• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1239-1248
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• 2004

The methylotrophic yeast Hansenula polymorpha has been extensively studied as a model organism for methanol metabolism and peroxisome biogenesis. Recently, this yeast has also attracted attention as a promising host organism for recombinant protein production. Here, we describe the fabrication and evaluation of a DNA chip spotted with 382 open reading frames (ORFs) of H. polymorpha. Each ORF was PCR-amplified using gene-specific primer sets, of which the forward primers had 5'-aminolink. The PCR products were printed in duplicate onto the aldehyde-coated slide glasses to link only the coding strands to the surface of the slide via covalent coupling between amine and aldehyde groups. With the partial genome DNA chip, we compared efficiency of direct and indirect cDNA target labeling methods, and found that the indirect method, using fluorescent-labeled dendrimers, generated a higher hybridization signal-to-noise ratio than the direct method, using cDNA targets labeled by incorporation of fluorescence-labeled nucIeotides during reverse transcription. In addition, to assess the quality of this DNA chip, we analyzed the expression profiles of H. polymorpha cells grown on different carbon sources, such as glucose and methanol, and also those of cells treated with the superoxide­generating drug, menadione. The profiles obtained showed a high-level induction of a set of ORFs involved in methanol metabolism and oxidative stress response in the presence of methanol and menadione, respectively. The results demonstrate the sensitivity and reliability of our arrays to analyze global gene expression changes of H. polymorpha under defined environmental conditions.

• Journal of dental hygiene science
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• v.15 no.4
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• pp.407-412
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• 2015

This study investigated flexural properties of indirect Gum-shade composite resins for esthetic improvement. The material utilized in this study was Crea.lign, Twiny flow and Twiny paste (TP). Ten specimens were fabricated with a dimension of $25{\times}2{\times}2mm$ according to the ISO 4049. After fabrications, specimens were stored in the distilled water for 24 hours at the temperature of $37^{\circ}C$. Three-point bending test was performed in universal testing machine (Instron 3344; Instron, USA) at a crosshead speed of 1 mm/min until the failure occurred. TP exhibited a higher flexural strength (FS) and flexural modulus (FM) compared to the flowable materials. There were significant differences among the three materials in FS and FM. However, there was no significant difference in work of fracture (WOF) in all tested materials (p>0.05). In Weibull analysis, TP showed the greatest Weibull modulus which means a higher reliability of the materials. Also, Gum-shade composite resins revealed a strong correlation in all flexural properties. There was a positive correlation in FS-FM ($r^2=0.99$) and a negative correlation between FS-WOF and FM-WOF ($r^2>0.97$). Therefore, this confirmed that flexural property was important for mechanical behavior evaluation and useful information. To addition, this improved among mechanical properties correlation of materials as important factor.

• Journal of Korean Tunnelling and Underground Space Association
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• v.21 no.4
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• pp.501-518
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• 2019

The rock properties measured under in-situ geological condition can be used to increase the reliability in numerical simulations with regard to the long-term performance of a high-level waste repository. In this study, the change in mechanical properties of KURT (Korea atomic energy research institute Underground Research Tunnel) granite was evaluated under the simulated THM (Thermo-Hydro-Mechanical) coupled condition due to a deep geological formation in the disposal repository. The rock properties such as uniaxial compression strength, indirect tensile strength, elastic modulus and Poisson's ratio were measured under the coupled test conditions (M, HM, TM, THM). It was found that the mechanical properties of KURT granite is more susceptible to the change in saturation rather than temperature within the test condition of this study. The changes in uniaxial compression strength and indirect tensile strength from the rock samples of dried or saturated conditions showed the maximum relative error of about 20% and 13% respectively under the constant temperature condition. Therefore, it is necessary to use the material properties of rock measured under the coupled THM condition as input parameters for the numerical simulation of long-term performance assessment of a disposal repository

• Journal of Korea Water Resources Association
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• v.55 no.6
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• pp.461-469
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• 2022

It is very important to ensure the reliability of dam inflow data, which is critical in planning and managing the supply and demand of water resources in a basin. However, the simple water balance model sometimes results in negative inflows and does not consider the actual inflow characteristics. In this study, to address these issues, the existing water balance formula was modified by considering evaporation which is available for calculation among other outflows. The modified water balance formula was applied to the Soyang Dam. The results showed that the rate of negative inflows decreased in the re-evaluated dam inflow data and it was possible to secure consistency for the total inflow volume. In addition, investigating the water availability in the Soyang Dam watershed based on the water balance concept considering evaporation, it was found that direct water use in the human aspect was about 60%, and the indirect water use in the natural aspect was about 40%. In drought years, it was also confirmed that the proportion of indirect use of water resources increased.

• Journal of Internet of Things and Convergence
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• v.8 no.5
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• pp.11-16
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• 2022

As an interest in the development of artificial intelligence(AI) technology in the water supply sector increases, we have developed an AI algorithm that can predict improvement decision-making ratings through repetitive learning using the data of pipe condition evaluation results, and present the most reliable prediction model through a verification process. We have developed the algorithm that can predict pipe ratings by pre-processing 12 indirect evaluation items based on the 2020 Han River Basin's basic plan and applying the AI algorithm to update weighting factors through backpropagation. This method ensured that the concordance rate between the direct evaluation result value and the calculated result value through repetitive learning and verification was more than 90%. As a result of the algorithm accuracy verification process, it was confirmed that all water pipe type data were evenly distributed, and the more learning data, the higher prediction accuracy. If data from all across the country is collected, the reliability of the prediction technique for pipe ratings using AI algorithm will be improved, and therefore, it is expected that the AI algorithm will play a role in supporting decision-making in the objective evaluation of the condition of aging pipes.

• The Journal of the Korea Contents Association
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• v.21 no.12
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• pp.632-644
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• 2021

The purpose of this study was to examine the mediating effect of ego-resilience and countertransference management ability in the relationship between counselor's adult attachment and development levels of counselors. For this purpose, 389 counselors who conducted more than 5 times of verbal counseling per case were surveyed for 3 weeks using the online questionnaire function. Data were analyzed using SPSS 21 and AMOS 21, reliability analysis was conducted, and constructed structural equation model(SEM) and to verify validity, fitness, and mediating effects. The results of the analysis are as follows: First, counselor's attachment anxiety and attachment avoidance had indirect effects on the ability to manage countertransference through the medium of ego-resilience(p<.001). Second, counselor's attachment avoidance had an indirect effect on counselor's developmental level through countertransference management ability(p<.05). Third, it was confirmed that counselor's attachment anxiety and attachment avoidance had an effect on counselor's development level through double mediation of ego-resilience and countertransference management ability(p<.001). The significance and limitations of these results and suggestions for follow-up studies were discussed.

• Journal of Venture Innovation
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• v.5 no.2
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• pp.85-99
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• 2022

Dryers becoming commercially available for experimental and industrial use are classified to general drying oven, hot-air dryer, vacuum dryer, freezing dryer, etc. and kinds of them are various from the function, size and volume, etc. But the moisture measurement is not applied although it is important factor for the quality control and the performance improvement of products, and then now is very passive because the weight is weighed arbitrarily after dry-end. Generally the method for measuring moisture is divided by a direct measurement method and a indirect measurement method, and the former such as the change of weight or volume on the front and rear of separation of moisture, etc. is mainly used. Relatively a indirect measurement is very limited to apply due to utilize measurement apparatuses using temperature conductivity and micro-wave etc. In this research, we easily designed the moisture measurement system using the open-source based Arduino, and monitored moisture fluctuations and weight profiles in the real-time without the effect of external environment. Concretely the temperature-humidity and load cell sensors were packaged into a drying oven and the various change values were measured, and their sensors capable to operate 60℃ and 80℃ were selected to suitable for the moisture sensitive materials and the food dry. And also the performance safety using the organic samples of banana, pear, sawdust could be secured because the changes of evaporation rate as the dry time and temperature, and the measurement values of load cell appeared stable response characteristics through repeated experiments. Hereafter we judge that the reliability can be improved increasingly through the expansion of temperature-humidity range and the comparative analysis with CFD(Computational Fluid Dynamics) program.

• Korean Journal of Culture and Social Issue
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• v.29 no.4
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• pp.477-496
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• 2023

The purpose of this study is to examine the relationship between rejection sensitivity and reactive aggression among college students, as well as to determine the mediating effects of self-concept clarity and hostile attribution bias on the relationship between rejection sensitivity and reactive aggression. A self-report questionnaire was conducted online for the purpose of gathering data from university students aged 18 years and older. A total of 250 participants were included in the analysis. SPSS 27.0 was used for data analysis to check the basic statistics of the variables, frequency analysis, reliability analysis, and correlation analysis. In addition, the model fit was checked using Amos 21.0, and the bootstrapping method verified the significance of the indirect effect. The results of this study are as follows. The results of this study are as follows. First, rejection sensitivity positively affects reactive aggression through self-concept clarity. Second, rejection sensitivity increases the hostile attribution bias, leading to an increase in reactive aggression. Third, rejection sensitivity positively influences reactive aggression in an indirect way by sequentially affecting self-concept clarity and hostile attribution bias. These findings have implications as they identify psychological factors that affect reactive aggression in college students. This suggests the importance of utilizing psychological interventions to address reactive aggression associated with social problems, such as crime, and provides a foundation for both treatment and prevention. Finally, implications for further research and limitations of this study are suggested.

• Industry Promotion Research
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• v.9 no.2
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• pp.103-113
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• 2024

This study aims to determine whether employment status moderates and mediates the effect of parenting stress on parenting efficacy through mental health and hope targeting women raising children and to find ways to improve parenting efficacy by utilizing mental health and hope. The survey was conducted nationwide, a purposive sampling method was used to select the survey subjects, and data were collected through a survey. The total number of subjects used in the final analysis was 300 women. Data were analyzed by applying frequency analysis, reliability analysis, correlation analysis, and moderated mediation effect analysis. The results of the study are as follows. First, as a result of correlation analysis, parenting stress showed a negative correlation with mental health, hope, employment status, and parenting efficacy. On the other hand, other variables except parenting stress showed positive correlations with each other. Second, employment status moderated and mediated the effect of parenting stress on parenting efficacy through mental health and hope. In other words, employment status played a buffering role in reducing the negative impact of parenting stress on parenting efficacy through mental health and hope. Based on these results, a plan to protect parenting efficacy was proposed.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2005.05a
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• pp.969-974
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• 2005

TPM program, which is a methodology for improving the management results through the management innovation activity of a company, has been widely introduced in the field of a service industry as well as a manufacturing industry. The main purpose of this study is to present the theoretical model by the relationship between active factors of TPM program and management results for a productivity increase, and to investigate the direct and indirect effects on the management of a company through a parametric study. A questionnaire survey of 300 companies that presently utilize the TPM program has been conducted. In order to verify the validity and the reliability of the contents of the questionnaire survey, a confirmatory factor analysis has been done. A frequency analysis has also been performed to examine the characteristics of the respondent. The factor analysis and the frequency analysis were done by using SAS Ver. 8.2, and the verification of a research model was done by using LISREL Ver. 8.52. The active factors of TPM in the research model consist of 'an independent preservation', 'an individual improvement', 'a planned reservation' and 'an education & training'. Among those active factors, the individual improvement and the education & training significantly influence on the facility efficiency and the organization and personnel management. The organization and personnel management has a greater influence on the management results. Therefore, the education and training for employes is most important for the improvement of the management results through TPM program, and the individual improvement activity is also important.