• 한국정보처리학회논문지
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• 제7권5호
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• pp.1474-1481
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• 2000

The number of keystream cycle sequences has been proposed as a characteristic of binary sequence generator for cryptographic application, but in general the most of binary sequence generators have a single cycle. On the other hand, S-box has been used to block cipher for a highly nonlinear element and then we apply it to the stream cipher with a high crypto-degree. In this paper, we propose a multiple-cycle binary sequence generator based on S-box which has a high nonlinearity containing SAC property and analyze its period, linear complexity, randomness and the number of keystream cycle sequences.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2000년도 International Symposium on Bioinformatics
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• pp.69-70
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• 2000

The completion of sequencing human genome would motivate us to map millions of human cDNAs onto the unique ruler "genome sequence", in order to identify the exact address of each cDNA together with its exons, its promoter region, and its alternative splicing patterns. The expression patterns of some cDNAs could therefore be associated with these precise gene addresses, which further accelerate studies on mining correlations between motifs of promoters and expressions of genes in tissues. Towards the realization of this goal, we have developed a time-and-space efficient software named SQUALL that is able to map one cDNA sequence of length a few thousand onto a long genome sequence of length thirty million in a couple of minutes on average. Using SQUALL, we have mapped twenty thousand of our Bodymap (http://bodymap.ims.u-tokyo.ac.jp) cDNAs onto the genome sequences of Chr.21st and 22nd. In this talk, I will report the status of this ongoing project.

• 한국균학회지
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• 제49권1호
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• pp.11-19
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• 2021

In 2017, Talaromyces variabilis was isolated during a survey of fungal diversity in field soils in Korea. This isolate was described based on its morphological and molecular characteristics and it was identified molecularly using the partial 18S-ITS1-5.8S-ITS2-28S rDNA region and calmodulin (CaM)-encoding gene sequence data. Thus, this study reported morphological and multigene sequence characterization of T. variabilis.

• 생명과학회지
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• 제26권11호
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• pp.1341-1344
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• 2016

보길도에서 자라고 있는 황칠나무(Dendropanax morbifera)를 구입하여, 캘러스로 유도한 후, ribosomal DNA(nrDNA)의 internal transcribed spacer (ITS) region의 염기서열을 결정하였다 보길도의 황칠나무(Dendropanax morbifera)의 ITS region의 염기서열을 분석한 결과, 총 689염기를 결정하였다. 결정된 689염기 중에서 ITS1은 222 개염기, 5.8S rDNA는 160염기, ITS2는 233염기인 것으로 판명되었다. GenBank의 BLAST 프로그램(http://www.ncbi.nlm.nih.BLAST)을 사용하여 GenBank/EMBL/DDBJ에 등록되어 있는 Dendropanax 속 33의 염기서열을 수집한 후 multiple alignment를 수행한 결과, 유사도는 99.7%(D. chevalieri)에서 92.6%(Dendropanax arboreus)로 나타났으며, 일본황칠나무(D. trifidus)와는 유사도가 99.4%로 판명되었다.

• Journal of Microbiology and Biotechnology
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• 제1권1호
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• pp.45-49
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• 1991

The complete nucleotide sequence of alkalophilic Bacillus sp. K-17 $\beta$-xylosidase gene and its flanking regions were established. A 1263-bp of an open reading frame for $\beta$-xylosidase was observed. The molecular weight (50, 521 dalton), deduced from the nucleotide sequence of $\beta$-xylosidase gene, agreed with the result obtained by SDS-polyacrylamide gel electrophoresis of the purified enzyme (51, 000 dalton). The Shine-Dalgarno sequence, 5'-GAGGAGG-3', was found 8 bp upstream of the initiation codon ATG. The -10 sequence (TAAAAT) in the promoter region for $\beta$-xylosidase gene was similar to the consensus sequence for Bacillus subtilis RNA polymerase, whereas the -35 sequence (TCGATCA) different from all the known -35 regions in the promoter for Bacillus subtilis RNA polymerase.

• 조명전기설비학회논문지
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• 제24권5호
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• pp.55-61
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• 2010

PLC 제어 시스템은 SFC 언어를 사용하여 설계할 경우, SFC 언어를 사용하면 제어의 흐름을 이해하기 쉽고, 유지보수가 용이하며 프로그램의 기술성이 뛰어나다. SFC 언어는 단일 시퀀스, 선택 시퀀스, 병렬 시퀀스로 나누어지며, 선택 시퀀스로 프로그래밍 하면 단일 시퀀스로 프로그램할 때보다 메모리의 크기가 커져야 한다. 본 논문에서는 선택 시퀀스의 기능을 단일 시퀀스로 구현하여 메모리의 크기를 줄여서 메모리의 효율을 향상시키는 방법을 제시하고, 실례를 통해 타당성을 확인하였다.

• BMB Reports
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• 제37권2호
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• pp.144-147
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• 2004

Molecular amplification and sequencing of genomic DNA that encodes camel polyubiquitin (PUBC1) was performed by a polymerase chain reaction (PCR) using various sets of primers. The amplification generated a number of DNA fragments, which were sequenced and compared with the polyubiquitin coding sequences of various species. One DNA fragment that conformed to 325 bp was found to be 95 and 88% homologous to the sequences of human polyubiquitin B and C, respectively. The DNA translated into 108 amino acids that corresponded to two fused units of ubiquitin with no intervening sequence, which indicates that it is a polyubiquitin and contains at least two units of ubiquitin. Although, variations were found in the nucleotide sequence when compared to those of other species, the amino acid sequence was 100% homologous to the polyubiquitin sequences of humans, mice, and rats. This is the first report of the polyubiquitin DNA coding sequence and its corresponding amino acid sequence from camels, amplified using direct genomic DNA preparations.

• 대한의생명과학회지
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• 제24권4호
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• pp.430-434
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• 2018

Candida glabrata is the second most prevalent causative agent for candidiasis following C. albicans. The opportunistic yeast, C. glabrata, is able to cause the critical bloodstream infections in hospitalized patients. Conventional identification methods for yeasts are often time consuming and labor intensive. Therefore, recent studies on sequence-based identification have been conducted. Recently, sequencing the D1/D2 domain of the large subunit ribosomal RNA gene and the internal transcribed spacers (ITS) 1 and ITS2 regions of the ribosomal DNA has proven useful for DNA-based identification of most species of fungi. In the present study, therefore, fungal ITS and D1/D2 domain regions were targeted and analyzed by DNA sequencing for the accurate identification of C. glabrata clinical isolates. A total of 102 C. glabrata clinical isolates from various clinical samples including bloodstream, catheterized urine, bile and other body fluids were used in the study. The results of the DNA sequence analysis showed that the mean standard deviation of species identity percent score between ITS and D1/D2 domain regions was $97.8%{\pm}2.9$ and $99.7%{\pm}0.46$, respectively. These results revealed that the D1/D2 domain region might be a better target for identifying C. glabrata clinical isolates based on DNA sequences than the ITS1 and ITS2 regions. However, in order to evaluate the usefulness of D1/D2 domain region for species identification of all Candida species, other Candida species such as C. albicans, C. tropicalis, C. dubliniensis, and C. krusei should be verified in further studies additionally.

• 한국자원식물학회지
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• 제19권5호
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• pp.628-633
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• 2006

태백제비꽃군내 식물들은 동소적으로 생육하면서 단엽에서 장상복엽까지 연속적인 중간형을 나타내어 분류학적 어려움이 있다. 본 연구의 목적은 태백제비꽃, 단풍제비꽃, 남산제비꽃 그리고 각 분류군 사이의 중간형을 잎의 형태에 따라 5 집단으로 구분하고 각 집단별 대표적인 개체를 선별하여 ITS DNA 염기서열을 분석하고 분류학적 어려움을 해결하는데 있다. 정렬된 ITS1, ITS2 그리고 5.8S 지역의 염기서열은 702 bp로 나타났다. 5.8S 지역은 163 bp로 조사된 모든 개체에서 변이가 없었으며, ITS1과 ITS2는 일부 변이가 있어 분산분석, 염기 서열 분기 조사 그리고 분계분석에 이용하였다. 분산분석 결과 조사된 잎 형태별 개체들 간에 차이가 없는 것으로 나타났다. 염기서열 분기 조사 결과, 군외군으로 설정한 낚시제비꽃과 노랑제비꽃의 경우 Kimura 2-parameter distance에서 절대치가 0.05보다 훨씬 높게 나타나서 뚜렷한 차이가 확인되었다. 그러나 군내군 5 개체는 절대치가 모두 매우 낮게 나타나서 염기서열 분기는 종 수준이 아닌 종 이하의 수준으로 판단되었다. 분계분석에서 군외군으로 설정한 2 종은 기저 분계조를 형성하였다. 군내군은 하나의 분계조를 형성하였지만, 부트스트랩이 50% 이하로 나타나 계통학적 의미는 적은 것으로 사료된다.

• The Plant Pathology Journal
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• 제26권1호
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• pp.83-89
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• 2010

Taxonomic position of 46 Korean isolates of Rhizoctonia solani which were classified into nine intraspecific groups by anastomosis and cultural characteristics was analyzed by randomly amplified polymorphic DNA (RAPD) and sequence analyses of the internal transcribed spacer (ITS) regions of ribosomal DNA. All the isolates within each group showed highly similar band patterns in RAPD. The ITS regions of the isolates within the same groups showed a high level of sequence similarity above 96.0% whereas similarities among different groups were below 94.4%. When compared with several reference strains of R. solani from foreign countries, all the Korean isolates were clustered with the foreign isolates belonging to the same groups in the phylogenetic tree. All six Korean strains of AG-4 were identified as HG-1 out of 3 subgroup of AG-4. We discussed taxonomic position of Korean isolates of R. solani and showed that sequence analysis with ITS regions could be a rapid and useful method for identification of intraspecific group of R. solani.