• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.4
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• pp.403-410
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• 2014

The purpose of this study was to examine the anti-depressive effects of OnDam-Tang with addition of Linderae Radix (ODT-L) on the animal model of depression induced by chronic immobilization stress. Depression model was made by chronic immobilization stress for 2 hours for 21 days. And we performed forced swimming test, analysis of the neurotransmitter and immunohistochemical staining, measured expression levels of serotonin in the brain. ODT-L has decreased immobilization time in forced swimming test. ODT-L has increased amount of melatonin in the brain. ODT-L has increased expression levels of serotonin in the brain. ODT-L prevented damage in the hippocampal region. ODT-L has reduced the expression level of CRF receptors in in hippocampus region. These results suggest that ODT-L may have anti-depressive effects on depression.

• BMB Reports
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• v.31 no.2
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• pp.117-122
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• 1998

Changes of lipocortin 1 (LC1) in the brain induced by immobilization stress were investigated in rats. Rats were immobilized for 0,1,2,3,4, and 5 h, and the brain slices were immunostained with anti-human LC1 antibodl (anti-LC1). Immunoreactivity of LCI (iLC1) was most prominent in neuronal cell bodies and processes of hippocampal CA regions and dentate gyrus. At rest without stress, most of the LC1 in the neuron located in the cytoplasm with the nuclei exhibiting relatively scarce immunoreactivity. Immobilization stress changed this intracellular distribution of LC1 by increasing nuclear LC1. The change was apparent in 1 h and reached the peak by 3 h. However, by 5 h of immobilization, the distribution pattern returned to that of the resting state. This transient nuclear translocation of LC1 was most prominent in $CA_1$ pyramidal neurons, and was not observed in areas other than the hippocampus. Adrenalectomy abolished this transient translocation of LC1. The roles of hippocampal LC1 as a mediator of glucocorticoid feedback signal and/or as an intracellar stress signaling protein could be suggested.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.1
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• pp.172-178
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• 2004

To investigated the effects of Kwibi-tang water extract (KBT) on the non-specific immune response in C57BL/6 mice stressed by immobilization, we evaluated the changes in the contents of serum histamine and corticosterone and the phagocytic activity of macrophages. The level of serum histamine and corticosterone was determined with spectrofluorometer. The cell viability was determined by a MTT assay method. The subpolpulation of lymphocytes was determined by a flow cytometry. The phagocytic activity was determined with luminometer. KBT decreased the serum level of histamine and corticosterone increased by immobilization stress. Also, KBT enhanced the phagocytic activity and decreased the level of nitric oxde in murine peritoneal macrophages decreased by immobilization stress. These results indicate that KBT may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of the non-specific immune response.

• Journal of Haehwa Medicine
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• v.10 no.1
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• pp.489-494
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• 2001

To determine the preventive effect of Samulanshintang (SA) on stress, we investigated the physiological change of rats which were applied immobilization stress. For immobilization stress, rats were placed in restrainer for 12 hours a day for 3 days. During application of stress, body weight of rats was measured. After sacrifice, 4 organs were taken for measurement of organ weight. Brain was homogenated and its catecholamine and serotonin contents were measured with HPLC. In our study, stress mainly induced increase of concentration of neurotransmitters in brain without other significant physical change of rats. SA inhibited stress induced changes of neurotransmitter content in brain.

• The Journal of Korean Medicine
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• v.20 no.1 s.37
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• pp.172-184
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• 1999

This study was aimed to evaluate the anti-stress effect of Kungchihwadamjeun on the body weight. organs weight, S- GOT. S-GPT. lipid peroxidase in organs and contents of cathecholamine in brain of rats stressed by immobilization. The main results obtained were summarized as follow 1. The body weights increased in the group treated with Kungchihwadamjeun of rats stressed by immobilization compared with in the group treated with non Kungchihwadamjeun. 2. The weight of the spleen. kidney. pancreas, thymus and heart increased in the group treated with Kungchihwadamjeun of rats stressed by immobilization compared with in the group treated with non Kungchihwadamjeun. 3. The contents of serum GOT GPT decreased in the group treated with Kungchihwadamjeun of rats stressed by immobilization compared with in the group treated with non Kungchihwadamjeun 4. The lipid peroxidase in liver decreased in the group treated with Kungchihwadamjeun of rats stressed by immobilization compared with in the group treated with non Kungchihwadamjeun. 5. The contents of norepinephrine and serotonine in brain decreased in the group treated with Kungchihwadamjeun of rats stressed by immobilization compared with in the group treated with non Kungchihwadamjeun, but epinephrine and dopamine in brain increased. According to the above results, this experiments concluded that Kungchihwadamjeun had significant effects in reducing stress.

• Journal of Genetic Medicine
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• v.4 no.2
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• pp.179-185
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• 2007

Purpose : Catecholamines are the neuro-transmitters in the sympathetic nervous system (SNS) and are activated by stress stimulus. Tyrosine hydroxylase (TH) and Dopamine-${\beta}$-Hydroxylase (DBH) are very important enzymes in the catecholamine synthesis. Corticotropin releasing hormone (CRH) is released in the process of reacting to stresses. The aim of this study is to find out what effects immobilization stresses have on the expression of TH, BDH and CRH mRNA in a rat's brains. Methods : We compare expression levels in rat's brains of TH, DBH and CRH mRNA induced by immobilization stresses between the test group and controled group. The expression levels of TH, DBH and CRH mRNA are measured by RT-PCR and the Western Blotting Analysis (WBA). Results : In brains and adrenal glands of the immobilization stress group, the expression levels of TH and DBH mRNAs are significantly two to three times higher (P<0.01), and CRH mRNAs are approximately one and a half times higher (P<0.05) than those of controlled group. Conclusion : This study suggest that the expression levels of TH, DBH and CRH mRNAs are activated by stress stimulus in a rat's brains and adrenal glands.

• The Journal of Internal Korean Medicine
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• v.34 no.3
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• pp.256-266
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• 2013

Objectives : The purpose of this study was to investigate the protective effects of Magnolia Officinalis extracts on the animal model of depression induced by immobilization stress. Methods : The subjects were divided into 4 groups : normal, saline solution-administered during immobilization stress, 200 mg/kg of magnolia extracts-administered (magnolia extract 200), and 400 mg/kg of magnolia extracts- administered (magnolia extract 400). During 2 days of immobilization stress treatment, they underwent forced swimming test (FST) and tail suspension test (TST). The number of serotonin (5-HT) immunostained nuclei in the dorsal raphe nucleus regions was measured by immunohistochemistry. Superoxide dismutase (SOD) and glutathione peroxidase (GPX) in blood were measured. Results : In FST, magnolia-administered groups showed significantly decreased immobilization. In TST, the magnolia extract 400 group showed decreased immobilization. The stress group showed significantly decreased number of 5-HT immunostained nuclei in the dorsal raphe nucleus regions, while magnolia extract 400 group showed increased number of 5-HT immunostained nuclei. Stress group showed decrease in serum level of SOD and GPX, while the magnolia extract 200 group showed increase in serum level of SOD and GPX. Conclusions : These results suggest potent effectiveness of magnolia extracts in the treatment of depression.

• Nutrition Research and Practice
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• v.4 no.4
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• pp.270-275
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• 2010

Catecholamines are among the first molecules that displayed a kind of response to prolonged or repeated stress. It is well established that long-term stress leads to the induction of catecholamine biosynthetic enzymes such as tyrosine hydroxylase (TH) and dopamine ${\beta}$-hydroxylase (DBH) in adrenal medulla. The aim of the present study was to evaluate the effects of ginseng on TH and DBH mRNA expression. Repeated (2 h daily, 14 days) immobilization stress resulted in a significant increase of TH and DBH mRNA levels in rat adrenal medulla. However, ginseng treatment reversed the stress-induced increase of TH and DBH mRNA expression in the immobilization-stressed rats. Nicotine as a ligand of the nicotinic acetylcholine receptor (nAChR) in adrenal medulla stimulates catecholamine secretion and activates TH and DBH gene expression. Nicotine treatment increased mRNA levels of TH and DBH by 3.3- and 3.1-fold in PC12 cells. The ginseng total saponin exhibited a significant reversal in the nicotine-induced increase of TH and DBH mRNA expression, decreasing the mRNA levels of TH and DBH by 57.2% and 48.9%, respectively in PC12 cells. In conclusion, immobilization stress induced catecholamine biosynthetic enzymes gene expression, while ginseng appeared to restore homeostasis via suppression of TH and DBH gene expression. In part, the regulatory activity in the TH and DBH gene expression of ginseng may account for the anti-stress action produced by ginseng.

• Herbal Formula Science
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• v.10 no.2
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• pp.97-112
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• 2002

In order to investigate the effect of Sagoonja-Tang, Samool-Tang and Yookmijihwang-Tang against the cold, heat and immobilization stress, each formula was injected intragastically to mice exposed to forced cold water swimming($4^{\circ}C$), forced hot water swimming($42^{\circ}C$) and immobilization stress before measuring the change of plasma corticosterone level of mice. The results were as follows: 1. One hour after each Sagoonja-Tang, Samool-Tang, Yookmijihwang-Tang with the dose of 1g／kg, 3g／kg each were administered to mice, we put cold stress($4^{\circ}C$) on mice for 3min. Then those prescriptions didn't show any significant effect on plasma corticosterone level. however, Yookmijihwang-Tang(1g/kg) showed a significant increase(p〈0.05), and Samool-Tang(3g/kg) showed a small increase. 2. One hour after Samool-Tang, Sagoonja-Tang and Yookmijihwang-Tang with the dose of 1g／kg, 3g／kg each were administerd, mice exposed to heat stress($42^{\circ}C$) for 3 min. Samool-Tang(1g/kg) and Yookmijihwang-Tang(lg/kg) showed significant decrease of corticosterone level(p〈0.01, p〈0.05) but Sagoonja-Tang didn't change it. In the case of the dose of 3g/kg, only Samool-Tang decreased corticosterone level(P〈0.01), but Sagoonja-Tang, Yookmijihwang-Tang didn't make significant effect on plasma corticosterone level of the mice exposed to heat stress($42^{\circ}C$ ). 3. Those mice which exposed to immobilization stress one hour after Sagoonja-Tang, Samool-Tang, Yookmijihwang-Tang with the dose of 1g/kg and 3g/kg didn't show effective decrease of corticosterone level. However, Sagoonja-Tang(3g/kg), Samool-Tang(lg/kg, 3g/kg) showed the effect of mild increase of corticosterone level rather than decrease. These data revealed that the effect-variation of prescriptions depended on the kinds of stress and the dose of prescriptions had a different effect on therapy.

• Journal of Nutrition and Health
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• v.27 no.2
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• pp.153-161
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• 1994

We fed high trypotophan diet(3.5% tryptophan/diet(w/w) to mice for 7 days and treated then with 3 hour immobilization(IMMB) stress to investigate tryptophan metabolism and immunomodulation. The levels of serum tryptophan, brain tryptophan, serotonin(5HT) and 5-hydroxyindoleacetic acid(5HIAA) in the tryptophan diet fed animals were higher than those of the normal diet fed animals. Feeding tryptophan supplemented diet to stressed animal significantly decreased the levels of serum and brain tryptophan and 5HT levels. However, the amount of 5HIAA which is the metabolite of serotonin was increased in brain. Plasma corticosterone level was increased by the stress in both groups but the degree of this increase was smaller in high tryptophan fed animals. The relative numbers of CD8+ T cells, CD4+ T cells and B cells in spleen were decreased in high tryptophan diet fed and stressed animals compared to control diet fed and no stressed animals. CD8+ T cells decreased more than CD4+ T cells. The decrease of CD8+ T cells in high tryptophan fed and stressed animals was similar to that in high tryptophan fed animals or normal diet fed and stressed animals. Stress and tryptophan supplement acted synergistically to decrease the number of B cells. This study suggests that stress and tryptophan supplement could modify the number of lymphocyte cells, and indicates that the interaction of stress and tryptophan supplement on immune fuction depends on the types of immune cells.