• 물과 미래
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• 제52권9호
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• pp.35-44
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• 2019

• 물과 미래
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• 제52권10호
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• pp.49-58
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• 2019

• 물과 미래
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• 제51권11호
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• pp.13-19
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• 2018

• 물과 미래
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• 제51권12호
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• pp.28-33
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• 2018

• IMMUNE NETWORK
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• 제15권4호
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• pp.199-205
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• 2015

T-bet is a critical transcription factor that regulates differentiation of Th1 cells from $CD4^+$ precursor cells. Since T-bet directly binds to the promoter of the IFN-${\gamma}$ gene and activates its transcription, T-bet deficiency impairs IFN-${\gamma}$ production in Th1 cells. Interestingly, T-bet-deficient Th cells also display substantially augmented the production of IL-2, a T cell growth factor. Exogenous expression of T-bet in T-bet deficient Th cells rescued the IFN-${\gamma}$ production and suppressed IL-2 expression. IFN-${\gamma}$ and IL-2 reciprocally regulate Th cell proliferation following TCR stimulation. Therefore, we examined the effect of T-bet on Th cell proliferation and found that T-bet deficiency significantly enhanced Th cell proliferation under non-skewing, Th1-skewing, and Th2-skewing conditions. By using IFN-${\gamma}$-null mice to eliminate the anti-proliferative effect of IFN-${\gamma}$, T-bet deficiency still enhanced Th cell proliferation under both Th1- and Th2-skewing conditions. Since the anti-proliferative activity of T-bet may be influenced by IL-2 suppression in Th cells, we examined whether T-bet modulates IL-2-independent cell proliferation in a non-T cell population. We demonstrated that T-bet expression induced by ecdysone treatment in human embryonic kidney (HEK) cells increased IFN-${\gamma}$ promoter activity in a dose dependent manner, and sustained T-bet expression considerably decreased cell proliferation in HEK cells. Although the molecular mechanisms underlying anti-proliferative activity of T-bet remain to be elucidated, T-bet may directly suppress cell proliferation in an IFN-${\gamma}$- or an IL-2-independent manner.

• Archives of Pharmacal Research
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• 제18권2호
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• pp.69-74
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• 1995

In order to improve physico-chemical properties and to enhance stability of drugs, amino acid salt has been widely adoptd in pharmaceutical synthesis. Acetylsalicylic acid lysinate is one of the widely used analgesics and it is a good example of t5his synthesis. In the case of bacetylsalicylic acid lysinate synthesis, racemization of natrually occurred lysine is esential because the racemic lysine salt of the drug shows better yield, crystallinity and dryness than that of the L-lysine salt. To esatablish a simple, practical and economical process for L-lysine racemization, L-lysine treatments with phosphoric acid and with acetic acid were compared and the optimum conditions for its process and derivatization were investigated by chiral separation methods using GC_MS spectroscopy.

• Journal of Microbiology and Biotechnology
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• 제33권4호
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• pp.493-499
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• 2023

In this study we evaluated the immune-enhancing effects of β-glucan, the main component of Euglena gracilis (Euglena), and Euglena on inflammatory factor expression in RAW264.7 macrophages and ICR mice with cyclophosphamide-induced immunosuppression. Macrophages were treated with β-glucan or Euglena for 48 h. The β-glucan and Euglena groups exhibited higher levels of inducible nitric oxide synthase, nitric oxide, and tumor necrosis factor (TNF)-α than the control (vehicle alone) group. Animals were fed saline and β-glucan (400 mg/kg body weight (B.W.)) or Euglena (400 or 800 mg/kg B.W.) for 19 days, and on days 17-19, cyclophosphamide (CCP, 80 mg/kg B.W.) was administered to induce immunosuppression in the ICR mouse model. CCP reduced the body weight, spleen index, and cytokine expression of the mice. To measure cytokine and receptor expression, splenocytes were treated with concanavalin A (ConA) or lipopolysaccharide (LPS) as a mitogen for 24 h. In vivo, ConA stimulation significantly upregulated the expression of interferon (IFN)-γ, interleukin (IL)-10, IL-12 receptor β1, IL-1β, and IL-2 in splenocytes from the β-glucan- or Euglena-treated groups compared with those in the splenocytes from the CCP-treated group; LPS stimulation increased the levels of the cytokines TNF-α, IL-1β, and IL-6 in splenocytes from the β-glucan- or Euglena- treated groups compared with those from the CCP-treated group, but most of these differences were not significant. These results demonstrate the effect of Euglena in ameliorating macrophages and immunosuppression in CCP-treated mice. Thus, Euglena has the potential to enhance macrophage- and splenocyte- mediated immune-stimulating responses.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.206.1-206.1
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• 2003

The immunomodulator Ginsan has been found previously by us to stimulate the secretion of high levels of IL -1. IL -6 and TNF-alpha in irradiated mice. These cytokines are known to induce proliferation and differentiation of hematopoietic progenitor cells from the spleen and bone marrow and to protect mice from DNA-damaging agents. The present studies were evaluated as a cytoprotective agent against toxicity of the alkylating drugs. (omitted)

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.810-815
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• 2004

Many biological properties and the clinical potential of human interleukin-2 (hIL-2) draw much attention to its high-level expression in mammalian cells. Recombinant human IL-2 (rhIL-2) was expressed in Chinese hamster ovary (CHO) cells, using the recently developed expression system which confers position-independent expression. Stable CHO cell lines carrying several hundred amplified copies of the rhIL-2 gene were easily obtained and rhIL-2 was expressed at high levels after selection with increasing concentrations of methotrexate. Interestingly, the insertion of the transcription terminator of the human gastrin gene into the downstream region of the gene for rhIL-2 considerably increased rhIL-2 expression. Using the expression system with the transcription terminator, it was possible to get a CHO cell line expressing the rhIL-2 at a very high level, about $11.4\mug/10^6$ cell/day, which is about 6 times higher than that previously reported. The biological activity of the rhIL-2 protein purified from the cell line was also confirmed by the cell proliferation assay.

• 동양고전연구
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• 제62호
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• pp.37-65
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• 2016

본고는 개인적으로 지어진 한문 산문 연행록(燕行錄)이 본격적으로 창작되기 시작한 18세기 전반의 구체적인 창작층과 창작된 산문 연행록의 관심지향 양상을 살피기 위하여 쓰여졌다. 실지적인 작품을 다수 남겼으나, 문헌군 형성 전에 주목받지 못하였던 작자층 중에 '서인 노론 사대신' 중 소재 이이명, 그 아들 일암 이기지, 한포재 이건명의 사적(私的) 한문 산문 연행기록을 연구 대상으로 삼았다. 우선 현전하는 문집에 수록되어 있는 그들의 연행 관련 글을 살핀 결과, 소재와 일암, 한포재의 연행 의식은 '연행의 중요성'을 의식하며 연행의 임무를 경험 대상에 대한 '관찰과 주목'이라는 태도로 실행해야 하는 것으로 설명할 수 있다. 이러한 연행 의식이 구체적으로 그들의 한문 산문 연행기록에서 어떻게 구현되었는가를 살핀 결과는 다음과 같다. 소재 이이명과 일암 이기지 부자의 1720년 사행 결과로 나온 "연행잡지"와 "일암연기"는, 공통적으로 노가재 김창업과 그의 저작 "노가재연행일기"(1712)가 창작의 원인이 되었지만 서술 방법에 있어 화소의 편성을 보면 일암은 천주당을 중심한 서양 문화, 길에서 본 문물의 번성 관찰, 역관 등에게서 들은 현재의 정세 등 자신의 관심사에 따른 직접 체험들의 영역을 편성하고 있으며, 그와 함께 노가재의 교유 인물과 견문을 구비하는 방식을 띤다. 한편 아버지 소재는 자신이 직접 관찰하고 경험한 현상과 일들을 통하여 그의 연행 기록을 작성하는 원칙으로 조선에 영향을 준 청(淸)의 정세, 세법(稅法) 등 제도에 대한 관심지향성을 보인다. 1721년 세제 책봉을 위한 주청사로 간 한포재 이건명의 "한포재 사행일기"에서는, 앞선 두 개의 연행록처럼 자신의 연행 기록을 촉발시킨 사람이나 작품을 거론하지 않지만 서술의 곳곳에서 소재와 일암의 기록, 혹은 전해 들었을 그들의 경험 견문이 반영되어있다. "한포재사행일기"는 등장화소의 성격상 '공무' 와 '사신의 사명'을 중시하고 그 과정을 충실히 소개하고 분석한다는 점에서 소재의 연행기록인 "연행잡지"와 가깝다. 그러나, 자신의 심정 술회를 하기 위해 "일암연기"의 점술 화소를 유입하기도 하고, 공무 외의 견문과 체험에 대해서도 자신이 경험한 것을 간략하게 기록한다. 결국 18세기 전기 한문 산문 연행록의 주 작자층인 서인 노론계 전주 이씨의 작자들은 경험으로서 '사행의 중요성'을 공통적으로 인지한 채로, 자신들이 직접 경험한 영역을 주목하고 관찰하면서 연행록의 내용을 제시하였다. 소재와 한포재는 '공무'와 '사명'의 범주로 특화되며, 일암 이기지는 직접 체험할 수 있었던 타문화의 일상 문물과 교유로까지 넓어진다.