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Analysis of Microbial Community in the TPH-Contaminated Groundwater for Air Sparging using Terminal-Restriction Fragment Length Polymorphism  

Lee, Jun-Ho (Department of Environmental Science and Engineering, Hankuk University of Foreign Studies)
Lee, Sang-Hoon (Department of Environmental Science and Engineering, Hankuk University of Foreign Studies)
Cho, Jae-Chang (Department of Environmental Science and Engineering, Hankuk University of Foreign Studies)
Park, Kap-Song (Department of Environmental Science and Engineering, Hankuk University of Foreign Studies)
Publication Information
Journal of Korean Society on Water Environment / v.22, no.4, 2006 , pp. 590-598 More about this Journal
Abstract
In-situ Air sparging (IAS) is a groundwater remediation technique, in which organic contaminants volatilize into air form the saturated to vadose zone. This study was carried out to evaluate the effect of sludge and soil microbial community structure on air sparging of Total Petroleum Hydrocarbons (TPH) contaminated groundwater soils. In the laboratory, diesel (10,000 mg TPH/kg) contaminated saturated soil. The Air was injected in intermittent (Q=1500 mL/min, 10 minute injection and 10 minute idle) modes. For Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis of eubacterial communities in sludge of wastewater treatment plants and soil of experiment site, the 16S rDNA was amplified by Polymerase Chain Reaction (PCR) from the sludge and the soil. The obtained 16S rDNA fragments were digested with Msp I and separated by electrophoresis gel. We found various sequence types for experiment with sludge soil samples that were closely related to Agrococcus, Flavobacterium, Thermoanaerobacter, Flexibacter and Shewanella, etc, in the clone library. The results of the present study suggests that T-RFLP method may be applied as a useful tool for the monitoring in the TPH contaminated soil the fate of microorganisms in natural microbial community.
Keywords
In-situ air sparging (IAS); Microbial community analysis; Polymerase chain reaction (PCR); Terminal-restriction fragment length polymorphism (T-RFLP); Total petroleum hydrocarbons (TPH);
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Times Cited By KSCI : 1  (Citation Analysis)
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