• Journal of Applied Biological Chemistry
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• v.58 no.4
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• pp.303-309
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• 2015

${\gamma}$-Aminobutyric acid (GABA) content in fermented plant products and their main plant materials (aerial part of Acanthopanax sessiliflorus, fruit of Crataegus pinnatifida, and whole plant of Morus alba) was determined by high-performance liquid chromatography. GABA was quantified using a reverse-phase column with a gradient elution program (water:acetonitrile =90:10 to 0:100 for 40 min). UV detection was conducted at 280 nm. GABA content was measured in fermented plant products (15.07 mg/g), aerial part of A. sessiliflorus (4.49 mg/g), fruit of C. pinnatifida (10.59 mg/g), and whole plant of M. alba (2.31 mg/g). The presence of GABA in fermented plant products, including A. sessiliflorus, C. pinnatifida, and M. alba is important in industrial application for health supplements.

• Journal of Plant Biotechnology
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• v.43 no.2
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• pp.181-188
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• 2016

In this study, we developed 15 novel polymorphic simple sequence repeat (SSR) markers by SSR-enriched genomic library construction from Codonopsis lanceolata. We obtained a total of 226 non-redundant contig sequences from the assembly process and designed primer sets. These markers were applied to 53 accessions representing the cultivated C. lanceolata in South Korea. Fifteen markers were sufficiently polymorphic, and were used to analyze the genetic relationships between the cultivated C. lanceolata. One hundred three alleles of the 15 SSR markers ranged from 3 to 19 alleles at each locus, with an average of 6.87. By cluster analysis, we detected clear genetic differences in most of the accessions, with genetic distance varying from 0.73 to 0.93. Phylogenic analysis indicated that the accessions that were collected from the same area were distributed evenly in the phylogenetic tree. These results indicate that there is no correlative genetic relationship between geographic areas. These markers will be useful in differentiating C. lanceolata genetic resources and in selecting suitable lines for a systemic breeding program.

• Journal of Plant Biotechnology
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• v.48 no.2
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• pp.100-105
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• 2021

Genetic engineering is a potential approach to improve secondary metabolism in plants. In order to elucidate the effect of production of anthocyanin pigment 1 (PAP1) overexpression on the bioactivity of ginseng, we analyzed its antioxidant, antimicrobial, and anti-elastase activities in this study. Our results showed that PAP1 overexpression increased the production of polyphenolic compounds including anthocyanins. The antioxidant, antimicrobial, and anti-elastase activities were stronger in anthocyanin-overproducing ginseng hairy roots (AOX) than in wild ginseng hairy roots. Using a different solvent system (0, 30, 70, and 100% (v/v) EtOH), we revealed that variations in the contents of the polyphenolic compounds were highly correlated with changes in the antioxidant and antimicrobial activities of AOX. The antioxidant, antimicrobial, and anti-elastase effects of AOX highlight genetic engineering as a powerful approach to enhance the therapeutic properties of plants. Our results show that AOX could potentially have various functional applications in the cosmetic and pharmaceutical industries.

• IE interfaces
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• v.15 no.4
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• pp.364-373
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• 2002

This paper developed an operating software for the manufacturing cell of a model plant. The model plant, which was manufactured by Fishertechnik, consists of an assembly cell, a machining cell, and supplementary material handling equipments. Each I/O module of the model plant communicates with the INTERBUS controller devices via High-level Language Interface(HLI). The machining center has two machining cells, which consists of 4 machines respectively, two 3-axis portal transporters, and a rail guided vehicle(RGV). The Petri-Net was used to investigate the dynamics of each machining cell, e.g., the relation of material handling equipments and machines. The operating software was analyzed and designed by Object Oriented Technique. The software was implemented using Delphi 3.0 under Windows 95/NT operating system.

• 한국약용작물학회:학술대회논문집
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• 2011.04a
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• pp.318-319
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• 2011

• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.125-125
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• 2023

• Proceedings of the Korean Society of Crop Science Conference
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• 2023.04a
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• pp.130-130
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• 2023

• Journal of the Korean Society of Industry Convergence
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• v.22 no.1
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• pp.55-60
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• 2019

Growing plant in potting media without soil is known as Soilless cultivation. This method is used mostly in greenhouse cultivation to increase horticultural commodities production. Peat moss is commonly utilized as potting media substrate because of its characteristic. However, peat moss price is high because of the quantity of peat moss in nature has been decreased. Recently, most of the research is conducted to find the alternative growing medium to cultivate horticulture plant in potting media. Perlite and rice husk ash were mentioned that had a potent as alternative growing media for seasonal plants to increase agriculture production due to the lack of production area. This research aims to determine the growth of in rice husk ash, perlite and peat moss as growing substrates. The method used was the soilless cultivation. The chinese cabbage was planted in the pot with perlite media, rice husk ash media, and peat moss media. The chinese cabbage was measured after 35 days after planting. The result showed that peatmoss was more potentials in chinese cabbage growth performance than rice husk ash and perlite. Peat moss had the significant result of every research parameters such as plant height, plant weight, number of leaves, plant diameter, root length, and root weight. The best alternative for cultivation chinese cabbage without substrate based on this research was peat moss then rice husk ash and perlite.

• Journal of Plant Biotechnology
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• v.50
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• pp.232-238
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• 2023

Histone deacetylases (HDACs) play a pivotal role in epigenetic regulation, affecting the structure of chromatin and gene expression across different stages of plant development and in response to environmental stresses. Although the role of HDACs in Arabidopsis and rice has been focused on in extensive research, the role of the HDAC gene family in various medicinal plants remains unclear. In the genome of the balloon flower (Platycodon grandiflorus), we identified 10 putative P. grandiflorus HDAC (PlgHDAC) proteins, which were classified into the three families (RPD3/HDA1, SIR2, and HD2 HDAC families) based on their domain compositions. These HDACs were predicted to be localized in various cellular compartments, indicating that they have diverse functions. In addition, the tissue-specific expression profiles of PlgHDACs differed across different plant tissues, indicating that they are involved in various developmental processes. Furthermore, the expression levels of all PlgHDACs were upregulated in leaves after waterlogging treatment, implying their potential role in coping with waterlogging-induced stress. Overall, our findings provide a comprehensive foundation for further research into the epigenetic regulation of PlgHDACs, and particularly, on their functions in response to environmental stresses such as waterlogging. Understanding the roles of these HDACs in the development and stress responses of balloon flower could have significant implications for improving crop yield and the quality of this important medicinal plant.

• Korean Journal of Plant Resources
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• v.20 no.3
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• pp.263-266
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• 2007

High performance liquid chromatography (HPLC) was used for the analysis of chiisanoside in each stem and root of Acanthopanax senticosus collected from South Korea, North Korea, China and Russia. A reverse-phase system using a gradient of H$_{2}$O and acetonitrile as the mobile phase was developed and detection was at 210nm. The analysis was successfully carried out within 30 min. Chiisanoside was measured in the stem and root of A. senticosus collected from various countries.