Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
권호 표지

Analysis of Microbial Community in the TPH-Contaminated Groundwater for Air Sparging using Terminal-Restriction Fragment Length Polymorphism

유류오염대수층 공기분사공정상의 미생물 제한효소다형성법 적용 평가

  • Lee, Jun-Ho (Department of Environmental Science and Engineering, Hankuk University of Foreign Studies) ;
  • Lee, Sang-Hoon (Department of Environmental Science and Engineering, Hankuk University of Foreign Studies) ;
  • Cho, Jae-Chang (Department of Environmental Science and Engineering, Hankuk University of Foreign Studies) ;
  • Park, Kap-Song (Department of Environmental Science and Engineering, Hankuk University of Foreign Studies)
  • 이준호 (한국외국어대학교 자연과학대학 환경학과) ;
  • 이상훈 (한국외국어대학교 자연과학대학 환경학과) ;
  • 조재창 (한국외국어대학교 자연과학대학 환경학과) ;
  • 박갑성 (한국외국어대학교 자연과학대학 환경학과)
  • Received : 2005.11.01
  • Accepted : 2006.05.12
  • Published : 2006.07.30
Download PDF
⟨ Previous Next ⟩

Abstract

In-situ Air sparging (IAS) is a groundwater remediation technique, in which organic contaminants volatilize into air form the saturated to vadose zone. This study was carried out to evaluate the effect of sludge and soil microbial community structure on air sparging of Total Petroleum Hydrocarbons (TPH) contaminated groundwater soils. In the laboratory, diesel (10,000 mg TPH/kg) contaminated saturated soil. The Air was injected in intermittent (Q=1500 mL/min, 10 minute injection and 10 minute idle) modes. For Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis of eubacterial communities in sludge of wastewater treatment plants and soil of experiment site, the 16S rDNA was amplified by Polymerase Chain Reaction (PCR) from the sludge and the soil. The obtained 16S rDNA fragments were digested with Msp I and separated by electrophoresis gel. We found various sequence types for experiment with sludge soil samples that were closely related to Agrococcus, Flavobacterium, Thermoanaerobacter, Flexibacter and Shewanella, etc, in the clone library. The results of the present study suggests that T-RFLP method may be applied as a useful tool for the monitoring in the TPH contaminated soil the fate of microorganisms in natural microbial community.

Keywords

Acknowledgement

Supported by : 한국외국어대학교

References

  1. 공성호, 이승희, 조욱상, 곽무영, 고속도로 주변과 주유소의 토양요염에 관한 연구, 한양대학교-환경기술연구소, pp 20-30 (1998)
  2. 김 영, 김진욱, 하철윤, 권수열, 김정관, 이한웅, 하준수, 박후원, 안영호, 이진우, 사염화에틸렌 ( PCE )으로 오염된 국내 4 개 지역 지하수 내 생물학적 PCE 탈염소화 활성 및 미생물 군집의 비교, 한국지하수토양환경학회지 , 10(2), pp. 52-58 (2005)
  3. 송정훈, 장순웅, 이시진, 오염지하수 정화를 위한 공기주입정화법, 산업기술종합연구소 논문집, 23, pp. 129-143 (2002)
  4. 송태을, 상병인, 김만호, 황종식, 미생물 계면활성제를 이용한 오염토양 복원기법과 현장적용성에 관한 연구, 한국토양환경 학회 춘계학술발표회 논문집, pp. 120-123 (1997)
  5. 안경수, 생물을 이용한 오염 토양과 해양의 환경 복원, 인천지역환경기술개발센터, pp. 203-240 (2002)
  6. 안난희, 장덕진, 생물공학의 동향 X I -A: 활성슬러지 내에 서식하는 세균군집의 RFLP 분석, 한국생물공학회지, pp.320-323 (2002)
  7. 장갑열, 원항연, 박정식, 정종천, 공원식, 이강효, 권순익, 오염농경지의 지표미생물 개발, 농업과학기술원 연구보고서, pp. 998-1013 (2004)
  8. 조수형, Lal-scale Biosparging Study to Remediate Dieselcontaminated Soil and Groundwater: the Effect of Air Injection Rate and Pattern, 경기대 대학원 석사학위논문, pp.70-79 (2004)
  9. Autry, A. R. and Ellis, G. M., Bioremediation: an Effective Remedial Alternative for Petroleum Hydrocarbon Contaminated Soil, Environmental progress, 11, pp. 318-323 ( 1992) https://doi.org/10.1002/ep.670110423
  10. Carter, M. R., Soil Sampling and Methods of Methods of Analysis, Lewis, pp. 190-193 (1993)
  11. Cline, S. R., Efficiencies of Soil Washing/Flushing Solutions for the Remediation of Lead Contaminated Soil, Thesis, West Virginia University, pp. 89-98 (1993)
  12. Cookson, J. T., Bioremediation Engineering Design and Application, McGraw-Hill, Inc, pp. 140-149 (1995)
  13. Freeman, H. M. and Harris, E. F., In Situ Soil Flushing, Hazardous Waster Remediation : Innovative Treatment Technologies Edited by Freeman, H. M. and E. F. Harris, Technomic Publishing Company, Inc. pp. 131-138 (1995)
  14. Hinchee, R. E., Air Sparging for Site Remediation, Lewis Publishers, pp. 120-140 (2000)
  15. US EPA, A Technology Assessment of Soil Vapor Extraction and Air Sparging, EPA/600/R-92/173, p. 214 (1992)
  16. Jackson, J. D. and Zenobia, K., Using Microbial Kinetics in the Biorernediation of Contaminated Soil, Remediation of Hazardous Waste Contaminated Soil Edited by Wise, D. L., and Trantolo, D. .I., Marcel Dekker, Inc., pp. 681-689 (1994)
  17. Peterson, J. W., Deboer, M. D. and Lake, K. L., A Simulation of Toluene Cleanup by Air Sparging of Water Saturated Sands, Journal of Hazardous Materials, 72, pp. 167-178 (2000) https://doi.org/10.1016/S0304-3894(99)00139-9
  18. Peterson, .J. W., Lepczyk, P. A. and Lake, K. L., Effect of Sediment Size on Area of Influence During Groundwater Remediation by Air Sparging : A Laboratory Approach, Environmental Geology, 38, pp. 1-6 (1999) https://doi.org/10.1007/s002540050394
  19. Zhou, G. W., Ong, W. Z. and Chen, T., Development of a Fungus-specific PCR Assay for Detecting Low-level Fungi in an Indoor Environment, Molecular and Cellular Probes, 14(6), pp. 339-348 (2000) https://doi.org/10.1006/mcpr.2000.0324