• Journal of Biosystems Engineering
• /
• v.37 no.2
• /
• pp.113-121
• /
• 2012

Purpose: Particle separation in solution is one of important process in a unit operation as well as in an extract preparation for biosensors. Contrary to centrifuge-type of mesh-type filter, using an ultrasonic standing wave make the filtering process continuous and free from maintenance. It is needed to investigate the characteristics of particle movement in the ultrasonic standing wave field. Methods: Through the computer simulation the effects of major design and driving parameters on the alignment characteristics of particles were investigated, and a cylindrical chamber with up-stream flow type was devised using two circular-shape PZTs on both sides of the chamber, one for transmitting ultrasonic wave and the other for just reflecting it. Then, the system performance was experimentally investigated as well. Results: The speed of a particle to reach pressure-node plane increased as the acoustic pressure and size of particle increased. The maximum allowable up-stream flow rate could be calculated as well. As expected, exact numbers of pressure-node planes were well formed at specific locations according to the wavelength of ultrasonic wave. As the driving frequency of PZT got close to its resonance frequency, the bands of particles were observed clearer, which meant the particles were trapped into narrower space. Higher excitation voltages to the PZT produced a greater acoustic force with which to trap particles in the pressure-node planes, so that the particles gathered could move upwards without disturbing their alignments even at a higher inlet flow rate. Conclusions: This research showed the feasibility of particle separation in solution in the continuous way by an ultrasonic standing wave. Further study is needed to develop a device to collect or harvest those separated particles.

• Journal of Animal Science and Technology
• /
• v.60 no.6
• /
• pp.10.1-10.6
• /
• 2018

Background: Stress causes inflammation that impairs intestinal barrier function. Dietary spray-dried plasma (SDP) has recognized anti-inflammatory effects and improvement of gut barrier function. Therefore, the purpose of this study was to investigate the effects of dietary SDP on intestinal morphology of mated female mice under stress condition. Results: Villus height, width, and area of small intestines were low on gestation day (GD) 3 or 4 under stress conditions, and higher later (Time, P < 0.05). Crypt depth of colon was low on GD 4 and higher later (Time, P < 0.05). Meanwhile, the SDP treatments improved (P < 0.05) intestinal morphology, indicated by increased villus height, villus width, villus area, and ratio between villus height and crypt depth of small intestines and crypt depth of colon, and by decreased crypt depth of small intestines, compared with the control diet. The SDP treatments also increased (P < 0.05) the number of goblet cells in intestines compared with the control diet. There were no differences between different levels of SDP. Conclusion: Dietary SDP improves intestinal morphology of mated female mice under stress condition.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.60 no.4
• /
• pp.470-474
• /
• 2015

This study was carried out to compare botanical and amylogram characteristics including table quality in waxy corn kernel. The used materials in this experiment were producted and evulated at Corn Breeding Laboratory, Coll. of Agri. & Life Sci., in CNU. In botanical characteristics CNU H09-26 among used hybrids was highest in stem height as 228.5 cm, but that of CNU H09-30 was lowest. Ear height was highest in CNU H09-23 as 78.2 cm, but that of CNU H09-30 was lowest. Ear length among hybrids were also variable as 21.2 cm to 10.8 cm. in amylogram analysis CNU H09-23 hybrid was lowest in pasting temperature, while break down of this hybrid was highest These results appeared highly in table quality. Accordingly we thought that this hybrid will be adapted as a leading variety for edible waxy corn.

• Journal of Korean Society for Geospatial Information Science
• /
• v.20 no.3
• /
• pp.3-10
• /
• 2012

The investigation of valley networks and their volume provide important information about past water activities on Mars. As an alternative of conventional image processing methods, terrain filtering algorithm using pointcloud data is suggested in this study. First, the topography of pointcloud is inverted so that the valleys become positive features and the algorithm is then applied to distinguish the valleys from the surface. Ground DEM and object DEM are generated from both the valleys and the surface pointcloud then the volume of valleys is estimated by multiplying the height difference between the surface with valleys and the area of valleys based on grid cellsize. In the test of valleys adjacent to Tuscaloosa crater, the total volume of valleys was estimated to be $1.41{\times}10^{11}m^3$ with the difference of 12% and 16% compared with the infill volume of Tuscaloosa crater and BTH result respectively.

• Journal of the Korean GEO-environmental Society
• /
• v.2 no.2
• /
• pp.13-22
• /
• 2001

In this study, centrifuge model tests were fulfilled to investigate the characteristics of bearing capacity of paper ash as a filling material. The model tests were done varying the footing width and gravity level. The settlement and vertical soil pressure by loading were measured. The results from the tests were compared with the one from FLAC program using finite difference method and bearing capacity theory. After all, it was shown that the characteristics of load-settlement represented the local shear failure, which the settlement ratio s/B showed inflection point around 25~30%. As g-level and footing width were increasing, the load strength was increasing. The ultimate bearing capacity from the tests was very closed the results from Terzaghi's theory. As the distance from footing center was increasing, the vertical soil pressure was decreasing. If E/B is higher than 7, the stress by loading was almost increasing. The vertical displacement from loading was the largest one around under the footing and was almost occurred when the depth>4cm and E/B is higher than 5.0.

• Genomics & Informatics
• /
• v.13 no.4
• /
• pp.137-145
• /
• 2015

Selective sweep can cause genetic differentiation across populations, which allows for the identification of possible causative regions/genes underlying important traits. The pig has experienced a long history of allele frequency changes through artificial selection in the domestication process. We obtained an average of 329,482,871 sequence reads for 24 pigs from three pig breeds: Yorkshire (n = 5), Landrace (n = 13), and Duroc (n = 6). An average read depth of 11.7 was obtained using whole-genome resequencing on an Illumina HiSeq2000 platform. In this study, cross-population extended haplotype homozygosity and cross-population composite likelihood ratio tests were implemented to detect genes experiencing positive selection for the genome-wide resequencing data generated from three commercial pig breeds. In our results, 26, 7, and 14 genes from Yorkshire, Landrace, and Duroc, respectively were detected by two kinds of statistical tests. Significant evidence for positive selection was identified on genes ST6GALNAC2 and EPHX1 in Yorkshire, PARK2 in Landrace, and BMP6, SLA-DQA1, and PRKG1 in Duroc. These genes are reportedly relevant to lactation, reproduction, meat quality, and growth traits. To understand how these single nucleotide polymorphisms (SNPs) related positive selection affect protein function, we analyzed the effect of non-synonymous SNPs. Three SNPs (rs324509622, rs80931851, and rs80937718) in the SLA-DQA1 gene were significant in the enrichment tests, indicating strong evidence for positive selection in Duroc. Our analyses identified genes under positive selection for lactation, reproduction, and meat-quality and growth traits in Yorkshire, Landrace, and Duroc, respectively.

• Asian-Australasian Journal of Animal Sciences
• /
• v.31 no.8
• /
• pp.1301-1307
• /
• 2018

Objective: This study was conducted to determine the apparent and standardized total tract digestibility (ATTD and STTD) of phosphorus (P) in corn, soybean meal (SBM), and canola meal (CM), and additivity of values for ATTD and STTD of P in corn, SBM, and CM in diets fed to growing pigs. Methods: Thirty-six growing barrows (initial body weight of $21.6{\pm}1.7kg$) were placed in metabolism crates and allotted to a completely randomized design with 6 diets and 6 pigs per diet. Six diets were formulated using corn, SBM or CM as the sole source of P, or corn and SBM, or corn and CM, or corn, SBM, and CM as the P source in each diet, respectively. Fecal samples were collected for 5 d following a 7 d adaptation period to the diets. Results: Values for ATTD and STTD of P in corn, SBM, and CM in growing pigs were 33.12% and 37.76%, 50.19% and 56.62%, 34.93% and 39.45%, respectively. The ATTD and STTD of P in SBM were greater (p<0.05) than those in corn and CM. However, there were no differences in the ATTD or STTD of P between corn and CM. The determined STTD of P in the mixture of corn and SBM, corn and CM, and corn, SBM, and CM is not different from the calculated STTD values. Conclusion: Values for STTD of P in corn, SBM, and CM are additive in their mixture fed to growing pigs.

• The Korean Journal of Mycology
• /
• v.20 no.4
• /
• pp.315-323
• /
• 1992

The effects of several parameters on PCR amplification in using RAPD were studied. The results of this study suggest that approximately 15 ng of genomic DNA in $20\;{\mu}l$ of reaction mixture results in discrete and reproducible PCR products. In addition, the results indicate that concentration or amounts of reaction components studied are highly inter-dependent in their effects, and RNA can interfere severely with PCR amplification. Suitable concentrations or amounts of reaction components were found to be 30 ng of 10-mer primer, $200\;{\mu}M$ of dNTP, 0.001% gelatin 1.5 mM $MgCl_2$, 10 mM Tris-Cl (pH 8.8), 50 mM KCl, 0.1% Triton X-100, 2 units of Taq DNA polymerase, and 15 ng of RNase-treated genomic DNA in $25\;{\mu}l$ of reaction mixture.

• Journal of Microbiology and Biotechnology
• /
• v.10 no.6
• /
• pp.844-850
• /
• 2000

The specific binding and internalization of viral particles is an essential step for the successful infection of viral pathogens. In the case of the hepatitis B virus (HBV), virions bind to the host cell via the preS domain of the viral surface antigen and are subsequently internalized by endocytosis. HBV-preS specific receptors are primarily expressed on hepatocytes, however, viral DNA and proteins have also been detected in extrahepatic sites, suggsting that celluar recepators for HBV may also exist on extrahepatic cells. Recently, an EBV-transformed B-cell line was identified onto which the preS region binds in a receptor-ligand specific manner. In this study, this specific interaction was further characterized, and the binding region within the preS protein was locaized. Also the internalization after host cell attachment was visualized and analyzed by fluorescence-labeled HBV-preS1 proteins using confocal microscopy. Energy depletion by sodium azide treatment effectively inhibited the internalization of the membrane-bound preS1 ligands, thereby indicating an energy-dependent receptor-mediated endocytotic pathway. Accordingly, the interaction of HBV-pres! with this specific B-cell line may serve as an effective model for an infection pathway in extrahepatic cells.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.21 no.12
• /
• pp.663-670
• /
• 2009

Two-phase flow boiling heat transfer of R-410A in horizontal small tubes was reported in the present experimental study. The local heat transfer coefficients were obtained over a heat flux range of 5 to 40 kW/$m^2$ a mass flux range of 170 to 600 kg/$m^2s$, a saturation temperature range of 3 to $10^{\circ}C$, and quality up to 1.0. The test section was made of stainless steel tubes with inner diameters of 0.5 and 3.0 mm, and lengths of 330 and 3000 mm, respectively. The test section was heated uniformly by applying a direct electric current to the tubes. The effects on heat transfer of mass flux, heat flux, inner tube diameter, and saturation temperature were presented. The experimental heat transfer coefficients are compared with six existing heat transfer coefficient correlations. A new boiling heat transfer coefficients correlation based on the superposition model for R-410A in small tubes was developed with mean deviation of 10.13%.