• Applied Microscopy
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• v.31 no.4
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• pp.315-323
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• 2001

In situ TEM heating experiments utilizing kaolinite powder samples result in the following facts. (1) The water recirculation system adopted in the Gatan's heating holder is required to prevent specimen drift above $500^{\circ}C$. (2) Since the degree of phase changes depends on the thickness of powders below $600^{\circ}C$, examinations of both thin and thick specimens are required. (3) Sample preparation using Mo-grids is required for TEM heating experiments above $900^{\circ}C$. At these temperature ranges the effect of heating rate and holding time on the phase transition process increases drastically, so that a programmed temperature control is required. (4) TEM heating experiments of the embedded powders by epoxy for the cross-sectional view was limited due to the severe epoxy movement during heating above $300^{\circ}C$. Better methods of sample preparation are required to overcome this problem.

• Journal of the Korean Geotechnical Society
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• v.28 no.12
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• pp.87-98
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• 2012

A sonar sensor system of a new socket roughness profiling system (SRPS) which can measure the socket roughness of the large-diameter drilled shafts under the in-situ condition was developed and verified. In model tests, the salinity, temperature, and high-turbidity have been changed for simulating the in-situ borehole water conditions. From the test results, it was found that the sonar sensor can measure the distance within an accuracy of 1mm. Because of the wave form characteristics of sonar sensor, the relative error exists in case of the inclined and curved surface, however, the shape of specimen was confirmed relatively exactly using the developed sonar sensor. Moreover, the salinity, temperature, and high-turbidity did not affect the measured data of socket roughness.

• Applied Microscopy
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• v.51
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• pp.4.1-4.12
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• 2021

Fluorescence in situ hybridization (FISH) is a technique to visualize specific DNA/RNA sequences within the cell nuclei and provide the presence, location and structural integrity of genes on chromosomes. A confocal Whole Slide Imaging (WSI) scanner technology has superior depth resolution compared to wide-field fluorescence imaging. Confocal WSI has the ability to perform serial optical sections with specimen imaging, which is critical for 3D tissue reconstruction for volumetric spatial analysis. The standard clinical manual scoring for FISH is labor-intensive, time-consuming and subjective. Application of multi-gene FISH analysis alongside 3D imaging, significantly increase the level of complexity required for an accurate 3D analysis. Therefore, the purpose of this study is to establish automated 3D FISH scoring for z-stack images from confocal WSI scanner. The algorithm and the application we developed, SHIMARIS PAFQ, successfully employs 3D calculations for clear individual cell nuclei segmentation, gene signals detection and distribution of break-apart probes signal patterns, including standard break-apart, and variant patterns due to truncation, and deletion, etc. The analysis was accurate and precise when compared with ground truth clinical manual counting and scoring reported in ten lymphoma and solid tumors cases. The algorithm and the application we developed, SHIMARIS PAFQ, is objective and more efficient than the conventional procedure. It enables the automated counting of more nuclei, precisely detecting additional abnormal signal variations in nuclei patterns and analyzes gigabyte multi-layer stacking imaging data of tissue samples from patients. Currently, we are developing a deep learning algorithm for automated tumor area detection to be integrated with SHIMARIS PAFQ.

• Korean Journal of Materials Research
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• v.25 no.12
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• pp.690-693
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• 2015

Fatigue crack growth experiments were carried out on a 304 L stainless steel compact-tension(CT) specimen under load control mode. Neutron diffraction was employed to quantitatively measure the residual strains/stresses and the evolution of stress fields in the vicinity of a propagating fatigue-crack tip. Three principal stress components (i.e. crack growth, crack opening, and through-thickness direction stresses) were examined in-situ under loading as a function of distance from the crack tip along the crack-propagation path. The stress/strain fields, measured both at the mid-thickness and near the surface of the CT specimen, were compared. The results show that much higher compressive residual stress fields developed in front of the crack tip near the surface than developed at the mid-thickness area. The change of the stresses ahead of the crack tip under loading is more significant at the mid-thickness area than it is near the surface.

• Transactions of the Korean Society of Automotive Engineers
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• v.13 no.5
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• pp.171-180
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• 2005

Bending performances of aluminum square tube beams reinforced by aluminum plates under three point bending loads have been evaluated using experimental tests combined with theoretical and finite element analyses. A finite element simulation for the three-point bending test was performed. Basic properties of aluminum materials used for initial input data of the finite element simulation were obtained from the true stress-true strain curves of specimens which had been extracted from the Al tube beams. True stresses were determined from applied loads and cross-sectional area records of a tensile specimen with a rectangular cross-section by real-time photographing, and true strains were obtained from in-situ local elongation measurements of the specimen gage portion by the multi-point scanning laser extensometer. Six kinds of aluminum tube beam specimens adhered by aluminum plates were employed fur the bending test. The bending deformation behaviors up to the maximum load described by the numerical simulation were in good agreement with experimental ones. After passing the maximum load, reinforcing plate was debonded from the aluminum tube beam. An aluminum tube beam strengthened by aluminum plate on the upper web showed an excellent bending capability.

• Tunnel and Underground Space
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• v.8 no.3
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• pp.226-233
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• 1998

Microcracks in rock materials, whether natural or induced, provide useful information on the engineering performance of in situ rockmasses. A population of preferentially oriented microcracks has observable effects on the physical properties of a rockmass, but their effects may not be evident if the rock material is highly anisotropic due to other causes. An experimental program was undertaken to investigate the effect of rock fabrics on the physical properties of rock materials. In this study, anisotropy in the circumferential wave velocity and the direction of induced fractures under axial point loading were measured. Rock specimens (NX-size) of the leucocractic Pocheon granite were cored from rock blocks, retaining the relative directions of each specimen. Another set of specimens was prepared from the rock cores of the same meterial, obtained in the field. The master orientation line (MOL) was set to be the representative direction of the microcracks in the specimen. Variation of the circumferential wave velocity of each specimen was then measured along the core, keeping the MOL as reference. The direction of the minimum wave velocity was nearly perpendicular to the direction of the MOL. Coring of smaller-sized (EX-size), concentric specimens from the NX specimens were then followed, and axial point loading was applied. The direction of induced fractures due to axial point loading was closely related to the MOL direction, confirming the prior test result.

• Proceedings of the KSME Conference
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• 2007.05a
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• pp.60-65
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• 2007

Local buckling behaviors of aluminum square tube beams reinforced by aluminum plates under three point bending loads have been analyzed using experimental tests combined with theoretical and finite element analyses. For this analysis true stresses were determined from applied loads and cross-sectional area records of a tensile specimen with a rectangular cross-section by real-time photographing. True strains were also obtained from in-situ local elongation measurements of the specimen gage portion by the multi-point scanning laser extensometer. Six kinds of aluminum tube beam specimens reinforced by aluminum plates were employed for the bending test. The bending deformation behaviors up to the maximum load analyzed by the numerical simulation agreed well with experimental ones. After passing the maximum load, reinforcing plate hindering the local buckling of the tube beam was debonded from the aluminum tube beam. An aluminum tube beam strengthened by aluminum plate on the upper web showed the most excellent bending capacity, which could be explained on the basis of the neutral axis shift and the local buckling deformation range.

• Journal of the Korean Ceramic Society
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• v.31 no.10
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• pp.1099-1106
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• 1994

It is suggested that the microstructural toughening process in the initial rising portion of R-curves observed in polycrystalline alumina should be different from the grain bridging mechanism identified in the long crack regime. Microcracking in the advancing crack front seems to be a prerequisite for the development of unbroken bridging ligaments behind the crack tip. In order to test such a proposition, attempts were made to identify experimentally the presence of microcracks in the frontal zone of propagating cracks. In-situ observation is made of crack growth in a miniature double cantilever beam specimen of a average grain size of 10 ${\mu}{\textrm}{m}$ alumina. Presence of a few microcracks was identified in front of crack tip on the propagating crack plane. The R-curves were re-evaluated based on the observation.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.04a
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• pp.183-185
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• 2004

In this study, the combined electrokinetic and ultrasonic remediation technique, ultrasonically enhanced electrokinetic technique, was studied for the removal of heavy metal and organic substance in contaminated soils. The electrokinetic technique has been applied to remove mainly the heavy metal and the ultrasonic technique has been to remove mainly organic substance in contaminated soil. The laboratory soil flushing tests combined electrokinetic and ultrasonic technique were conducted using specially designed and fabricated devices to determine the effect of these both techniques. A series of laboratory experiments involving the simple, electrokinetic, ultrasonic, and electrokinetic & ultrasonic flushing test were carried out. A soil admixed with sand and kaolin was used as a test specimen, and Pb and ethylene glycol were used as contaminants of heavy metal and organic substance. An increase in out flow, permeability and contaminant removal rate was observed in electrokinetic and ultrasonic flushing tests. Some practical implications of these results are discussed in terms of technical feasibility of in situ implementation of electrokinetic ultrasonic remediation technique.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2004.10a
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• pp.429-436
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• 2004

For the in-situ health monitoring of critical members in civil infra-structures, ultrasonic guided Lamb waves-based non-destructive evaluation (NDE) is very suitable. However, a chief drawback of the Lamb wave techniques is that multiple modes exist at all frequencies and the modes are generally dispersive, which means that the received signals may be very complicated. To overcome these complications, selective transmitting and receiving of a single A/sub 0/ mode within a frequency range can be adopted. Furthermore, a wavelet technique can be utilized to decompose the Lamb wave response into wavelet coefficients as a tool for signal processing. The changes in the Lamb waves interacting with damages in the steel plates are successfully characterized by this wavelet technique, through the amplitude change of the wavelet coefficients. In this paper, the feasibility of detecting a line crack on the surface of a steel plate and loosened bolts in a joint steel specimen using the Lamb waves and the wavelet technique is investigated.