• 대한토목학회논문집
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• 제26권3D호
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• pp.499-509
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• 2006

프리캐스트 콘크리트 바닥판은 일반적인 현장타설 바닥판과 비교할 때 많은 장점을 가지고 있으며, 최근 국내에서도 도로교의 노후 바닥판 교체 공사 및 신설 도로교에 성공적으로 적용되고 있다. 하지만, 철도교에 프리캐스트 바닥판을 적용하기 위해서는 활하중, 장대레일 종하중, 시제동하중, 온도변화, 건조수축 등 도로교와 철도교의 하중 특성의 차이를 적절히 반영한 설계가 요구되며, 특히 횡방향 이음부의 연속성을 확보하여 일체화시키기 위해 바닥판 내부에 배치한 종방향 텐던의 적정한 프리스트레스 수준을 평가하는 연구가 요망된다. 따라서 본 연구에서는 철도교에 빈번히 적용되는 PSC 합성거더 철도교에 대하여 정밀한 3차원 유한요소해석을 통해 주요 설계하중이 유발하는 바닥판의 종방향 응력을 산정하였다. 그 결과 종방향 인장응력을 유발하는 지배적인 요인은 활하중보다는 바닥판의 온도 저하나 시동하중으로 나타났으며, 특히 온도의 영향은 설계기준상의 제안식 또는 이론식과도 비교해 가며 중점적으로 분석하였다. 종방향 인장응력을 상쇄하여 올바른 구조거동을 유도하기 위한 프리스트레스의 범위를 고찰한 결과, 대상 철도교에 대해 2.4 MPa 이상의 프리스트레스를 도입하는 것이 적정한 것으로 나타났으며 이는 도로교의 경우와 유사한 수준으로 평가되었다.

• 지질공학
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• 제27권3호
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• pp.233-244
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• 2017

주입액의 점성도와 응력상태에 따른 균열전파 특성을 분석하기 위해 실험실 규모의 수압파쇄시험을 실시하였다. 시험에 사용된 시료는 시멘트 몰탈을 사용하여 제작되었으며, 각 변의 길이가 20 cm인 정육면체 형태이다. 제작된 시료는 최대강도를 갖기 위해 수중에서 약 1달간 양생과정을 거쳤다. 독립적인 가압시스템을 가지고 있는 진삼축압축장치로 시료에 압력을 가하여, 실제의 지반에서 작용하는 원위치응력 상태를 재현하였다. 시추 환경 재현을 위해 시료에 소형 시추공을 천공한 후, 일정한 주입속도로 수압파쇄시험을 실시하였다. 수압파쇄시험 과정에서 시추공에 주입된 유체의 압력을 실시간으로 측정하였으며, 동시에 미소파괴음(AE) 신호를 측정하였다. 수압파쇄시험의 모든 과정이 끝난 후 생성된 균열의 형태를 육안으로 관찰하였다. 일차파쇄압력은 주입액의 점성도 증가에 따라 지수형태를 보이며 증가하였다. 수압파쇄시험으로 인해 생성된 균열의 형태는 최대주응력과 최소주응력의 차이인 편차응력의 크기에 따라 서로 다른 양상을 보였다. 낮은 편차응력의 조건에서는 단일의 균열이 아닌 다중 균열이 생성되거나, 균열 성장과정에서 방향이 휘어지는 경향을 보였고, 이에 반해 높은 편차응력의 조건에서 생성된 균열은 단일 면상의 균열이 발생하였다. AE 분석에서도 편차응력이 클수록 미세균열이 단일 면상으로 집중되어 발생되는 경향을 보였다. 이러한 연구결과는 수압파쇄 방법을 이용한 암반파쇄에서 편차응력이 클 때보다 작을 때 더 복잡한 균열이 발생된다는 것을 보여준다. 따라서 셰일가스를 개발할 때 생산량을 높이기 위해서는 복잡한 균열을 발생시킬 수 있는 편차응력이 작은 조건에서 수압파쇄가 적용되는 것이 효과적일 것으로 판단된다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권9호
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• pp.5007-5010
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• 2013

Heat shock proteins are molecular chaperones that may be constitutively present in cells protecting them from various stresses, such as extreme temperature, anoxia or chemical agents. Cervical cancer is the second most prevalent malignancy of women. In this study, we analyzed the expression of Hsp27 by immunohistochemistry in cervical intraepithelial lesions of Brazilian women, along with samples from non neoplasic lesions (NN). Cervical intraepithelial neoplasia I (CIN I), II (CIN II) and III (CIN III)/in situ carcinoma and squamous cell carcinoma (SCC) were included. Immunostaining was observed in 30 (100%) samples of NN, 46 (92%) in CIN I, 50 (100%) in CIN II, 52 (98.11%) in CIN III/CIS, and 46 (98.11%) in SCC. In group NN Hsp27 immunostaining was heterogeneous, more intense in basal and parabasal layers of the epithelium and less or absent in the intermediate and superficial layer. The majority of the samples of CIS and SCC presented strong staining in all epithelial layers. Metaplasic cells, when present, were strongly stained. In this study, Hsp27 protein was found to be commonly expressed in cervical epithelial cells.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2005년도 국제학술심포지움 The 44th Annual Meeting of Korean Society for Life Science
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• pp.23-36
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• 2005

Bax, a mammalian pro-apoptotic member of the Bcl-2 family, induces cell death when expressed In yeast. To investigate whether .Bax expression can induce cell death in plant, we produced transgenic Arabidopsis plants that contained murine Bax cDNA under control of a glucocorticoid-inducible promoter. Transgenic plants treated with dexamethasone, a strong synthetic glucocorticoid, induced Bax accumulation and cell death, suggesting that some elements of cell death mechanism by Bax may be conserved among various orgarusms. Therefore, we developed novel yeast genetic system, and cloned several Plant Bax Inhibitors (PBIs). Here, we report the function of two PBIs In detail. PBIl is ascorbate peroxidase (sAPX). Fluorescence method of dihydrorhodamine123 oxidation revealed that expression of Bax in yeast cells generated reactive oxygen species (ROS), and which was greatly reduced by co-expression with sAPX. These results suggest that sAPX inhibits the generation of ROS by Bax, which in turn suppresses Bax-induced cell death in yeast. PBI2 encodes nucleoside diphosphate kinase (NDPK). ROS stress strongly induces the expression of the NDPK2 gene in Arabidopsis thaliana (AtNDPK2). Transgenic plants overexpressing AtNDPK2 have lower lovels of ROS than wildtype plants. Mutants lacking AtNDPK2 had higher levels of ROS than wildtype. H$_{2O2}$ treatment induced the phosphorylation of two endogenous proteins whose molecular weights suggested they are AtMPK3 and AtMPK6. In the absence of H2O2 treatment, phosphorylation of these proteins was slightly elevated in plants overexpressing AtNDPK2 but markedly decreased In the AtNDPK2 deletion mutant. Yeast two-hybrid and in vitro protein pull-down assays revealed that AtNDPK2 specifically interacts with AtMPK3 and AtMPK6. Furthermore, AtNDPK2 also enhances the MBP phosphorylation activity of AtMPK3 i'n vitro. Finally, constitutive overexpression of AtNDPK2 in Arabidopsis plants conferred an enhanced tolerance to multiple environmental stresses that elicit ROS accumulation In situ. Thus, AtNDPK2 appears to play a novel regulatory role in H2O2-mediated MAPK signaling in plants.

• Geomechanics and Engineering
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• 제8권2호
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• pp.187-220
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• 2015

Soil disturbance induced by installation of mandrel driven vertical drains decreases the in situ horizontal hydraulic conductivity of the soil in the vicinity of the drains, decelerating the consolidation rate. According to available literature, several different profiles for the hydraulic conductivity variation with the radial distance from the vertical drain, influencing the excess pore water pressure dissipation rate, have been identified. In addition, it is well known that the visco-plastic properties of the soil also influence the excess pore water pressure dissipation rate and consequently the settlement rate. In this study, a numerical solution adopting an elastic visco-plastic model with nonlinear creep function incorporated in the consolidation equations has been developed to investigate the effects of disturbed zone properties on the time dependent behaviour of soft soil deposits improved with vertical drains and preloading. The employed elastic visco-plastic model is based on the framework of the modified Cam-Clay model capturing soil creep during excess pore water pressure dissipation. Besides, nonlinear variations of creep coefficient with stress and time and permeability variations during the consolidation process are considered. The predicted results have been compared with V$\ddot{a}$sby test fill measurements. According to the results, different variations of the hydraulic conductivity profile in the disturbed zone result in varying excess pore water pressure dissipation rate and consequently varying the effective vertical stresses in the soil profile. Thus, the creep coefficient and the creep strain limit are notably influenced resulting in significant changes in the predicted settlement rate.

• 국제학술발표논문집
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• The 1th International Conference on Construction Engineering and Project Management
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• pp.655-661
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• 2005

For a hydro power plant project, the headrace tunnel having a finished diameter of 3.3m was constructed in volcanic rocks with well-developed vertical joint and high groundwater table. The intake facility was located 20.3 km upstream of the powerhouse and headrace tunnel of 20 km in length and penstock of 440 m in height connected the intake and the powerhouse. The typical caldera lake, Lake Toba set the geology at the site; the caving of the ground caused tension cracks in the vertical direction to be developed and initial stresses at the ground to be released. High groundwater table(the maximum head of 20 bar) in the area of well-connected vertical joints delayed the progress of tunnel excavation severely due to the excessive inflow of groundwater. The excavation of tunnel was made using open-shield type TBM and mucking cars on the rail. High volume of water inflow raised the water level inside tunnel to 70 cm, 17% of tunnel diameter (3.9 m) and hindered the mucking of spoil under water. To improve the productivity, several adjustments such as modification of TBM and mucking cars and increase in the number of submersible pumps were made for the excavation of severe water inflow zone. Since the ground condition encountered during excavation turned out to be much worse, it was decided to adopt PC segment lining instead of RC lining. Besides, depending on the conditions of the water inflow, rock mass condition and internal water pressure, one of the invert PC segment lining with in-situ RC lining, RC lining and steel lining was applied to meet the site specific condition. With the adoption of PC segment lining, modification of TBM and other improvement, the excavation of the tunnel under severe groundwater condition was successfully completed.

• Journal of Plant Biotechnology
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• 제6권2호
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• pp.77-85
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• 2004

Development of efficient plant regeneration and genetic transformation protocols (using the Particle Inflow micro-projectile Gun and the shoot-tips as target tissue) of Sorghum bicolor (L.) Moench in terms of expression of the reporter gene, $\beta$-glucuronidase(uidA) is reported here. Two Indian cultivars of sorghum were used in the study, viz. M-35-1 and CSV-15. Plant regeneration was achieved from one-week-old seedling shoot-tip explants via multiple-shoot-clumps and also somatic embryos. The multiple-shoot-clumps were produced on MS medium containing BA (0.5, 1.0 or 2.0 mg/$L^{-1}$), with biweekly subculture. Somatic embryos were directly produced on the enlarged dome shaped expansive structures that developed from shoot-tip explants (without any callus formation) when cultured on MS medium supplemented both with BA (0.5, 1.0 or 2.0 mg/$L^{-1}$) and 2,4-D (0.5 mg/$L^{-1}$). Whereas each multiple-shoot-clump was capable of regenerating more than 80 shoots via an intensive differentiation of both axillary and adventitious shoot buds, the somatic embryos were capable of 90% germination, plant conversion and regeneration. The regenerated shoots could be efficiently rooted on MS medium containing 1.0mg/$L^{-1}$ IBA and successfully transplanted to the glasshouse and grown to maturity with a survival rate of 92%. The plant regeneration efficiency of both the genotypes were similar. After the micro-projectile bombardment, expression of uidA gene was determined by scoring blue transformed cell sectors in the bombarded tissue by an in situ enzyme assay. The optimal conditions comprising a helium pressure of 2200 K Pa, the target distance of 11 cm with helium inlet fully opened and the use of osmoticum have been defined to aid our future strategies of genetic engineering in sorghum with genes for tolerance to biotic and abiotic stresses.

• 한국초전도ㆍ저온공학회논문지
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• 제21권1호
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• pp.40-44
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• 2019

This study investigates mixtures of water and cryoprotectant agents (CPAs) to store high-grade cold energy. Although water is an ideal material for a cold thermal storage (CTS) due to its high specific heat, undesirable volume expansion may cause structural stresses during freezing. The volume expansion can be alleviated by adding the CPAs to water. However, the CPA aqueous solutions not only have different thermal properties but also transit to amorphous state different from pure water. Therefore, these characteristics should be considered when using them as material of the CTS. In experiments, glycerol and dimethyl sulfoxide (DMSO) are selected as the candidate CPA. The volume expansion of the solution is measured by an in-situ strain gauge in low temperature region. The specific heat capacity of the solution is also measured by differential scanning calorimetry (DSC). Both the amount of volume expansion and the specific heat capacity of the CPA aqueous solution decrease in the case of higher concentration of CPA. These characteristics should be contemplated to select optimal aqueous solution for CTS for liquid air energy storage system (LAES). The CPA solutions have advantages of having wide temperature range to utilize the latent heat of water and higher sensible heat of the CPA. The CPA solutions which can satisfy the allowable stress of the structure are determined. Consequently, among the CPA solutions investigated, DMSO 20% w/w solution is the most suitable for the CTS.

• 한국터널지하공간학회 논문집
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• 제4권3호
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• pp.235-245
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• 2002

본 연구는 터널 굴착시 막장 전방의 지반변화를 사전에 예측할 수 있는 방법을 제시하고자 하였다. 이를 위하여 막장 전방에 파쇄대가 방향성을 가지고 존재 할 때 터널의 3차원 해석을 통하여 터널의 내공변위의 변화 경향을 살펴보았으며, 이를 통하여 막장전방의 지반 변화를 파악하고자 하였다. 이러한 터널의 내공변위의 변화는 경향선과 영향선을 이용하여 표현할 수 있으며 이를 이용하여 막장 전방에 존재하는 파쇄대를 예측하고자 하였다. 수치해석 결과에 의하면 막장전방에 파쇄대가 존재 할 경우 막장이 파쇄대에 접근할 수록 급격한 경향선의 변화가 나타난다. 또한 파쇄대가 방향성을 가지고 있는 경우에는 경향선의 급격한 변화 외에 측벽부의 변위 경향이 비대칭을 이루므로 인하여 평사투영도 상에 나타난 변위가 비대칭을 형성하는 것으로 파악되었다. 이러한 수치해석결과에 의한 내공변위 해석결과를 현장 계측 자료와 비교하였으며, 현장에서 계측한 오차를 줄일 수 있도록 계측데이터가 정규분포 한다고 가정하여 현장데이터를 분석한 결과 막장전방의 파쇄대의 존재 유무를 파악할 수 있는 것으로 나타났다.

• 마이크로전자및패키징학회지
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• 제6권2호
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• pp.23-30
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• 1999

이성질체에 따른 폴리이미드 박막의 잔류응력 영향과 모폴로지와의 상관관계가 조사되었다. 이를 위해, Poly(phenylene biphenyltetracarboximide) (BPDA-PDA)와 poly (oxydiphenylene biphenyltetracar-boximide) (BPDA-ODA)를 여러 다른 diamine인 1, 3-phenylene diamine (1, 3-PDA), 1.4-phenylene diamine (1,4-PDA)과 3.4'-oxydiphenylene diamine (3,4'-ODA) , 4,4'-oxydiphenylene diamine (4.4'-ODA)으로부터 제조하였다. 이들 박막에 대하여, Thin Film Stress Analyzer (TFSA)를 이용하여 공정온도 (25~$400^{\circ}C$)하에서 전구체의 열적 이미드화에 따라 실시간으로 폴리이미드 박막의 잔류응력 거동을 측정하였다. 폴리이미드 박막의 잔류응력은 면 방향 배향성과 사슬 질서도가 우수한 BPDA-1,4-PDA가 7MPa로 가장 낮게 나타났으며 BPDA-1,3-PDA, BPDA-3,4'-ODA, BPDA-4,4'-ODA의 경우 40~50Mpa 범위에 있었다. 이성질체에 따른 폴리이미드 박막의 잔류응력은 모폴로지 (사슬 강직도, 질서도, 배향성) 변화 및 유리전이 거동과 관련된 사슬 운동성을 이용하여 분석되었다.