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Inhibitory Effect of Dalbergioidin Isolated from the Trunk of Lespedeza cyrtobotrya on Melanin Biosynthesis  

Baek, Seung-Hwa (Korea Research Institute of Bioscience and Biotechnology)
Kim, Jin-Hee (Korea Research Institute of Bioscience and Biotechnology)
Kim, Dong-Hyun (Korea Research Institute of Bioscience and Biotechnology)
Lee, Chan-Yong (Department of Microbiology and Biotechnology, Daejeon University)
Kim, Ji-Young (Division of Bioscience and Biotechnology, Konkuk University)
Chung, Dae-Kyun (Skin Biotechnology Center, Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University)
Lee, Choong-Hwan (Division of Bioscience and Biotechnology, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.5, 2008 , pp. 874-879 More about this Journal
Abstract
Tyrosinase is a key enzyme for melanin biosynthesis, and hyperpigmentation disorders are associated with abnormal accumulation of melanin pigments, which can be reduced by treatment with depigmenting agents. The methanol extract of Lespedeza cyrtobotrya $M_{IQ}$ showed inhibitory activity against mushroom tyrosinase. The active compound was purified from the methanol extract of L. cyrtobotrya, followed by several chromatographic methods, and identified as dalbergioidin (DBG) by spectroscopic methods. The results showed that DBG exhibited tyrosinase inhibitory activity with an $IC_{50}$ of $20\;{\mu}M$. The kinetic analysis of tyrosinase inhibition revealed that DBG acted as a noncompetitive inhibitor. In addition, DBG showed a melanin biosynthesis inhibition zone in the culture plate of Streptomyces bikiniensis that has commonly been used as an indicator organism. Furthermore, $27\;{\mu}M$ DBG decreased more than 50% of melanin contents on the pigmentation using the immortalized mouse melanocyte, melan-a cell.
Keywords
Lespedeza cyrtobotrya; dalbergioidin; tyrosinase; melan-a cell; melanin biosynthesis inhibitor; skin-whitening;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
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