• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.40 no.2
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• pp.29-36
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• 2008

In order to give pulp surfaces anti-bacterial functionality and photo-catalytic deodorant ability, functional pulps was made using a surface modification method with Ag nano-colloidal solution and $TiO_2$ filler. Hygiene paper was made with the specially modified pulp, and anti-bacterial and deodorant tests were carried out. The Ag nano-colloidal solution was coated on the surface of the pulp using the high pressurized gas phase squirt through the spray nozzle mounted on the hybridization system. The surface modified functional pulp was hybridized with the optimum ratio of $TiO_2$(fine particle) to pulp(core particle) under the condition of $6,000{\sim}10,000$ rpm for $3{\sim}7$ minutes in the system. The anti-bacterial functionality of the hygiene paper was confirmed by the halo test in which the formation of the clear zone around the hygiene paper sample was observed. The inhibition growth test using MIC bioscreen C showed the inhibition growth effect of the bacteria as the reaction time was increased. The photo-catalytic effect measurement of the $TiO_2$ for 4 hours of the reaction showed $50{\sim}60%$ of decomposition rate, reaching over 60% for 5 hours of the reaction.

• Korean journal of applied entomology
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• v.9 no.1
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• pp.21-24
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• 1970

Effect of seed disinfectants for controlling the soybean anthracnose was investigated with percent of seedling infection to seed or soil and with inhibition zone on potato dextrose agar. The chemicals tested were as follows: Arasan, Orthocide, Phygon-XL, P.T.A.B. and Mercuron. In seed inoculation, all the chemicals used resulted in significantly less seedling infection than that obtained in control. In the case of soil infection, the result was similar to the above but percentages of the infection were lower than the seed inoculation. Percentages of seedling infection were not necessarily correlated to the inhibition zones on potato dextrose agar by the same chemicals.

• The Korea Journal of Herbology
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• v.25 no.2
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• pp.65-70
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• 2010

Objectives : Biological activities of marine resources have been rarely evaluated compared with other herbal medicines. In the present study, we aimed to evaluate antimicrobial activities of aqueous and ethanolic extracts of five marine resources(Porphyra tenera, Laminariae Thallus, Sargassum, Ostreae Concha, Maliotidis Concha) against Propionibacterium acnes. Methods : Aqueous axtracts of five marine resources were prepared by decocting in tenfold tap water for 3 h. Etanolic extracts were obtained by extracting five marine resources with tenfold ethanol for 72 h at room temperature. The zone of growth inhibition and minimum inhibitory concentration (MIC) were determined against P. acnes after incubation for 48 h under anaerobic condition. Results : Ethanolic extracts of Porphyra tenera exhibited potent antimicrobial effects(MIC $62.5\;{\mu}g/m{\ell}$ against KCTC3320, MIC $31.25\;{\mu}g/m{\ell}$ against KCTC5527). However, all aqueous extracts tested had no effects on the growth inhibition of P. acnes. In addition, four ethanolic extracts except Porphyra tenera showed little inhibitory effect. Conclusions : These results indicate that ethanolic extracts of Porphyra tenera has antimicrobial activities against P. acnes and also warrant further development of Porphyra tenera extracts as a natural anti-acne agent.

• Preventive Nutrition and Food Science
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• v.10 no.2
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• pp.130-133
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• 2005

The growth inhibitiory effect of chaff vinegar against various food-borne pathogenic bacteria was evaluated. Bacterial growth was evaluated in chaff vinegar at concentrations of 15, 30, 50, 65, 80, and $100\%$ using the paper disc diffusion method and 0.5, 1.0, 1.5, 1.8, 2.0, 2.2 and $2.5\%$ in broth. In the paper disc diffusion assay, chaff vinegar showed a clear zone on both the Gram-positive bacteria; Listeria monocytogenes, Staphylococcus aureus and Gram-negative bacteria; Escherichia coli O157:H7, Salmonella Typhimurium, and Vibrio parahaemolyticus. Chaff vinegar exhibited the greatest growth inhibition for V. parahaemolyticus. The bactericidal effect of chaff vinegar on the E. coli O157:H7 was tested at concentrations ranging from 0.5 to $2.5\%$ (v/v) in the LB broth media. Chaff vinegar retarded the lag phase time of the growth curve in proportion in a concentration-dependent manner. Chaff vinegar at $2.5\%$ completely inhibited the growth of E. coli O157:H7.

• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.874-879
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• 2008

Tyrosinase is a key enzyme for melanin biosynthesis, and hyperpigmentation disorders are associated with abnormal accumulation of melanin pigments, which can be reduced by treatment with depigmenting agents. The methanol extract of Lespedeza cyrtobotrya $M_{IQ}$ showed inhibitory activity against mushroom tyrosinase. The active compound was purified from the methanol extract of L. cyrtobotrya, followed by several chromatographic methods, and identified as dalbergioidin (DBG) by spectroscopic methods. The results showed that DBG exhibited tyrosinase inhibitory activity with an $IC_{50}$ of $20\;{\mu}M$. The kinetic analysis of tyrosinase inhibition revealed that DBG acted as a noncompetitive inhibitor. In addition, DBG showed a melanin biosynthesis inhibition zone in the culture plate of Streptomyces bikiniensis that has commonly been used as an indicator organism. Furthermore, $27\;{\mu}M$ DBG decreased more than 50% of melanin contents on the pigmentation using the immortalized mouse melanocyte, melan-a cell.

• Microbiology and Biotechnology Letters
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• v.49 no.1
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• pp.18-23
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• 2021

The aim of this study was to determine the production of folate, short chain fatty acids (SCFAs), and antimicrobial activity exhibited by Lactobacillus plantarum G72 for potential dietary application in pregnant women. L. plantarum G72 has been reported to possess characteristic activities and functionality including β-galactosidase activity and antioxidant activities. L. plantarum G72 showed antibacterial activity against pathogenic bacteria (Listeria monocytogenes ATCC 15313, Salmonella typhimurium P99, Escherichia coli ATCC 25922, and Staphylococcus aureus KCCM 11335) using a modified method, and formation of the largest inhibition zone was observed against S. aureus KCCM 11335 (12.0-17.0 mm). The adherence of four food-borne pathogenic bacteria to HT-29 cells was inhibited by L. plantarum G72 (0.13 to 0.92 log CFU/ml). The most considerable inhibition of adherence to HT-29 cells was observed by using L. plantarum G72 against S. typhimurim P99. Additionally, folate production by L. plantarum G72 was 50.1 ng/ml, and L. plantarum G72 produced relatively more lactic acid (11,176.73 mg/kg) than acetic, propionic, or butyric acids. Therefore, the results of this study suggest that L. plantarum G72 may serve as a multifunctional food additive in the health industry.

• Korean Journal of Veterinary Service
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• v.15 no.2
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• pp.93-100
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• 1992

The purpose of the present survey was to evaluate the antibiotic residues in meats and internal organs such as muscle, liver, heart, kidney and spleen of cattle (n=59) and pigs (n=115). The EEC-4-plate-method were employed. The results were obtained as follows ; 1. In BS 6.0, BS 7.2 and BS 8.0 used as media to detect antibiotic residues, the zone($M{\pm}SD,$ cm) of bacterial growth inhibition was narrow($1.40{\pm}0$) in meats, whereas the zone was wide($1.69{\pm}0.25-1.88{\pm}0.23$ and $1.58{\pm}0.18-1.86{\pm}0.15$ in cattle and pigs, respectively) in internal organs. But in SL 8.0, it was difficult to detect the zones ($0-1.40{\pm}0$) of both meats and internal organs. 2. Residues of antibiotic in beef and pork were rarely detected in BS 6.0, BS 7.2 and BS 8.0 (range 1.7-11.9% and 2.6-4.3%, respectively), whereas residual percentages of internal organs were relatively higher(range 69.5-96.6% and 43.5-84.3%, respectively). But in SL 8.0, it was not detected in both beef or pork, whereas they were 0-13.6% and 0-4.3% in interanal organs.

• The Korean Journal of Community Living Science
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• v.24 no.1
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• pp.27-36
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• 2013

This study was performed to investigate the antimicrobial effect against food-borne pathogens of Sanguisorbae officinalis L. ethanol extract. The antimicrobial activity of the ethanol extract was determined using a paper disc-diffusion method and the diameter of the clear zone was measured. The diameters of the clear zone in the presence of 10 mg of the ethanol extract were the maximum against Staphylococcus aureus among the tested 4 gram-positive bacteria and Pseudomonas aeruginosa among the tested 7 gram-negative bacteria. Analysis of the minimum inhibition concentrations (MIC) showed that the ethanol extract exhibited a similar efficacy as sorbic acid, well-known chemical preservatives. The growth inhibitory effects of the ethanol extract in the concentrations of 250, 500, 1,000 and 2,000 mg/L on food-borne pathogens were determined against Staphylococcus aureus, Bacillus subtilis, Salmonella Typhimurium and Pseudomonas aeruginosa. The growth of the microorganisms was significantly (p<0.05) inhibited by the ethanol extract in the concentrations higher than 250 mg/L. Thus, the results of the present study demonstrate that the ethanol extract exhibits antimicrobial effects against food-borne pathogens, suggesting that Sanguisorbae officinalis L. could be used as natural antibacterial agent in food.

• Journal of fish pathology
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• v.37 no.1
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• pp.37-48
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• 2024

This study evaluated in vitro antimicrobial properties of Bacillus subtilis KCTC 1326 as an environmentally friendly alternative to antibiotics. B. subtilis KCTC 1326 was characterized on biochemical properties and antibiotics susceptibility. It exhibited antimicrobial effects against all 12 species of fish bacteria used in this experiment. Among them, the largest antibacterial zone was observed for Streptococcus parauberis (34 mm), while the smallest antibacterial zone was observed for Citrobacter freundii (8 mm). Additionally, in the co-culture inhibitory assay of B. subtilis and Edwardsiella piscicida, the growth of E. piscicida was suppressed with increasing concentrations of B. subtilis KCTC 1326, with complete inhibition observed at 107 and 108 CFU/mL of B. subtilis KCTC 1326 after 24 hours of incubation. Moreover, at 48 hours of incubation, the growth of E. piscicida was inhibited across all concentration ranges of B. subtilis KCTC 1326. Therefore, this study indicated the utilizing of B. subtilis KCTC 1326 as an antimicrobial for controlling fish bacterial diseases.

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.783-789
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• 2005

Vibriocidal GOCHUJANG was manufactured using extract of natural products and green tea. Antioxidative capacity of natural products and combination of natural products was measured with Oxidation-Reduction Potential (ORP) system value. Inhibitory ability of vibriocidal GOCHUJANG was compared with Inhibition Zone Diameter (IZD) values. Phellodendri cortex, Schizandrae fructus, Theae folium, Scutellaria baicalensis george and Acanthodanacis cortex and combination of these natural products were showed higher antioxidant capacity and inhibitory effect against V. para-haemolyticus. Two kinds of combinations, A combination was composed of Phellodendri cortex, Schizandrae fructus, Theae folium, Scutellaria baicalensis george, and B combination was consisted of Acanthodanacis cortex and A combination, were presented higher inhibition ratio with IZD values as $1.57\pm0.0051$ and $1.56\pm0.0071$, respectively. Vibriocidal GOCHUJANG was prepared based on A and B combination with their higher IZD Value as $1.76\pm0.0103$ and $1.79\pm0.0103$, respectively. When vinegar was added to A and B based GOCHUJANG, their IZD value was more higher as $2.15\pm0.0071$ and $2.44\pm0.0086$, respectively B combination based GOCHUJANG was exhibited higher inhibitory effect than A based GOCHUJANG. Combination of natural products was more important process for increasing vibriocidal effect. But addition of soybean malt or chitosan or a garlic into A or B combined GOCHUJANG, no different effect was exhibited.