• Title/Summary/Keyword: In-situ mixing

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Highly Sensitive Biological Analysis Using Optical Microfluidic Sensor

  • Lee, Sang-Yeop;Chen, Ling-Xin;Choo, Jae-Bum;Lee, Eun-Kyu;Lee, Sang-Hoon
    • Journal of the Optical Society of Korea
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    • v.10 no.3
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    • pp.130-142
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    • 2006
  • Lab-on-a-chip technology is attracting great interest because the miniaturization of reaction systems offers practical advantages over classical bench-top chemical systems. Rapid mixing of the fluids flowing through a microchannel is very important for various applications of microfluidic systems. In addition, highly sensitive on-chip detection techniques are essential for the in situ monitoring of chemical reactions because the detection volume in a channel is extremely small. Recently, a confocal surface enhanced Raman spectroscopic (SERS) technique, for the highly sensitive biological analysis in a microfluidic sensor, has been developed in our research group. Here, a highly precise quantitative measurement can be obtained if continuous flow and homogeneous mixing condition between analytes and silver nano-colloids are maintained. Recently, we also reported a new analytical method of DNA hybridization involving a PDMS microfluidic sensor using fluorescence energy transfer (FRET). This method overcomes many of the drawbacks of microarray chips, such as long hybridization times and inconvenient immobilization procedures. In this paper, our recent applications of the confocal Raman/fluorescence microscopic technology to a highly sensitive lab-on-a-chip detection will be reviewed.

Preparation and Properties of EPDM/Zinc Methacrylate Hybrid Composites (에틸렌 프로필렌 디엔 고무/메타크릴산아연 하이브리드 복합체의 제조와 물성에 관한 연구)

  • Chang, Young-Wook;Won, Jong-Hoon;Joo, Hyun-Seok
    • Elastomers and Composites
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    • v.40 no.1
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    • pp.59-65
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    • 2005
  • Zinc methacrylate(ZMA) was incorporated into ethylene-propylene diene rubber(EPDM) by direct mixing of the metal salt with the rubber or was in-situ prepared in the rubber matrix through neutralization reaction of zinc oxide(ZnO) and methacrylic acid(MAA). Tensile and tear tests showed that ZMA had a great reinforcing effect for the EPDM. It was also found that ZMA reinforced EPDM vulcanizates can retain their mechanical properties under thermo-oxidative aging. Moreover the incorporation of ZMA induces a substantial improvement in the adhesive strength of the EPDM onto aluminum substrate. The reinforcing effect and an enhancement in adhesion was greatly manifested when the ZMA is in-situ formed with an excess amount of ZnO. The extraordinary improvement in the properties is supposed to be related with the formation of ionic crosslink as well as the degree of dispersion or ZMA domain in the rubber matrix.

Engineering Properties of Liquefied Stabilized Soil by Contents of Humic Acid (휴믹산 함유량에 따른 유동화 처리토의 공학적 특성)

  • Han, Sang-Jae;Ahn, Dong-Wook;Park, Jea-Man;Kim, Soo-Sam
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.5C
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    • pp.229-237
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    • 2009
  • A conventional way of backfilling has used sand or in-situ soil. It not only requires substantial amount of time and cost but also makes it particularly difficult to fill the bottom part and small cracks of a pipe. To address the problem with the conventional method of compaction, liquefied stabilized soil was proposed as an alternative because it reuses in-situ soil which can ensure sand supply while adjusting flowability and strength of the soil with design of mix proportion. With an aim to identify the mixing properties of liquefied stabilized soil depending on the organic content of in-situ soil, this study conducted indoor tests of material segregation, flowability, strength, and permeability by changing humic acid content of the soil. The results revealed that material segregation and flowability increased proportionally while strength decreased with the increased amount of humic acid. In the mean time, permeability of liquefied stabilized soil wasn't affected by organic content.

Effects of Mixing Time for Total Mixed Rations using Corn Silage on Ruminal In situ Dry Matter Degradation and Milk Production in Dairy Cows (옥수수 사일리지 이용 섬유질배합사료의 배합시간에 따른 In situ 반추위 분해율 및 착유우의 산유특성에 미치는 영향)

  • Lim, Dong-Hyun;Ki, Kwang-Seok;Choi, Sun-Ho;Kim, Tae-Il;Park, Seong-Min;Park, Su-Bum;Kwon, Eung-Gi;Kim, Eun-Tae
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.34 no.4
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    • pp.288-295
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    • 2014
  • This study was conducted to evaluate the effect of mixing time for TMR (total mixed rations) mixed with corn silage on particle size, peNDF (physically effective neutral detergent fiber), laceration, and ruminal in situ dry matter degradation. The study also aimed to evaluate the effect of consumption of the TMR on the milk yield and milk components of mid-lactational dairy cows. TMRs were mixed for 30 minutes (T1) and 50 minutes (T2) using the same material. All samples were then analyzed with a Penn State Particle Size Separator (PSPS). The particle size of T1 was significantly lower in the bottom pan (8 mm>) than that of T2 (p<0.01). $peNDF_{&gt;8.0}$ was significantly higher in T1 (17.18%) than in T2 (13.85%) (p<0.01). For ruminal in situ dry matter degradation of particle retention (>19 mm), no significant difference was found after 72 hours incubation, although T1 degradation was significantly higher after 24 hours incubation (p<0.01). Milk yield was no different between the groups of cows, whereas the milk fat from T1 fed cows was significantly higher (p<0.01). The results show that feeding TMR mixed for 30 minutes to dairy cows may improve the physical value of forage without negative effects on the milk yield and milk components.

Spatial Variability of in situ and GOCI and MODIS Chlorophyll and CDOM in Summer at the East Sea (여름철 동해의 현장측정치와 GOCI와 MODIS 위성 자료로 측정한 엽록소와 유색용존유기물의 공간 변동성)

  • Park, Mi-Ok;Shin, Woo-Chul;Son, Young-Baek;Noh, Tae-Geun
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.21 no.4
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    • pp.327-338
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    • 2015
  • Because of impact on the underwater light field, CDOM can influence the accuracy of global satellite-based measurement of ocean chlorophyll and primary productivity. So we investigated the distribution and seasonal variation of CDOM in the East Sea during summer 2009 and 2011. Among them we report two distinctively different summer cases between 2009 and 2011 year, in which showed the different main sources for CDOM. Regulating factors and sources of CDOM in the East Sea were examined. Comparison between in situ and satellite derived Chl a and CDOM were made to find an influence of CDOM on measurement of satellite derived Chl a. Similar pattern and matching of MODIS Chl a with in situ Chl a 2009 was comparable, but significant discrepancy between MODIS Chl a and in situ Chl a was found, when CDOM was high in summer of 2011. GOCI data showed better matching with in situ data for both Chl a and CDOM, compared to MODIS data in summer of 2011. The presence of high CDOM at the surface layer supplied by vertical mixing seems to affect on the overestimation of Chl a by satellite data.

Distribution of Water Masses and Characteristics of Temperature Inversion in the Western Seas of Jeju Island in Spring (봄철 제주도 서부해역의 수괴 분포와 수온역전 특징)

  • Kang, So-Young;Moon, Jae-Hong
    • Ocean and Polar Research
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    • v.44 no.3
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    • pp.191-207
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    • 2022
  • Using the results of CTD casts made in Spring from 2017 to 2021, in this study we investigated the water mass distribution and occurrence of temperature inversion in the western seas of Jeju Island in spring. The distribution of water masses was characterized by cold and fresh water in the northwest and warm and saline water in the southeast, forming a strong thermohaline front running in the southwest-to-northeast direction. Strong temperature inversion mainly occurred in the frontal boundary when the cold water intrudes beneath the warm water at depths of 30-50 m. Analysis of the mixing ratio demonstrated that Jeju Warm Water is dominantly distributed in the western seas of Jeju Island, but its ratio can be modified depending on the southward extension of Yellow Sea Cold Water (YSCW). Results of in situ measurement showed that in 2020, the YSCW largely expanded to the western seas of Jeju Island, occupying approximately 40 % of the mixing ratio. Due to the expansion of YSCW, a strong thermohaline front was formed in the study area, thereby causing thick and strong temperature inversion. On the other hand, in 2018 the mixing ratio of YSCW was minimum (~18%) during the study period of 2017-2021, and thus a relatively weak frontal boundary was formed, without the occurrence of temperature inversion. The observational results also suggest that the interannual changes of water mass distribution and the associated temperature inversion in the western seas of Jeju Island are closely related with wind-driven Yellow Sea circulation in spring, which is the summer monsoon transition period.

Fundamental Study for Feasibility on Soil Flushing for TPHs-Contaminated Soil Treatment (유류오염토양 정화를 위한 토양세정기술의 적용성 기초연구)

  • Kang, Hui-Cheon;Kim, Joung-Dae;Han, Byeong-Gi;Seo, Seung-Won;Shin, Chul-Ho;Park, Joon-Seok
    • Journal of the Korean Applied Science and Technology
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    • v.32 no.4
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    • pp.740-747
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    • 2015
  • This research was performed to evaluate the feasibility of in situ soil flushing for TPH-contaminated soil remediation. It was conducted in batch test as fundamental research for in situ soil flushing. The 30% of initial TPH concentration was removed by shaking only in batch test. The removal efficiency of TPH in case of groundwater as surfactant dilution solution was approximate 2~6% lower than that of distilled water. Mixing ratio of soil to surfactant solution did not practically effect on the TPH removal efficiency. In the experiment of using single or mixed surfactant solution with 0.1~4.0 wt%, Tween-80, SWA-1503, SWA-1503+SDS showed averagely over 80%. It was determined that the optimum surfactant concentration was 0.1 wt% because there was no significant difference between concentrations of 0.1~4.0 wt%.

Remote Sensing of NO2 vertical profiles in Beijing on the basis of MAX-DOAS measurements (지상 MAX-DOAS를 이용한 중국 베이징에서의 이산화질소 연직분포 산출 및 지상자료와 비교를 통한 검증연구)

  • Ryu, Jae-Yong
    • Korean Journal of Remote Sensing
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    • v.30 no.6
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    • pp.703-708
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    • 2014
  • $NO_2$ vertical profiles were retrieved via ground based MAX-DOAS measurements for the summer period in 2006 in Beijing, one of the megacities in the Northeast Asia. Large portion of $NO_2$ load was observed at the 0-1 km layer. We found that $NO_2$ rapidly decreases up to the altitude of 3 km. In addition, the retrieved $NO_2$ mixing ratios within 0-1 km layer were compared with those observed at the surface by in-situ monitor. The correlation coefficient (R) between $NO_2$mixing ratios within 0-1 km layer and those at the surface was calculated to be 0.7. The major causes of such discrepancy are thought to be both differences in measured areas and rapid decrease in $NO_2$ mixing ratio with height.

The Functionalization and Preparation Methods of Carbon Nanotube-Polymer Composites: A Review (탄소나노튜브-폴리머 복합체의 기능화와 제조방법)

  • Oh, Won-Chun;Ko, Weon-Bae;Zhang, Feng-Jun
    • Elastomers and Composites
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    • v.45 no.2
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    • pp.80-86
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    • 2010
  • Carbon nanotubes (CNTs) exhibit excellent mechanical, electrical, and magnetic properties as well as nanometer scale diameter and high aspect ratio, which make them an ideal reinforcing agent for high strength polymer composites. The functionalized CNTs are believed to be very promising in the fields such as preparation of functional and composite materials. CNT-Polymer composites are expected to have good processability characteristics of the polymer and excellent functional properties of the CNTs. However, since CNTs usually form stabilized bundles due to Van der Waals interactions, are extremely difficult to disperse and align in a polymer matrix. The biggest issues in the preparation of CNT-reinforced composites reside in efficient dispersion of CNTs into a polymer matrix, and the alignment and control of the CNTs in the matrix. There are several methods for the dispersion of nanotubes in the polymer matrix such as solution mixing, bulk mixing, melt mixing, in-situ polymerization and chemical functionalization of the carbon nanotubes, etc. These methods and preparation of high performance CNT-polymer composites are described in this review.

Research on manufacturing secondary construction products using in-situ carbonation technology (In-situ 탄산화 기술이 적용된 콘크리트 2차제품 제조 연구)

  • Hye-Jin Yu;Sung-Kwan Seo;Woo-Sung Yum
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.33 no.6
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    • pp.226-233
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    • 2023
  • In this study, the basic physical properties and microstructure of concrete interlocking blocks with amount of different CO2 gas injection were analyzed according to determine the applicability of In-situ carbonation technology to construction secondary products. The amount of carbon dioxide gas injection was selected as 0, 0.1, 0.3, 0.5, 0.7 wt.% compared to cement amount. A lab-scale press equipment was designed to apply developed carbonation technology to real construction site. And mixer for stable CO2 gas injection was designed. Using the designed devices, CO2 gas injected samples were created and physical property of samples were performed. As a result of the physical property test, as the CO2 injection amount increased to 0.3 %, it showed higher strength behavior compared to the original mix. And more than 0.5 % samples showed lower strength behavior than original sample, but they satisfied the standard of concrete interlocking block. This results were determined that CO2 injection contributed to the creation of hydrates such as C-S-H. Therefore, the possibility of applying carbonation technology, which injects CO2 during mixing, to various secondary construction products was confirmed.