• 한국식품영양과학회지
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• 제35권2호
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• pp.158-163
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• 2006

유황처리 토양에서 재배한 열무 YR-A와 유황처리 후 복토를 덮어 재배한 열무 YR-B와 이 열무로 김치를 담근김치 A와 김치 B의 pH및 산도와 AGS 인체 위암세포의 성장억제효과에 미치는 영향을 관찰하였다. YR-A로 담근 열무김치 A와 YR-B로 담근 김치 B는 $4\~5$주일째에 적숙기의 pH에 도달하였으며, 이 때 산도는 $0.88\~1.20\%$의 높은 값을 나타내었다. 열무김치 A와 B는 발효가 더욱 진행되어 $6\~8$주일째가 되어도 pH는 4.0이 하로 감소하지 않고 저장성이 길어지는 경향을 나타내었다. 유황처리한 김치 A와 다시 복토를 한 김 치 B는 일반 토양에서 재배한 대조군 김치에 비해 다소 빠른 시일에 적숙기에 도달하였다. 발효초기에는 김치 A가 대조군 김치보다 높은 Leuconostoc속 젖산균 함량을 나타내었으나, Lactobacillus속 젖산균수는 낮은 함량을 나타내었다. 암세포 성장억제효과의 경우, 유황처리 열무 YR-A 즙액 과 VR-B 즙액은 일반토양에서 재배한 YR-Control 즙액에 비해 유의적으로 $70\%$의 높은 암세포 성장억제효과를 나타내었으며, YR-A와 YR-B는 유의적 차이가 없었다. 대조군 김치, 김치 A와 김치 B의 각 즙액은 첨가 농도 $10{\mu}L/assay$에서는 암세포 성장억제효과가 거의 나타나지 않았으나, 첨가 농도 $20{\mu}L/assay$에서 는 약 $74\%$의 AGS 위 암세포에 대한 성장억제효과가 있었으며 이 결과는 열무의 즙액시료 YR-A와 YR-B에서와 비슷한 효과를 나타내는 것으로 보아 열무김치의 발효과정에서는 그 효과가 거의 나타나지 않았음을 알 수 있었다. 열무김치의 메탄올추출물을 이용하여 암세포의 성장억제효과를 관찰한 결과에서도 거의 비슷한 경향을 나타내었다. 따라서 열무 재배시 토양에의 유황처리방법은 열무김치의 발효과정 및 적숙기에서 시원한 맛을 증가시키고 신맛을 감소시켜 유황처리로 재배한 열무김치가 맛 부분에서 다소 우수하다고 할 수 있으나, 유황처리한 열무 YR-A와 다시 복토를 한 열무 YR-B의 산도와 맛에는 별로 차이가 없었다. 또 유황처리토양에서 재배된 열무와 이 열무로 담근 김치즙액의 AGS 암세포 성장 저지효과는 거의 비슷한 수준이었으며, 유황을 첨가하여 재배한 열무의 경우 대조군에 비해 그 효과가 증진되었다. 이 결과에 의하여 유황을 첨가하여 재배한 열무의 암세포의 성장을 저지하는 활성 성분의 증가가 예상되어진다. 본 연구결과로부터 유황처리 열무의 기능성 식품재료로서의 개발가능성을 기대할 수 있으리라 생각된다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 춘계학술발표회
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• pp.318-319
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• 2011

• 대한한방부인과학회지
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• 제21권2호
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• pp.125-151
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• 2008

Purpose: In this study, we investigated the anti-thrombotic efficacy of "Eungapbang-gagam(EGB)" currently used in clinical treatment of PID Methods: We studied inhibitory effect of platelet cohesion, suppressive effect of GPIIb/IIIa activity, inhibitory effect of $TXB_2$ and $PGE_2$ biosynthesis, and oxidative damage suppression effects of "EGB" in vitro. Also, suppression of pulmonary embolism and changes of related factors in dextran coagulation condition model were studied in vivo. Results: In this study, EGB extract showed dose-dependent inhibitory effect on platelet coagulation induced by ADP, epinephrine, collagen, arachidonic acid. Also it showed dose-dependent inhibition effect on GPIIb/IIIa activities compared to the control group. EGB extract significantly suppressed the decrease of speed of bloodstream caused by blood coagulation in dextran coagulation condition model and increased the number of platelets and amount of fibrinogen, and decreased the APTT in dextran coagulation condition model compared to the control group. EGB extract showed dose-dependent decrease of oxidative damages caused by DPPH and superoxide anion radicals, whereas dose-dependent increase of superoxide dismutase like activity was observed compared to the control group. Conclusion: We confirmed the anti-thrombosis and anti-oxidative efficacy of "Eungapbang-gagam". Various clinical applications of "Eungapbang-gagam" as well as use of data for the construction of EBM is anticipated.

• 한국식품영양학회지
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• 제28권4호
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• pp.635-642
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• 2015

This study was performed to investigate the physiological functionality of jerusalem artichokes and to define its antioxidant potential. The study also evaluated mook prepared with jerusalem artichokes powder as a new functional food for ameliorating Diabetes Mellitus. Regarding the chromaticity of mook prepared with jerusalem artichoke, lightness decreased as the amount of jerusalem artichoke increased (p < 0.05), while the redness and yellowness values increased significantly (p < 0.05). Regarding the mechanical properties, hardness, springiness, chewiness, gumminess, and cohesiveness decreased significantly as the amount of jerusalem artichoke increased (p < 0.05). The total polyphenol and flavonoid contents of JA30 were 7.36 mg TAE/g and 2.15 mg RE/g, respectively, which were higher than those of the control group(3.50 mg TAE/g and 0.76 mg RE/g, respectively). The DPPH and ABTS scavenging abilities ($IC_{50}$) for JA30 were 4.40 and 5.70 mg/mL, respectively, indicating a higher radical scavenging ability than the control group (7.59 and 7.24 mg/mL, respectively). The ${\alpha}-glucosidase$ inhibitory effect ($IC_{50}$) of JA30 and the control group was 18.91 and 24.01 mg/mL, respectively. Based on the above-described results, mook containing jerusalem artichoke powder is expected to ameliorate diabetes with antioxidant activity, ${\alpha}-glucosidase$ inhibitory effect, and sensory factor. Also, it is believed that this functional material has the potential to improve health.

• 농업과학연구
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• 제44권3호
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• pp.339-347
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• 2017

Sclerotium rolfsii causing southern blight on numerous vegetable and fruit crops was isolated from stems of chili peppers showing wilting symptoms. The pathogen was identified by morphological observation and DNA sequencing analysis of ITS region. To select an effective fungicide for control of southern blight, we investigated the inhibition efficacy of thirty fungicides included in nine groups of fungicides with different mechanisms of action. A fungal growth inhibition assay was conducted through an agar dilution method by using mycelial discs and sclerotia of the pathogen as inoculum, respectively. When mycelial discs were used as an inoculum, several fungicides showed good inhibitory activity against the mycelial growth of S. rolfsii 12-6. All DMI fungicides tested had a good inhibition except for prochloraz which had low inhibitory effect. All strobilurin fungicides tested except for kresoxim-methyl and all SDHI fungicides tested except for boscalid and fluopyram, had a good inhibition. Also, fludioxonil, a protective fungicide and fluazinam had a good inhibitory effect. Interestingly, when sclerotia were used as an inoculum, inhibition efficacy was increased for fluopyram, a SDHI fungicide, and for some protective fungicides such as propineb, chlorothalonil, dithianon, and folpet. All the fungicides selected in this study should be tested in the field for their control activities against stem rot for practical use in chili pepper cultivation.

• 한방안이비인후피부과학회지
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• 제37권1호
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• pp.17-28
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• 2024

Objectives : This study was conducted to reveal the scientific mechanism of the anti-skin aging activity of Kyung-Ok-Ko(KOK), which is highly useful as a Korean traditional medicine and functional food. Methods : The skin wrinkle and aging inhibitory activity of KOK was confirmed through in vitro experiments of human dermal fibroblast neonatal cell(HDFn) and in vivo of C. elegans, and hairless mouse(SKH-1). Results : The amount of the C-terminus of the collagen precursor in the HDFn cell culture medium treated with KOK using an enzymes-linked immunoassay kit. The group treated with KOK 200㎍/㎖ was a 28.3% increase of collagen precursor compared to the control group. KOK showed inhibitory activity of MMP-1 compared to the control group at a concentration of 200㎍/㎖. In addition, KOK 200㎍/㎖ showed significant inhibitory activity of thermal stress and an oxidative stress compared to the control group in C. elegans. Furthermore, KOK showed a concentration-dependent(100mg/kg and 500mg/kg) anti-wrinkle formation effect in UV-irradiated hairless mouse(SKH-1). Additionally, when KOK was administered to UV-irradiated hairless mice, an increase in procollagen -1 and -3 genes expression was observed, and mmp-1 and mmp-9 genes, which increase collagen decomposition, decreased with the administration of KOK. Conclusions : The skin aging inhibition mechanism of Kyung-Ok-Ko(KOK) is presumed to be achieved through suppressing thermal stress and oxidative stress, suppressing mmp-1 and mmp-9 genes, and increasing procollagen-1 and procollagen-3.

• Animal cells and systems
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• 제3권2호
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• pp.103-113
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• 1999

Many in-depth reviews related to regulations of prolactin secretion are available. We will, therefore, focus on controversial aspects using personal opinion in this review. The neuroendocrine control of prolactin secretion from the anterior pituitary gland involves multiple factors including prolactin-release inhibiting factor (PIF) and prolactin releasing factor (PRF). The PIF exerts a tonic inhibitory control in the physiological conditions. The PIF should be able to effectively inhibit prolactin release or a lifetime, but the inhibitory action of dopamine cannot be sustained for a long period of time. Perifusion of a high concentration of dopamine (l ,000 nM) could not sustain inhibitory action on prolactin release but when a small amount of ascorbic acid (0.1 mM) is added in a low concentration of dopamine (3 nM) solution, prolactin release was inhibited for a long period. Ascorbate is essential for dopamine action to inhibit prolactin release. We have, therefore, concluded that the PIF is dopamine plus ascorbate. The major transduction system for dopamine to inhibit prolactin release is the adenylyl cyclase system. Dopamine decreases cyclic AMP concentration by inhibiting adenylyl cyclase, and cyclic AMP stimulates prolactin release. However, the inhibitory mechanism of dopamine on prolactin release is much more complex than simple inhibition of CAMP production. The dopamine not only inhibits cyclic AMP synthesis but also inhibits prolactin release by acting on a link(s) after the CAMP event in a chain reaction for inhibiting prolactin release. Low concentrations of dopamine stimulate prolactin release. Lactotropes are made of several different subtypes of cells and several different dopamine receptors are found in pituitary. The inhibitory and stimulatory actions induced by dopamine can be generated by different subtype of receptors. The GH$_4$ZR$_7$ cells express only the short isoform (D$_{2s}$) of the dopamine receptor, as a result of transfecting the D$_{2s}$ receptors into GH$_4$C$_1$ cells which do not express any dopamine receptors. When dopamine stimulates or inhibits prolactin release in GH$_4$ZR$_7$ cells, it is clear that the dopamine should act on dopamine D$_{2s}$ receptors since there is no other dopamine receptor in the GH$_4$ZR$_7$. Dopamine is able to stimulate prolactin release in a relatively low concentration while it inhibits in a high concentration in GH$_4$ZR$_7$. These observations indicate that the dopamine D$_2$ receptor can activate stimulatory and/or inhibitory transduction system depending upon dopamine concentrations.

• 대한한방내과학회지
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• 제24권1호
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• pp.44-54
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• 2003

Objectives : This study was done to investigate the protective effects of Gagaminjakdowha-Tang on liver injury of rats induced by $CCl_4$ and d-galactosamine. Methods : All animals were divided into 5 groups, those were normal group(untreated), control group(treated with 0.9% Saline solution), sample I group(200mg/kg administrated), sample II group(400mg/kg administrated), Silymarin(200mg/kg administrated) group. Liver injury of rats were induced by $CCl_4$ and d-galactosamine, and then the serumtransaminase(ALT & AST) alkaline phosphatase(ALP), lactic dehydrogenase(LDH) for enzyme activities, Liver cytosol malondialdehyde(MDA), catalase, superoxide dismutase(SOD), glutathione S-transferase(GST) and glutathione-peroxidase(GPX) for enzyme activities were measured. Results : The inhibitory effects on the serum ALT activities were noted in both sample I and sample II group. The inhibitory effects on the serum AST activities were noted in only sample II group. The inhibitory effects on the serum ALP activities were noted in both sample I and sample II group. The inhibitory effects on the serum LDH activities were noted in only sample II group. The inhibitory effects on the liver cytosol malondialdehyde were noted in only sample II group. The decresed effects on the liver cytosol catalase activities were inhibited in only sample II group. The decresed effects on the liver cytosol superoxide dismutase activities were inhibited in only sample II group. The decresed effects on the liver cytosol GST activities were inhibited in only sample II group. The decresed effects on the liver cytosol GPX activities were inhibited in only sample II group. The inhibitory effects of the serum ALT activities were noted in both sample I and sample II. The inhibitory effects of the serum AST activities were noted in only sample II group. The inhibitory effects of the serum ALP activities were noted in only sample II group. The inhibitory effects of the serum LDH activities were noted in both sample I and sample II group. Conclusions : Gagaminjakdowha-Tang has protective effects against liver injury in rats induced by $CCl_4$ and d-galactosamine.

• Asian Pacific Journal of Cancer Prevention
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• 제15권7호
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• pp.3175-3178
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• 2014

Background: Gastric cancer is a common malignant tumor. Our previous study demonstrated inhibitory effects of 3-bromopyruvate (3-BrPA) on pleural mesothelioma. Moreover, we found that 3-BrPA could inhibit human gastric cancer cell line SGC-7901 proliferation in vitro, but whether similar effects might be exerted in vivo have remained unclear. Aim: To investigate the effect of 3-BrPA to human gastric cancer implant tumors in nude mice. Materials and Methods: Animals were randomly divided into 6 groups: 3-BrPA low, medium and high dose groups, PBS negative control group 1 (PH7.4), control group 2 (PH 6.8-7.8) and positive control group receiving 5-FU. The TUNEL method was used to detect apoptosis, and cell morphology and structural changes of tumor tissue were observed under transmission electron microscopy (TEM). Results: 3-BrPA low, medium, high dose group, and 5-FU group, the tumor volume inhibition rates were 34.5%, 40.2%, 45.1%, 47.3%, tumor volume of experimental group compared with 2 PBS groups (p<0.05), with no significant difference between the high dose and 5-FU groups (p>0.05). TEM showed typical characteristics of apoptosis. TUNEL demonstrated apoptosis indices of 28.7%, 39.7%, 48.7% for the 3-BrPA low, medium, high dose groups, 42.2% for the 5-FU group and 5% and 4.3% for the PBS1 (PH7.4) and PBS2 (PH6.8-7.8) groups. Compared each experimental group with 2 negative control groups, there was significant difference (p<0.05); there was no significant difference between 5-FU group and medium dose group (p>0.05), but there was between the 5-FU and high dose groups (p<0.05). Conclusions: This study indicated that 3-BrPA in vivo has strong inhibitory effects on human gastric cancer implant tumors in nude mice.

• 한국식품과학회지
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• 제48권5호
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• pp.496-501
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• 2016

본 연구는 농축 블루베리주스를 제조 시 구연산(CA) 또는 산성메타인산나트륨(ASM)을 산미료로써 이용하여 열처리과정에서 블루베리의 폴리페놀과 안토사이아닌과 같은 기능성 성분농도와 산화방지활성, elastase 억제활성 및 tyrosinase 억제활성 등의 저하를 최소화시키는데 있다. 열처리한 농축 블루베리주스의 색가는 1% CA+1% ASM 처리군이 54.672로 가장 높았고, 1% CA 처리군이 33.034, 대조군이 22.083의 순으로 나타났다. 총 안토사이아닌 함량에서도 1% CA+1% ASM 처리군이 65.747 mg/100 g, 1% CA 처리군, 대조군 순으로 각각 46.022, 30.864 mg/100 g으로 나타났다. 산화방지활성으로써 DPPH radical 소거능의 경우 대조군은 67.983%, 1% CA 처리군은 75.486%, 1% CA+1% ASM 처리군은 87.338%로 나타났다. Elastase 억제활성에서는 대조군, 1% CA, 1% CA+1% ASM 처리군의 결과 값은 각각 26.063, 26.336, 28.285%로 나타났다. Tyrosinase 억제활성에서는 대조군 26.375%, 1% CA 처리군 67.598%, 1% CA+1% ASM 처리군은 77.891% 순으로 높게 나타났다. 모든 분석 치에서 대조군과 1% CA+1% ASM 처리군 간에는 5% 내에서 유의성 차이가 있었다. 본 결과를 통해 ASM처리가 열처리과정에서 기능성성분과 생리활성의 저하를 억제하여주는 역할을 하는 것으로 나타났으며 새로운 산미료로서 활용할 수 있을 것으로 기대한다.