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Heterologous Gene Expression of aprE2 Encoding a 29 kDa Fibrinolytic Enzyme from Bacillus subtilis in Bacillus licheniformis ATCC 10716  

Kwon, Gun-Hee (Division of Applied Life Science (BK21 Program), Gyeongsang National University)
Jeong, Woo-Ju (Division of Applied Life Science (BK21 Program), Gyeongsang National University)
Lee, Ae-Ran (Division of Applied Life Science (BK21 Program), Gyeongsang National University)
Park, Jae-Yong (Institute of Agriculture and Life Science, Gyeongsang National University)
Cha, Jae-Ho (Department of Microbiology, Pusan National University)
Song, Young-Sun (School of Food and Life Science, Inje University)
Kim, Jeong-Hwan (Division of Applied Life Science (BK21 Program), Gyeongsang National University)
Publication Information
Food Science and Biotechnology / v.17, no.6, 2008 , pp. 1372-1375 More about this Journal
Abstract
The aprE2 gene from Bacillus subtilis CH3-5 was expressed in Bacillus licheniformis ATCC 10716 using a Bacillus-Escherichai coli shuttle vector, pHY300PLK. The fibrinolytic activity of transformant (TF) increased significantly compared to B. licheniformis 10716 control cell. During the 100 hr incubation in Luria-Bertaini broth at $37^{\circ}C$, fibrinolytic activity of B. licheniformis TF increased rapidly at the late growth stage, after 52 hr of incubation, which was confirmed by zymography using a fibrin gel. pHY3-5 was stably maintained in B. licheniformis without tetracycline (Tc) in the media, 60.9% of cells still maintained pHY3-5 after 100 hr of cultivation.
Keywords
Bacillus licheniformis; fibrinolytic enzyme; gene expression; transformation; Bacillus subtilis;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
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