• Journal of Electrochemical Science and Technology
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• 제12권2호
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• pp.246-256
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• 2021

Mo,Cu-doped CeO₂ (CMCuO) nanopowders were synthesized by the nitrate-fuel combustion method aiming to improve the electrical and electrochemical properties of its Mo-doped CeO₂ (CMO) parent by the addition of copper. An electrical conductivity of ca. 1.22·10^-2 S cm^-1 was measured in air at 800℃ for CMCuO, which is nearly 10 times higher than that reported for CMO. This increase was associated with the inclusion of copper into the crystal lattice of ceria and the presence of Cu and Cu₂O as secondary phases in the CMCuO structure, which also could explain the increase in the charge transfer activities of the CMCuO based anode for the hydrogen and carbon monoxide electro-oxidation processes compared to the CMO based anode. A maximum power density of ca. 120 mW cm^-2 was measured using a CMCuO based anode in a solid oxide fuel cell (SOFC) with YSZ electrolyte and LSM-YSZ cathode operating at 800℃ with humidified syngas as fuel, which is comparable to the power output reported for other SOFCs with anodes containing copper. An increase in the area specific resistance of the SOFC was observed after ca. 10 hours of operation under cycling open circuit voltage and polarization conditions, which was attributed to the anode delamination caused by the reduction of the Cu₂O secondary phase contained in its microstructure. Therefore, the addition of a more electroactive phase for hydrogen oxidation is suggested to confer long-term stability to the CMCuO based anode.

• Biomolecules & Therapeutics
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• 제25권2호
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• pp.171-176
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• 2017

Streptomyces avermitilis produces clinically useful drugs such as avermectins and oligomycins. Its genome contains approximately 33 cytochrome P450 genes and they seem to play important roles in the biosynthesis of many secondary metabolites. The SAV_7130 gene from S. avermitilis encodes CYP158A3. The amino acid sequence of this enzyme has high similarity with that of CYP158A2, a biflaviolin synthase from S. coelicolor A3(2). Recombinant S. avermitilis CYP158A3 was heterologously expressed and purified. It exhibited the typical P450 Soret peak at 447 nm in the reduced CO-bound form. Type I binding spectral changes were observed when CYP158A3 was titrated with myristic acid; however, no oxidative product was formed. An analog of flaviolin, 2-hydroxynaphthoquinone (2-OH NQ) displayed similar type I binding upon titration with purified CYP158A3. It underwent an enzymatic reaction forming dimerized product. A homology model of CYP158A3 was superimposed with the structure of CYP158A2, and the majority of structural elements aligned. These results suggest that CYP158A3 might be an orthologue of biflaviolin synthase, catalyzing C-C coupling reactions during pigment biosynthesis in S. avermitilis.

• Journal of Microbiology and Biotechnology
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• 제28권12호
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• pp.2106-2112
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• 2018

Concanavalin A (ConA) interacts with carbohydrates as a lectin, and recent reports proposed its application for detecting a diversity of viruses and pathogens. Structural studies have detailed the interaction between ConA and carbohydrates and the metal coordination environment with manganese and calcium ions (Mn-Ca-ConA). In this study, ConA was crystallized with a cadmium-containing precipitant, and the refined structure indicates that $Mn^{2+}$ was replaced by $Cd^{2+}$ (Cd-Ca-ConA). The structural comparison with ConA demonstrates that the metal-coordinated residues of Cd-Ca-ConA, that is Glu8, Asp10, Asn14, Asp19, and His24, do not have conformational shifts, but residues for sugar binding, including Arg228, Tyr100, and Leu99, reorient their side chains, slightly. Previous studies demonstrated that excess cadmium ions can coordinate with other residues, including Glu87 and Glu183, which were not coordinated with $Cd^{2+}$ in this study. The trimeric ConA in this study coordinated $Cd^{2+}$ with other residues, including Asp80 and Asp82, for complex generation. The monomer does not have specific interaction near interface regions with the other monomer, but secondary cadmium coordinated with two aspartates (Asp80 and Asp82) from monomer 1 and one aspartate (Asp16) from monomer 2. This study demonstrated that complex generation was induced via coordination with secondary $Cd^{2+}$ and showed the application potential regarding the design of complex formation for specific interactions with target saccharides.

• 한국자기공명학회논문지
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• 제20권2호
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• pp.36-40
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• 2016

MRA1997 is a highly conserved protein from mycobacterial strains. However, no structural and functional information is associated with it. Thus, to obtain details about structure and function of this protein, we have utilized NMR spectroscopy. The recombinant MRA1997 was highly purified and its DNA binding mode was characterized. The tertiary structure of MRA1997 was modeled on the basis of our NMR chemical shift data combined with the webserver CS23D. The binding of MRA1997 with DNA was first monitored by electrophoresis mobility shift assays. The residues involved in DNA binding are identified using NMR chemical shift perturbation experiments. Based on our study, we suggest that MRA1997 interacts with DNA and may play an important role in Mycobacterium tuberculosis physiology.

• 한국세라믹학회지
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• 제43권2호
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• pp.121-125
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• 2006

[ $LiFePO_4$ ] is one of the promising materials for cathode material of secondary lithium batteries due to its high energy density, low cost, environmental friendliness and safety. $LiFePO_4$ was synthesized by the solid-state reaction method at 500 - 800°C. The crystal structure of $LiFePO_4$ was analyzed by X-ray powder diffraction. The samples synthesized at 600 and $700^{\circ}C$ showed a single phase of a olivine structure. The particle sizes were increased and the specific surface areas were decreased with heating temperatures. The electrochemical performance was investigated by coin cell test. The discharge capacities at 0.1 C-rate were 118 mAh/g and 112 mAh/g at $600^{\circ}C,\;700^{\circ}C$, respectively. In an attempt to improve the electrical conductivity of cathode materials, $LiFePO_4/graphite$ composite was prepared with various graphite contents. The electrical conductivity and discharge capacity were increased with increasing the graphite contents in composite samples. The rate capabilities at high current densities were also improved.

• 대한수의학회지
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• 제33권1호
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• pp.23-36
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• 1993

To investigate the cycle and relative frequences and the fine structure of seminiferous epithelia in mature Jindo dogs, histologic study was performed. The results obtained were summarized as follows; 1. Type A spermatogonia appeared approximately 1.6 times as many at stage II as compared to stage I while type In spermatogonia appeared small amount in stage III, IV and V. type B spermatogonia were found during the stage VI to VIII, though not detectable during stage I to V. The type B spermatogonia divided at stage VII to produce the preleptotene primary spermatocytes at stage VIII. The number of primary spermatocytes of the leptotene phase markedly increased during stage I to II, and the primary spermatocytes of the pachytene phase were shown the least in number at stage IV. The secondary spermatocytes could be seen only at stage IV. 2. The relative frequencies of each stage from stages I to VIII of the cycle of seminiferous epithelia were 31.6, 11.9, 10.0, 3.2, 8.2, 10.1, 11.7 and 13.2% respectively. 3. On electron microscopic observations, acrosomal vesicle of spermatids appeared larger though the bulk of germ cells were the morphologically same as those of the other animal species. Thread line structures light microscopically observed in the cytoplasm of Sertoli cell were the longitudinal orientation of mitochondria.

• 대한토목학회논문집
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• 제26권5B호
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• pp.469-479
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• 2006

본 연구에서는 사행수로에서 주 흐름과 이차류의 특성을 정량적으로 분석하고자 중심각이 $150^{\circ}$이고 사행도 1.52인 S자형 사행수로에서 실험을 수행하였다. 평균수심과 유량을 달리하여 다양한 실험 조건 하에서 수행한 실험을 통해 다중 만곡부를 갖는 사행수로에서 이차류의 공간적 변화양상을 관찰하였다. 실험결과, 주 흐름은 실험조건에 관계없이 직선구간에서 좌우 대칭적인 유속분포를 보였고, 만곡부에서는 내안쪽에 최대유속이 발생하고 외안쪽에 최소유속이 발생하는 현상을 발견 할 수 있었다. 이렇게 주 흐름이 최단노선을 따라 발생하는 현상은 기존 연구자들의 결과와 일치하는 것이다. 이차류의 거동은 첫번째 만곡부보다 두 번째 만곡부에서 더욱 활발하게 발달하고 외안 회전류가 뚜렷히 나타남을 발견하였다. 이차류의 강도는 직선구간에서는 낮게 나타나고 만곡부에서는 증가하는 주기적인 현상을 보였으며, 만곡부의 이차류 강도가 직선구간보다 2~3배 크게 나타났다. 또한, 두 번째 만곡부에서 이차류 강도의 최대값이 발생했다. 주 흐름방향의 난류 강도와 Reynolds 전단응력을 분석한 결과, 주 흐름 난류 강도는 주 흐름의 유속편차가 클수록 증가하는 것으로 나타났고 Reynolds 전단응력은 주 흐름방향 유속의 편차가 크게 벌어지는 동시에 이차류가 활발히 생성되는 지점에서 크게 나타나는 경향을 보였다.

• Journal of the Korean Physical Society
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• 제73권10호
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• pp.1535-1540
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• 2018

In this study, we prepared and characterized Nb-doped $Li_7La_3Zr_{2-x}O_{12}$ (LLZNO) powder and pellets with a cubic garnet structure by using a modified sol-gel synthesis and hot pressing. LLZNO powder with a very small grain size and cubic structure without secondary phases could be obtained by using a synthesis method in which Li and La sources in a propanol solvent were mixed together with Zr and Nb sources in 2-methoxy ethanol. A pure cubic phase LLZNO pellet could be fabricated from the prepared LLZNO and an additional 6-wt% of $Li_2CO_3$ powder by hot pressing at $1050^{\circ}C$ and 15.8 MPa. The hot-pressed LLZNO pellet with a relative density of 99% exhibited a very dense surface morphology. The total Li ionic conductivity of the hot-pressed LLZNO was $7.4{\times}10^{-4}S/cm$ at room temperature, which is very high level compared to other reported values. The activation energy for ionic conduction was estimated to be 0.40 eV.

• 한국환경교육학회지:환경교육
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• 제16권2호
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• pp.39-50
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• 2003

Secondary school environmental education is offered mostly in extra-curricular activities, not included in the school curriculum. Therefore, drawing out its curriculum has been left to teachers' discretion. Teachers' beliefs in EE have much more influence on the selection of content of EE than any other psychological factors. It follows that the reality of school EE depends on what teachers believes about EE and which content of EE they select according to their beliefs. This study is to research the realities of the secondary school EE: examine teachers' beliefs about EE and analyse environmental views in their environment-related programs; examine whether teachers' programs reflect their beliefs and environmental views; identify the factors which teachers inhibit teachers from their expression of these beliefs. To identify what perspective was reflected to these programs, this study employs environmentalism. Environmentalism can be classified into 3 categories: environmental management; deep ecology; social ecology. Data were gathered by means of in-depth interviews with five teachers. To show teachers' beliefs clearly, the analysis of the data was conducted on the basis of 5 categories: 'ecological sensitivity', 'value education', 'behaviors', 'issues', 'perception of a cause of environmental problems, related to social structures'. These belifs reflect deep ecological perspective and social ecology. But these perspectives did not accord with those in programs. Most EE programs included only the deep ecological perspective. They didn't reflect their beliefs that students should perceive the cause of environmental problems in the social structure. The factors which teachers find as inhibiting or supporting their expression of these beliefs were analysed as following: (1) EE requests the change of curriculum and methods of instruction; (2) teachers should acquire 'environmental consciousness' and environmentrelated knowledge; (3) programs requests an administrative and financial support.

• Structural Monitoring and Maintenance
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• 제2권2호
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• pp.95-113
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• 2015

The knowledge of the behavior of any roller compacted concrete (RCC) dam and its foundation is gained by studying the service action of the dam and its foundation using measurements of an external and internal nature. The information by which a continuing assurance of structural safety of the RCC dam can be gauged is of primary importance. Similarly, the fact that the information on structural and thermal behavior and the properties of concrete that may be used to give added criteria for use in the design of future RCC dams is of secondary importance. Wide spread attention is now being given to the installation of more expensive instrumentation for studying the behavior of concrete dams and reservoirs and forecasting of any adverse trends. In view of this, the paper traces installation and need of the comprehensive instrumentation scheme implemented to monitor the structural and thermal behavior of 102.4 m high RCC dam constructed near Mumbai in India. An attempt is made in the present paper to emphasize the need to undertake an instrumentation program and evaluate their performance during construction and post construction stage of RCC structures. Few typical results, regarding the thermal and structural behavior of the dam, obtained through instrumentation installed at the dam site are presented and compared with the design considerations. The fair agreement is seen in the response observed through instrumentation with that governing the design criteria.