• Journal of Microbiology and Biotechnology
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• 제21권6호
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• pp.545-555
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• 2011

The diversity elucidation by amplified ribosomal DNA restriction analysis and 16S rDNA sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of Gram-positive over Gram-negative bacteria within the Kangra valley in Himachal Pradesh, India. The Gram-positive bacteria observed belong to two taxonomic groupings; Firmicutes, including the genera Bacillus and Paenibacillus; and Actinobacteria, represented by the genus Microbacterium. The Gram-negative bacteria included ${\alpha}$-Proteobacteria genera Brevundimonas, Rhizobium, and Mesorhizobium; ${\gamma}$-Proteobacteria genera Pseudomonas and Stenotrophomonas; and ${\beta}$-Proteobacteria genera Azospira, Burkholderia, Delftia, Herbaspirillum and Ralstonia. The low level of similarity of two isolates, with the type strains Paenibacillus xinjiangensis and Mesorhizobium albiziae, suggests the possibility of raising species novum. The bacterial strains of different phylogenetic groups exhibited distinct carbon-source utilization patterns and fatty acid methyl ester profiles. The strains differed in their nitrogenase activities with relatively high activity seen in the Gramnegative strains exhibiting the highest similarity to Azospira oryzae, Delftia lacustris and Herbaspirillum huttiense.

• 한국버섯학회지
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• 제18권4호
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• pp.323-330
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• 2020

본 연구에서는 유럽 양송이 자원들을 SSR marker를 통해 유전적 다양성과 집단 구조, 유전적 분화에 대하여 분석하였다. 본 연구에서 유럽의 양송이 자원들은 유전적 거리기반의 4개의 그룹으로 나뉘었고 집단구조 분석을 통하여 2개의 subpopulation으로 이루어져 있었다. 본 연구에서 사용한 SSR 마커로 유럽의 양송이 자원들은 지리적 그리고 갓색으로 구분되지 않았다. 유전적 다양성은 유전적 거리기반의 그룹에서는 Group 4, 집단구조 분석을 통한 subpopulation에서는 Pop. 2의 다양성이 높았다. 그리고 양송이 자원들은 유전적 분화가 매우 낮았다. 본 연구의 결과는 차후 양송이의 육종 등에 이용 할 수 있을 것이다.

• 한국작물학회지
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• 제45권4호
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• pp.245-250
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• 2000

This study was conducted to analyze the responses of some agronomic traits to the different cultural environments and relations among the agronomic traits for selecting sesame varieties with higher cultural stabilities. The indexes for stability parameters measured were coefficient of variability, heritabilities, genetic correlations and path coefficients of agronomic traits according to locations and years in Korea. The heritabilities of agronomic traits showed different by locations and years. Number of seeds per capsule and 1000 seeds weight showed higher heritabilities, but stem length and seed weight per plant showed relatively lower heritabilities. Average heritabilities of some agronomic traits in 1998 were comparatively higher than those of 1999. Of six areas, Jinju area showed biggest coefficient of yield variability in 1998-1999. Iksan and Taegu areas showed higher heritabilities in 1998, but Iksan and Jinju areas showed lower heritabilities in 1999. Genetic correlations were slightly higher than corresponding phenotypic correlations. Stem length showed positive genetic correlation with the number of capsules per plant, and seed weight per plant and the number of capsule per plant showed positive genetic correlation with seed weight per plant. On the analysis of path coefficients, stem length and number of capsules effected highly on grain yield. Great regional variations were observed on the effects of agronomic traits on grain yield. Higher direct effects of stem length on grain yield were observed at Suwon, Chungwon, Taegu, Jinju and Naju areas, but in Iksan area was observed higher direct effect of the number of capsules per plant on grain yield in 1998. In 1999, higher direct effect of stem length on grain yield was observed at Chungwon and Suwon areas. Iksan and Taegu areas were also observed higher direct effect of the number of capsule per plant on grain yield.

• 한국작물학회지
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• 제47권4호
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• pp.269-272
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• 2002

This study was carried out to investigate varietal differences on growth characteristics under the conditions of PE film-mulching and non-mulching in sesame. At maturing stage from 76 to 95 days after sowing, Yangbaeckkae, non-branching plant type, under non-mulching showed larger leaf area index (LAI) than that of film-mulching, while plant height and the number of capsules per plant were similar to those of film-mulching. LAI of Ahnsankkae, branching plant type, under non-mulching was similar to film-mulching, while plant height and the number of capsules per plant were smaller than those of film-mulching. Net assimilation rate (NAR) of two varieties under non-mulching was lower at seedling stage from 25 to 35 days after sowing but higher at flowering stage from 45 to 55 days after sowing. At maturing stage from 66 to 77 days after sowing, NAR and crop growth rate (CGR) of Yangbaeckkae under non-mulching were greater than those of film-mulching, whereas those of Ahnsankkae under non-mulching were lesser than those of film-mulching. Yield under non-mulching was decreased by 7 % in Yangbaeckkae and 33 % in Ahnsankkae compared with that of film-mulching, therefore Yangbaeckkae was more adaptable for non-mulching than Ahnsankkae. Main factors decreasing yield of Yangbaeckkae under non-mulching were small LAI, NAR, and CGR at the stage of young seedling, and small number of capsules at early maturing stage from first flowering to 20 days after first flowering.

• Journal of Microbiology and Biotechnology
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• 제20권12호
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• pp.1724-1734
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• 2010

A phosphate-solubilizing bacterial strain isolated from Hippophae rhamnoides rhizosphere was identified as Rahnella sp. based on its phenotypic features and 16S rRNA gene sequence. The bacterial strain showed the growth characteristics of a cold-adapted psychrotroph, with the multiple plant growth-promoting traits of inorganic and organic phosphate solubilization, 1-aminocyclopropane-1-carboxylate-deaminase activity, ammonia generation, and siderophore production. The strain also produced indole-3-acetic acid, indole-3-acetaldehyde, indole-3-acetamide, indole-3-acetonitrile, indole-3-lactic acid, and indole-3-pyruvic acid in tryptophan-supplemented nutrient broth. Gluconic, citric and isocitric acids were the major organic acids detected during tricalcium phosphate solubilization. A rifampicin-resistant mutant of the strain exhibited high rhizosphere competence without disturbance to the resident microbial populations in pea rhizosphere. Seed bacterization with a charcoal-based inoculum significantly increased growth in barley, chickpea, pea, and maize under the controlled environment. Microplot testing of the inoculum at two different locations in pea also showed significant increase in growth and yield. The attributes of cold-tolerance, high rhizosphere competence, and broad-spectrum plant growth-promoting activity exhibited the potential of Rahnella sp. BIHB 783 for increasing agriculture productivity.

• 한국약용작물학회지
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• 제22권4호
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• pp.306-312
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• 2014

Endophytes are microorganisms that live in the internal tissues of plants without harming the host plants. In this symbiotic relationship, the host plants provide nutrients and shelter to the endophytes, in turn, endophytes can promote the growth of host plants and act as a biological control agents against plant pathogens. Plant-microbe interactions like this are noted for natural methods for sustainable agriculture and environmental conservation. However, in spite of the infinite potential, there are only a few reports on the endophytes present in ginseng. In this study, we isolated and identified the endophytes from Panax ginseng seeds and evaluated the biological activities (IAA production ability, nitrogen fixation ability, phosphate solubilization capacity, siderophore production ability, and antifungal activities) of the endophyte isolates. Eight different endophytes were identified by 16S rRNA sequencing. Most of the endophytes have antibiotic and plant growth promoting (PGP) activities. Particularly, PgSEB5-37E have the highest antibiotic activity, both PgSEB5-37B and PgSEB5-37H have high PGP traits such as an abilities to produce IAA, solubilize phosphate and fix nitrogen. These results indicated that the endophytes from P. ginseng seeds may have applicable value to many industries. In order to use the isolated endophytes, quantitative analysis and field tests are needed to be performed.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.207-207
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• 2022

Eating and cooking quality (ECQ) is one of the most complex quantitative traits in rice. The understanding of genetic regulation of transcript expression levels attributing to phenotypic variation in ECQ traits is limited. We integrated whole-genome resequencing, transcriptome, and phenotypic variation data from 84 Japonica accessions to build a transcriptome-wide association study (TWAS) based regulatory network. All ECQ traits showed a large phenotypic variation and significant phenotypic correlations among the traits. TWAS analysis identified a total of 285 transcripts significantly associated with six ECQ traits. Genome-wide mapping of ECQ-associated transcripts revealed 66,905 quantitative expression traits (eQTLs), including 21,747 local eQTLs, and 45,158 trans-eQTLs, regulating the expression of 43 genes. The starch synthesis-related genes (SSRGs), starch synthase IV-1 (SSIV-1), starch branching enzyme 1 (SBE1), granule-bound starch synthase 2 (GBSS2), and ADP-glucose pyrophosphorylase small subunit 2a (OsAGPS2a) were found to have eQTLs regulating the expression of ECQ associated transcripts. Further, in co-expression analysis, 130 genes produced at least one network with 22 master regulators. In addition, we developed CRISPR/Cas9-edited glbl mutant lines that confirmed the role of alpha-globulin (glbl) in starch synthesis to validate the co-expression analysis. This study provided novel insights into the genetic regulation of ECQ traits, and transcripts associated with these traits were discovered that could be used in further rice breeding.

• 정보통신설비학회논문지
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• 제9권3호
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• pp.102-106
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• 2010

Recently process industries from oil and gas procedures and mining companies to manufactures of chemicals, foods, and beverages has been exploring the USN (Ubiquitous Sensor Networks) technology to improve safety of production processes. However, to apply the USN technology in the large-scale plant industry, reliability and security issues are not fully addressed yet, and the absence of the industrial sensor networking standard causes a compatibility problem with legacy equipment and systems. Although this situation, process industry such as energy plants are looking for the secure wireless plant solution to provide detailed, accurate safety monitoring from previously hard-reach, unaccordable area. In this paper, SPSF (Smart Plant Safety Framework based on Reliable-Secure USN) is suggested to fulfill the requirements of high-risk industrial environments for highly secure, reliable data collection and plant monitoring that is resistant to interference. The SPSF consists of three main layers: 1) Smart Safety Sensing Layer, 2) Smart Safety Network Layers, 3) Plant Network System Layer.

• 한국연초학회지
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• 제27권2호
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• pp.153-162
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• 2005

Tobacco (Nicotiana tabacum L.) cells were transformed with rice expansin genes, OsEXPA4, OsEXPB3, OsEXPB4, and OsEXPB6, to elucidate the function of the genes in tobacco cells. The transformation increased the mass of the callus by $36\%-65 \%$, and the cell length by $12\%-28\%$. The cell width was decreased by $3\%$ for OsEXPB3, not changed for OsEXPB4, increased by $25\%\;and\;20\%$ for OsEXPA4 and OsEXPB6, respectively. From database search, seven expansin genes were found and six of them belong to EXPA group and one of them belongs to EXPB group. EXLA and EXLB were not found. All tobacco expansin genes were evenly distributed in the phylogenetic tree of rice and Arabidopsis expansin genes.

• 산업경영시스템학회지
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• 제47권3호
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• pp.68-74
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• 2024

This study develops a model to determine the input rate of the chemical for coagulation and flocculation process (i.e. coagulant) at industrial water treatment plant, based on real-world data. To detect outliers among the collected data, a two-phase algorithm with standardization transformation and Density-Based Spatial Clustering of Applications with Noise (DBSCAN) is applied. In addition, both of the missing data and outliers are revised with linear interpolation. To determine the coagulant rate, various kinds of machine learning models are tested as well as linear regression. Among them, the random forest model with min-max scaled data provides the best performance, whose MSE, MAPE, R² and CVRMSE are 1.136, 0.111, 0.912, and 18.704, respectively. This study demonstrates the practical applicability of machine learning based chemical input decision model, which can lead to a smart management and response systems for clean and safe water treatment plant.