• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.3
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• pp.277-286
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• 1985

From a series of studies conducted on the local maize lines at the Agr. College of the Chungnam National Univ., a few maize lines with many tillers and ears per plant were identified and tentatively named as MET. The purpose of the study was to evaluate the MET lines, which were selfed for five generations, for silage purpose under the different plant densities. A hybrid, Suwon #19 and a synthetic variety, Puyo #3${\times}$#2, were included for comparison. Plant height at harvesting times showed no significant varietal differences. However, the MET lines were very slow in early plant growth compared to the hybrid or synthetic variety, probably due to inbreeding depression of the MET lines. Total fresh weight at the harvesting times was highest in the MET 1 line. The MET 1 line was about 2,000 kgr. per 10a. higher than the hybrid at the harvesting time. The highest fresh weight was obtained when grown under the plant density of 60 ${\times}$ 20cm. Total dry weight per unit area showed the same tendency as the fresh weight. Total dry weight of MET 1 line was about 2.4 tons per 10a., which was about 10% higher than the hybrid, Suwon #19. As the fresh weight, the total dry weight was also highest in the plant density of 60 ${\times}$ 20cm. The grain yield per 10 are of MET 1 was comparable to the grain yield of the hybrid, especially in the low plant density, 60 ${\times}$ 40cm. The average number of effective tillers of MET lines were 4.5, while the mean tiller number of the hybrid or synthetic variety were none. However, the lodging was one of the problem for growing MET lines. The 100 kernel weight of MET lines was about 9 gr., while the 100 kernel weight of the hybrid or synthetic variety was about 30 grm.

• Animal Bioscience
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• v.37 no.5
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• pp.795-806
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• 2024

Objective: Karan Fries (KF), a high-producing composite cattle was developed through crossing indicine Tharparkar cows with taurine bulls (Holstein Friesian, Brown Swiss, and Jersey), to increase the milk yield across India. This composite cattle population must maintain sufficient genetic diversity for long-term development and breed improvement in the coming years. The level of linkage disequilibrium (LD) measures the influence of population genetic forces on the genomic structure and provides insights into the evolutionary history of populations, while the decay of LD is important in understanding the limits of genome-wide association studies for a population. Effective population size (N_e) which is genomically based on LD accumulated over the course of previous generations, is a valuable tool for e valuation of the genetic diversity and level of inbreeding. The present study was undertaken to understand KF population dynamics through the estimation of N_e and LD for the long-term sustainability of these breeds. Methods: The present study included 96 KF samples genotyped using Illumina HDBovine array to estimate the effective population and examine the LD pattern. The genotype data were also obtained for other crossbreds (Santa Gertrudis, Brangus, and Beefmaster) and Holstein Friesian cattle for comparison purposes. Results: The average LD between single nucleotide polymorphisms (SNPs) was r² = 0.13 in the present study. LD decay (r² = 0.2) was observed at 40 kb inter-marker distance, indicating a panel with 62,765 SNPs was sufficient for genomic breeding value estimation in KF cattle. The pedigree-based N_e of KF was determined to be 78, while the N_e estimates obtained using LD-based methods were 52 (SNeP) and 219 (genetic optimization for N_e estimation), respectively. Conclusion: KF cattle have an N_e exceeding the FAO's minimum recommended level of 50, which was desirable. The study also revealed significant population dynamics of KF cattle and increased our understanding of devising suitable breeding strategies for long-term sustainable development.

• Animal Bioscience
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• v.37 no.7
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• pp.1168-1176
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• 2024

Objective: As a charismatic species, cashmere goats have rich genetic resources. In the Inner Mongolia Autonomous Region, there are three cashmere goat varieties named and approved by the state. These goats are renowned for their high cashmere production and superior cashmere quality. Therefore, it is vitally important to protect their genetic resources as they will serve as breeding material for developing new varieties in the future. Methods: Three breeds including Inner Mongolia cashmere goats (IMCG), Hanshan White cashmere goats (HS), and Ujimqin white cashmere goats (WZMQ) were studied. IMCG were of three types: Aerbas (AEBS), Erlangshan (ELS), and Alashan (ALS). Nine DNA samples were collected for each population, and they were genomically re-sequenced to obtain high-depth data. The genetic diversity parameters of each population were estimated to determine selection intensity. Principal component analysis, phylogenetic tree construction and genetic differentiation parameter estimation were performed to determine genetic relationships among populations. Results: Samples from the 45 individuals from the five goat populations were sequenced, and 30,601,671 raw single nucleotide polymorphisms (SNPs) obtained. Then, variant calling was conducted using the reference genome, and 17,214,526 SNPs were retained after quality control. Individual sequencing depth of individuals ranged from 21.13× to 46.18×, with an average of 28.5×. In the AEBS, locus polymorphism (79.28) and expected heterozygosity (0.2554) proportions were the lowest, and the homologous consistency ratio (0.1021) and average inbreeding coefficient (0.1348) were the highest, indicating that this population had strong selection intensity. Conversely, ALS and WZMQ selection intensity was relatively low. Genetic distance between HS and the other four populations was relatively high, and genetic exchange existed among the other four populations. Conclusion: The Inner Mongolia cashmere goat (AEBS type) population has a relatively high selection intensity and a low genetic diversity. The IMCG (ALS type) and WZMQ populations had relatively low selection intensity and high genetic diversity. The genetic distance between HS and the other four populations was relatively high, with a moderate degree of differentiation. Overall, these genetic variations provide a solid foundation for resource identification of Inner Mongolia Autonomous Region cashmere goats in the future.

• Korean Journal of Agricultural Science
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• v.12 no.2
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• pp.166-173
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• 1985

The multiple ear and tiller line (MET), which was selected from a local maize collection and has been selected for earliness by selfing for five generations, was compared its plant characteristics with four U.S. leading hybrids. 1. The general plant growth was a little retarded by poor soil fertility and structure. 2. The fresh weight of the MET line was about 97.8% of the U.S. check varieties. 3. The dry weight of the MET line was also about 94.5% of the check U.S. hybrids (1,695 kg / 10a.). 4. The tillering and eating habits of the MET line had contributed toward increasing fresh and dry weight of the MET line. 5. The average plant height of the MET line was much less than those of the check varieties, due to the selfing depression manifested by the MET line, (234.7cm. of the MET vs 250.7cm. of the check) 6. Apparent differences of disease resistance was observed among varieties studied. The degree of plants infected with the black stripe mosaic virus was over 20% for the check U.S. hybrids, while less than 5% of the MET line was infected with the disease. 7. The grain yield per 10 a of the MET line was far below the check hybrids due to the inbreeding depression of the MET line. However, the kernel number per unit area of the MET line with small size kernel was much greater than that of the check hybrids. The 100 kernel weight of the MET line was around 10 grams. 8. The average fresh and dry matter weight of the three ($sorghum{\times}sudan$ grass) varieties was very much alike with those of the MET line. 9. In conclusion, the use of the MET line for silage production may have dual adventages because of its low seed price and of its small size kernels, which may reduce the amount of seeds required for planting in an unit area.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.spc1
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• pp.161-166
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• 2005

Objectives of this study were to select inbred lines which have a good eating quality and desirable segregates during inbreeding of waxy corn. The 64 inbred lines showed a large variance in their kernel shape and weight. 100-kernel weight, pericarp thickness, kernel length, kernel width, and kernel thickness ranged $11.7\~37.3g,\;11\~77{\mu}m,\;5.8\~9.6mm,\;6.5\~10.0mm$, and $4.1\~6.8mm$, respectively. The physicochemical analysis of 64 waxy corn inbred lines showed crude protein, crude fat, free sugar, and amylopectin content ranging $8.7\~15.8\%,\;2.3\~5.8\%,\;1.1\~11.0\%,\;and\;78.5\~93.8\%$, respectively. The texture property analysis of 64 inbred lines by texture analyzer showed a big difference. Gumminess, hardness, and chewiness of 64 inbred lines ranged $91\~383,\;181\~394,\;and\;73\~370$, respectively. The principal component analysis for 14 characteristics related to kernel quality showed that $73.1\%$ of the total variation could be attributed to the first five principal components. Biological meaning of the principal component was explained clearly by the correlation coefficient between principal components and characters. The first principal component appeared to correspond to small kernel and bad eating quality, The second principal component appeared to correspond to large kernel and good eating quality. The 64 inbred lines were classified into 8 groups by the cluster analysis using the first and second principal component. Among the groups, group VII and VIII included inbred lines with good eating quality that had thin pericarp thickness, low protein content, large kernel, and soft tenderness.

• Journal of Animal Science and Technology
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• v.53 no.5
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• pp.397-408
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• 2011

The study was carried to identify the impact on nation-wide genetic evaluation and to obtain basic materials for the development of strategies in Swine Improvement Network Project (SINP). Data consisted of pedigree records of 235,511 and performance records of 70,747 for Duroc from 1987 to 2010 were collected by Korea Animal Improvement Association. Performance traits included three point back fat thickness (Shoulder, Belly, Waist), loin area, days to 90 kg and average daily gain. Exchange of genetic resources cross the breeding farms was not high, and furthermore the sizable farms which can accommodate genetic evaluation within the farm were scarce. Three data sets (individual farm evaluation: I, two sub-group evaluation: S, and whole eight farm evaluation: P) were used for genetic analysis. Genetic variances were larger in subordinate farms than in joiners farms for connectedness, and consequently the heritabilities were generally higher in subordinate farms than in joiner farms with I. The standard errors of heritability were small in the order of I, S and P. Estimated average inbreeding coefficients were 1.12%, 0.95% and 1.53% for joiner and subordinate group with S and population with P, respectively. The estimated correlations of breeding values with I and P were lowest. The correlations of breeding values with I and P for traits ranged 0.22 to 0.45 for moved parent animals and 0.24 to 0.72 for all animals. The results in the study suggest that nation-wide evaluation uses more pedigree information and improves accuracy. Furthermore SINP for connectedness could help to improve the accuracy of evaluation.

• Korean Journal of Plant Resources
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• v.28 no.1
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• pp.101-110
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• 2015

Parameters of mating system and pollen flow of a Pinus densiflora population in Buan, South Korea, were estimated using seven nuclear microsatellite markers. The expected heterozygosity ($H_e$) was 0.614 in mother trees and 0.624 in seeds. Fixation index (F) was 0.018 and 0.087 in each generation. There was no significant genetic difference between the generations (P > 0.05). From MLTR, the outcrossing rate ($t_m$), the biparental inbreeding ($t_m-t_s$), and the correlation of paternity ($r_p$) were 0.967, 0.057, and 0.012, respectively. tm was larger but $t_m-t_s$ and $r_p$ were smaller than those of allozyme markers in Pinus densiflora. These values were similar to those of microsatellite markers in other pine species. The optimal pollen dispersal model from TwoGener was the normal dispersal model with the effective density of 220 trees/ha and its level of genetic differentiation in pollen pool structure (${\Phi}_{ft}$) was 0.021. The average radial distance of pollen flow (${\delta}$) was calculated as 11.42 m, but no correlation between the pairwise-${\Phi}_{ft}$ and the geographical distance among mother trees was at Mantel test (r = -0.141, P > 0.05). Although the effective pollen dispersal in the population seems to be restricted, the amount of genetic variation might be maintained in each generation without a loss of genetic diversity. It might be because the genetic diversity in pollen pool was high but the genetic difference between pollen donors was small under the complete random mating condition in the Pinus densiflora population in Buan.

• Korean Journal of Ichthyology
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• v.32 no.4
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• pp.210-221
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• 2020

Since the introduction of Israeli carp into Korea for farming in 1973, there are no breeding studies on developing Korea Israeli carp (domestic) so far. This study performed gene-based cross-breeding studies to restore genetic diversity of lowered Israeli carp through continuous inbreeding, and for rapid growth and better scales. This study produced four cross-breeding groups (F1) using Koean Israeli carp and Chinese Songpu mirror carp for the improvement of growth and scale of Israeli carp in Korea. And mating scheme for breeding groups was set in consideration of the morphological analysis and genetic distance of broodstock. In addition, this study used microsatellite markers and genotype data to analyze genetic diversity and parentage analysis. As a result, the average N_A and H_E values of Korean select broodstock are 8.3 and 0.743, and F1 is 13.0 and 0.764. This study shows that the genetic diversity of F1 has been recovered over Korean Israeli carp through breeding between Korean Israeli carp and Chinese Songpu mirror carp. Common Israeli carp in Korea reached 1.7 kg in 17 months, and improved Israeli carp reached to 2.2 kg. The KC (Korea×China, KC) group was 2.52 and broodstock group was 3.15. F1 showed lower scale score (0.63) than broodstock. The improved carp (F1; CK, KC) had 20% better scales than the parent group (F0), which improved 27% in weight and 25% in scales compared to common Israeli carp. The Israeli carp developed by the genetics-based breeding grew quicker and had improved genetic diversity and fewer scales, which will be of great value for Korean Israeli aquaculture industry due to good marketability.

• Journal of Korean Society of Forest Science
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• v.100 no.4
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• pp.577-584
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• 2011

To estimate level of genetic variation and genetic differentiation among populations of 3 populations in Abies koreana and 5 populations in Abies nephrolepis, 5 nSSR markers were analyzed. Except 1 locus where too many alleles were observed excessively, population genetic parameters were recalculated with 4 loci. Mean expected heterozygosities ($H_e$) were 0.292 in A. koreana and 0.220 in A. nephrolepis, respectively. In both species, positive fixation coefficient was estimated (F=0.065 for A. koreana and F=0.095 for A. nephrolepis), which suggests that there is an excess of homozygotes relative to Hardy-Weinberg expectations within populations. Relatively high degree of population differentiation was observed in A. koreana ($F_{ST}=0.063$). compared to that of A. nephrolepis ($F_{ST}=0.039$). From 3-level Hierarchical estimation of F-staticstics, only 4.9% of the genetic variation was allocated between species ($F_{PT}$), which suggested that most of genetic variation was shared between two species. On the basis of results from analysis of genetic relationships among populations, 2 populations of A. koreana (Mt. Halla and Mt. Deogyu) were genetically distinct from the populations of A. nephrolepis but a population of Mt. Jiri was allocated within a group of populations of A. nephrolepis. Populations of both species seemed to have undergone genetic drift due to gradual decrease in population size induced by global warming after the last glacier, which resulted in increase of homozygotes by inbreeding. It could be also postulated that these species might be diverged recently and It is likely that the two species have not fully speciated yet.

• Korean Journal of Environment and Ecology
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• v.36 no.6
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• pp.639-650
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• 2022

Abeliophyllum distichum Nakai, a rare plant with distylous characteristics, is native to certain parts of the Korean Peninsula. It is registered on the IUCN Red List of Threatened Species as a globally endangered plant. This study was conducted to establish an appropriate local conservation management plan suitable for future A. distichum populations by comparing and analyzing the flowering characteristics and population size according to distyly based on the results of quantitative surveys in 14 regions, including 8 areas with native populations of A. distichum and 6 natural monument populations. The number of individuals appearing in each population group was surveyed, and the flowering individuals were identified by style as being either pin or thrum flower types as they were being examined and recorded on the site. In total, 13,130 individuals of A. distichum (7,003 flowering and 6,127 non-flowering individuals) were recorded, but the balance of the number of pin- and thrum-flowered individuals in each population was not significant (p<0.05), indicating an imbalanced state. In particular, the Yeongdong (YD) population was very disproportionate compared to other populations, suggesting that its genetic diversity was low and the possibility of inbreeding was high. The average flowering and fruiting rates by management unit were much higher in the natural monument populations (89.2% and 55.3%, respectively) than in the natural habitat populations (39.0% and 8.5%, respectively). It may be due to a difference in reproductive growth resulting from light inflow into the forest caused by the upper crown closure. The area of occupation (AOO) of A. distichum on the Korean Peninsula covered an area of 23,224.5 m². Although the natural monument population was smaller than the natural habitat population, its density was higher, likely as a result of the periodic management of natural monument populations, where the installation of protective facilities in certain areas restricts population spread. Conservation of A. distichum populations requires removing the natural monument populations suspected of anthropogenic and genetic disturbances and expanding the conservation priority population by designating new protected areas. Although the habitats of natural monument populations are managed by the Cultural Heritage Administration and local governments, there are no agencies that are responsible for managing natural habitat populations. Therefore, institutional improvement in the overall management of A. distichum should be prioritized.