• Parasites, Hosts and Diseases
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• 제28권1호
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• pp.11-24
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• 1990

간흡충의 항원성 부위를 규명하기 위하여 간흡충 충체로부터 분리한 체액을 실험 토끼에 주사하여 면역시킨 IgG와 간흡충에 감염된 실험 토끼의 IgG를 간흡충 조직 항원에 반응시키고 면역황금 표식법을 이용하여 전자현미경으로 관찰한 바 그 결과는 다음과 같다. 간흡충의 조직세포 사이의 구성물인 세포간질과 맹관의 내용물, 그리고 맹관 막 구조물은 충체에 감염된 IgG와 체액 항원에 면역된 IgG 모두에서 항원-항체 반응이 관찰되었지만 체액 항원으로 면역된 IgG에서 다소 강한 반응이 나타났다. 표피의 기저층과 난황선의 난황 물질은 충체에 감염된 IgG에서는 반응이 나타나지 않았으나 체액 항원에 면역된 IgG에서는 강한 반응이 나타나 이들을 구 성하는 물질은 체액 반응 IgG에 대한 특이 항원으로 생각되었다. 배설관의 내용물과 막 구조물은 충체에 감염된 IgG에 대한 반응이 특이하였으나 체액 항원에 면역된 IgG에 대한 반응은 매우 미 약 하여 항원성에 차이점이 있는 것으로 생각되었다. 수정낭의 정자세포 간질은 충체 감염 IgG에 반응 하고 정자세포의 두부는 체액 항원으로 면역된 IgG에 반응하여 매우 상이한 항원성이 나타났다. 이상의 결과로 보아 충체의 세포간질과 맹관 구성물은 충체 감염시와 체액 항원에 면역된 IgG에 대하여 반응의 정도 차이는 있으나 항원성으로 관찰되는 것은 동일하였다. 그러나 수정낭의 세포간질과 배설낭의 구성물질은 충체에 감염된 IgG에 대한 특이 항원이며 수정낭 내의 정자들의 두부와 난황을 구성하는 물질은 체액 면역 IgG에 대한 특이 항원인 것으로 생각된다.

• Journal of Yeungnam Medical Science
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• 제8권1호
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• pp.53-62
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• 1991

인체 혈청에서 추출 정제된 ImmunoglobulinG(IgG)가 임상 병소 분리 녹농균(Ps. aeruginosa) 감염증에 대한 quinolone제제 ciprofloxacin(CPFX)과의 병용 및 단독 투여에 의한 항균효과를 생체감염과 시험관내 실험을 통하여 다음과 같이 요약하였다. 녹농균의 마우스 복강내 감염 후에 마우스 생존율에 의한 항균효과를 IgG 단독 투여군, IgG전 처치후 CPFX 투여군, IgG와 CPFX 동시 투여군 및 CPFX 단독 투여군 순이였다. 녹농균 감염 마우스에서 IgG 단독 투여군에서의 혈액 및 간장내의 생균수는 대조군에 비해 혈액내 균수의 증가는 완만하였으나, 간장내의 균수는 양군 모두 급격한 증가를 나타내었다. PMNL수의 추이는 CPFX 단독 투여군을 제외한 IgG투여군은 감염 후 8시간에 PMNL수가 증가 되었으며, 균 접종 전 IgG투여와 접종 후 IgG투여에 의한 유의적인 차이가 없었다. CPFX의 Ps. aeruginosa에 대한 시험관내 최소 발육 억제 농도는 IgG의 병용 투여에 상관없이 $250{\mu}g/ml$의 높은 내성을 나타내었다. 시험관내에서의 IgG의 직접적 항균작용은 인정되지 않았다.

• Parasites, Hosts and Diseases
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• 제38권3호
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• pp.145-150
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• 2000

Antigenic components in the crude extracts of Spirometra mansoni plerocercoid were analyzed in early experimental infections and in IgG subclass observed in clinical sparganosis. By IgG immunoblot, sera obtained serially from experimental mice, fed 5 spargana each, were reacted with the crude extracts. Protein bands at 36-26 kDa and 103 kDa showed positive reactions since two weeks after infection. In a differential immunoblot, in which a monospecific antibody against sparganum chymase at 36 kDa was pre-treated, the reactions at 36-26 kDa disappeared, indicating that the sparganum chymase and its degradation products invoked IgG antibody reactions. When 69 patients sera of human sparganosis were examined for their IgG subclass responses, IgG4 levels showed the highest reaction which was followed by IgG 1 The IgG4 antibody also reacted mainly with 36-31 kDa protease. These results indicate that 36 kDa chymase of 5. nansoni plerocercoid is the main antigenic component inducing Ige antibody response in early stage of experimental sparganosis and for specific IgG subclass reactions in human sparganosis.

• IMMUNE NETWORK
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• 제12권3호
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• pp.89-95
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• 2012

Immune cells express toll-like receptors (TLRs) and respond to molecular patterns of various pathogens. CpG motif in bacterial DNA activates innate and acquired immune systems through binding to TLR9 of immune cells. Several studies reported that CpG can directly regulate B cell activation, differentiation, and Ig production. However, the role of CpG in B cell growth and Ig production is not fully understood. In this study, we analyzed the effect of CpG on the kinetics of mouse B cell viability, proliferation, and Igs production. Overall, CpG enhanced mouse B cell growth and production of Igs in a dose-dependent manner. Unlike LPS, 100 nM CpG (high dose) did not support TGF-${\beta}1$-induced IgA and IgG2b production. Moreover, 100 nM CpG treatment abrogated either LPS-induced IgM or LPS/TGF-${\beta}1$-induced IgA and IgG2b production, although B cell growth was enhanced by CpG under the same culture conditions. We subsequently found that 10 nM CpG (low dose) is sufficient for B cell growth. Again, 10 nM CpG did not support TGF-${\beta}1$-induced IgA production but, interestingly enough, supported RA-induced IgA production. Further, 10 nM CpG, unlike 100 nM, neither abrogated the LPS/TGF-${\beta}1$- nor the LPS/RA-induced IgA production. Taken together, these results suggest that dose of CpG is critical in B cell growth and Igs production and the optimal dose of CpG cooperates with LPS in B cell activation and differentiation toward Igs production.

• 대한한방소아과학회지
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• 제36권3호
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• pp.35-48
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• 2022

Objectives The purpose of this study is to review relevant clinical studies to investigate the effectiveness of herbal medicines for IgA (Immunoglobulin A) nephropathy in children. Methods Studies on herbal treatment for IgA nephropathy were searched and analyzed through electronic databases such as PubMed, EMBASE, China National Knowledge Infrastructure, Wanfang, CiNii, J-STAGE, Oriental Medicine Advanced Searching Integrated System and Science ON. Studies included Randomized controlled trials and case series. Results We selected nine studies and analyzed the findings. In most studies, improvement of IgA nephropathy was observed after treatment, and was evaluated using factors as 24 hour urine protein, hematuria, Scr (serum creatinine) and BUN (blood urea nitrogen). The most commonly used herbs for IgA nephropathy were Poria cocos (茯苓), Astragalus membranaceus (黃芪), and Rehmannia glutinosa (生地黃). Conclusions Based on the results of the clinical studies, we identified that herbal medicine is an effective treatment for IgA nephropathy. However, additional systematic clinical studies are necessary to prove its effect and safety.

• 한국축산식품학회지
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• 제43권2호
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• pp.346-358
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• 2023

The aim of this study was to evaluate efficacies of selected lactic acid bacteria (LAB) in inducing immunoglobulin A (IgA) secretion. Twenty-five different LAB isolated from traditional fermented Korean foods were characterized for their probiotic properties and screened to identify those that could stimulate lamina propria cells (LPCs) from Peyer's patch to secret IgA in vitro. Among them, four strains (Lactiplantibacillus plantarum CJW55-10, Lactiplantibacillus pentosus CJW18-6, L. pentosus CJW56-11, and Pediococcus acidilactici CJN2696) were found to be strong IgA inducers. The number of IgA positive B cells and soluble IgA level were increased when LPCs were co-cultured with these LAB. Expression levels of toll-like receptor (TLR) such as TLR2 and TLR4 and secretion of interleuckin-6 were augmented in LPCs treated with these LAB. Further, we determined whether oral intake of these LAB enhanced IgA production in vivo. After one-week of daily oral administration, these LAB feed mice increased mucosal IgA and serum IgA. In conclusion, selected strains of LAB could induce systemic IgA secretion by activating lamina propria B cells in Peyer's patch and oral intake of selected strains of LAB can enhance systemic immunity by inducing mucosal IgA secretion.

• Journal of Microbiology and Biotechnology
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• 제13권3호
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• pp.444-450
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• 2003

In order to develop an effective means to treat P. aeruginosa infections, we have purified P. aeruginosa outer membrane proteins (OMPs)-specific human IgG antibody. In this study, we investigated the protective activity of the purified anti-OMPs IgC against P. aeruginosa infection in a rabbit endophthalmitis model. Rabbits were inoculated by an intravitreal injection with P. aeruginosa, and treated with a single dose of 1 mg anti-P. aeruginosa OMPs IgG. All the control rabbits predominantly developed edematous responses and opacity in the eyes, but the rabbits treated with the antibody showed only very limited degree of edema. Aliquots of the vitreous humor were extracted and analyzed for the number of viable bacteria and endotoxin level. The results showed that the anti-OMPs IgC significantly reduced the bacterial count compared with the control group, and that the endotoxin level of the vitreous from the IgG-treated rabbits was more than 70-fold lower 6 h after the administration than the control animals. These data suggested that the anti-P. aeruginosa OMPs IgG is effective in inhibiting the bacterial growth and thereby in reducing endotoxin levels in the vitreous, warranting further development of the anti-P. aeruginosa OMPs IgG as a therapeutic means for treating Pseudomonas endophthalmitis.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제46권1호
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• pp.3-11
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• 2020

Immunoglobulin G4 (IgG4)-related dacryoadenitis and sialoadenitis (IgG4-DS) are part of a multiorgan fibroinflammatory condition of unknown etiology termed IgG4-related disease (IgG4-RD), which has been recognized as a single diagnostic entity for less than 15 years. Histopathologic examination is critical for diagnosis of IgG4-RD. CD4+ T and B cells, including IgG4-expressing plasma cells, constitute the major inflammatory cell populations in IgG4-RD and are thought to cause organ damage and tissue fibrosis. Patients with IgG4-RD who have active, untreated disease exhibit significant increase of IgG4-secreting plasmablasts in the blood. Considerable insight into the immunologic mechanisms of IgG4-RD has been achieved in the last decade using novel molecular biology approaches, including next-generation and single-cell RNA sequencing. Exploring the interactions between CD4+ T cells and B lineage cells is critical for understanding the pathophysiology of IgG4-RD. Establishment of pathogenic T cell clones and identification of antigens specific to these clones constitutes the first steps in determining the pathogenesis of the disease. Herein, the clinical features and mechanistic insights regarding pathogenesis of IgG4-RD were reviewed.

• KSBB Journal
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• 제14권6호
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• pp.677-681
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• 1999

계란의 난황으로부터 유래한 IgY의 분리와 정제과정이 흐름을 정리한 결과, 난황에서의 수용성 단백질의 분리단계가 단백질의 수율을 증가시키는데 가장 큰 영향을 미치는 것으로 판단되었다. 본 연구에서는 자연 침전방법을 통하여 실험하였는데 수율의 증가를 위해서는 원심분리와 같이 수용성 단백질의 양을 많이 얻을 수 있는 방법이 필요하고, 인지질이나 lipoprotein등의 성분의 제거효율을 높이기 위해서는 x-carrageenan등과 같은 natural gum성분의 첨가와 같은 방법이 필요하다. 그러므로 자연침전을 이용하여 수율의 증가측면과 비용의 절감의 효과를 얻을 수 있었다. 보다 효율적인 IgY의 분리를 위해서는 초기 단계에서 수율의 극대화와 불순성분 제거의 최대화가 동시에 요구되고 있다. 전처리 후의 시료를 이용하여 이온교환 크로마토그래피와 gel filtration chromatography를 통해 순도 90% 이상의 IgY를 얻을 수 있었다.

• Restorative Dentistry and Endodontics
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• 제15권2호
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• pp.58-76
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• 1990

This study was designed to elucidate the distribution of the immunoglobulins in the experimentally induced rat periapical lesions. The pulp exposure was performed in 80 molars from 40 rats and the animals were sacrificed at 15, 30, 60 and 90 days after the operation and examined and radiographed. Of the 80 samples, 56 samples were routinely sectioned ($4-6{\mu}$ in thickness) and stained with Hematoxylin-Eosin for the light microscopic examination and 50 samples were stained with toluidin blue for mast cells and 50 samples were stained using the Avidin-Biotin horseradish peroxidase for detecting the presence of Ig A, Ig E, Ig M and Ig G containing cells. The following results were obtained : 1. The periapical lesions could be observed in all of 80 teeth by radiogragh (100%) and the periapical lesions were detected in 50 samples of 51 samples by light microscopy (98%). The size of lesions increased with time lapse both by radiograph and by light microscopy(p<0.05). 2. Of the 50 samples, 19 samples were diagnosed as periapical abscesses, 18 as periapical granulomas, 10 as fibrous scar tissues and 3 cysts. 3. After pulp exposure, periapical granulomas were developed mostly in the 15 day group, with time lapse periapical abscesses and fibrous scar tissues increased. 4. In the 50 periapical lesions, the numbers of Ig G containing cell (57.2%) were prominent and the percentage of Ig A, Ig E and Ig M containing cells were 16.4%, 14.7% and 11.8% respectively. The numbers of all classes of immunoglobulin containing cell were highest in the periapical granulomas and lowest in the cysts(p<0.05). 5. The number of the mast cell and immunoglobulin containing cells decreased generally with time lapse after the pulp exposure and Ig A, Ig E, Ig M and Ig G containing cells and mast cells had the high correlation one another(>0.6).