Determination of Authentic Chiisanoside in Acanthopanax senticousus by High Performance Liquid Chromatography

  • Lee, Jeong-Min (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University) ;
  • Kim, Min-Hye (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University) ;
  • Lee, Sang-Hyun (Department of Applied Plant Science, College of Industrial Science, Chung-Ang University)
  • Published : 2007.06.30

Abstract

High performance liquid chromatography (HPLC) was used for the analysis of chiisanoside in each stem and root of Acanthopanax senticosus collected from South Korea, North Korea, China and Russia. A reverse-phase system using a gradient of H$_{2}$O and acetonitrile as the mobile phase was developed and detection was at 210nm. The analysis was successfully carried out within 30 min. Chiisanoside was measured in the stem and root of A. senticosus collected from various countries.

Keywords

References

  1. Bae E.A., C.S. Yook, O.J. Oh, S.Y. Chang, T. Nohara and D.H. Kim. 2001. Metabolism of chiisanoside from Acanthopanax divaricatus var. albeofructus by human intestinal bacteria and its relation to some biological activities. Biol. Pharm. Bull. 24: 582- 585 https://doi.org/10.1248/bpb.24.582
  2. Davydov M. and A.D. Krikorian. 2000. Eleutherococcus senticosus (Rupr. & Maxim.) Harms (Araliaceae) as an adoptogen: a closer look. J. Ethnopharmacol. 72: 345-393 https://doi.org/10.1016/S0378-8741(00)00181-1
  3. Hahn D.R., R. Kasai, J.H. Kim, S. Taniyasu and O. Tanaka. 1984. A new glycosyl ester of a 3,4-seco-triterpene from Korean medicinal plant, Acanthopanax chiisanensis. Chem. Pharm. Bull. 32: 1244-1247 https://doi.org/10.1248/cpb.32.1244
  4. Jiang W., W. Li, L. Han, L. Liu, Q. Zhang, S. Zhang, T. Nikaido and K. Koike. 2006. Biologically active triterpenoid saponins from Acanthopanax senticosus. J. Nat. Prod. 69: 1577-1581 https://doi.org/10.1021/np060195+
  5. Jin J.L., S. Lee, Y.Y. Lee, J.M. Kim, J.E. Heo and H.S. Yun-Choi. 2004. Platelet anti-aggregating triterpenoids from the leaves of Acanthopanax senticosus and the fruits of A. sessiliflorus. Planta Med. 70: 564-566 https://doi.org/10.1055/s-2004-827159
  6. Jung H.J., J.H. Nam, J. Choi, K.T. Lee and H.J. Park. 2005. Antiinflammatory effects of chiisanoside and chiisanogenin obtained from the leaves of Acanthopanax chiisanensis in the carrageenan- and Freund's complete adjuvant-induced rats. J. Ethnopharmacol. 97: 359-367 https://doi.org/10.1016/j.jep.2004.11.026
  7. Kang J.S., P.T. Linh, X.F. Cai, J.J. Lee and Y.H. Kim. 2003. Determination of chiisanoside in Acanthopanax species by high performance liquid chromatography. Nat. Prod. Sci. 9: 45-48
  8. Kim C.J. and D.R. Hahn. 1980. The biological activity of a new glycoside, chiisanoside from Acanthopanax chiisanensis Nakai leaves. Yakhak Hoeji 24: 123-134
  9. Kim Y.O., D.H. Cho, H.J. Chung, J.H. Kim, S.Y. Chang, C.S. Yook, K.S. Yang and O.J. Oh. 1999. Effects of lupinetriterpenoids on mitogen-induced proliferation of lymphocytes. Yakhak Hoeji 43: 208-239
  10. Lee S., D.S. Shin, K.B. Oh and K.H. Shin. 2003. Antibacterial compounds from the leaves of Acanthopanax senticosus. Arch. Pharm. Res. 26: 40-42 https://doi.org/10.1007/BF03179929
  11. Perry L.M. and J. Metzger. 1980. Medicinal plants of East and Southeast Asia. MIT press, Cambridge, Massachusetts and London pp. 41
  12. Shin K.H. and S. Lee. 2002. The chemistry of secondary products from Acanthopanax species and their pharmacological activities. Nat. Prod. Sci. 8: 111-126
  13. Shin S.-J. and S.-T. Hong. 2005. Acanthopanax and Platycodi independently prevents the onset of high fat diet induced hyperglyceridemia and obesity in C57BL/6 mice. Food Sci. Biotechnol. 14: 841-846
  14. Won J.H., S.Y. Park, S.G. Nam, H.J. Park, J.W. Choi and K.T. Lee. 2005. Inhibition of lipopolysaccharide-induced expression of inducible nitric oxide and cyclooxygenase-2 by chiisanoside via suppression of nuclear factor-kappaB activation in RAW 264.7 macrophage cells. Biol. Pharm. Bull. 28: 1919-1924 https://doi.org/10.1248/bpb.28.1919
  15. Yook C.S., Y.S. Rho, S.H. Seo, J.Y. Leem and D.R. Han. 1996. Chemical components of Acanthopanax divaricatus and anticancer effect in leaves. Yakhak Hoeji 40: 251-261
  16. Yook C.S. 1990. Coloured medicinal plants of Korea. Academy Publishing Co., Seoul pp. 377
  17. Yoshizumi K., K. Hirano, H. Ando, Y. Hirai, Y. Ida, T. Tsuji, T. Tanaka, K. Satouchi and J. Terao. 2006. Lupane-type saponins from leaves of Acanthopanax sessiliflorus and their inhibitory activity on pancreatic lipase. J. Agric. Food Chem. 54: 335-341 https://doi.org/10.1021/jf052047f