• Title/Summary/Keyword: Induced Strain

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Cytogenetic Analysis of All-Female Triploid Olive Flounder Paralichthys olivaceus for Ploidy Verification (전 암컷 3배체 넙치(Paralichthys olivaceus)에 대한 효율적인 세포유전학 분석법)

  • Ko, Min Gyun;Jung, Hyo Sun;Lee, Hyo Bin;Kim, Dong Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.49 no.5
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    • pp.671-674
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    • 2016
  • We cytogenetically analyzed a triploid King-Nupchi strain of the olive flounder Paralichthys olivaceus to define the simplest, most rapid, and most effective method of ploidy analysis in aquaculture farms. Female triploidy of the flounder King-Nupchi strain was induced by cold shock (3 min post-fertilization at 2-4℃ for 45 min). Triploid induction was confirmed by erythrocyte measurement (nuclear volume, 29.15±2.10 μm3); flow cytometry (2.14±0.03 pg/cell); chromosome count (3N=72); Ag-NOR banding; and silver staining. Silver staining of finned cells obtained using a solid tissue technique was the most effective method of ploidy verification.

A Study on PECVD Silicon Nitride Thin Films for IC Chip Packaging (IC 칩 패키지용 PECVD 실리콘 질화막에 관한 연구)

  • 조명찬;정귀상
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1996.05a
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    • pp.220-223
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    • 1996
  • Mechanical properties of Plasma-Enhanced Chemical Vapor Deposited (PECVD) silicon nitride thin film was studied to determine the feasibility of the film as a passivation layer over the aluminum bonding areas of integrated circuit chips. Ultimate strain of the films in thicknesses of about 5 k${\AA}$ was measured using four-point bending method. The ultimate strain of these films was constant at about 0.2% regardless of residual stress. Intrinsic and residual stresses of these films were measured and compared with thermal shock and cycling test results. Comparison of the results showed that more tensile films were more susceptible to crack- induced failure.

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An Experimental Study on the Impact Force of a Mechanical HeBrt Valve Prostheses (기계식 심장판막의 충격력에 관한 실험적 연구)

  • 이정한;천길정
    • Journal of Biomedical Engineering Research
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    • v.17 no.2
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    • pp.215-220
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    • 1996
  • Impact force and strains induced by impact between the occluder and the struts have been measured with force sensor and strain gages. The maximum reaction force was about 25N, and the calculated impact force on the root of the struts amount about 9-17W. Impact force on the inlet strut is greater than that of the outlet strut, but the strain on the outlet strut is much higher than that of the inlet strut. These values might cause severe damage on the valve in the critical cases. The results of this study may be extended for the analysis of the endurance limit and optimal design of the struts and occluder.

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Regulation of fpr Gene Encoding NADPH : Ferredoxin Oxidoreductase by the soxRS Locus in Escherichia coli

  • Koh, Young-Sang;Choih, Jenny;Roe, Jung-Hye
    • Journal of Microbiology
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    • v.34 no.2
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    • pp.137-143
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    • 1996
  • We isolated a promoter inducible by paraquat, a superoxide-generating agent, from Escherichia coli using a promoter-probing plasmid pRS415. From sequence analysis we found out the promoter is for fpr ENCODING nadph : ferredoxin oxidoreductase. We constructed on operon fusion of lacZ gene with fpr promoter to monitor the expression of the gene in the single-copy state. LacZ expression generators, menadione and plumbagin, also induced the expression of .betha.-galactosidase in the fusion strain. On the other hand, no significant induction was observed by treatment with hydrogen peroxide, ethanol, and heat shock. Induction of .betha.-galactosidase was significantly reduced by introducing a .DELTA. sox 8 :: cat of soxS3 :: Tn10 mutation into the fusion strain, indicating that fpr gene is a member of the soxRS regulon. The transcriptional start site was determined by primer extension analysis. Possible roles of fpr induction in superoxide stress were discussed.

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Effect of Mn-Addition on the Cavitation Erosion Resistance of Surface-hardened Fe-20Cr-1Si-1C Aolly (Fe-20Cr-1Si-1C계 경면처리 합금의 cavitation erosion 저항성에 미치는 Mn 첨가의 영향)

  • 김수철;박인호;장세기;김선진;강성군
    • Journal of the Korean institute of surface engineering
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    • v.33 no.1
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    • pp.10-16
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    • 2000
  • Characteristics of cavitation erosion resistance of Fe-20Cr-1Si-1C-xMn(x=0, 5, 15) alloys were investigated by SEM and XRD analysis. The effects on strain-induced transformations were considerably reduced with increasing the amounts of Mn due to twining that occurred at 5, 15Mn alloys, activating cavitation erosion rates(mg/$\textrm{cm}^2$) which varied as 0.055, 0.114 and 0.160mg/$\textrm{cm}^2$ for 0, 5, 15Mn. From the results, it was found that the addition of Mn element in Fe-base alloy provides more cracking sites at twins rather than absorbing strain energies, so accelerates cavitation erosion rates.

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Development & Verification of Frequency-Strain Dependence Curve (주파수-변형률 곡선의 개발 및 검증)

  • Jeong, Chang-Gyun;Kwak, Dong-Yeop;Park, Du-Hee
    • Proceedings of the Korean Geotechical Society Conference
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    • 2009.03a
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    • pp.146-153
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    • 2009
  • One dimensional site response analysis is widely used in prediction of the ground motion that is induced by earthquake. Equivalent linear analysis is the most widely used method due to its simplicity and ease of use. However, the equivalent linear method has been known to be unreliable since it approximates the nonlinear soil behavior within the linear framework. To consider the nonlinearity of the ground at frequency domain, frequency dependent algorithms that can simulate shear strain - frequency dependency have been proposed. In this study, the results of the modified equivalent linear analysis are compared to evaluate the degree of improvement and the applicability of the modified algorithms. Results show the novel smoothed curve that is proposed by this study indicates the most stable prediction and can enhance the accuracy of the prediction.

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A multivariate adaptive regression splines model for estimation of maximum wall deflections induced by braced excavation

  • Xiang, Yuzhou;Goh, Anthony Teck Chee;Zhang, Wengang;Zhang, Runhong
    • Geomechanics and Engineering
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    • v.14 no.4
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    • pp.315-324
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    • 2018
  • With rapid economic growth, numerous deep excavation projects for high-rise buildings and subway transportation networks have been constructed in the past two decades. Deep excavations particularly in thick deposits of soft clay may cause excessive ground movements and thus result in potential damage to adjacent buildings and supporting utilities. Extensive plane strain finite element analyses considering small strain effect have been carried out to examine the wall deflections for excavations in soft clay deposits supported by diaphragm walls and bracings. The excavation geometrical parameters, soil strength and stiffness properties, soil unit weight, the strut stiffness and wall stiffness were varied to study the wall deflection behaviour. Based on these results, a multivariate adaptive regression splines model was developed for estimating the maximum wall deflection. Parametric analyses were also performed to investigate the influence of the various design variables on wall deflections.

Thermosensing of Thermotactic Mutants, Dictyostelium discoideum Amoebae in Vegetative Stage

  • Hong, Choo-Bong
    • Journal of Plant Biology
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    • v.26 no.3
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    • pp.131-139
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    • 1983
  • Temperature response of amoebae of thermotactic mutants have been investigated. Amoebae of the mutant strain HO 428 showed positive thermotaxs which is strong at lower temperaturs and drops sharply above the growth temperature of amoebae. The temperature response of HO 428 amoebae was not affected by the length of amoebae on the grdients. HO 596 amoebae seemed to have both positive and negative thermotactic responses shortly after food depletion. Longer exposure of these amoebae on the thermal gradients induced a stronger negative response at lower temperatures and an apparent positive response at higher temperatures. A similar changes could be observed in HO 1445 amoebae. Based on the steady positive thermotactic response by HO 428 amoebae and the mode of change in termperature response at higher temperatures, 24$^{\circ}C$ and $25^{\circ}C$, by HO 596 amoebae, a model for the temperature response of vegetative Dictyostelium discoideum amoebae, strain HL 50, has been proposed. The main features of the model are: a positive response at the thermal gradients with midpoint temperatures lower than the growth temperatures of amoebae and a negative response above it.

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Field-Induced Strain and Polarization Characteristics of La-Modified PMN-PT Ceramics (La 변성 PMN-PT계 강유전체의 전계유기변위와 분극특성)

  • Kim, Myeong-Cheol
    • Korean Journal of Materials Research
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    • v.7 no.7
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    • pp.547-558
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    • 1997
  • Pb(Mg$_{1}$3/Nb$_{2}$3)O$_{3}$[PMN]-PbTiO$_{3}$[PT]계 고용체의 상경계조성(MPB)영역에 대해 La을 첨가하여 변성시킨 La변성 (1-x)PMN-xPT(x=0.35) 고용체를 만들어 온도-유전율 특성, 전계유기 분극특성 및 변위특성을 조사하였다. PMN-PT의 상경영역의 조성인 x=0.35에 대해 La함량을 0-10at%까지 변화시켰다. PMN-PT계 고용체에서 PT 의 함량이 증가함에 따라 전계유기변위 및 히스테리시스 특성이 모두 증가하였다. 전계유기변위 값 $\varepsilon$ 는 능면정과 정방정의 공존영역인 MPB(x=0.35)조성에서 가장 높은 값($\varepsilon$ = 2 x $10^{-3}$)을 보여 주었다. MPB조성에 대해 La을 첨가한 계의 전계유기변위를 조사한 결과 La의 첨가량이 적을 때 (La=0-5at%)는 La의 함량이 증가함에 따라 히스테리시스 특성이 감소하였고 $\varepsilon$ 은 증가하여 고성능의 액튜에이터 재료에 적합한 조성물로 기대된다.

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Sensitivity Improvement of Fiber Bragg Grating Temperature Sensor (광섬유격자 온도센서의 민감도 개선)

  • Kang, Han-Chul;Song, Min-Ho
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.26 no.3
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    • pp.40-45
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    • 2012
  • We substantially increased the temperature sensitivity of a fiber Bragg grating temperature sensor by gluing it onto a piece of bi-metal strip. The temperature-induced Bragg wavelength shift is increased upto 5 times more than that of the intrinsic FBG sensor by the strain effect from the deflected bi-metal. We showed the feasibility of the proposed sensor by comparing the temperature measuring experiments with those of the intrinsic FBG sensors. Also we measured temperature varying the length of the bi-metal pieces and analyzed the result effect of it.