• Structural Engineering and Mechanics
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• 제84권2호
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• pp.225-238
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• 2022

In order to improve the cracking resistance of reinforced concrete and give full play to the advantages of prefabricated assembly structure in construction, prestressed reinforced concrete composite beam (PRCC) is proposed. Through the bending static test of seven I-shaped beam specimens, the bending failure modes and bearing capacity of PRCC and reinforced concrete composite beam are compared and analyzed, and the effects of prestress size, prestressed reinforcement layout and prestress application sequence on the flexural behavior of PRCC beams are studied. The results show that the cracking load and ultimate load of PRCC beams significantly increased after prestressing, and prestressed tendons can effectively control the crack development. With the increase of prestressing degree, the deformation resistance and bending stiffness of PRCC beams are increased. The application sequence of prestress has little influence on the mechanical properties of PRCC beams. The crack width, stiffness and normal section bearing capacity of PRCC beam are analyzed, and the calculated results are in good agreement with the experimental results.

• Molecular Medicine Reports
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• 제20권4호
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• pp.3301-3307
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• 2019

c-Myc is a characteristic oncogene with dual functions in cell proliferation and apoptosis. Since the overexpression of the c-Myc proto-oncogene is a common event in the development and growth of various human types of cancer, the present study investigated whether oncogenic c-Myc can alter natural killer (NK) cell-mediated immunity through the expression of associated genes, using PCR, western blotting and flow cytometry assays. Furthermore, whether c-Myc could influence the expression levels of natural killer group 2 member D (NKG2D) ligands, which are well known NK activation molecules, as well as NK cell-mediated immunity, was investigated. c-Myc was inhibited by 10058-F4 treatment and small interfering RNA transfection. Upregulation of c-Myc was achieved by transfection with a pCMV6-myc vector. The inhibition of c-Myc increased MHC class I polyeptide-related sequence B and UL16 binding protein 1 expressions among NKG2D ligands, and the overexpression of c-Myc suppressed the expression of all NKG2D ligands, except MHC class I polyeptide-related sequence A. Furthermore, the alteration of c-Myc activity altered the susceptibility of K562 cells to NK cells. These results suggested that the overexpression of c-Myc may contribute to the immune escape of cancer cells and cell proliferation. Combined treatment with NK-based cancer immunotherapy and inhibition of c-Myc may achieve improved therapeutic results.

• Molecules and Cells
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• 제27권5호
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• pp.539-546
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• 2009

In Saccharomyces cerevisiae, ribosomal protein L7, one of the ~46 ribosomal proteins of the 60S subunit, is encoded by paralogous RPL7A and RPL7B genes. The amino acid sequence identity between RPl7a and RPl7b is 97 percent; they differ by only 5 amino acid residues. Interestingly, despite the high sequence homology, Rpl7b is detected in both the cytoplasm and the nucleolus, whereas Rpl7a is detected exclusively in the cytoplasm. A site-directed mutagenesis experiment revealed that the change in the amino acid sequence of Rpl7b does not influence its subcellular localization. In addition, introns of RPL7A and RPL7B did not affect the subcellular localization of Rpl7a and Rpl7b. Remarkably, Rpl7b was detected exclusively in the cytoplasm in rpl7a knockout mutant, and overexpression of Rpl7a resulted in its accumulation in the nucleolus, indicating that the subcellular localization of Rpl7a and Rpl7b is influenced by the intracellular level of Rpl7a. Rpl7b showed a wide range of localization patterns, from exclusively cytoplasmic to exclusively nucleolar, in knockout mutants for some rRNA-processing factors, nuclear pore proteins, and large ribosomal subunit assembly factors. Rpl7a, however, was detected exclusively in the cytoplasm in these mutants. Taken together, these results suggest that although Rpl7a and Rpl7b are paralogous and functionally replaceable with each other, their precise physiological roles may not be identical.

• 정보처리학회논문지B
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• 제16B권1호
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• pp.17-24
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• 2009

본 논문에서는 교차로 내 사고를 감지하기 위하여 배경영상과 교차로 내에 설치된 사거리 신호등의 주기를 이용한 교차로 사고감지 알고리즘을 제안한다. 기존의 영상을 이용한 방법에서는 새로운 사고모델이나 혼잡한 상황, 음원을 이용할 경우에 소음이 크게 발생하는 상황에서 사고 검지율이 낮아지는 문제점을 내포한다. 본 논문에서는 차량 및 외부 그림자 또는 차량의 조명등의 영향으로 인한 사고 오 판단을 줄이기 위하여 신호등의 주기와 배경영상의 히스토그램의 속성을 이용한 필터를 개발하여 사고감지에 이용하였다. 제안된 알고리즘의 성능을 알아보기 위하여 15개의 실제 사고영상을 획득하여 실험한 결과 15개의 동영상에서 모두 사고를 감지하였으며, 새로운 사고 모델에 대해서도 교차로 내사고를 감지 할 수 있었다.

• Smart Structures and Systems
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• 제31권2호
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• pp.113-130
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• 2023

Hybrid simulation (HS) has attracted community attention in recent years as an efficient and effective experimental technique for structural performance evaluation in size-limited laboratories. Traditional hybrid simulations usually take deterministic properties for their numerical substructures therefore could not account for inherent uncertainties within the engineering structures to provide probabilistic performance assessment. Reliable structural performance evaluation, therefore, calls for stochastic hybrid simulation (SHS) to explicitly account for substructure uncertainties. The experimental design of SHS is explored in this study to account for uncertainties within analytical substructures. Both computational simulation and laboratory experiments are conducted to evaluate the pseudo-random Sobol sequence for the experimental design of SHS. Meta-modeling through polynomial chaos expansion (PCE) is established from a computational simulation of a nonlinear single-degree-of-freedom (SDOF) structure to evaluate the influence of nonlinear behavior and ground motions uncertainties. A series of hybrid simulations are further conducted in the laboratory to validate the findings from computational analysis. It is shown that the Sobol sequence provides a good starting point for the experimental design of stochastic hybrid simulation. However, nonlinear structural behavior involving stiffness and strength degradation could significantly increase the number of hybrid simulations to acquire accurate statistical estimation for the structural response of interests. Compared with the statistical moments calculated directly from hybrid simulations in the laboratory, the meta-model through PCE gives more accurate estimation, therefore, providing a more effective way for uncertainty quantification.

• 한국실내디자인학회논문집
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• 제23권1호
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• pp.61-68
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• 2014

Recently, there have been increasing attempts to pursue and express feelings such as sensibilities, emotions, and impressiveness in commercial spaces. One of such methods is to apply 'storytelling' to space designs. Applicability of storytelling to a space suggests that the contents of a space can be expressed through various mediums. Portraying events and situations through a single time continuity of a story is referred to as 'narrative'. The movement of users and sequence of contents are determined by a narrative. It provides different storytelling and a sense of place to each space through various roles, such as wide association, engraving, and image formation. A narrative can lead users to engage in different perceptions and behaviors even in spaces with the same content. Thus, this study is intended to examine the impact of space marketing in line with design narratives, assuming that narratives of commercial space designs will influence the formation of brand identity. The research methods are as follows. First, the definition of narratives in space design was established by examining narrative architectures. Second, design analysis tools for commercial spaces were established from the perspective of narratives through preceding studies. Third, the design narratives of different shops under the same brand were comparatively analyzed through a case study. To carry out a case study, a commercial space of 'O'sulloc' was selected, and its brand identity was studied from the narrative standpoint. The case study involved interior designs of 7 road shops of 'O'sulloc.' Among the 7 road shops, two of them with the biggest difference in design narratives were selected, and an observation survey was done on the users as a second analysis. Through the observation survey, actual design narrative experience was analyzed in 4 steps of introduction, development, turn, and conclusion. The findings are as follows. The design method of each shop varied, and different design elements were emphasized. Among various elements, the ones that reflect the brand identity of 'O'sulloc' the best were logo, product, and shape. During the process of narratives, the characteristics of each shop and user recognition and behavior varied depending on the degree of emphasis on a particular element. It suggests that space design narratives can influence the formation of brand identity. This study provides ideal directions of developing space designs necessary for forming brand identity from the standpoint of Korean traditional culture modernization. Future studies could discuss the economic feasibility of such designs.

• BMB Reports
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• 제40권3호
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• pp.315-324
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• 2007

The novel $\alpha$-amylase purified from locally isolated strain, Bacillus sp. KR-8104, (KRA) (Enzyme Microb Technol; 2005; 36: 666-671) is active in a wide range of pH. The enzyme maximum activity is at pH 4.0 and it retains 90% of activity at pH 3.5. The irreversible thermoinactivation patterns of KRA and the enzyme activity are not changed in the presence and absence of $Ca^{2+}$ and EDTA. Therefore, KRA acts as a $Ca^{2+}$-independent enzyme. Based on circular dichroism (CD) data from thermal unfolding of the enzyme recorded at 222 nm, addition of $Ca^{2+}$ and EDTA similar to its irreversible thermoinactivation, does not influence the thermal denaturation of the enzyme and its Tm. The amino acid sequence of KRA was obtained from the nucleotide sequencing of PCR products of encoding gene. The deduced amino acid sequence of the enzyme revealed a very high sequence homology to Bacillus amyloliquefaciens (BAA) (85% identity, 90% similarity) and Bacillus licheniformis $\alpha$-amylases (BLA) (81% identity, 88% similarity). To elucidate and understand these characteristics of the $\alpha$-amylase, a model of 3D structure of KRA was constructed using the crystal structure of the mutant of BLA as the platform and refined with a molecular dynamics (MD) simulation program. Interestingly enough, there is only one amino acid substitution for KRA in comparison with BLA and BAA in the region involved in the calcium-binding sites. On the other hand, there are many amino acid differences between BLA and KRA at the interface of A and B domains and around the metal triad and active site area. These alterations could have a role in stabilizing the native structure of the loop in the active site cleft and maintenance and stabilization of the putative metal triad-binding site. The amino acid differences at the active site cleft and around the catalytic residues might affect their pKa values and consequently shift its pH profile. In addition, the intrinsic fluorescence intensity of the enzyme at 350 nm does not show considerable change at pH 3.5-7.0.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2007년도 추계학술대회 논문집
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• pp.51-56
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• 2007

Pantograph of the electric train is the important part receiving current by adhereing contact wire. Wearing rate of pantograph or contact wire is changed by the type of pantograph and material of adhering part. In special, the weather influences the relative wearing rate and wearing type of pantograph Slider and Contact wire with steep wearing in arc and life cycle of pantograph slider. Presently, the weather causes side-wearing and over-wearing of pantograph slide installed in electric train. So it is difficult to manage and operate the electric train. Although the quality of slide using in line4 vehicle was finished the test in line 2 vehicle experimentally, it has to apply after pantograph is installed in some electric trains and check the influence in it and contact wire. It is because line4 section is very different from line2 section. Experimental application is the sequence to certify the safety and abrasion resistance importantly by enlarging experimental application with increase of the experimental electric train. The lenth of line4 in Seoul Metro is 71.5[km]. It is long section. The line is mixed DC 1,500[V] section and AC 25,000[V] section. It has underground section of 41.5[km] and ground section of 30[km]. Ground section is about 42% and receives much influence in the rain during the rainy season. After experimental application we found that this pantograph slide has twice as much abrasion resistance despite a little deviation and found the occurrence probability of arc and side-wearing is decreased considerably.

• KIEAE Journal
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• 제6권4호
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• pp.3-10
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• 2006

To preserve scenic-view of Gwanak Mountain from the urban development, the viewshed component ratio and the scenic-view preference are investigated and analyzed by selecting view points at Nangokro. After 16 candidate view points are selected from the site, sight component ratio is measured through visual structure analysis and preference are measured by using the Likert scale. In addition, factor analysis, and regression analysis were conducted to examine the relationship between influencing factors and preference. As a result of the regression analysis of the 9 factors to influence the scenic-view preference, two factors such as degree of natural landscape and degree of arrangement around the site are chosen to influence the scenic-view preference. Through the preference factor analysis, two preference factors are selected as follows; 1) The factor of the landscape formation - Existence and nonexistence of the scenic-view conservation, degree of the landscape opening, and degree of natural landscape, degree of the landscape originality, and degree of the landscape sequence, degree of the landscape intimacy 2) The factor surrounding the landscape - Degree of arrangement around the site, degree of the landscape complexity, and atmosphere around the site.

• 한국철도학회논문집
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• 제8권1호
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• pp.63-81
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• 2005

In this Paper, Ⅰ illustrate the continuity and severance of Korean railroad. The Korean railroad has been running for 105 years now since it started operation in 1899. Between 1905 and 1945, Japan's imperial government ruled Korea including operations of the railroad. After Korea restored its sovereignty, Korean nationals managed the Korean railroad with our own ability and strategies. At present, we can examine the effect of railroad in perspective of continuity and severance respectively. For one thing, the concept of continuity is described as influence or railroad still remains. For another, the severance shows facts and phenomena that are broken off. I have come up with some facts that are involved with continuity. For example, infrastructure and the formation of cities and land spaces are connected with the sequence. Futhermore, the railroad has made a huge dent in modernization and expedition of movement. In the course of tracing down the facts, I classified the concept of severance : the separation of South and North Korea, drastic changes in transport policy, operations and characteristics. Finally technology has brought about a major breakthrough with the development of Koran high speed rail.