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http://dx.doi.org/10.5352/JLS.2007.17.12.1610

Phylogenetic Study of Genus Sorbus in Korea by Internal Transcribed Spacer Sequence (ITS)  

Huh, Man-Kyu (Department of Molecular Biology, Dongeui University)
Kim, Sea-Hyun (Biotechnology Division of Forest Genetics, Korea Forest Research Institute)
Park, So-Hye (Department of Molecular Biology, Dongeui University)
Publication Information
Journal of Life Science / v.17, no.12, 2007 , pp. 1610-1615 More about this Journal
Abstract
Genus Sorbus is a long lived woody species that is primarily distributed throughout Asia and Europe. This species is regarded as very important herbal medicines in Korea and China. Sorbus commixta is primarily distributed throughout Europe. We evaluated a representative sample of the four taxa with nuclear ribosomal DNA internal transcribed spacer sequences (ITS) to estimate genetic relationships within genus. Aligned nucleotide sequences of the length of ITS1 were nearly constant within genus Sorbus varying from 219 in S. aucuparia to 218 in the rest species. Especially, the 5.8S subunit of all taxa of Sorbus was found to constant of 165 bp nucleotides. However, aligned nucleotide sequences of the length of ITS2 vary from 240 in S. sambucifolia var. pseudogrcilisto 245 in S. aucuparia. Total alignment length is 629 positions, of which 35 are parsimony-informative, 32 variable but parsimony-uninformative, and 552 constant characters. The base furtherance showed the difference to the by a total taxon: an average A and T are 17.7% and G and C are 30.4%, 34.2%, respectively. All the four taxa beginning with conserved base paired triplets emerging from single strand regions (domain I). Noteworthy, in the RNA secondary structure proposed for the three Korean Sorbus taxa RNA transcript ITS2, which shows a remarkedly well-conserved folding (domain II). When compared to the European Sorbus (S. aucuparia) of ITS2. ITS analysis may be useful in germ-plasm classification several taxa of genus Sorbus.
Keywords
Sorbus; ITS; phylogenetic analysis;
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