, and groups who wanted to become future lecturers. 2) The need for a systematic management of tea-culture educational programs scored an average of 4.10 / 5(maximum score). The need for a management of tea-culture educational programs varied according to the women's educational level and their desire to become future lecturers. That is, the need for a systematic management of tea-culture educational programs scored high among groups whose educational level was high, and groups who wanted to become future lecturers. 3) The need for a plan to invigorate tea-culture education scored an average of 4.05 / 5(maximum score). The need for a plan to invigorate tea-culture education varied according to the women's place of living, educational level, the managing subject of educational organizations, the desire to become future lecturers. That is, the need for a plan to invigorate tea-culture education scored high among the groups who lived in Seoul${\cdot}$Taegu${\cdot}$Cheonju, groups whose educational level was high, groups who were members of life-long educational facilities within universities, and groups who desired to become future lecturers.

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