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http://dx.doi.org/10.5423/PPJ.2010.26.1.083

Taxonomic Position of Korean Isolates of Rhizoctonia solani Based on RAPD and ITS Sequencing of Ribosomal DNA  

Jeon, Young-Ah (National Academy of Agricultural Science, RDA)
Kim, Wan-Gyu (Korean Agricultural Culture Collection of National Agrobiodiversity Center and Agricultural Microbiology Division)
Kim, Dae-Ho (National Academy of Agricultural Science, RDA)
Kwon, Soon-Wo (National Academy of Agricultural Science, RDA)
Hong, Seung-Beom (National Academy of Agricultural Science, RDA)
Publication Information
The Plant Pathology Journal / v.26, no.1, 2010 , pp. 83-89 More about this Journal
Abstract
Taxonomic position of 46 Korean isolates of Rhizoctonia solani which were classified into nine intraspecific groups by anastomosis and cultural characteristics was analyzed by randomly amplified polymorphic DNA (RAPD) and sequence analyses of the internal transcribed spacer (ITS) regions of ribosomal DNA. All the isolates within each group showed highly similar band patterns in RAPD. The ITS regions of the isolates within the same groups showed a high level of sequence similarity above 96.0% whereas similarities among different groups were below 94.4%. When compared with several reference strains of R. solani from foreign countries, all the Korean isolates were clustered with the foreign isolates belonging to the same groups in the phylogenetic tree. All six Korean strains of AG-4 were identified as HG-1 out of 3 subgroup of AG-4. We discussed taxonomic position of Korean isolates of R. solani and showed that sequence analysis with ITS regions could be a rapid and useful method for identification of intraspecific group of R. solani.
Keywords
anastomosis; ITS; randomly amplified polymorphic DNA; Rhizoctonia solani;
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1 Vilgalys, R. and Gonzales, D. 1990. Ribosomal DNA restriction fragment length polymorphisms in Rhizoctonia solani. Phytopathology 80:151-158.   DOI
2 White, T. J., Bruns, T. D., Lee, S. and Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A guide to methods and applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sinsky and T. J. White, pp. 315-322. Academic Press, New York, USA.
3 Reynolds, M., Weinhold, A. R. and Moris, T. J. 1983. Comparison of anastomosis groups of Rhizoctonia solani by polyacrylamide gel electophoresis of soluble proteins. Phytopathology 73:903-906.   DOI
4 Salazar, O., Schneider, J. H. M., Julian, M. C., Keijer, J. and Rubio, V. 1999. Phylogenetic subgrouping of Rhizoctonia solani AG 2 isolates based on ribosomal ITS sequences. Mycologia 91:459-467.   DOI
5 Sharma, M., Gupta, S. K. and Sharma, T. R. 2005. Characterization of variability in Rhizoctonia solani by using morphological and molecular markers. J. Phytopathol. 153:449-456.   DOI   ScienceOn
6 Stevens Johnk, J., Jones, R. K., Shew, H. D. and Carling, D. E. 1993. Characterization of populations of Rhizoctonia solani AG-3 from potato and tobacco. Phytopathol. 83:854-858.   DOI
7 Sneh, B., Burpee, L. and Ogoshi, A. 1991. Identification of Rhizoctonia species. APS Press, St. Paul, MN., USA. pp. 133.
8 Stevens Johnk, J. and Jones, R. K. 2001. Differentiation of three homogeneous groups of Rhizoctonia solani anastomosis group 4 by analysis of fatty acids. Phytopathology 91:821-830.   DOI   ScienceOn
9 Stevens Johnk, J. and Jones, R. K. 1993. Differentiation of populations of AG-2-2 of Rhizoctonia solani by analysis of fatty acids. Phytopathology 83:278-283.   DOI
10 Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596-1599.   DOI   ScienceOn
11 Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucl. Acids Res. 22:4673-4680.   DOI
12 Kuninaga, S., Natsuaki, T., Takeuchi, T. and Yokosawa, R. 1997. Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani, Curr. Genet. 32:237-243.   DOI
13 Kuninaga, S., Carling, D. E., Takeuchi, T. and Yokosawa, R. 2000. Comparison of rDNA-ITS sequences between potato and tobacco strains in Rhizoctonia solani AG-3. J. Gen. Plant Pathol. 66:2-11.   DOI
14 Kuninaga, S. and Yokosawa, R. 1980. A comparison of DNA base compositions among anastomosis groups in Rhizoctonia solani Khn. Ann. Phytopathol. Sco. Jpn. 46:150-158.   DOI
15 O'Brien, P. A. 1994. Molecular markers in Australian isolate of Rhizoctonia solani. Mycol. Res. 98:665-671.   DOI
16 Kuninaga, S. and Yokosawa, R. 1984. DNA base sequence homology in Rhizoctonia solani Khn. IV. Genetic relatedness within AG-4. Ann. Phytopathol. Sco. Jpn. 50:322-330.   DOI
17 Liu, Z. L. and Sinclair, J. B. 1992. Genetic diversity of Rhizoctonia solani anastomosis group 2. Phytopathology 82:778-787.   DOI
18 Liu, Z. L. and Ge, Q. X. 1988. Studies on soluble protein patterns of Rhizoctonia solani by polyacrylamide gel electrophoresis. I. The influence of different source of isolates on protein patterns of anastomosis group. Acta Mycol. Sin. 7:175-182.
19 Ogoshi, A. and Ui, T. 1979. Specific vitamin requirement among anastomosis groups of Rhizoctonia solani Khn. Ann Phytopathol. Soc. Jpn. 45:47-53.   DOI
20 Ogoshi, A. 1987. Ecology and pathogenicity of anastomosis and intraspecific groups of Rhizoctonia solani Khn. Annu. Rev. Phytopathol. 25:125-143.   DOI   ScienceOn
21 Pope, E. J. and Carter, D. A. 2001. Phylogenetic placement and host specificity of mycirrhizal isolates belonging to AG-6 and AG-12 in the Rhizoctonia solani species complex. Mycologia 93:712-719.   DOI
22 Carling, D. E. and Leiner, R. H. 1990. Virulence of isolates of Rhizoctonia solani AG-3 collected from potato plant organs and soil. Plant Dis. 74:901-903.   DOI
23 Carling, D. E., Kuninaga, S. and Brainard, K. A. 2002. Hyphal anastomosis reactions, rDNA-internal transcribed spacer sequences, and virulence levels among subsets of Rhizoctonia solani AG 2 and AG BI. Phytopathology 92:43-50.   DOI   ScienceOn
24 Hong, S. B., Go, S. J., Ryu, J. C., Kim, W. G. and Kim, I. S. 1998. Differentiation of intraspecific groups within Korean isolates of Rhizoctonia solani using PCR-RFLP of ribosomal DNA. Kor. J. Plant Pathol. 14:157-163 (In Korean).
25 Date, H., Yagi, S., Okamoto, Y. and Onili, N. 1984. On the leaf blight of tomatoes Thanatephorus cucumeris (Frank) Donk (Rhizoctonia solani). Ann. Phytopathol. Soc. Jpn. 50:339.   DOI
26 Gonzalez, D., Carling, E. D., Kuninaga, S., Vilgalys, R. and Cubeta, M. A. 2001. Riobosomal DNA sysmatics of Ceratobasidium and Thanatephorus with Rhizoctonia anamorphs. Mycologia 93:1138-1150.   DOI
27 Guleria, S., Aggarwal, R., Thind, T. S. and Sharma, T. R. 2007. Morphological and pathological variability in rice isolates of Rhizoctonia solani and molecular analysis of their genetic variability. J. Phytopathol. 155:654-661.   DOI   ScienceOn
28 Kang, H. W., Park, D. S. and Eun, M. Y. 2002. Fingerprinting of diverse genomes using PCR with universal rice primers generated from repetitive sequence of Korean weedy rice. Mol. Cells 13:281-287.
29 Kim, W. G., Cho, W. D. and Lee, Y. H. 1994. Anastomosis groups and cultural characteristics of Rhizoctonia solani isolates from crops in Korea. Kor. J. Mycol. 22:309-324.   과학기술학회마을
30 Kim, W. G., Cho, W. D. and Ryu, H. Y. 1995. Diagnosis and control of Rhizoctonia diseases on crops. Research Report RDA.
31 Carling, D. E. 1996. Grouping in Rhizoctonia solani by hyphal anastomosis reaction. In: Sneh et al. (eds) Rhizoctonia species: taxonomy, molecular biology, ecology, pathology and disease control. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp. 37-47.
32 Adams, G. C. Jr. and Butler, E. E. 1979. Serological relationships among anastomosis groups of Rhizoctonia solani. Phytopathology 69:629-633.   DOI
33 Anderson, N. A. 1982. The genetics and pathology of Rhizoctonia solani. Ann. Rev. Phytopathol. 20:329-347.   DOI   ScienceOn
34 Boysen, M., Borja, M., del Moral, C., Salazar, O. and Rubio, V. 1996. Identification at strain level of Rhizoctonia solani AG-4 isolates by direct sequence of asymmetric PCR products of the ITS regions. Curr. Genet. 29:174-181.   DOI