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http://dx.doi.org/10.5352/JLS.2007.17.3.318

Molecular phylogeny of moon jellyfish Aurelia aurita Linnaeus collected from Yeosu waters in Korea based on nuclear and mitochondrial DNA sequences  

Kim, Sook-Yang (South Sea Fisheries Research Institute, NFRDI)
Cho, Eun-Seob (South Sea Fisheries Research Institute, NFRDI)
Publication Information
Journal of Life Science / v.17, no.3, 2007 , pp. 318-327 More about this Journal
Abstract
This study presents the molecular phylogenetic analysis of Korean Aurelia aurita Linnaeus collected from Yeosu in the southern waters of Korea using nuclear ITS1 region and mitochondrial COI gene sequences. The use of oligonucleotide primers F5 (forward) and R5 (reverse) targeted to ITS1 and LCO1490 (forward) and HCO2198 (reverse) targeted to COI amplified 267 bp and 643 bp fragments, respectively. The shortest genetic distance towards the ITS1 region is estimated at 0.023 when comparing Korean A. aurita to Aurelia sp. collected from California, USA. In particular, Korean and American/Swedish A. aurita were located far away in terms of genetic distance, ranging from 0.393 to 0.395. On the other hand, the genetic distance between Korean and English/Turkish/Swedish/American A. aurita regarding the mitochondrial DNA COI gene ranged from 0.201 to 0.205. However, a sister-ship with Korean and American A. aurita showed an extremely high bootstrap value (100%). The predicted secondary RNA structure of the mitochondrial DNA COI gene showed many different folding structures with a similar energy between Korean and American A. aurita. These results suggest that ITS1 and the mitochondrial DNA COI gene could be used as genetic markers for identification of the biogeographic populations.
Keywords
aquaculture; ark shell; gene flow; geographic characteristic; mitochondrial DNA; population structure;
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