The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Resistance Activity of Kyung-Ok-Ko on Thermal Stress in C. elegans

경옥고(瓊玉膏)의 열 스트레스에 의한 피부노화 억제 활성

  • Won-Seok Jung (National Institute for Korean Medicine Development ) ;
  • Sung-Young Cho (National Institute for Korean Medicine Development ) ;
  • Hyun-Woo Cho (National Institute for Korean Medicine Development ) ;
  • Hee-Woon Lee (Institute of Herbal Medicine Resources, Dongwoodang pharmacy Co. ) ;
  • Young‐IL Jeong (Dept. of Dental Materials, College of Dentistry, Chosun University) ;
  • Hee-Taek Kim (Dept. of Korean Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University ) ;
  • Young-Bob Yu (Dept. of Paramedicine, Nambu University)
  • Received : 2024.01.12
  • Accepted : 2024.01.31
  • Published : 2024.02.25
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Abstract

Objectives : This study was conducted to reveal the scientific mechanism of the anti-skin aging activity of Kyung-Ok-Ko(KOK), which is highly useful as a Korean traditional medicine and functional food. Methods : The skin wrinkle and aging inhibitory activity of KOK was confirmed through in vitro experiments of human dermal fibroblast neonatal cell(HDFn) and in vivo of C. elegans, and hairless mouse(SKH-1). Results : The amount of the C-terminus of the collagen precursor in the HDFn cell culture medium treated with KOK using an enzymes-linked immunoassay kit. The group treated with KOK 200㎍/㎖ was a 28.3% increase of collagen precursor compared to the control group. KOK showed inhibitory activity of MMP-1 compared to the control group at a concentration of 200㎍/㎖. In addition, KOK 200㎍/㎖ showed significant inhibitory activity of thermal stress and an oxidative stress compared to the control group in C. elegans. Furthermore, KOK showed a concentration-dependent(100mg/kg and 500mg/kg) anti-wrinkle formation effect in UV-irradiated hairless mouse(SKH-1). Additionally, when KOK was administered to UV-irradiated hairless mice, an increase in procollagen -1 and -3 genes expression was observed, and mmp-1 and mmp-9 genes, which increase collagen decomposition, decreased with the administration of KOK. Conclusions : The skin aging inhibition mechanism of Kyung-Ok-Ko(KOK) is presumed to be achieved through suppressing thermal stress and oxidative stress, suppressing mmp-1 and mmp-9 genes, and increasing procollagen-1 and procollagen-3.

Keywords

Acknowledgement

본 연구는 지식경제부 지역산업기술개발과제(2011)와 2021년도 남부대학교 학술연구비 지원 사업의 결과임.

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