• Title/Summary/Keyword: Inje gun

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Electron Microscopic Studies on Distribution of Collagen IV of Lens Capsule and Apoptosis of Lens Epithelium in Age-related Cataractous Human (백내장 환자의 연령에 따른 수정체낭 Type IV 아교섬유의 분포와 수정체낭 상피세포 자연사 및 전자현미경적 변화에 대한 연구)

  • Heo, Jun;Yang, Young-Chul;Won, In-Gun
    • Biomedical Science Letters
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    • v.4 no.2
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    • pp.77-86
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    • 1998
  • Age-related changes in the lens capsule and epithelium of cataractous patients, ranging from 20 to 7o years old, were studied by means or LM, immunohistochemistry, and TEM. The lens capsule was divided into four zones; the anterior, subanterior, middle, and basal zone. The van Gieson staining reaction for collagen was prominent at the anterior and subanterior parts of the lens capsule. The reaction was more decreased in the elder group than the younger group. The collagen type IV reaction was prominent at the anterior zone of the lens capsule and around the cell. The reaction was more decreased in the elder group than the younger group. 3. The Periodic Acid Shiff-Alcian Blue reaction for mucopolysaccharide was prominent at the anterior zone of the lens capsule. The reaction was more decreased in the elder group than the younger group. The Apoptotic reaction was prominent at the nucleus of the lens epithelial cell. In the elder the cataractous group, the number of the apoptotic cells was more decreased. The electron microscopic change of lens epithelial cells were characterized by the increase of lateral fold and the cytoplasm with various vacuoles and Golgi complex. In the basal part, lens epithelial cell protruded toward the lens capsule in the 20-year-old group. The basal part of the 40-year-old group was flattened and covered with the cytoplasmic processes of adjacent cells. In the 60-year-old group, the mass of rough filaments separated lens capsule and the basal part of the lens epithelial cell. The electron microscopic change of the middle part of lens capsule was characterized by the aggregation of electron dense materials in the 40-year-old group, and the appearance of filamentous materials and the decrease of electron dense granules in the 60-year-old group.

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A Study on the Medical Services Satisfaction of Cancer Patients in Busan Area (부산지역 암환자의 의료서비스 만족도에 관한 연구)

  • Kim, Byeong-Gun;Yang, Jong-Hyun;Chang, Dong-Min
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.1
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    • pp.236-246
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    • 2012
  • Hospital out-shopping by local patients has become more popular. In particular, many cancer patients have visited hospitals in the capital area to get better medical services regardless of how far the hospitals are from their home. However this kind of hospital out-shopping may discontinue medical treatment and cause a waste of medical resources including manpower. In addition, it creates additional economic and social expenses such as caretakers' transportation and lodging expenses. It is necessary to study patients who would take the medical treatment in Busan go to Seoul for better medical services are more satisfied based on objective data. For this, a questionnaire survey has been performed against 223 cancer patients who had a surgery in Busan and 187 patents who had an operation in Seoul. According to the survey, the patients who had an operation in the capital area(Group A) were more satisfied than the patients who had a surgery in Busan(Group B) in terms of convenience facilities, doctors' competence. However the Group A was very dissatisfied in terms of accessibility and satisfaction on overall medical services.

A Major Antioxidative Components and Comparison of Antioxidative Activities in Black Soybean (검정콩의 주요 항산화 원인물질 및 항산화 효과의 비교)

  • Kim, Sun-Hee;Kwon, Tai-Wan;Lee, Young-Soon;Choung, Myoung-Gun;Moon, Gap-Soon
    • Korean Journal of Food Science and Technology
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    • v.37 no.1
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    • pp.73-77
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    • 2005
  • Contents of isoflavone, phenolic acids, tocopherol, and anthocyanin in black soybean (Glycine max) were measured by HPLC. To compare antioxidative activities of main black soybean components, antioxidative effects of the same levels of commercial standard components were measured by Trolox equivalent antioxidant capacity assay (TEAC). Most effective component was gentisic acid followed by anthocyanin, p-coumaric acid, ferulic acid, genistein, syringic acid, and daidzein. TEAC assay results revealed genistein in isoflavone, gentisic acid in phenolic acids, p-tocopherol in tocopherol, and anthocyanin showed highest antioxidative and synergistic acitivities, with anthocyanin showing strongest synergy effect.

Flora of Natural Mashes in Wondae-ri (Inje-gun, Kangwon-do) (강원도 인제군 원대리 자연습지의 식물상)

  • Park, Wan-Geun;Yoo, Seok-In;Park, Kwang-Seo
    • Journal of Forest and Environmental Science
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    • v.16 no.1
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    • pp.50-68
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    • 2000
  • This study was to estabilish the composition and vegetation of vascular plants on natural mashes in Wondae-ri(710m: $37^{\circ}$ 58' 30" N, $128^{\circ}$ 12' 15" E), This work was conducted from April to October, 1999. Vascular plants were composed of 71 families, 175 genera, 228 species, 1 subspecies, 33 varieties and 3 formae, totaling 265 taxa. Among the investigated vascular plants, 19 families, 24 genera, 31 species, 5 varieties, 1 formae, totaling 37 taxa (13.9%) were hydrophytes and hygrophytes. The forest vegetation was classified into 1 community and 2 subcommunities ; A. Phragmites japonica community A-1. Salix gracilistyla-Impatiens noli-tangere subcommunity A-2. Spiraea prunifolia var. simpliciflora-Artemisia capillaris subcommunity.

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Production of Cheonggukjang by Using a Recombinant Bacillus licheniformis Strain

  • Jeong, Woo-Ju;Kwon, Gun-Hee;Lee, Ae-Ran;Park, Jae-Yong;Lee, Mee-Ryung;Chun, Ji-Yeon;Cha, Jae-Ho;Song, Young-Sun;Kim, Jeong-Hwan
    • Preventive Nutrition and Food Science
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    • v.14 no.1
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    • pp.90-93
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    • 2009
  • Cheonggukjang was prepared from soybean inoculated with B. licheniformis ATCC 10716 cells transformed with pHY3-5 carrying a fibrinolytic enzyme gene. During the 54 hr of fermentation at $37^{\circ}C$, fibrinolytic activities of cheonggukjang were significantly higher than cheonggukjang fermented with B. licheniformis 10716 control cells. The plasmid, pHY3-5 was stably maintained during the 54 hr without antibiotic selection and more than 52% of cells retained the plasmid.

Cloning of aprE86-1 Gene Encoding a 27-kDa Mature Fibrinolytic Enzyme from Bacillus amyloliquefaciens CH86-1

  • Lee, Ae-Ran;Kim, Gyoung-Min;Kwon, Gun-Hee;Lee, Kang-Wook;Park, Jae-Yong;Chun, Ji-Yeon;Cha, Jae-Ho;Song, Young-Sun;Kim, Jeong-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.20 no.2
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    • pp.370-374
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    • 2010
  • A gene encoding the major secreted fibrinolytic protein of Bacillus amyloliquefaciens CH86-1 was cloned from genomic DNAs. DNA sequencing showed that the gene, aprE86-1, could direct the synthesis of a mature protein 275 amino acids in length after processing. When aprE86-1 was introduced into B. subtilis, a mature 27-kDa protein was produced as expected. The fibrinolytic activity of the B. subtilis transformant (TF) was higher than that of B. amyloliquefaciens CH86-1, showing the possibility of increasing the fibrinolytic activity of Bacillus strains through genetic engineering.

Ecological Characteristics and Distribution of Plant Resources of Pyrus and Malus sp. in Jindong Valley, Gangwon Province

  • Ahn, Young-Hee;Chung, Kyu-Hwan;Choi, Kwang-Yool;Park, Dae-Sik
    • Plant Resources
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    • v.4 no.3
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    • pp.130-139
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    • 2001
  • Four major naturally populated areas around the Jindong valley in Inje-Gun, Gangwon province for wild Pyrus ussuriensis, Malus sieboldii, and Malus baccata are mostly located on the southeast side of the mountains at 630-745m in altitude at sea level and are in very good sunny areas. The total of 77 taxa dividing into 32 families, 58 genera, 65 species, and 12 varieties has been inhabited in these areas. Most of these plants are heliophytes, which love sunshine, but some of rhizo-plants such as Sasa borealis, climbing plants such as Actinidia arguta and Celastrus orbiculatus, or naturalized plants like Bidens frondosa are also shown. These areas have been continually destroyed, so it is possibly thought that the second transition has been progressed. The index of species diversity of plant groups in these areas is 0.672∼1.465 based on the Shannon-Wiener's method, but its index for the area that rhizo-plants like Sasa borealis are growing as an under planting decreases relatively. In this study, the oldest plant of Pyrus ussuriensis in Korea, which is 120cm in diameter at breast height (DBH), 19m in tree height and 25m in width, has been found.

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Electrodermal Activity at Palms according to Pressure Stimuli applied to the Scapula

  • Kim, Jae-Hyung;Park, Gun-Cheol;Baik, Sung-Wan;Jeon, Gye-Rok
    • Journal of Korea Multimedia Society
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    • v.19 no.7
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    • pp.1137-1145
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    • 2016
  • The system for measuring the electrodermal activity (EDA) signal occurring at the sweet glands in the human body was implemented in this study. The EDA measurement system (EDAMS) consisted of an algometer and the bio-potential measurement system (BPMS). Three experiments were performed using EDAMS. First, the linearity of the output voltage corresponding to the pressure being applied to an algometer was evaluated. The linearity of output voltage according to the pressure was 0.956. Second, the amplitude and the latency of the EDA signal at the left palm was obtained while applying the pressure stimuli to the left and right scapula. The latency of EDA signal was shorter whereas the amplitude of EDA signal was higher when the pressure applied was applied to the left scapula. Third, the amplitude and latency of the EDA was measured at left and right palm while increasing the pressure stimuli to the left scapula. The latency of EDA signal at left and right palm was decreased according to the intensity of pressure stimulus applied to the left scapula. However, the latency of the EDA signals did not show the linearity with respect to the pressure stimuli.

Effects of Ca Addition on Grain Refinement and Mechanical Properties of AZ31 Magnesium Alloy (AZ31 마그네슘합금의 결정립 미세화 및 기계적 특성에 미치는 Ca 첨가의 영향)

  • Jin, Qing-Lin;Eom, Jeong-Pil;Lim, Su-Gun;Park, Won-Wook;You, Bong-Sun
    • Journal of Korea Foundry Society
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    • v.23 no.5
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    • pp.251-256
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    • 2003
  • Effects of Ca addition on grain refinement, microstructure and mechanical properties of AZ31 Mg alloy were investigated. Due to Ca addition to Mg alloy (AZ31), the microstructure was refined, the quantity of $Mg_{17}Al_{12}$phase was reduced, and new $Al_2Ca$ phase was formed. The tensile property of AZ31 was increased with the minor addition of Ca, but was decreased rapidly over 0.2 wt.% of Ca content. The $Al_2Ca$ phase was considered to be detrimental to the mechanical property of AZ31 Mg alloy.

Heterologous Gene Expression of aprE2 Encoding a 29 kDa Fibrinolytic Enzyme from Bacillus subtilis in Bacillus licheniformis ATCC 10716

  • Kwon, Gun-Hee;Jeong, Woo-Ju;Lee, Ae-Ran;Park, Jae-Yong;Cha, Jae-Ho;Song, Young-Sun;Kim, Jeong-Hwan
    • Food Science and Biotechnology
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    • v.17 no.6
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    • pp.1372-1375
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    • 2008
  • The aprE2 gene from Bacillus subtilis CH3-5 was expressed in Bacillus licheniformis ATCC 10716 using a Bacillus-Escherichai coli shuttle vector, pHY300PLK. The fibrinolytic activity of transformant (TF) increased significantly compared to B. licheniformis 10716 control cell. During the 100 hr incubation in Luria-Bertaini broth at $37^{\circ}C$, fibrinolytic activity of B. licheniformis TF increased rapidly at the late growth stage, after 52 hr of incubation, which was confirmed by zymography using a fibrin gel. pHY3-5 was stably maintained in B. licheniformis without tetracycline (Tc) in the media, 60.9% of cells still maintained pHY3-5 after 100 hr of cultivation.