• Title/Summary/Keyword: Inner Culture Contents

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Concentrations of Calcium-binding Protein and Bone Gla-protein in Culture Medium and CaBP mRNA Expression in Osteoblasts of Broiler Chickens

  • Guo, Xiaoyu;Yan, Sumei;Shi, Binlin;Feng, Yongmiao
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.2
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    • pp.239-245
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    • 2011
  • This study was conducted to determine the effects of excess vitamin A on alkaline phosphatase (ALP) activity, contents of calcium-binding protein (CaBP), bone gla-protein (BGP) in culture medium and CaBP mRNA expression in chicken osteoblasts in vitro. Osteoblastic cells in the tibia from 1-day-old Arbor Acre broiler chickens were isolated using enzyme digestion. The subconfluenced cells were divided into eight treatments with six replicates in each treatment and cultured in a medium containing either vehicle or different levels of vitamin A (0, 0.2, 0.6, 1.0, 2.0, 5.0, 10.0 and $20.0\;{\mu}g$/ml), and the control received an equivalent volume of ethanol. The incubation lasted 48 h. The results showed that vitamin A down-regulated ALP activity in the culture medium as well as CaBP mRNA expression of osteoblasts in a linear dose-dependent manner (p = 0.124 and p<0.10, respectively), and suppressed the contents of BGP and CaBP in the culture medium in a quadratic dose-dependent manner (p<0.05 and p<0.10, respectively) with increasing addition of vitamin A. The addition of 0-$0.2\;{\mu}g$/ml vitamin A to the culture medium increased ALP activity, BGP and CaBP contents as well as CaBP mRNA expression compared with other groups, but positive effects of vitamin A tended to be suppressed when vitamin A was increased to $1.0\;{\mu}g$/ml, and adverse effects occurred when vitamin A was increased to 10.0-$20.0\;{\mu}g$/ml. These results implied that there was a threshold level of vitamin A inclusion beyond which inhibitory effects occurred, and the mechanism by which overdose of vitamin A reduced bone growth in chickens was probably reduced osteoblastic cell activity, and inhibited expression of CaBP mRNA and CaBP secretion.

A Study on the Value Changes for the Korean Women in 1977~1998 -A Content Analysis of Print Ads- (한국여성의 가치관 변화에 관한 연구 -화장품과 의류상품 광고를 중심으로-)

  • 전양진
    • Journal of the Korean Society of Clothing and Textiles
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    • v.25 no.4
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    • pp.773-782
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    • 2001
  • This study was to investigate the value changes in Korean women by analyzing the ads of womens magazines. The contents of ads were classified into two types of values: general and consumption values. The general values, composed of inner-directedness and outer-directrdness, might predict consumer behavior in general. The consumption values, utilitarians and hedonic, were expected to affect the consumer purchasing attitude to a specific product. Factors affecting the cultural values were per capita income and womens social status. Total 2969 illustrated ads with verbal theme from 32 Korean womens magazines were used. The content analysis, chi-square test, logistic regression were done for the analysis. The results showed that inner-directedness was dominant general value in Korean womens culture and increased over time. Younger consumers were more inner-directed than older ones were, and inner-directed values increased with income growth. For the consumption values, utilitarian values were higher than hedonic ones and went up over time. For the product types, utilitarian ads were frequent in cosmetics while hedonic ads were high for apparels. Those results implied that Korean womens culture became more inner-directed with increased income. Also consumption values were likely to differ between product groups. Per capita income was shown to increase inner-directedness while womens social status was to increase outer-directedness.

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A Study on the Comparison and Characteristics of Public Standard Housing Unit Plan before and after the Independence from the Japan Occupation (광복전후(光復前後) 공영주택(公營住宅)의 평면비교(平面比較)와 변화특성(變化特性)에 관한 연구(硏究))

  • Yoo, Jae-Woo
    • Journal of architectural history
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    • v.11 no.2 s.30
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    • pp.23-35
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    • 2002
  • Even though there have been greate changes in the types and contents of the housing plan in Korea since the Modem age, it seems that the identity of traditional dwelling culture is still remains within it. The purpose of this study is to examine the characteristics and features in the development of the 'Public Standard' Housing Unit Plan by comparing the differences and similarities in unit plans in housings built on Japanese occupation period and those built after the period. From the study, we can conclude as follows: 1. The public standard housing unit plans on both periods have shown the common features in the composition of their physical forms in the way that the front-row area still remains the traditional housing culture and the rear-row area reflects the desires of the modem lives. The structure of the dual-row unit plan appeared in the public standard housing plan was caused by the cultural acculturation in Japanese occupation period. 2. The public standard housing unit plans, however, show the differences in the contents and meaning in units plans with the them of the Japanese housing because of the cultural differences. These differences took placed autonomously through the change of housing plans in the modern time, and have the common features such that 1) the changes in the Maru-centered plan type differ in composition from the inner-corridor plan type used Japanese housing, and 2) the composition of the open-inner spaces in the double-row housing plan reflects the single-row outer open space In the traditional housing. All of these cultural progresses in the public standard housing unit plans was took place as the result of the inner-oriented and notional circulations derived from the modernity. However, the transformed unit plans still contains the identity derived from the variation of the traditional Korean building elements such as Maru and Ondol. In other words, those are the Maru-centered and composed housing plans with juxtaposition of the inner block and the outer block. As the conclusion, though the public standard dwelling unit plan has the similarity in the physical form, it is different in the cultural Identity and meaning from the public standard plans before the independence from the Japan occupation.

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The Development and Application of Identity Design to Facilitate Farm Restaurants - Developing menu of barley dishes for Hwanggeum Bori (golden barley) in Gimje, North Jeolla Province (농가맛집의 활성화를 위한 아이덴티티 디자인 개발과 적용 - 전북 김제 황금보리 밥상개발을 예로 -)

  • Chang, Hea Jin;Kim, Su In
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.9 no.4
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    • pp.143-153
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    • 2013
  • The rural tourism in Korea is expanding in scope including experience tours and integrating with dining industry these days. The Rural Development Administration in Korea has been supporting the farm restaurant project across the nation as part of its efforts to utilize local or rural food recipes as tourism resource for seven years. Over the years, however, case studies are rarely done or reported regarding physical environment of farm restaurants. This study offers an opportunity for farm restaurateurs and policy makers related to recognize the importance of developing brand identity and inner culture contents for the rural community by presenting a case of integrated design marketing. In the study, definitions of the "authentic food" in Korea and other countries are compared before farm restaurants at home and abroad are investigated. It also addresses the foundation on the significance of physical environment and design to build a brand identity of authentic food. The case presented is naming, designing and developing logos and design applicable to various product packages for "Hwanggeum Bori Aechan", which is the barley (main crop in Gimje)- dish development project for Gimje, North Jeolla Province in 2012.

Study on Moisture Contents Distribution of Wooden Post Member by Precise Monitoring in Un-Bong Confucian School (운봉 향교 정밀 모니터링을 통한 목재 기둥 부재의 함수율 분포 특성)

  • Park, Chun-Young;Kim, Gwang-Chul
    • Journal of the Korea Furniture Society
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    • v.24 no.1
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    • pp.104-112
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    • 2013
  • Monitoring for moisture contents of wooden post member of Un-bong confucian school was performed in 6 months and analyzed to conserve scientifically the wooden culture building and open for common people. In inner post member of the building, variation of moisture contents was very small and upper part had relatively higher moisture contents. In outer post member, moisture contents were changed according to the weather conditions and was relatively high in bottom and upper part. Southern post has lower moisture content than eastern. These results were related to air ventilation in interior, depth of eaves and orientation of the building. In the design, construction and maintenance of wooden building, characteristics of wood should be considered like moisture contents.

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Effects of Saturated Long-chain Fatty Acid on mRNA Expression of Genes Associated with Milk Fat and Protein Biosynthesis in Bovine Mammary Epithelial Cells

  • Qi, Lizhi;Yan, Sumei;Sheng, Ran;Zhao, Yanli;Guo, Xiaoyu
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.3
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    • pp.414-421
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    • 2014
  • This study was conducted to determine the effects of saturated long-chain fatty acids (LCFA) on cell proliferation and triacylglycerol (TAG) content, as well as mRNA expression of ${\alpha}s1$-casein (CSN1S1) and genes associated with lipid and protein synthesis in bovine mammary epithelial cells (BMECs). Primary cells were isolated from the mammary glands of Holstein dairy cows, and were passaged twice. Then cells were cultured with different levels of palmitate or stearate (0, 200, 300, 400, 500, and 600 ${\mu}M$) for 48 h and fetal bovine serum in the culture solution was replaced with fatty acid-free BSA (1 g/L). The results showed that cell proliferation tended to be increased quadratically with increasing addition of stearate. Treatments with palmitate or stearate induced an increase in TAG contents at 0 to 600 ${\mu}M$ in a concentration-dependent manner, and the addition of 600 ${\mu}M$ was less effective in improving TAG accumulation. The expression of acetyl-coenzyme A carboxylase alpha, fatty acid synthase and fatty acid-binding protein 3 was inhibited when palmitate or stearate were added in culture medium, whereas cluster of differentiation 36 and CSN1S1 mRNA abundance was increased in a concentration-dependent manner. The mRNA expressions of peroxisome proliferator-activated receptor gamma, mammalian target of rapamycin and signal transducer and activator of transcription 5 with palmitate or stearate had no significant differences relative to the control. These results implied that certain concentrations of saturated LCFA could stimulate cell proliferation and the accumulation of TAG, whereas a reduction may occur with the addition of an overdose of saturated LCFA. Saturated LCFA could up-regulate CSN1S1 mRNA abundance, but further studies are necessary to elucidate the mechanism for regulating milk fat and protein synthesis.

The Mediating Effect of Workplace Spirituality on the Relationship between Emotional Labor and Life Satisfaction of Art Psychology Counselors (예술심리상담사의 감정노동과 삶의 만족도, 일터영성의 매개효과)

  • Lee, EunSook;Ko, JeongHoon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.2
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    • pp.587-592
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    • 2021
  • The purpose of this study is to grasp the mediating effect of workplace spirituality on the relationship between emotional labor and life satisfaction of art psychology counselors. The subjects of the study included 196 art psychological counselors in Sokcho city. As an analysis method, the SPSS program was used to perform frequency analysis, reliability analysis, correlation analysis, and regression analysis for the causal relationships between variables and the verification of the mediating effect. First, for the relationship between the emotional labor of art psychology counselors and their overall life satisfaction, both the surface behavior and inner behavior factors, which are sub-factors of emotional labor, have partial mediating effects on overall life satisfaction. Second, in the relationship between the emotional labor of art psychology counselors and their subjective life satisfaction, the inner behavioral factor, which is a sub-factor of emotional labor, has a partial mediating effect in relation to workplace spirituality. This means that workplace spirituality plays a mediating role in the relationship between emotional labor and life satisfaction of art psychology counselors. This study is considered as basic data for preparing plans for improving the life satisfaction of artistic psychological counselors.

A Study on the Living Environment Organization System in Traditional Village - In the case of the Sugok-dong in the Imha Dam Flooded Area - (전통마을 생활환경 구성체계와 주생활 특성에 관한 연구 - 임하댐 수몰지역의 수곡동을 대상으로 -)

  • 정준현
    • Journal of the Korean housing association
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    • v.1 no.1
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    • pp.65-76
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    • 1990
  • Sugok Villiage. of the prospective flooded villiages for building Im Ha Dam, is selected as the studied place. Primarily, the purpose of this study is invested, recerded about traditional living environment, and then searched to find out possibilites on the context of the inheritance of analyzing about life contents and space. The result of this study are as follows:1) In composition of living environment, thinking view on confucianism and living view on folk beliefs are considered as important element2) Hierarchy of life environment is appeared of above and below space.(focused on JONG KA) and inner and outer space(boarded on pine forest) on the other hand according to living territory life environment can be divided into five territories.3) Confucian ideas, social status, folk beliefs and economics has been affected about formation of individual life. Accordingly, I believe that true inheritance of traditional culture was, through preserving mental contents as well as physical contents, possible.

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A Study on the Adaptation of Culture and Art Based on PIE for North Korean Adolescents (PIE에 기반한 탈북청소년의 문화예술 적응에 대한 연구)

  • Kim, Soonim;Jeong, Gyoungyoul;Lee, Youngdae
    • The Journal of the Convergence on Culture Technology
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    • v.3 no.4
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    • pp.117-122
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    • 2017
  • North Korean youth is a youth aged 6 to 18 who lives in a South Korean society after having left North Korea. In this study, photo-in-education (PIE) provides North Korean youths with opportunities for cultural and arts education, and to help them adjust themselves to South Korean society. As a result, they, as young people who are exploring a new life in South Korea, have been able to express their own inner world through photos, videos, and writings. Also, young people have developed a bright and clear mind, and enhanced an expressive power and logical power that helps grow healthily and intelligently. Further, it is the aim of this education to help them to have a positive feeling for society and to do their best to become a healthy member of society, who will be able to contribute to society. Through PIE, it was found that North Korean defectors were able to see themselves positively and express their own inner self positively, despite that they are still in the negative and shady side of life.

A Study on the Needs of Tea-Culture Education of Middle-aged Women (중년기 여성의 차문화 교육요구도 연구)

  • Choi Bae-Young;Kim Young-In
    • Journal of Family Resource Management and Policy Review
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    • v.9 no.3
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    • pp.69-95
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    • 2005
  • The purpose of this study was to analyze the needs and demands of middle-aged women concerning the educational contents of tea-culture, the managing methods of educational programs, and a plan to invigorate tea culture education as a whole. Here is the summary of the main results. 1) The need for tea-culture education scored an average of 4.14 / 5(maximum score). The actual needs for tea-culture education had varied according to the women's place of living, educational motives, and the desire to become lecturers in the future. That is, the actual needs for tea-culture education scored high among the groups who displayed strong personal motives to cultivate their inner minds and learn tea-culture seriously, groups who lived in Seoul${\cdot}$Taegu${\cdot}$Cheonju, and groups who wanted to become future lecturers. 2) The need for a systematic management of tea-culture educational programs scored an average of 4.10 / 5(maximum score). The need for a management of tea-culture educational programs varied according to the women's educational level and their desire to become future lecturers. That is, the need for a systematic management of tea-culture educational programs scored high among groups whose educational level was high, and groups who wanted to become future lecturers. 3) The need for a plan to invigorate tea-culture education scored an average of 4.05 / 5(maximum score). The need for a plan to invigorate tea-culture education varied according to the women's place of living, educational level, the managing subject of educational organizations, the desire to become future lecturers. That is, the need for a plan to invigorate tea-culture education scored high among the groups who lived in Seoul${\cdot}$Taegu${\cdot}$Cheonju, groups whose educational level was high, groups who were members of life-long educational facilities within universities, and groups who desired to become future lecturers.

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