The Korean Journal of Cytopathology (대한세포병리학회지)

The Korean Society for Cytopathology (대한세포병리학회)
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Application of Epstein-Barr Virus Cell Lines (CCL85 EB-3) in Performing the EBER mRNA In Situ Hybridization as a Positive Control

Epstein-Barr 바이러스 인사이투 보결합 시행시 양성대조표지로서의 버키트 림프종 세포주 (CCL85 EB-3)의 응용

  • Kim, Sung-Sook (Departments of Pathology, Ewha Womans University, School of Medicine) ;
  • Han, Woon-Sup (Departments of Pathology, Ewha Womans University, School of Medicine) ;
  • Suh, Joo-Young (Departments of Microbioloy, Ewha Womans University, School of Medicine) ;
  • Huh, Joo-Ryung (Department of Pathology, Asan Medical Center, Ulsan University)
  • 김성숙 (이화여자대학교 의과대학 병리학교실) ;
  • 한운섭 (이화여자대학교 의과대학 병리학교실) ;
  • 서주영 (이화여자대학교 의과대학 미생물학교실) ;
  • 허주령 (울산의대부속 서울중앙병원 해부병리과)
  • Published : 1996.06.29
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Abstract

Epstein-Barr virus(EBV) is associated with a wide spectrum of benign and malignant disorders including leukoplakia, Hodgkln's lymphoma, central nervous system lymphoma, peripheral T cell lymphoma and nasopharyngeal undifferentiated carcinoma. There are several distinctive aspects of biology of the virus that are important in investigation of virus in clinical specimens. The abundant expression of the EBER mRNA transcripts makes possible the sensitive detection of latent expression in EBV-associated tumors. Although there has been a dramatic increased interest in the direct characterization of EBV in clinical specimens, there have been few studios about the effective and reliable positive controls in performing in situ hybridization technique for EBV, especially on paraffin-em bedded tissue. We applied Burkitts lymphoma ceil line as positive control in EBV in situ hydridization using Oncor Kit. The cell block of Burkitt lymphoma cell line(CCL85 EB-3) showed strong and specific positivity for EBER in situ in nuclei of EBV infected cells.

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