• 제목/요약/키워드: ITS2 secondary structure

검색결과 191건 처리시간 0.041초

Preparation and characterization of a thermal responsive of poly(N-isopropylacrylamide)/chitosan/gelatin hydrogels

  • Baghaei, Shaghayegh;Khorasani, Mohammad T.
    • Biomaterials and Biomechanics in Bioengineering
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    • 제1권2호
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    • pp.105-116
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    • 2014
  • Synthesis of interpenetrating polymer network (IPN) of chitosan-gelatin (Cs-Ge) (as a primary network) and N-isopropylacrylamide (NIPAAm) monomer (as the secondary network) was carried out with different ratio. Its structure was characterized by FT-IR, which indicated that the IPN was formed. The memberanes were studied by swelling, weight loss with time. The interior morphology of the IPN hydrogels was revealed by scanning electron microscopy (SEM); the IPN hydrogels showed a interpenetrated network of NIPAAm/chitosan has layers with more minute stoma and canals compared to interpenetrated network of NIPAAm/gelatin. Lower critical solution temperature (LCST), equilibrium swelling ratio (ESR) and deswelling kinetics were measured. The DSC results noticed that LCST of IPN hydrogels with different ratio of Cs/Ge/PNIPAAm are around $33{\pm}2^{\circ}C$. The ESR obtained results showed that with a ratio of Cs/Ge/NIPAAm: 1/1/6, the swelling ratio increased drastically from room temperature to $36^{\circ}C$ but with a ratio of Cs/Ge/PNIPAAm: 1/3/6, decrease significantly at the same condition. Therefore the hydrogels have been changed from a hydrophilic structure to a hydrophobic structure. Furthermore with an increase in temperature from room to the LCST, the ESR of IPN with higher concentration of (PNIPAAm) and (Ge) decreases but de-swelling kinetics of them are faster. Due to the suitable and different kinetics of de-swelling and the equilibrium swelling ratio (ESR) in various proportions, and because of the morphology inside the mass which confirms other tests, these hydrogels are very appropriate as a smart thermosensitive hydrogels with rapid response.

히스테리시스 특성을 고려한 자속구속형 고온초전도 사고전류 제한기의 사고전류 제한특성 분석 (Analysis on Fault Current Limiting Characteristics of a Flux-Lock Type HTSC Fault Current Limiter with Hysteresis Characteristic)

  • 임성훈;최명호
    • 조명전기설비학회논문지
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    • 제21권2호
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    • pp.94-98
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    • 2007
  • 자속구속형 초전도 사고전류 제한기는 평상시 자기적인 결합에 의해 철심의 히스테리시스 손실이 발생하지 않는 특징이 있다. 그러나 사고시에는 사고전류증가로 인해 고온초전도 소자의 저항발생과 함께 철심의 포화가 발생할 경우 제한기의 사고전류제한 특성을 저하시키는 현상이 발생하게 된다. 따라서 본 논문에서는 이를 분석하고 억제하기 위한 설계방안을 도출하기 위해 자속구속형 고온초전도 사고전류 제한기의 구성요소인 자속구속 리액터의 히스테리시스 특성을 고려한 사고전류 제한특성을 분석하였다. 이를 위해 제한기의 등가회로와 사고전류 제한실험으로부터 히스테리시스 곡선을 도출하였다. 1, 2차권선의 인덕턴스 비에 따른 히스테리시스 곡선으로부터 인덕턴스비가 증가함에 따라 제한되는 사고전류크기는 증가한 반면 사고시 자속구속형 사고전류제한기의 구성요소인 철심의 포화가 억제됨을 확인할 수 있었다.

ITS에 의한 한국내 마가목 속 분류군의 유전적 계통분류학적 연구 (Phylogenetic Study of Genus Sorbus in Korea by Internal Transcribed Spacer Sequence (ITS))

  • 허만규;김세현;박소혜
    • 생명과학회지
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    • 제17권12호
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    • pp.1610-1615
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    • 2007
  • 마가목 속(genus Sorbus)은 목본류로 아시아와 유럽에 분포되어 있다. 마가목 속 식물은 한국과 중국에서 약용으로 쓰인다. 한국내 마가목 속 식물에 대해 ITS에 의한 계통관계를 조사하였다 마가목 속 전체 종에서 5.8S exon은 165 핵산서열이었다. ITS1은 유럽마가목(S. aucuparia)에서 218 핵산서열인 반면 한국 내 마가목 종은 219 핵산서열이었다. ITS2 핵산서 열은 다양하였는데 S. sambucifolia var. pseudogricilisto에서 는 240 핵산서 열로 가장 적은 반면 S. aucuparia에서는 245 핵산서열이었다. ITS 전체 서열은 625개로 35개는 절약법에 정보적이었다. ITS 서 열로 한국내 분류군과 유럽종간 구분이 잘 되었다. RNA 2차 구조 추정 분석에서 도메인 I과 II는 마가목 속에 잘 보전되어 있는 반면 도메인 III에서는 많은 차이를 나타내었다. ITS 서열로 종 동정에 이용할 수 있었으며, 종의 보전이나 생식질 보전에 기초로 이용될 수 있을 것으로 사료된다.

Bioactivation of Aromatic Amines by Human CYP2W1, An Orphan Cytochrome P450 Enzyme

  • Eun, Chang-Yong;Han, Song-Hee;Lim, Young-Ran;Park, Hyoung-Goo;Han, Jung-Soo;Cho, Kyoung-Sang;Chun, Young-Jin;Kim, Dong-Hak
    • Toxicological Research
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    • 제26권3호
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    • pp.171-175
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    • 2010
  • The human genome contains approximately 13 orphan cytochrome P450 (P450, CYP) genes, of which the apparent function or substrate has not been identified. However, they seem to possess their own biological relevance in some tissues or developmental stages. Here, we characterized the heterologously expressed CYP2W1, an orphan P450 enzyme. The recombinant CYP2W1 protein containing a $6{\times}$(His)-tag at Nterminus has been expressed in Escherichia coli and purified. Expression level of CYP2W1 holoenzyme was around 500 nmol P450 holoenzyme per liter culture medium. The reduced CO difference spectrum of CYP2W1 showed a maximum absorption at 449 nm. CYP2W1 indicated the significant induction to bioactivate Trp-P-1, MeIQ, and IQ in E. coli DJ701 tester strain. However, the bioactivation of B[$\alpha$]P, and NNK by CYP2W1 was relatively low. The model structure of CYP2W1 suggested the characteristic P450 folds with the lengths and orientations of the individual secondary elements. The F-G loop is situated on the distal side of heme to accommodate the flexibility of active site of CYP2W1. These studies can provide useful information for the finding of its biological roles and structure-function relationships of an orphan CYP2W1 enzyme.

Purification of Caudal-Related Homeodomain Transcription Factor and Its Binding Characterization

  • Jeong, Mi-Suk;Hwang, Eun-Young;Kim, Hyun-Tae;Yoo, Mi-Ae;Jang, Se-Bok
    • Journal of Microbiology and Biotechnology
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    • 제19권12호
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    • pp.1557-1564
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    • 2009
  • Human CDX2 is known as a caudal-related homeodomain transcription factor that is expressed in the intestinal epithelium and is important in differentiation and maintenance of the intestinal epithelial cells. The caudal-related homeobox proteins bind DNA according to a helix-turn-helix structure, thereby increasing the structural stability of DNA. A cancer-tumor suppressor role for Cdx2 has been shown by a decrease in the level of the expression of Cdx2 in colorectal cancer, but the mechanism of transcriptional regulation has not been examined at the molecular level. We developed a large-scale system for expression of the recombinant, novel CDX2, in Escherichia coli. A highly purified and soluble CDX2 protein was obtained in E. coli strain BL21(DE3)RIL and a hexahistidine fusion system using Ni-NTA affinity column, anion exchange, and gel filtration chromatographies. The identity and secondary structure of the purified CDX2 protein were confirmed by MALDI-TOF MS, Western blot, and a circular dichroism analyses. In addition, we studied the DNA-binding activity of recombinant CDX2 by ELISA experiment and isolated human CDX2-binding proteins derived from rat cells by an immobilized GST-fusion method. Three CDX2-binding proteins were found in the gastric tissue, and those proteins were identified to the homeobox protein Hox-D8, LIM homeobox protein 6, and SMC1L1 protein.

산업관련표(産業關聯表)에 의(依)한 임업구조분석(林業構造分析)과 유발생산액(誘發生産額) -임업(林業)이 한국경제(韓國經濟)에 미치는 영향(影響)- (Analysis of Forestry Structure and Induced Output Based on Input - output Table - Influences of Forestry Production on Korean Economy -)

  • 이승윤
    • Journal of the Korean Wood Science and Technology
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    • 제2권4호
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    • pp.4-14
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    • 1974
  • The total forest land area in Korea accounts for some 67 percent of the nation's land total. Its productivity, however, is very low. Consequently, forest production accounts for only about 2 percent of the gross national product and a minor proportion of no more than about 5 percent versus primary industry. In this case, however, only the direct income from forestry is taken into account, making no reference to the forestry output induced by other industrial sectors. The value added Or the induced forestry output in manufacturing the primary wood products into higher quality products, makes a larger contribution to the economy than direct contribution. So, this author has tried to analyze the structure of forestry and compute the repercussion effect and the induced output of primary forest products when utilized by other industries for their raw materials, Hsing the input-output table and attached tables for 1963 and 1966 issued by the Bank of Korea. 1. Analysis of forestry structure A. Changes in total output Durng the nine-year period, 1961-1969, the real gross national product in Korea increased 2.1 times, while that of primary industries went up about 1. 4 times. Forestry which was valued at 9,380 million won in 1961, was picked up about 2. 1 times to 20, 120 million won in 1969. The rate of the forestry income in the GNP, accordingly, was no more than 1.5 percent both in 1961 and 1962, whereas its rate in primary industries increased 3.5 to 5.4 percent. Such increase in forestry income is attributable to increased forest production and rise in timber prices. The rate of forestry income, nonetheless, was on the decrease on a gradual basis. B. Changes in input coefficient The input coefficient which indicates the inputs of the forest products into other sectors were up in general in 1966 over 1963. It is noted that the input coefficient indicating the amount of forest products supplied to such industries closely related with forestry as lumber and plywood, and wood products and furniture, showed a downward trend for the period 1963-1966. On the other hand, the forest input into other sectors was generally on the increase. Meanwhile, the input coefficient representing the yolume of the forest products supplied to the forestry sector itself showed an upward tendency, which meant more and more decrease in input from other sectors. Generally speaking, in direct proportion to the higher input coefficient in any industrial sector, the reinput coefficient which denotes the use of its products by the same sector becomes higher and higher. C. Changes in ratio of intermediate input The intermediate input ratio showing the dependency on raw materials went up to 15.43 percent m 1966 from 11. 37 percent in 1963. The dependency of forestry on raw materials was no more than 15.43 percent, accounting for a high 83.57 percent of value added. If the intermediate input ratio increases in any given sector, the input coefficient which represents the fe-use of its products by the same sector becomes large. D. Changes in the ratio of intermediate demand The ratio of the intermediate demand represents the characteristics of the intermediary production in each industry, the intermediate demand ratio in forestry which accunted for 69.7 percent in 1963 went up to 75.2 percent in 1966. In other words, forestry is a remarkable industry in that there is characteristics of the intermediary production. E. Changes in import coefficient The import coefficient which denotes the relation between the production activities and imports, recorded at 4.4 percent in 1963, decreased to 2.4 percent in 1966. The ratio of import to total output is not so high. F. Changes in market composition of imported goods One of the major imported goods in the forestry sector is lumber. The import value increased by 60 percent to 667 million won in 1966 from 407 million won in 1963. The sales of imported forest products to two major outlets-lumber and plywood, and wood products and furniture-increased to 343 million won and 31 million won in 1966 from 240million won and 30 million won in 1963 respectively. On the other hand, imported goods valued at 66 million won were sold to the paper products sector in 1963; however, no supply to this sector was recorded in 1963. Besides these major markets, primary industries such as the fishery, coal and agriculture sectors purchase materials from forestry. 2. Analysis of repercussion effect on production The repercussion effect of final demand in any given sector upon the expansion of the production of other sectors was analyzed, using the inverse matrix coefficient tables attached to the the I.O. Table. A. Changes in intra-sector transaction value of inverse matrix coefficient. The intra-sector transaction value of an inverse matrix coefficient represents the extent of an induced increase in the production of self-support products of the same sector, when it is generated directly and indirectly by one unit of final demand in any given sector. The intra-sector transaction value of the forestry sector rose from 1.04 in 1963 to 1, 11 in 1966. It may well be said, therefore, that forestry induces much more self-supporting products in the production of one unit of final demand for forest products. B. Changes in column total of inverse matrix coefficient It should be noted that the column total indicates the degree of effect of the output of the corresponding and related sectors generated by one unit of final demand in each sector. No changes in the column total of the forestry sector were recorded between the 1963 and 1966 figures, both being the same 1. 19. C. Changes in difference between column total and intra-sector transaction amount. The difference between the column total and intra-sector transaction amount by sector reveals the extent of effect of output of related industrial sector induced indirectly by one unit of final demand in corresponding sector. This change in forestry dropped remarkable to 0.08 in 1966 from 0.15 in 1963. Accordingly, the effect of inducement of indirect output of other forestry-related sectors has decreased; this is a really natural phenomenon, as compared with an increasing input coefficient generated by the re-use of forest products by the forestry sector. 3. Induced output of forestry A. Forest products, wood in particular, are supplied to other industries as their raw materials, increasng their value added. In this connection the primary dependency rate on forestry for 1963 and 1966 was compared, i. e., an increase or decrease in each sector, from 7.71 percent in 1963 to 11.91 percent in 1966 in agriculture, 10.32 to 6.11 in fishery, 16.24 to 19.90 in mining, 0.76 to 0.70 in the manufacturing sector and 2.79 to 4.77 percent in the construction sector. Generally speaking, on the average the dependency on forestry during the period 1963-1966 increased from 5.92 percent to 8.03 percent. Accordingly, it may easily be known that the primary forestry output induced by primary and secondary industries increased from 16, 109 million won in 1963 to 48, 842 million won in 1966. B. The forest products are supplied to other industries as their raw materials. The products are processed further into higher quality products. thus indirectly increasing the value of the forest products. The ratio of the increased value added or the secondary dependency on forestry for 1963 and 1966 showed an increase or decrease, from 5.98 percent to 7.87 percent in agriculture, 9.06 to 5.74 in fishery, 13.56 to 15.81 in mining, 0.68 to 0.61 in the manufacturing sector and 2.71 to 4.54 in the construction sector. The average ratio in this connection increased from 4.69 percent to 5.60 percent. In the meantime, the secondary forestry output induced by primary and secondary industries rose from 12,779 million Wall in 1963 to 34,084 million won in 1966. C. The dependency of tertiary industries on forestry showed very minor ratios of 0.46 percent and 0.04 percent in 1963 and 1966 respectively. The forestry output induced by tertiary industry also decreased from 685 million won to 123 million won during the same period. D. Generally speaking, the ratio of dependency on forestry increased from 17.68 percent in 1963 to 24.28 percent in 1966 in primary industries, from 4.69 percent to 5.70 percent in secondary industries, while, as mentioned above, the ratio in the case of tertiary industry decreased from 0.46 to 0.04 percent during the period 1963-66. The mining industry reveals the heaviest rate of dependency on forestry with 29.80 percent in 1963 and 35.71 percent in 1966. As it result, the direct forestry income, valued at 8,172 million won in 1963, shot up to 22,724 million won in 1966. Its composition ratio lo the national income rose from 1.9 percent in 1963 to 2.3 per cent in 1966. If the induced outcome is taken into account, the total forestry production which was estimated at 37,744 million won in 1963 picked up to 105,773 million won in 1966, about 4.5 times its direct income. It is further noted that the ratio of the gross forestry product to the gross national product. rose significantly from 8.8 percent in 1963 to 10.7 percent in 1966. E. In computing the above mentioned ratio not taken into consideration were such intangible, indirect effects as the drought and flood prevention, check of soil run-off, watershed and land conservation, improvement of the people's recreational and emotional living, and maintenance and increase in the national health and sanitation. F. In conclusion, I would like to emphasize that the forestry sector exercices an important effect upon the national economy and that the effect of induced forestry output is greater than its direct income.

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여수해역에서 채집한 보름달 둥근 물해파리의 핵과 미토콘드리아 DNA 염기서열을 이용한 유연 관계 분석 (Molecular phylogeny of moon jellyfish Aurelia aurita Linnaeus collected from Yeosu waters in Korea based on nuclear and mitochondrial DNA sequences)

  • 김숙양;조은섭
    • 생명과학회지
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    • 제17권3호통권83호
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    • pp.318-327
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    • 2007
  • 본 연구는 여수연안해역에서 채 집 한 보름달 둥근 물해파리를 대상으로 ITS 부위와 미토콘드리아 유전자 염기서열을 이용하여 계통유연관계를 보왔다. ITS 부위를 증폭시키 기 위하여 F5와 R5 primer, 미토콘드리아 COI 유전자 증폭을 위하여 LCO1490과 HCO2198 primer를 사용했다. 증폭은 ITS에서 267 bp, COI에서 643 bp로 나타났다. 한국산 물해파리와 미국 캘리포니아에서 채집한 Aurelia sp.가 유전적 거리가 가장 짧은 0.023을 보인 반면에, 한국산과 미국산, 스웨덴산 물해파리는 동일한 종이지만 유전적 거리가 0.393에서 0.395로 매우 먼 것으로 나타났다. COI유전자의 경우 한국산과 영국산, 터어키산, 스웨덴산, 미국산 물해파리의 유전적 거리 범위는 0.201에서 0.205로 나타났다. 그러나 한국산과 미국산의 bootstrap은 100% 자매군으로 보였다. COI 유전자에 대한 한국산과 미국산 2차 RNA folding 구조를 볼 때 동일한 에너지 하에서도 상이한 2차 folding을 보였다. 따라서 ITS1과 COI 유전자는 보름달 둥근 물해파리 개체군의 생물지리학적 분포 조사를 위하여 유용한 도구로 활용될 것으로 추측된다.

Close Relationship Between SARS-Coronavirus and Group 2 Coronavirus

  • Kim, Ok-Ju;Lee, Dong-Hun;Lee, Chan-Hee
    • Journal of Microbiology
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    • 제44권1호
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    • pp.83-91
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    • 2006
  • The sudden appearance and potential lethality of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) in humans has resulted in a focusing of new attention on the determination of both its origins and evolution. The relationship existing between SARS-CoV and other groups of coronaviruses was determined via analyses of phylogenetic trees and comparative genomic analyses of the coronavirus genes: polymerase (Orflab), spike (S), envelope (E), membrane (M) and nucleocapsid (N). Although the coronaviruses are traditionally classed into 3 groups, with SARS-CoV forming a $4^{th}$ group, the phylogenetic position and origins of SARS-CoV remain a matter of some controversy. Thus, we conducted extensive phylogeneitc analyses of the genes common to all coronavirus groups, using the Neighbor-joining, Maximum-likelihood, and Bayesian methods. Our data evidenced largely identical topology for all of the obtained phylogenetic trees, thus supporting the hypothesis that the relationship existing between SARS-CoV and group 2 coronavirus is a monophyletic one. Additional comparative genomic studies, including sequence similarity and protein secondary structure analyses, suggested that SARS-Co V may bear a closer relationship with group 2 than with the other coronavirus groups. Although our data strongly suggest that group 2 coronaviruses are most closely related with SARS-CoV, further and more detailed analyses may provide us with an increased amount of information regarding the origins and evolution of the coronaviruses, most notably SARS-CoV.

Immunoinformatics studies and design of a novel multi-epitope peptide vaccine against Toxoplasma gondii based on calcium-dependent protein kinases antigens through an in-silico analysis

  • Ali Dalir Ghaffari;Fardin Rahimi
    • Clinical and Experimental Vaccine Research
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    • 제13권2호
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    • pp.146-154
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    • 2024
  • Purpose: Infection by the intracellular apicomplexan parasite Toxoplasma gondii has serious clinical consequences in humans and veterinarians around the world. Although about a third of the world's population is infected with T. gondii, there is still no effective vaccine against this disease. The aim of this study was to develop and evaluate a multimeric vaccine against T. gondii using the proteins calcium-dependent protein kinase (CDPK)1, CDPK2, CDPK3, and CDPK5. Materials and Methods: Top-ranked major histocompatibility complex (MHC)-I and MHC-II binding as well as shared, immunodominant linear B-cell epitopes were predicted and linked using appropriate linkers. Moreover, the 50S ribosomal protein L7/L12 (adjuvant) was mixed with the construct's N-terminal to increase the immunogenicity. Then, the vaccine's physicochemical characteristics, antigenicity, allergenicity, secondary and tertiary structure were predicted. Results: The finally-engineered chimeric vaccine had a length of 680 amino acids with a molecular weight of 74.66 kDa. Analyses of immunogenicity, allergenicity, and multiple physiochemical parameters indicated that the constructed vaccine candidate was soluble, non-allergenic, and immunogenic, making it compatible with humans and hence, a potentially viable and safe vaccine candidate against T. gondii parasite. Conclusion: In silico, the vaccine construct was able to trigger primary immune responses. However, further laboratory studies are needed to confirm its effectiveness and safety.

인터페이스 구조에 따른 프로토타입의 반응형 웹 (Responsive Web of Prototype based on Implementation Structure)

  • 장승영
    • 한국전자통신학회논문지
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    • 제13권3호
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    • pp.609-616
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    • 2018
  • 본 논문은 인터페이스 구조를 근거로 프로토콜타입의 웹 사이트 설계 방식에 대한 반응형 웹의 설계에 필요한 기술들을 두고 서술하게 되었다. 반응형 설계는 프로토콜 타입의 요구사항을 인터페이스 기반사항에 근거로 분석하여 개발과정에 적용하는 프로세서를 의미한다. 개발에 있어서 우선원칙에 의한 설계방식을 수립하고 디자인공식에 의한 유연성과 가변성을 설정하여 사용자중심의 제1원칙과 제2원칙으로 나누어 인터페이스 구조로 정립해 나갔다. 그리고 압축기술을 논의를 통해 비동기적 스크립트를 결합방식의 방법을 모색하여 웹의 프로토콜 타입에 맞게 확장시킬 수 있는 방법을 찾게 되었다. 오늘날 웹 사이트나 웹 애플리케이션에 대한 필요성은 장치에 관계없이 적응하고 접근할 수 있어야 한다. 이러한 해결방안으로 반응형 웹 사이트 설계방식에서 찾게 되었으며 반응형 웹 사이트 설계방식에 대한 인터페이스 구조와 이를 근거로 한 프로토콜 타입의 웹 사이트 설계방식에 대한 중요기법들을 마련하게 되었다.