• Animal Bioscience
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• 제34권11호
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• pp.1757-1765
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• 2021

Objective: The swine leukocyte antigen (SLA) gene group, which is closely linked and highly polymorphic, has important biomedical significance in the protection and utilization of germplasm resources. However, genetic polymorphism analyses of SLA microsatellite markers in Chinese miniature pigs are limited. Methods: Eighteen pairs of microsatellite primers were used to amplify the SLA regions of seven miniature pig breeds and three wild boar breeds (n = 346) from different regions of China. The indexes of genetic polymorphism, including expected heterozygosity (He), polymorphic information content (PIC), and haplotype, were analyzed. The genetic differentiation coefficient (Fst) and neighbor-joining methods were used for cluster analysis of the breeds. Results: In miniature pigs, the SLA I region had the highest numbers of polymorphisms, followed by the SLA II and SLA III regions; the region near the centromere had the lowest number of polymorphisms. Among the seven miniature pig breeds, Diannan small-ear pigs had the highest genetic diversity (PIC value = 0.6396), whereas the genetic diversity of the Hebao pig was the lowest (PIC value = 0.4330). The Fst values in the Mingguang small-ear, Diannan small-ear, and Yunnan wild boars were less than 0.05. According to phylogenetic cluster analysis, the South-China-type miniature pigs clustered into one group, among which Mingguang small-ear pigs clustered with Diannan small-ear pigs. Haplotype analysis revealed that the SLA I, II, and III regions could be constructed into 13, 7, and 11 common haplotypes, respectively. Conclusion: This study validates the high genetic diversity of the Chinese miniature pig. Mingguang small-ear pigs have close kinship with Diannan small-ear pigs, implying that they may have similar genetic backgrounds and originate from the same population. This study also provides a foundation for genetic breeding, genetic resource protection, and classification of Chinese miniature pigs.

• Asian-Australasian Journal of Animal Sciences
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• 제32권11호
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• pp.1664-1672
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• 2019

Objective: This study aimed to measure genetic diversity and to determine the relationships among fourteen goose breeds. Methods: Microsatellite markers were isolated from the genomic DNA of geese based on previous literature. The DNA segments, including short tandem repeats, were tested for their diversity among fourteen populations of geese. The diversity was tested on both breeds and loci level and by mean of unweighted pair group method with arithmetic mean and structure program, phylogenetic tree and population structure were tested. Results: A total of 108 distinct alleles (1%) were observed across the fourteen breeds, with 36 out of the 108 alleles (33.2%) being unique to only one breed. Genetic parameters were measured per the 14 breeds and the 9 loci. Medium to high heterozygosity was reported with high effective numbers of alleles (Ne). Polymorphic information contents (PIC) of the screened loci was found to be highly polymorphic for eleven breeds; while 3 breeds were reported moderately polymorphic. Breeding coefficient ($F_{IS}$) ranged from -0.033 to 0.358, and the pair wise genetic differentiation ($F_{ST}$) ranged from 0.01 to 0.36 across the fourteen breeds; for the 9 loci observed and expected heterozygosity, and Ne were same as the breeds parameters, PIC of the screened loci reported 6 loci highly polymorphic and 3 loci to be medium polymorphic, and $F_{IS}$ ranged from -0.113 to 0.368. In addition, genetic distance estimate revealed a close genetic distance between Canada goose and Hortobagy goose breeds by 0.04, and the highest distance was between Taihu goose and Graylag goose (anser anser) breed by 0.54. Conclusion: Cluster analyses were made, and they revealed that goose breeds had hybridized frequently, resulting in a loss of genetic distinctiveness for some breeds.

• 융합정보논문지
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• 제11권6호
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• pp.107-112
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• 2021

본 연구는 제주지역 카페 중 제주의 고유한 특성을 반영하여 건축한 카페를 연구대상으로 분석하였다. 이는 제주의 특성을 반영한 카페가 차별성과 고유성을 확보하여 나름의 경쟁력과 관광객의 지속적인 관심을 유지하고 있다는 점에서 제주에서 카페를 창업하고자하는 예비창업자들에게 실질적인 도움을 주고자 함이다. 또한 카페 건축에 적용된 제주 고유의 특성들을 살펴봄으로써 제주지역을 이해하는 학술적 기반과 적용가능한 제주의 특성을 정리하였다. 연구는 문헌분석과 현장답사를 병행하는 방법으로 진행하였으며, 그 결과 카페에서 적용된 제주지역의 특성으로서 다음과 같은 내용을 알 수 있었다. 첫째, 현상적 물성의 반영이다. 이는 제주의 현무암 등 독특한 건축재료, 형태, 지붕 등 기존 건물의 물리적 요소를 유지하는 것으로 나타났다. 둘째, 제주의 해석적 일상의 반영이다. 이는 제주 옛집의 배치, 일상소품 등을 카페에 유지하는 것으로 나타났다. 셋째, 제주의 서사적 시간성의 반영이다. 이는 기존 건물이 가진 역사적 사건과 이야기, 시간 등이 누적된 모습을 유지하는 것으로 나타났다. 이러한 점에서 제주에서 제주의 특성을 반영한 카페를 창업할 경우, 위의 세 가지 특성을 융합하여 구축한다면 매우 특성 있는 카페를 구현할 수 있을 것이다.

• Journal of Plant Biotechnology
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• 제48권1호
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• pp.26-33
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• 2021

당귀는 일반적으로 이용되는 대표적인 다년생의 약용식물이다. 최근 국제적 추세에 따라 자국의 유전자원의 발굴, 보존 등이 강화됨에 따라 인접국가와 국내 자생 당귀 계통을 판별할 수 있는 기준설정에 관한 연구의 필요성이 대두되고 있지만, 분자생물학적 판별 기술의 개발은 아직 미흡한 실정이다. 본 연구에서는 국내 토종 당귀인 참당귀와 세발당귀, 그리고 해외 유래 당귀 종의 기원을 판별하기 위해 핵의 리보솜에 존재하는 ITS 유전자단편에서 SNP를 이용한 판별 프라이머를 확보하였으며, 이를 보완하여 보다 신속하게 판별하기 위하여 ARMS-PCR 기술을 이용한 판별 마커와 그 조건을 확립하였다. 그러므로, 본 연구에서 개발된 SNP 마커는 다양한 지역 또는 국가에서 서식하는 당귀 종들의 신속한 확인을 위해 매우 유용하게 이용될 것으로 생각된다.

• 보건행정학회지
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• 제31권1호
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• pp.35-45
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• 2021

Background: As a descriptive study targeting 2,068 cancer patients as men of national merit in 2013, this study aims to provide the basic data for systematizing the early diagnosis and treatment of cancer by comparatively analyzing the 5-year survival rate. Methods: This study researched the survival of cancer patients through Electronic Medical Record and Patriots-Veterans Qualification Program, targeting 2,068 newly-diagnosed cancer patients verified in five veterans hospitals and consigned management system. This study verified differences between general characteristics of cancer patients as men of national merit and analyzed their survival rate. Results: The cancer patients as men of national merit were super-aged as their average age was 72.5. In the analysis of general characteristics of five major prevalent cancers, there were statistically significant differences according to age, region, cancer diagnostic path, differentiation, diagnostic method, treatment method, SEER stage, and survival period, except for the types of the man of national merit (p<0.001). The whole survival rate of cancer patients as men of national merit was 50%. The 5-year survival rates of predisposing cancers were shown as prostate cancer (79%), colorectal cancer (64%), gastric cancer (57%), liver cancer (32%), and lung cancer (12%). In the cancer diagnostic path, all the predisposing cancers showed the highest survival rate in medical examination. In the treatment method, the surgery showed the highest survival rate. The cancer patients as men of national merit showed a lower survival rate than the general cancer patients of Korea. Conclusion: It would be needed to guarantee the honorable and happy life through health recovery as special treatment of contribution and sacrifice of super-aged men of national merit by increasing the cancer survival rates through regular checkup, early diagnosis, and high-quality treatment system that could have important effects on the survival rate according to the occurrence of cancers.

• 사물인터넷융복합논문지
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• 제7권4호
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• pp.1-7
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• 2021

현재 스마트 가전의 새로운 시장수요를 만족하기 위해 IoT 기술을 이용한 제품의 기술 차별화(센서, AI 등)가 많은 호응을 얻고 있다. 그러나 에어컨 제품은 융합기술의 초기 단계에 있다. 따라서 에어컨 제품은 IoT를 넘어 정보 생산, 수집, 처리, 저장 및 서비스 개발의 ICT 기술이 필요한 분야이다. 우리가 제안하는 기술은 IR-UWB를 이용한 비접촉방식의 생체신호를 수집 및 저장한다. 생체신호에 따라 에어컨의 방향을 제어하고 사용자의 수면을 모니터링하여 최적의 숙면 환경을 제공한다. 그리고 불쾌지수 환경에 따라 에어컨의 최적조건과 감성조명의 변화로 쾌적함과 안락함을 제공할 수 있는 서비스 알고리즘을 제안한다. 본 연구를 통하여 생체신호, 불쾌지수 및 감성조명의 ICT 기술을 에어컨 서비스를 사용자들이 이용할 수 있도록 알고리즘을 연구하였다.

• Earthquakes and Structures
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• 제24권1호
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• pp.37-51
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• 2023

Türkiye has made significant changes and updates in both seismic risk maps and design codes over time, as have other countries with high seismic risk. In this study, the last two seismic design codes and risk maps were compared for the Aegean Region (Western Türkiye) where the earthquake risk has once again emerged with the 2020 Izmir Earthquake (Mw=6.9). In this study, information about the seismicity of the Aegean Region was given. The seismic parameters for all provinces in the region were compared with the last two earthquake risk maps. The spectral acceleration coefficients of all provinces have increased and differentiated with the current seismic hazard map as a result of the design spectra used on a regional basis have been replaced by the geographical location-specific design spectra. In addition, section damage limits were obtained for all provinces within the scope of the last two seismic design codes. Structural analyses for a sample reinforced-concrete building were made separately for each province using pushover analysis. The deformations in the cross-sections were compared with the limit states corresponding to the damage levels specified in the last two seismic design codes for the region. Target displacement requests for all provinces have decreased with the current code. The differentiation of geographical location-specific design spectra both in the last two seismic design code and between provinces has caused changes in section damages and building performance levels. The main aim of this study is to obtain and compare both seismic and structural analysis results for all provinces in the Aegean Region (Western Türkiye).

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.219-219
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• 2022

Granule-bound starch synthase I (GBSSI), encoded by the waxy gene, is responsible for the accumulation of amylose during the development of starch granules in rice endosperm. Despite many findings on waxy alleles, the genetic diversity and evolutionary studies are still not fully explored regarding their functional effects. Comprehensive evolutionary analyses were performed to investigate the genetic variations and relatedness of the GBSSI gene in 374 rice accessions composed of 54 wild accessions and 320 bred cultivars (temperate japonica, tropical japonica, indica, aus, aromatic, and admixture). GBSS1 coding regions were analyzed from a VCF file retrieved from whole-genome resequencing data, and eight haplotypes were identified in the GBSSI coding region of 320 bred cultivars. The genetic diversity indices revealed the most negative Tajima's D value in the tropical-japonica, followed by the aus and temperate-japonica, while Tajima's D values in indica were positive, indicating balancing selection. Diversity reduction was noticed in temperate japonica (0.0003) compared to the highest one (wild, 0.0044), illustrating their higher genetic differentiation by F_ST-value (0.604). The most positive Tajima's D value was observed in indica (0.5224), indicating the GBSSI gene domestication signature under balancing selection. In contrast, the lowest and negative Tajima's D value was found in tropical japonica (-0.5291), which might have experienced a positive selection and purified due to the excess of rare alleles. Overall, our study offers insights into haplotype diversity and evolutionary fingerprints of GBSSI. It ako provides genomic information to increase the starch content of cooked rice.

• 대한영상의학회지
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• 제83권1호
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• pp.70-83
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• 2022

급성 관동맥 증후군(acute coronary syndrome)은 대부분 죽상경화 관상동맥 질환(atherosclerotic coronary artery disease)에 의해 발생하지만, 비죽상경화 관상동맥 질환에서도 발생할 수 있다. 고식적 관상동맥 혈관조영술은 동맥 내강의 협착이나 확장 등의 형상에 대한 정보만을 제공하고, 동맥경화반이나 동맥벽에 대한 평가가 어려워 관상동맥 이상의 원인 질환의 진단에 낮은 특이도를 보인다. 반면, 관상동맥 전산화단층촬영 혈관조영술은 혈관경화반의 특징, 혈관벽의 조영증강뿐 아니라 연접한 대동맥이나 폐동맥의 변화 등도 함께 관찰할 수 있어, 비죽상경화 관상상동맥질환의 진단 및 다양한 원인 감별에 도움이 된다. 따라서 이종설에서는 다양한 비죽상경화 관상동맥 질환들을 소개하고, 이의 병태생리 및 대표적인 관상동맥 전산화단층촬영 혈관조영술의 영상 소견에 대해 설명하고자 한다.

• Korean Journal of Radiology
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• 제24권6호
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• pp.574-589
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• 2023

Radiopharmaceuticals targeting prostate-specific membrane antigens (PSMA) are essential for the diagnosis, evaluation, and treatment of prostate cancer (PCa), particularly metastatic castration-resistant PCa, for which conventional treatment is ineffective. These molecular probes include [⁶⁸Ga]PSMA, [¹⁸F]PSMA, [Al¹⁸F]PSMA, [^99mTc]PSMA, and [⁸⁹Zr]PSMA, which are widely used for diagnosis, and [¹⁷⁷Lu]PSMA and [²²⁵Ac]PSMA, which are used for treatment. There are also new types of radiopharmaceuticals. Due to the differentiation and heterogeneity of tumor cells, a subtype of PCa with an extremely poor prognosis, referred to as neuroendocrine prostate cancer (NEPC), has emerged, and its diagnosis and treatment present great challenges. To improve the detection rate of NEPC and prolong patient survival, many researchers have investigated the use of relevant radiopharmaceuticals as targeted molecular probes for the detection and treatment of NEPC lesions, including DOTA-TOC and DOTA-TATE for somatostatin receptors, 4A06 for CUB domain-containing protein 1, and FDG. This review focused on the specific molecular targets and various radionuclides that have been developed for PCa in recent years, including those mentioned above and several others, and aimed to provide valuable up-to-date information and research ideas for future studies.