• Title/Summary/Keyword: In-Situ experiment

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Statistical Analysis of Soil Moisture using Aircraft Data (항공기 관측 토양수분의 통계 분석)

  • Jeon, Myeon-Ho;Jang, Sun-Woo;Kim, Tae-Woong;Choi, Min-Ha
    • 한국방재학회:학술대회논문집
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    • 2010.02a
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    • pp.103.2-103.2
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    • 2010
  • Soil Moisture Experiment(SMEX)는 2002년부터 2005년까지 해마다 실측치, 항공기 및 원격탐사를 이용하여 토양수분을 관측 및 평가하고 지표와 대기의 수문학적 연구를 위해 기초가 되는 토양수분 자료를 제공한다. 우리나라도 토양수분에 관한 연구가 있지만 원격탐사를 이용한 접근은 적다. 본 연구에서는 SMEX02의 Polarimetric Scanning Radiometer(PSR) 데이터와 In-situ 데이터를 사용가능한 4개 지점에 대하여 비교 분석을 실시하였다. C-band의 토양수분은 10cm 깊이에서 유의함을 보여 주고, 검정을 통하여 토양수분의 분포형은 정규분포 보다 대수정규분포와 Gumbel 분포가 더 유의함을 알 수 있었다.

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Diagnosis of canine distemper by in situ hybridization (In situ hybridization에 의한 개 디스템퍼의 진단)

  • Cho, Hyeon;Park, Nam-yong;Kim, Yong-hwan;Cho, Kyoung-oh;Park, Hyung-seon;Park, Young-seok;Lee, Bong-joo;Chung, Chi-young;Im, Hyung-ho
    • Korean Journal of Veterinary Research
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    • v.39 no.3
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    • pp.583-592
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    • 1999
  • We have developed the in situ hybridization(ISH) technique for rapid diagnosis of canine distemper(CD) which is the major infectious disease in dogs. In our experiment, we rapidly detected distribution of the specific canine distemper viral genome without disrupting morphology of tissues or cells. Two oligonucleotide probes for ISH were synthesized chemically and labelled 5' end with nonisotopic biotin by DNA synthesizer. The whole procedures of ISH was completed within 1~2 hours using the Microcapillary action system. On histological study, typical cytoplasmic or intranuclear inclusion bodies were observed in the trachea, bronchiole, brain, and urinary bladder with the presence of prominent red positive signals on ISH, indicating specific CDV genome from the paraffin-embedded tissues of infected 13 cases. The results showed ISH can be used as a rapid and effective diagnostic method for diagnosis of CD.

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In-Situ Experiment Method on Evaluation of Debris Flow (토석류 발생량 평가를 위한 현장시험 방법)

  • Song, Byungwoong;Yoon, Hyunseok;Kim, Seongmoon
    • Journal of the Korean GEO-environmental Society
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    • v.14 no.7
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    • pp.31-38
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    • 2013
  • After debris flow caused damage during recent years, many scholars and engineers have thrown their effort into analyzing risk from debris flow in Korea. But it is hard to predict damage by debris flow taken place in wide area. Recently, SINMAP program is widely well used to estimate the amount of debris flow and its' range. In order to make frequent use of it, the most important thing is selection of accurate input parameters. In-situ experiments, which are avaliable in the mountain, is to be suggested to get dependable input parameters for SINMAP. Those are permeability, cohesion, density, friction angle and thickness in SINMAP. To get those, test pit, block sampling, in-situ density test, auger boring, permeability test on ground surface, borehole shear test and dynamic cone test and so forth were selected. In addition, the reliability of the results will be increased through comparing with those by laboratory tests. Hence, the experiments are hard to enter the sites without temporary road and, if possible, licensing and many times are needed, too. Small size experiments are indeed necessary to get accurate parameters.

An Experiment for determining Electrical Conductivity in Modelholes using Continuous Measurement System (공내수 전기전도도의 자동측정시스템 구축을 위한 실험)

  • 김영화;박정빈;임헌태
    • The Journal of Engineering Geology
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    • v.13 no.3
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    • pp.281-292
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    • 2003
  • We setup a continuous measurement system for electrical conductivity of fluid in a model borehole and verified the basic environments in electrical conductivity measurement for estimating hydraulic constants. The experiment was made by monitoring the conductivity change within the hole using NaCI solution of different salinities and incoming formation fluid using distilled water. The experiment was made under the state of constant flow rate by maintaining balance between inflow and outflow. Conductivity variation features were observed by controlling salinity contrasts and temperature differences between fluid within the hole and incoming formation fluid. flow rate and the location of inlet and outlet. The results of the experiment show well the role of each affecting factor on the conductivity distribution. and suggest appropriate environments for conductivity measurements. It is considered that the basis of the conductivity measurement for henceforward laboratory model and/or in-situ borehole experiment has been prepared.

Reformability evaluation of blasting-enhanced permeability in in situ leaching mining of low-permeability sandstone-type uranium deposits

  • Wei Wang;Xuanyu Liang;Qinghe Niu;Qizhi Wang;Jinyi Zhuo;Xuebin Su;Genmao Zhou;Lixin Zhao;Wei Yuan;Jiangfang Chang;Yongxiang Zheng;Jienan Pan;Zhenzhi Wang;Zhongmin Ji
    • Nuclear Engineering and Technology
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    • v.55 no.8
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    • pp.2773-2784
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    • 2023
  • It is essential to evaluate the blasting-enhanced permeability (BEP) feasibility of a low-permeability sandstone-type uranium deposit. In this work, the mineral composition, reservoir physical properties and rock mechanical properties of samples from sandstone-type uranium deposits were first measured. Then, the reformability evaluation method was established by the analytic hierarchy process-entropy weight method (AHP-EWM) and the fuzzy mathematics method. Finally, evaluation results were verified by the split Hopkinson Pressure Bar (SHPB) experiment and permeability test. Results show that medium sandstone, argillaceous sandstone and siltstone exhibit excellent reformability, followed by coarse sandstone and fine sandstone, while the reformability of sandy mudstone is poor and is not able to accept BEP reservoir stimulation. The permeability improvement and the distribution of damage fractures before and after the SHPB experiment confirm the correctness of evaluation results. This research provides a reformability evaluation method for the BEP of the low-permeability sandstone-type uranium deposit, which contributes to the selection of the appropriate regional and stratigraphic horizon of the BEP and the enhanced ISL of the low-permeability sandstone-type uranium deposit.

In Situ Solute Migration Experiments in Fractured Rock at KURT: Installation of Experimental System and In Situ Solute Migration Experiments (KURT 암반 단열에서 현장 용질이동 실험: 실험 장치 설치 및 현장 용질 이동 실험)

  • Lee, Jae-Kwang;Baik, Min-Hoon;Lee, Tae-Yeop;Park, Kyung-Woo;Jeong, Jongtae
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.11 no.3
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    • pp.229-243
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    • 2013
  • An in situ solute migration system was designed and installed in KAERI Underground Research Tunnel (KURT) constructed in the site of Korea Atomic Energy Research Institute (KAERI) in order to investigate the migration and retardation of non-sorbing and sorbing tracers through a rock fracture. The system is composed of three main parts including injection, extraction, and data treatment. For the selection of a water-conducting fracture, boreholes were drilled. The fractures in the drilled boreholes were investigated using borehole image analysis using borehole image processing system (BIPS). The results of BIPS analysis showed that borehole YH 3-1 and YH 3-2 were connected each other. Moreover, hydraulic tests were carried out to determine the test section with connectivity for the in situ experiments. The in situ solute migration experiments were accomplished to understand the migration of solutes through fractures in KURT using non-sorbing tracers which were fluorescein sodium, eosin-B, bromide and sorbing tracers which were rubidium, nickel, zirconium, and samarium.

Effects of Supplementation of Synbiotic Co-cultures Manufactured with Anaerobic Microbes on In Vitro Fermentation Characteristics and In Situ Degradability of Fermented TMR (혐기성 미생물로 제조한 synbiotics 혼합배양물의 첨가가 발효 TMR의 발효특성과 소실률에 미치는 영향)

  • Lee, Shin-Ja;Shin, Nyeon-Hak;Hyun, Jong-Hwan;Kang, Tae-Won;An, Jung-Jun;Jung, Ho-Sik;Moon, Yea-Hwang;Lee, Sung-Sill
    • Journal of Life Science
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    • v.19 no.11
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    • pp.1538-1546
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    • 2009
  • This study was conducted to estimate the in vitro fermentation characteristics and in situ degradabilities of total mixed rations fermented by the synbiotic co-cultures composed of various anaerobic microorganisms in the rumen of cow. Seventy two TMR bags (4 treatments $\times$ 6 fermentation days $\times$ 3 replications) were manufactured for in vitro and in situ experiments. The experiment was composed of four treatments including the control, the mould and bacteria synbiotics (T1), the mould and yeast synbiotics (T2) and the bacteria and yeast synbiotics (T3). Each treatment had six fermentation days (1, 3, 5, 7, 14, 21 day) with three replications. Two rumen cannulated Holstein cows (550 ㎏ of mean body wt) were used for in situ trial, and a total of 96 nylon bags were retrieved from the rumen according to eight fermentation times (1, 3, 6, 9, 18, 24, 48 and 72 hr). The mean fermentation temperatures of TMRs by supplementation of anaerobic micoorganism co-cultures ranged from $22.97^{\circ}C$ to $26.07^{\circ}C$, and tended to increase steadily during the entire period. pH values of the F-TMRs ranged from 4.39 to 4.98 and tended to decrease with the extension of the fermentation period, and decreased by supplementation of synbiotics (p<0.05). The ammonia concentrations of F-TMRs were not affected by addition of synbiotic co-cultures during the early fermentation period (within 7 days), but was lowest (p<0.05) in T3 during the late fermentation periods (after 14 days). Lactic acid concentration of F-TMR was lowest in T3 at 1 day of fermentation, but was not different from treatments in the other fermentation days. Microbial growth rates of F-TMR reached a peak at 7 days of fermentation, and afterward tended to decrease. In in situ experiment, the DM disappearance rates were higher in T1 than the control during early fermentation times (within 3 hours), but was vice versa at 48 hours of fermentation (p<0.05). There was no significant difference in effective DM degradability among treatments. NDF and ADF disappearance rates in situ were similar to those of DM. From the above results, the supplementation of synbiotics, particularly the mould and bacteria synbiotics, resulted in improving the pH and concentration of lactic acid of F-TMR as parameters of fermentation compare to the control, and also had higher in situ disappearance rates of DM, NDF and ADF than the control at early fermentation time. However, effective DM degradability was not affected by supplementation of synbiotics.

Analysis of Microbial Community in the TPH-Contaminated Groundwater for Air Sparging using Terminal-Restriction Fragment Length Polymorphism (유류오염대수층 공기분사공정상의 미생물 제한효소다형성법 적용 평가)

  • Lee, Jun-Ho;Lee, Sang-Hoon;Cho, Jae-Chang;Park, Kap-Song
    • Journal of Korean Society on Water Environment
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    • v.22 no.4
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    • pp.590-598
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    • 2006
  • In-situ Air sparging (IAS) is a groundwater remediation technique, in which organic contaminants volatilize into air form the saturated to vadose zone. This study was carried out to evaluate the effect of sludge and soil microbial community structure on air sparging of Total Petroleum Hydrocarbons (TPH) contaminated groundwater soils. In the laboratory, diesel (10,000 mg TPH/kg) contaminated saturated soil. The Air was injected in intermittent (Q=1500 mL/min, 10 minute injection and 10 minute idle) modes. For Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis of eubacterial communities in sludge of wastewater treatment plants and soil of experiment site, the 16S rDNA was amplified by Polymerase Chain Reaction (PCR) from the sludge and the soil. The obtained 16S rDNA fragments were digested with Msp I and separated by electrophoresis gel. We found various sequence types for experiment with sludge soil samples that were closely related to Agrococcus, Flavobacterium, Thermoanaerobacter, Flexibacter and Shewanella, etc, in the clone library. The results of the present study suggests that T-RFLP method may be applied as a useful tool for the monitoring in the TPH contaminated soil the fate of microorganisms in natural microbial community.

Research of the cutting force measuring system using feed drive system built in load cell (이송계에 부착시킨 로드셀을 이용한 절삭력 측정시스템에 관한 연구)

  • 강은구;이상조
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2000.05a
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    • pp.595-598
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    • 2000
  • This paper presents new cutting force measuring system for milling process. Usually, tool dynamometer is the most appropriate measuring tool in an analysis of cutting mechanism. High price and limited space, however, make it difficult to be in-situ system for controllable milling process. Although an alternative using AC current of servomotor has been suggested, it is unsuitable for cutting force control because of low bandwidth and noise. We suggest new cutting force measuring system, using two load cell placed between moving table and nut of ballscrew, and modelled on the system statically and dynamically. And to verify the accuracy of the proposed system, a series of carefully conducted experiments were carried out. Experiment results show that models are in reasonably good agreement with the experiment data.

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Effect of flaking on the digestibility of corn in ruminants

  • Kang, Hamin;Lee, Mingyung;Jeon, Seoyoung;Lee, Sang Moon;Lee, Ju Hwan;Seo, Seongwon
    • Journal of Animal Science and Technology
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    • v.63 no.5
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    • pp.1018-1033
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    • 2021
  • In this study, we aimed to assess the effect of flaking on the nutrient digestibility of corn grain in ruminants. In this regard, in vitro rumen fermentation, in situ rumen degradability, and in vivo metabolic experiments were performed. The automated gas production technique was used for the in vitro fermentation experiments. Six types of corn flakes with various degrees of gelatinization (32%, 41%, 48%, 66%, 86%, and 89%) were ground and incubated in rumen fluid to measure rumen fermentation characteristics and digestion rate. The in situ degradability of ground corn, whole corn, and corn flakes with 62% and 66% gelatinization was measured by incubation in the rumen of two cannulated Holstein cows. In vivo metabolic experiments were performed using 12 crossbred goats (29.8 ± 4.37 kg) using a 3 × 3 Latin square design. The dietary treatments consisted of ground corn and flaked corn with 48% or 62% gelatinization. In vitro experiments showed that as the degree of gelatinization increased, the digestion rate increased linearly, while the discrete lag time decreased linearly (p < 0.05). The effective rumen dry matter degradability, determined by in situ fermentation, was 37%p lower in corn flakes than ground corn, assuming a passage rate of 6%/h (p < 0.01), and there was no difference between the two flakes. In the in vivo experiment, there was no difference in dry matter intake, average daily gain, feed efficiency, and nitrogen utilization among the treatment groups (p > 0.05); however, the crude fat digestibility was lower for corn flakes than for ground corn (p < 0.05). To summarize, the rate of fermentation of corn flakes increased as the degree of gelatinization increased. However, non-ground corn flakes had lower rumen digestibility and did not improve in vivo apparent nutrient digestibility, compared with ground corn. In contrast to the assumption that flaked corn provides more energy to ruminant animals than ground corn, we conclude that the digestibility and energy value of corn flakes are lower than those of ground corn if mastication does not sufficiently reduce the particle size of corn flakes.