• BMB Reports
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• v.52 no.11
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• pp.659-664
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• 2019

Vav1 is a Rho/Rac guanine nucleotide exchange factor primarily expressed in hematopoietic cells. In this study, we investigated the potential role of Vav1 in osteoclast (OC) differentiation by comparing the ability of bone marrow mononuclear cells (BMMCs) obtained from Vav1-deficient ($Vav1^{-/-}$) and wild-type (WT) mice to differentiate into mature OCs upon stimulation with macrophage colony stimulating factor and receptor activator of nuclear kappa B ligand in vitro. Our results suggested that Vav1 deficiency promoted the differentiation of BMMCs into OCs, as indicated by the increased expression of tartrate-resistant acid phosphatase, cathepsin K, and calcitonin receptor. Therefore, Vav1 may play a negative role in OC differentiation. This hypothesis was supported by the observation of more OCs in the femurs of $Vav1^{-/-}$ mice than in WT mice. Furthermore, the bone status of $Vav1^{-/-}$ mice was analyzed in situ and the femurs of $Vav1^{-/-}$ mice appeared abnormal, with poor bone density and fewer number of trabeculae. In addition, Vav1-deficient OCs showed stronger adhesion to vitronectin, an ${\alpha}_v{\beta}_3$ integrin ligand important in bone resorption. Thus, Vav1 may inhibit OC differentiation and protect against bone resorption.

• Journal of the Korea Society of Computer and Information
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• v.16 no.11
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• pp.35-44
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• 2011

Advances in science and technology have made a lot of changes in our life. Especially, sensors have used in various ways to monitor in real time and analyze the world effectively. Traditional sensor networks, however, have used their own protocols and architecture so it had to be paid a lot of additional cost. In the past 8 years, OGC and ISO have been formulating standards and protocols for the geospatial Sensor Web. Although the OGC SWE initiatives have deployed some components, attempts have been made to access sensor data. All spatial operations had to calculate on the client side because traditional SOS architecture did not consider spatial operation for GeoSensor. As a result, clients have to implement and run spatial operations, and it caused a lot of overload on them and decreased approachableness. In this paper we propose S-SOS for in-situ and moving GeoSensor that extends 52 North SOS and provides spatialFilter and spatialFinder operations. The proposed S-SOS provides an architecture that does not need to edit already deployed SOSs and can add spatial operations as occasion. Additionally we explain how to express the spatial queries and to be used effectively for various location based services.

• Journal of the Korean Society of Marine Environment & Safety
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• v.20 no.6
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• pp.601-608
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• 2014

The purpose of this study was to explore the long-term variability of sea surface temperature (SST) and cluster analysis derived from in-situ data in the coastal oceanographic observation 8 stations (Sokcho, Jumunjin, Donghae, Jukbyeon, Pohang, Janggigab, Ulgi, Gampo) of the East Sea during 1971-2013. As a result of cluster analysis, SST variations in each area could be divided into two groups, which was a group A of Sokcho, Jumunjin, Donghae, and group B of Jukbyeon, Pohang, Janggigab, Ulgi, Gampo. The SST and SST anomalies at Sokcho, Jukbyeon, Pohang and Gampo during 1971-2013 showed the increase-trend with the variations of decadal-scale. Annual SST values also increased remarkably after 1988. The increases of SST for 43 years showed $2.26^{\circ}C$ at Sokcho, $1.99^{\circ}C$ at Jukbyeon, $1.11^{\circ}C$ at Pohang and $0.89^{\circ}C$ at Gampo. In particular, the SST variations of the northern areas were higher than those of southern areas. The seasonal SSTs increased the order of Sokcho-Jukbyeon-Pohang-Gampo in fall and winter and that of Jukbyeon-Sokcho-Pohang-Gampo in spring and summer.

• International Journal of Automotive Technology
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• v.7 no.4
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• pp.391-397
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• 2006

The soot yield has been studied by a premixed propane-oxygen-inert gas combustion in a specially designed disk-type constant-volume combustion chamber to investigate the effect of pressure, temperature and turbulence on soot formation. Premixtures are simultaneously ignited by eight spark plugs located on the circumference of chamber at 45 degrees intervals in order to observe the soot formation under high temperature and high pressure. The eight converged flames compress the end gases to a high pressure. The laser schlieren and direct flame photographs with observation area of 10 mm in diameter are taken to examine the behaviors of flame front and gas flow in laminar and turbulent combustion. The soot volume fraction in the chamber center during the final stage of combustion at the highest pressure is measured by the in-situ laser extinction technique and simultaneously the corresponding burnt gas temperature by the two-color pyrometry method. The changes of pressure and temperature during soot formation are controlled by varying the initial charging pressure and the volume fraction of inert gas compositions, respectively. It is found that the soot yield increases with dropping the temperature and raising the pressure at a constant equivalence ratio, and the soot yield in turbulent combustion decreases as compared with that in laminar combustion because the burnt gas temperature increases with the drop of heat loss for laminar combustion.

• Journal of Astronomy and Space Sciences
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• v.32 no.4
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• pp.327-333
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• 2015

Previously, all-sky airglow images observed at Shigaraki ($34.9^{\circ}N$, $136.1^{\circ}E$), Japan, during 2004 and 2005 were analyzed in relation to those observed at Mt. Bohyun ($36.2^{\circ}N$, $128.9^{\circ}E$) for a comparison of their gravity wave characteristics (Kim et al. 2010). By applying the same selection criteria of waves and cloud coverages as in the case of Mt. Bohyun all-sky images, we derived apparent wavelengths, periods, phase velocities, and monthly occurrence rates of gravity waves at Shigaraki in this study. The distributions of wavelengths, periods, and speeds derived for Shigaraki were found to be roughly similar to those for Mt. Bohyun. However, the overall occurrence rates of gravity waves at Shigaraki were 36% and 34% for OI 557.7 nm and OH Meinel band airglow layers, respectively, which were significantly higher than those at Mt. Bohyun. The monthly occurrence rates did not show minima near equinox months, unlike those for Mt. Bohyun. Furthermore, the seasonal preferential directions that were clearly apparent for Mt. Bohyun were not seen in the wave propagation trends for Shigaraki. These differences between the two sites imply different origins of the gravity waves near the Korean peninsula and the Japanese islands. The gravity waves over the Japanese islands may originate from sources at various altitudes; therefore, wind filtering may not be effective in causing any seasonal preferential directions in the waves in the airglow layers. Our analysis of the Shigaraki data supports recent theoretical studies, according to which gravity waves can be generated from in situ sources, such as mesosphere wind shear or secondary wave formation, in the mesosphere.

• Ocean and Polar Research
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• v.35 no.1
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• pp.15-26
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• 2013

The toxic, Harmful Algal Blooms (HABs) caused by the Cochlodinium polykrikoides have a serious impact on the coastal waters of Korea. In this study, the acoustic detection system was developed for rapid HABs detection, based on the acoustic backscattering properties of the C. polykrikoides. The developed system was mainly composed of a pulser-receiver board, a signal processor board, a control board, a network board, a power board, ultrasonic sensors (3.5 and 5.0 MHz), an environmental sensor, GPS, and a land-based control unit. To evaluate the performance of the system, a trail was done at a laboratory, and two in situ trials were conducted: (1) when there was no red tide, and (2) when there was red tide. In the laboratory evaluation, the system performed well in accordance with the number of C. polykrikoides in the received level. Second, under the condition when there was no red tide in the field, there was a good correlation between the acoustic data and sampling data. Finally, under the condition when there was red tide in the field, the system successfully worked at various densities in accordance with the number of C. polykrikoides, and the results corresponded with the sampling data and monitoring result of NFRDI (National Fisheries Research & Development Institute). From the laboratory and field evaluations, the developed acoustic detection system for early detecting HABs has demonstrated that it could be a significant system to monitor the occurrence of HABs in coastal regions.

• Asian Journal of Atmospheric Environment
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• v.5 no.1
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• pp.8-20
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• 2011

Atmospheric visibility is one of the indicators used to evaluate the status of air quality. Based on a conceptual definition of visibility as the maximum distance at which the outline of the selected target can be recognized, an image analysis technique is introduced here and an algorithm is developed for visibility monitoring. Although there are various measurement techniques, ranging from bulk and precise instruments to naked eye observation techniques, each has their own limitations. In this study, a series of image analysis techniques were introduced and examined for in-situ application. An imaging system was built up using a digital camera and was installed on the study sites in Incheon and Seoul separately. Visual range was also monitored by using a dual technology visibility sensor in Incheon and transmissometer in Seoul simultaneously. The Sobel mask filter was applied to detect the edge lines of objects by extracting the high frequency from the digital image. The root mean square (RMS) index of variation among the pixels in the image was substantially correlated with the visual ranges in Incheon and Seoul with correlations of $R^2$=0.88 and $R^2$=0.71, respectively. The regression line equations between the visual range and the RMS index in Incheon and Seoul were VR=$2.36e^{0.46{\times}(RMS)}$ and VR=$3.18e^{0.15{\times}(RMS)}$, respectively. It was also confirmed that the fine particles ($PM_{2.5}$) have more impacts to the impairment of visibility than coarse particles.

• Journal of Ginseng Research
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• v.26 no.4
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• pp.213-218
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• 2002

Crystalline calcium oxalate occur throughout near)y all plants species in five major forms; styloids, druses, raphids, prisms and sands. These crystals are known to be distributed in specific tissue such as cortex, xylem, phloem, cambium and epidermis. This research was undertaken to identify the occurrence, type, location and ultrastructure of druse crystals in Panax ginseng. In situ visualization, conventional light microscopy, histochemistry and scanning electron microscopy were applied for these purposes. Druse crystals in ginseng were identified as calcium oxalate by silver nitraterubeanic acid histochemistry. Calcium oxalate crystals are observed in nearly all plant organs such as leaf, petiole, peduncle, stem, rhizome, tap root and lateral root except fine root. Most frequent observation of crystals in the leaf and rhizomes were noticed. Three different types of calcium of oxalate druse crystals were identified by scanning electron microscopy.

• Proceedings of the Korean Vacuum Society Conference
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• 2010.02a
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• pp.380-380
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• 2010

Currently, organic light-emitting diodes (OLEDs) have been proven of their readiness for commercialization in terms of lifetime and efficiency. In accordance with emerging new technologies, enhancement of light efficiency and extension of application fields are required. Particularly inverted structures, in which electron injection occurs at bottom and hole injection on top, show crucial advantages due to their easy integration with Si-based driving circuits for active matrix OLED as well as large open area for brighter illumination. In order to get better performance and process reliability, usually a proper buffer layer for carrier injection is needed. In inverted top emission OLED, the buffer layer should protect underlying organic materials against destructive particles during the electrode deposition, in addition to increasing their efficiency by reducing carrier injection barrier. For hole injection layers, there are several requirements for the buffer layer, such as high transparency, high work function, and reasonable electrical conductivity. As a buffer material, a few kinds of transition metal oxides for inverted OLED applications have been successfully utilized aiming at efficient hole injection properties. Among them, we chose 2 nm of $WO_3$ between NPB [N,N'-bis(1-naphthyl)-N,N'-diphenyl-1,1'-biphenyl-4,4'-diamine] and Au (or Al) films. The interfacial energy-level alignment and chemical reaction as a function of film coverage have been measured by using in-situ ultraviolet and X-ray photoelectron spectroscopy. It turned out that the $WO_3$ interlayer substantially reduces the hole injection barrier irrespective of the kind of electrode metals. It also avoids direct chemical interaction between NPB and metal atoms. This observation clearly validates the use of $WO_3$ interlayer as hole injection for inverted OLED applications.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.468-475
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• 2017

This study is for the consideration of the existence tendency of Kudoa septempunctata in olive flounder. In general, muscle has shown a strong PCR positive reaction in spores containing tissues rather than non-containing tissues. However, blood PCR results showed opposed tendency. In various organs of the tested fish containing spores in muscle tissue, heart had shown positive reaction along with muscle at PCR analysis. Muscle fiber necrosis was observed at the histological observation, and this degeneration was common in both samples. The one sample was the PCR positive muscle containing spore and the other was the PCR positive muscle non-containing spore. Both of muscle tissues indicated a positive reaction at ISH (in-situ hybridization) against K. septempunctata.