DOI QR코드

DOI QR Code

Characteristics of Particle Separation in Suspension using an Ultrasonic Standing Wave

  • Shin, Beom-Soo (Dept. of Biosystems Engineering, Kangwon National University) ;
  • Danao, Mary-Grace C. (Dept. of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign)
  • 투고 : 2012.03.31
  • 심사 : 2012.04.26
  • 발행 : 2012.04.30

초록

Purpose: Particle separation in solution is one of important process in a unit operation as well as in an extract preparation for biosensors. Contrary to centrifuge-type of mesh-type filter, using an ultrasonic standing wave make the filtering process continuous and free from maintenance. It is needed to investigate the characteristics of particle movement in the ultrasonic standing wave field. Methods: Through the computer simulation the effects of major design and driving parameters on the alignment characteristics of particles were investigated, and a cylindrical chamber with up-stream flow type was devised using two circular-shape PZTs on both sides of the chamber, one for transmitting ultrasonic wave and the other for just reflecting it. Then, the system performance was experimentally investigated as well. Results: The speed of a particle to reach pressure-node plane increased as the acoustic pressure and size of particle increased. The maximum allowable up-stream flow rate could be calculated as well. As expected, exact numbers of pressure-node planes were well formed at specific locations according to the wavelength of ultrasonic wave. As the driving frequency of PZT got close to its resonance frequency, the bands of particles were observed clearer, which meant the particles were trapped into narrower space. Higher excitation voltages to the PZT produced a greater acoustic force with which to trap particles in the pressure-node planes, so that the particles gathered could move upwards without disturbing their alignments even at a higher inlet flow rate. Conclusions: This research showed the feasibility of particle separation in solution in the continuous way by an ultrasonic standing wave. Further study is needed to develop a device to collect or harvest those separated particles.

키워드

참고문헌

  1. Cho, S. H. 2008. Particle separation and control technology using ultrasound. Noise & Vibration 18(5):14-19 (In Korean, with English abstract).
  2. Cho, S. H., D. -C. Seo, B. Ahn, K. -B. Kim and Y. -L. Kim. 2008. Position control of micro particles in a fluid flow using ultrasonic standing wave. Journal of Korean Society for Nondestructive Testing 28(2):131-136 (In Korean, with English abstract).
  3. Cho, S. H., J. H. Park, B. Ahn and K.-B. Kim. 2010. Finite Element Analysis of a Particle Manipulation System Using Ultrasonic Standing Wave. Journal of KSNVE 20(1):3-9 (In Korean, with English abstract). https://doi.org/10.5050/KSNVE.2010.20.1.003
  4. Coakley W. T. 1997. Ultrasonic separations in analytical biotechnology. Trends in Biotechnology 15:506-511. https://doi.org/10.1016/S0167-7799(97)01122-0
  5. Groschl M., W. Burger and B. Handl. 1998. Ultrasonic separation of suspended particles - Part III: Application in Biotechnology. ACUSTICA.acta acustica 84:815-822.
  6. Haake, A and J. Dual. 2002. Micro-manipulation of small particles by node position control of an ultrasonic standing wave. Ultrasonics 40:317-322. https://doi.org/10.1016/S0041-624X(02)00114-2
  7. Hawkes, J.J. and W.T. Coakley. 2001. Force field particle filter, combining ultrasound standing waves and laminar flow. Sensors and Actuators B 75:213-222. https://doi.org/10.1016/S0925-4005(01)00553-6
  8. Johnson, D.A. and D.L. Feke. 1995. Methodology for fractionating suspended particles using ultrasonic standing wave and divided flow fields. Separation Technology 5:251-258. https://doi.org/10.1016/0956-9618(95)00130-1
  9. Kim, E. H., H. K. Cho, K.S. Kyung and G. Kim. 2009. Detection of the Fungicide Iprovalicarb Residues Using a Surface Plasmon Resonance Biosensor. Journal of Biosystems Engineering 34(1):50-56 (In Korean, with English abstract). https://doi.org/10.5307/JBE.2009.34.1.050
  10. Kim, G., G. M. Yang, Y. H. Kim, C. Y. Mo and S. B. Park. 2010. Detection of pathogenic Salmonella with a composite quantum dot. Journal of Biosystems Engineering 35 (6):458-463 (In Korean, with English abstract). https://doi.org/10.5307/JBE.2010.35.6.458
  11. Kim, G., G. Yang, S. B. Park, Y. H. Kim, K. J. Lee, J. Y. Son, H. J. Kim and S. R. Lee. 2011. Rapid detection kit for Salmonella typhimurium. Journal of Biosystems Engineering 36(2):140-146 (In Korean, with English abstract). https://doi.org/10.5307/JBE.2011.36.2.140
  12. Laurell, T., F. Petersson and A. Nilsson. 2007. Chip integrated strategies for acoustic separation and manipulation of cells and particles. Chem. Soc. Rev. 36: 492-506. https://doi.org/10.1039/b601326k
  13. Petersson, F., A. Nilsson, C. Holm, H. Jonsson and T. Laurell. 2004. Separation of lipids from blood utilizing ultrasonic standing waves in microfluidic channels. Analyst 129:938-943. https://doi.org/10.1039/b409139f
  14. Pui, P.W.S., F. Trampler, S.A. Sonderhoff, M. Groschl, D.G. Kilburn and J.M. Piret. 1995. Batch and Semicontinuous Aggregation and Sedimentation of Hybridoma Cells by Acoustic Resonance Fields. Biotechnol. Prog. 11: 146-152. https://doi.org/10.1021/bp00032a005
  15. Tolt T. L. and D. L. Feke. 1993. Separation of dispersed phases from liquids in acoustically driven chambers. Chemical Engineering Science 48(3):527-540. https://doi.org/10.1016/0009-2509(93)80307-C
  16. Townsend R.J., M. Hill, N.R. Harris and N.M. White. 2004. Modelling of particle paths passing through an ultrasonic standing wave. Ultrasonics 42:319-324. https://doi.org/10.1016/j.ultras.2004.01.025
  17. Yasuda K., S. S. Haupt, S. Umemura, T. Yagi, M. Nishida and Y. Shibata. 1997. Using acoustic radiation force as a concentration method for erythrocytes. J. Acoust. Soc. Am. 102(1):642-645. https://doi.org/10.1121/1.421009
  18. Zhou C., P. Pivarnik, A. G. Rand and S. V. Letcher. 1998. Acoustic standing-wave enhancement of a fiber-optic Salmonella biosensor. Biosensors & Bioelectronics 13: 495-500. https://doi.org/10.1016/S0956-5663(97)00141-3

피인용 문헌

  1. Characteristics of Micro-Particle Separation according to HRT Changes vol.35, pp.12, 2013, https://doi.org/10.4491/KSEE.2013.35.12.937