• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.963-977
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• 2015

The well-characterized gram-positive bacterium Bacillus subtilis is an outstanding industrial candidate for protein expression owing to its single membrane and high capacity of secretion, simplifying the downstream processing of secretory proteins. During the last few years, there has been continuous progress in the illustration of secretion mechanisms and application of this robust host in various fields of life science, such as enzyme production, feed additives, and food and pharmaceutical industries. Here, we review the developments of Bacillus subtilis as a highly promising expression system illuminating strong chemical- and temperatureinducible and other types of promoters, strategies for ribosome-binding-site utilization, and the novel approach of signal peptide selection. Furthermore, we outline the main steps of the Sec pathway and the relevant elements as well as their interactions. In addition, we introduce the latest discoveries of Tat-related complex structures and functions and the countless applications of this full-folded protein secretion pathway. This review also lists some of the current understandings of ATP-binding cassette transporters. According to the extensive knowledge on the genetic modification strategies and molecular biology of Bacillus subtilis, we propose some suggestions and strategies for improving the yield of intended productions. We expect this to promote striking future developments in the optimization and application of this bacterium.

• Journal of the Korean Society of Industry Convergence
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• v.22 no.2
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• pp.183-189
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• 2019

Researches utilizing the fourth industrial revolution technology are being conducted as a breakthrough for improving the earthworker productivity. In order to make the earthwork site smarter, it is necessary to digitize the construction site topography at first. For this purpose, photogrammetry using drones and LiDAR on MMS have been recently used. The purpose of this study is to analyze the accuracy of LiDAR by installation angles for verifying the application of MMS in the construction site. As a result of comparing the coordinates measured by the total station and the LiDAR, a small error of about 1-2 centimeters was shown. It is confirmed that MMS could be well applied to the earthwork site. In addition, there was no significant difference in the accuracy of the acquired coordinates according to the installation angle of the LiDAR, but the shape of the point clouds was different. The larger the installation angle, the better the shape of the site terrain is measured.

• Journal of Soil and Groundwater Environment
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• v.17 no.1
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• pp.1-7
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• 2012

Selection of appropriate liner type would be the most important factor to prevent groundwater contamination by leachate from waste management site. This report introduces the IWEM (Industrial Waste Management Evaluation Model) developed by US EPA to evaluate the potential pollution of groundwater under the waste management unit and to suggest an appropriate type of liner, and provides with the results of IWEM application to a coal-ash landfill site in Korea as a case study. IWEM uses a standard method using a database, a decision-making process based on site characteristics, and the user-friendly input-and-output system. Authors evaluate this model to be applicable in Korea provided that the database is replaced into local data.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.4
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• pp.275-283
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• 2019

The purpose this study is to analyze how human resources in culture and arts education use the contents of Arts Academy training program in the field, and to suggest implications and improvements of the training for strengthening the competence of professional manpower. For this purpose, an online survey was conducted for the participants of the Arte Academy training program to investigate the actual application rate. As a result of the analysis, overall application of the training was high. However, the work-site application was divided into indirect and direct applications. Indirect application showed higher results than direct application. This shows that the training is effective in terms of strengthening the participants' personal competence, but they are somewhat lacking in applying the contents learned in the training directly in the field. Indirect application is a result of training and is a necessary part of strengthening competence. However, considering that culture and arts education is a consumer-oriented policy that enjoys culture and art, it needs to be strengthened more directly in the field, and the competence of the applicant should be applied to the field more smoothly. The Korea Arts & Culture Education Service needs to review and implement such a training that has a higher direct application than indirect application.

• Magazine of RCR
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• v.16 no.3
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• pp.39-44
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• 2021

• Journal of Industrial Technology
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• v.18
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• pp.27-34
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• 1998

According to growing interest in environmental quality with improvements of a standard of living site selection problems such as waste landfill sites, caused regional conflicts. This study investigates a waste landfill estimation method that was used to for storage, management, analysis and display of environmental information provided by geo-spatial information system(GIS) and analytic hierarchy process(AHP) as a decision-making method. If GIS is integrated with AHP, site selection problems of environmental hatred facilities shall be able to be very useful, because of AHP with flexibility which appropriately reflects opinions of the related group.

• Proceedings of the Korean Society of Computer Information Conference
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• 2023.01a
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• pp.129-130
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• 2023

본 논문에서는 키넥트 센서 기반의 산업 현장 사고 모니터링 애플리케이션을 설계하고 구현한다. 이 애플리케이션은 기존 산업 현장 사고 모니터링 인력의 의존을 줄이고, 사고 발생 자동 감지로 인한 알림 기능과 현장 기록 기능을 구현한다. 사고 발생 감지 기능은 키넥트 센서 기반으로 스켈레톤 인식 기능을 활용하여 기존에 설정된 좌표 값 이하로 헤드 조인트 좌표가 떨어질 경우 관리자에게 알림을 보내도록 구현한다. 사고감지 및 알림 시스템은 기존 모니터링 시스템을 보완하는 방식으로 감지 인력의 한계를 극복할 수 있는 기능을 제공한다.

• Journal of Korea Society of Industrial Information Systems
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• v.14 no.1
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• pp.90-94
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• 2009

In this parer, we give attention to a wireless temperature measurement system using in industrial site. Therefore, we developed and designed the remote measurement system for an industrial environment. To examine an application possibility of a industrial environment, we analyzed a temperature characteristic of wireless temperature system according to a measurement distance, and discussed about a performance difference according to an antenna change.

• Journal of Navigation and Port Research
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• v.32 no.6
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• pp.489-497
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• 2008

The government is now driving a policy that development of industrial complex site for the local manufacturing industry by reengineering and expanding the existing sites or reclamation of coastal zone. Therefore, it is necessary to analyze the impact of the coastal reclamation work in terms of physical and environmental characteristics. This study is also dealing with the impact of coastal reclamation for national industrial complex site to the ocean physical characteristics, variation of sedimentation and diffusion system and marine environment from the analysis of the field measurement and numerical simulation. The site for application is at the coastal boundary near Onsan national industrial complex, Ulsan metro city. In order to verify the numerical simulation result, it is compared to the collected data for tide, current, and sediments of the existing measurement and field observation at the selected stations for this study. The verified numerical models were applied to the actual field and the future change was analyzed.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• 2008.11a
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• pp.767-771
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• 2008

From industrial field spearhead IT and new create an added value and market with fusion of traditional industry composition researches actively to be in the process of advancing. The application possibility of high-tech IT technique is coming to be high in construction industry. Currently, the user requirements that upgrade existing system to high-tech construction project information system combined construction CALS system applying in road/river business management service with high-tech IT such as ubiquitous and mobile techniques are increasing. Therefore, the objective of this study is to verify site application of RFID and mobile equipment for applying in high-tech field with ubiquitous and mobile techniques so that construction CALS system gets advanced increasingly.