• Asian-Australasian Journal of Animal Sciences
• /
• v.20 no.9
• /
• pp.1397-1401
• /
• 2007

An in situ experiment was conducted to find out whether Tween 80 improves rice straw digestion through increased adhesion of major fibrolytic bacteria. Rice straw was sprayed with various levels of Tween 80 non-ionic surfactant or SDS ionic surfactant 24 h before incubation in the rumen of Holstein steers. Dry matter (DM) disappearance and adhesion of F. succinogenes, R. flavefaciens and R. albus on rice straw after in situ incubation were measured by real-time PCR. Application of Tween 80 increased DM disappearance, which was more noticeable at an application level of 1% compared to lower application levels. Application of SDS resulted in an opposite response in DM disappearance with highest reduction in DM disappearance at 1% level. In a subsequent in situ experiment, higher Tween 80 was applied to rice straw in an attempt to find the optimum application level. Tween 80 at 2.5% gave better DM disappearance than 1% with a similar result at 5%. Therefore, an adhesion study was carried out using rice straw treated with 2.5% Tween 80. Our results indicated that Tween 80 reduced adhesion of all three major rumen fibrolytic bacteria to rice straw. Present data clearly show that improved DM disappearance by Tween 80 is not due to increased bacterial adhesion onto substrates.

• Journal of the Korean Professional Engineers Association
• /
• v.41 no.3
• /
• pp.35-39
• /
• 2008

The most common soil contaminants are petroleum-based. Hydrocarbons from diesel fuel and gasoline are widespread problems, as are total petroleum hydrocarbon(TPH). There are two distinct classes of soil remediation: in-situ, or on-site, and ex-situ, or off- site. On-site cleanups are often preferred because they are cheaper. On the other hand, excavating a contaminated area and transporting it to a remote site before cleaning it can often be more complete. Ex-situ remediation also has the added bonus of taking the bulk of contaminants off-site before they can spread further. In addition, in-situ situations are limited because only the topside of the soil is accessible.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.13 no.1 s.53
• /
• pp.152-160
• /
• 2009

The pipe jacking method which is a non-excavation method is frequently used due to constructability and economical efficiency in a medium or small-sized pipeline construction. However, jacking process of the method still causes problems that the base ground is disturbed and loosen. These lead to surface settlement, strength decrease and leakage of water. Therefore, this study presents in-situ application of the steel pipe jacking with grouting, and it is that jacking and grouting are progressed simultaneously. To verify this, the steel pipe jacking with grouting and the existing steel pipe jacking have been constructed on the same ground condition. It has been proved that the steel pipe jacking with grouting is in-situ applicable according to results of monitoring surface settlement, in-situ density, GPR geophysical prospecting and large scale direct shear test.

• Journal of the Korean Geotechnical Society
• /
• v.18 no.2
• /
• pp.65-74
• /
• 2002

The measurement of in-situ stress is essential to estimate the ground displacement and the stress distribution of a tunnel and an underground structure. In this study, the in-situ stress distribution of the Southern Korean peninsula was re-evaluated by the new 380 in-situ data which were determined by overcoring and hydrofracturing methods, and the three-din erosional numerical analysis of tunnelling was performed. The results of in-situ stress distribution show that the distribution of horizontal stress tends to be more irregular in metamorphosed(gneiss) and granite areas than in sedimentary and volcanic areas. The ratio of horizontal to vertical stresses(K-value) in volcanic area is less than 1 below the depth of 150m. The direction and magnitude of three dimensional in-situ stresses were shown simultaneously in a figure for the first time in Korea. The three-dimensional numerical analysis of tunnelling indicates that the orientation and magnitude of displacement around a tunnel are controlled mainly by the difference between the maximum and minimum horizontal stresses.

• The Korean Journal of Cytopathology
• /
• v.7 no.1
• /
• pp.38-43
• /
• 1996

Epstein-Barr virus(EBV) is associated with a wide spectrum of benign and malignant disorders including leukoplakia, Hodgkln's lymphoma, central nervous system lymphoma, peripheral T cell lymphoma and nasopharyngeal undifferentiated carcinoma. There are several distinctive aspects of biology of the virus that are important in investigation of virus in clinical specimens. The abundant expression of the EBER mRNA transcripts makes possible the sensitive detection of latent expression in EBV-associated tumors. Although there has been a dramatic increased interest in the direct characterization of EBV in clinical specimens, there have been few studios about the effective and reliable positive controls in performing in situ hybridization technique for EBV, especially on paraffin-em bedded tissue. We applied Burkitts lymphoma ceil line as positive control in EBV in situ hydridization using Oncor Kit. The cell block of Burkitt lymphoma cell line(CCL85 EB-3) showed strong and specific positivity for EBER in situ in nuclei of EBV infected cells.

• International Journal of Highway Engineering
• /
• v.17 no.3
• /
• pp.85-95
• /
• 2015

PURPOSES : Recently, bonded concrete overlay has been used as an alternative solution in concrete pavement rehabilitation since its material properties are similar to those of the existing concrete pavements. Deteriorated concrete pavements need rapid rehabilitation in order to prevent traffic jams on Korean expressways. Moreover, speedy and effective repair methods are required. Therefore, the use of bonded concrete overlay with ultra-rapid hardening cement has increased in an effort to reopen promptly the expressways in Korea. However, mobile mixer is required for ultra-rapid hardening cement concrete mixing in the construction site. The use of mobile mixer causes various disadvantages aforementioned such as limitation of the construction supply, open-air storage of mixing materials, increase in construction cost, and etc. In this study, therefore, hydration accelerator in-situ mixing on polymer modified concrete produced in concrete plant is attempted in order to avoid the disadvantages of existing bonded concrete overlay method using ultra-rapid hardening cement. METHODS : Bonded concrete overlay materials using ultra-rapid hardening cement should be meet all the requirements including structural characteristics, compatibility, durability for field application. Therefore, This study aimed to evaluate the application of hydration accelerator in-situ mixing on polymer modified concrete by evaluating structural characteristics, compatibility, durability and economic efficiency for bonded concrete overlay. RESULTS : Test results of structural characteristics showed that the compressive, flexural strength and bond strength were exceed 21MPa, 3.15MPa and 1.4MPa, respectively, which are the target strengths of four hours age for the purpose of prompt traffic reopening. In addition, tests of compatibility, such as drying shrinkage, coefficient of thermal expansion and modulus of elasticity, and durability (chloride ions penetration resistance, freezing-thawing resistance, scaling resistance, abrasion resistance and crack resistance), showed that the hydration accelerator in-situ mixing on polymer modified concrete were satisfied the required criteria. CONCLUSIONS : It was known that the hydration accelerator in-situ mixing on polymer modified concrete overlay method was applicable for bonded concrete overlay and was a good alternative method to substitute the existing bonded concrete overlay method since structural characteristics, compatibility, durability were satisfied the criteria and its economic efficiency was excellent compare to the existing bonded concrete overlay methods.

• Fashion & Textile Research Journal
• /
• v.22 no.3
• /
• pp.386-398
• /
• 2020

This study compared dip-coating and in situ polymerization methods for the development of nanofiber-based E-textile using polypyrrole. Nanofiber webs were fabricated by electrospinning an aqueous poly (vinyl alcohol) (PVA) solution. Subsequently, the PVA nanofiber web underwent thermal treatment to improve water resistance. Dip-coating and in situ polymerization methods were used to deposit polypyrrole on the surfaces of the nanofiber web. An FE-SEM analysis was also conducted to examine specimen surface characteristics along with EDS and FT-IR that analyzed the chemical bonding between polypyrrole and specimens. The line resistance and sheet resistance of the treated specimens were measured. Finally, an electrocardiogram (ECG) was measured with textile sensors made of the polypyrrole-deposited PVA nanofiber webs. The polypyrrole-deposited PVA nanofiber webs fabricated by dip-coating dissolved in the dip-coating solution and indicated damage to the nanofibers. However, in the case of in situ polymerization, polypyrrole nanoparticles were deposited on the surface and inter-web structure of the PVA nanofiber web. The resistance measurements indicated that polypyrrole-deposited PVA nanofiber webs fabricated by in situ polymerization with an average sheet resistance of 5.3 k(Ω/□). Polypyrrole-deposited PVA nanofiber webs fabricated by dip-coating showed an average sheet resistance of 57.3 k(Ω/□). Polypyrrole-deposited PVA nanofibers fabricated by in situ polymerization showed a lower line and sheet resistance; in addition, they detected the electrical activity of the heart during ECG measurements. The electrodes made from polypyrrole-deposited PVA nanofiber webs by in situ polymerization showed the best performance for sensing ECG signals among the evaluated specimens.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2000.03a
• /
• pp.65-84
• /
• 2000

Europe and US which have more restrictive regulations than Korea about the noise and vibration during construction are using Auger-cast Pile to reduce the problem relating with noise and vibration. However Auger-cast Pile has problems like difficult quality control and low bearing capacity. In Europe, Displacement in-situ concrete Pile has been used to sove that problems since 1990s, and Korea has performed the test construction in 1997 and it has been used as the real structural foundation since 1998. Test and real construction results verified that the allowable capacity of the pile(diameter = 410mm) is between 70 and 100ton. Though De Beer & Van Imps design method utilizing CPT result is used to calculate the bearing capacity of the Displacement in-situ Pile, Korea is dependant upon the SPT as the sounding test, so design method utilizing SPT result is necessary to promote the application of the pile. To find out reasonable design method using SPT result, rearing capacity of the pile constructed in sand and clay in Korea was calculated using Meyerhof, SPT-CPT translation method, Nordlund, Douglas and DM-7 method, and the calculation results were compared to the load test result. Analysis result shows that SPT-CPT translation method is more reliable than others and economical design can be possible because it considers efficiently the friction capacity of Displacement in-situ Pile.

• Journal of Korean Society on Water Environment
• /
• v.29 no.5
• /
• pp.691-702
• /
• 2013

This review paper summarizes the theory, application, and potential drawbacks of diffusive gradient in thin film (DGT) probe which is a widely used in-situ passive sampling technique for monitoring inorganic contaminants in aquatic environments. The DGT probe employs a series of layers including a filter membrane, a diffusive hydrogel, and an ionic exchange resin gel in a plastic unit. The filter side is exposed to an aquatic environment after which dissolved inorganic contaminants, such as heavy metals and nuclides, diffuse through the hydrogel and are accumulated in the resin gel. After retrieval, the contaminants in the resin gel are extracted by strong acid or base and the concentrations are determined by analytical instruments. Then aqueous concentrations of the inorganic contaminants can be estimated from a mathematical equation. The DGT has also been used to monitor nutrients, such as ${PO_4}^{3-}$, in lakes, streams, and estuaries, which might be helpful in assessing eutrophic potential in aquatic environments. DGT is a robust in-situ passive sampling techniques for investigating bioavailability, toxicity, and speciation of inorganic contaminants in aquatic environments, and can be an effective monitoring tool for risk assessment.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
• /
• 2003.10a
• /
• pp.89-110
• /
• 2003

The limitations of conventional soil and groundwater contamination remediation technologies have motivated a search for innovative technologies; particularly in situ technologies that do not require extraction of contaminants from the subsurface. All engineered in situ remediation systems require that the contaminant be mixed with a remedial compound. Horizontal flow treatment wells (HFTWs), an innovative technology that consists of a pair of dual-screened treatment wells, were used at a trichloroethylene (TCE) contaminated site to efficiently achieve this mixing of contaminant and remedial compound in order to effect in situ bioremediation (McCarty et al., 1998). In this paper, the potential of HFTWs to treat chlorinated aliphatic hydrocarbons (CAHs) as well as other soil and groundwater contaminants of concern, such as nitroaromatic compounds (NACs), perchlorate, and methyl-tert-butyl ether (MTBE), is examined. Through a combination of laboratory studies, model analyses, and field evaluations, the effectiveness of this innovative technology to manage these contaminants is investigated.