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Morphological and Multigene Sequence Characteristics of Talaromyces variabilis Isolated from Soil in Korea

  • Adhikari, Mahesh (Division of Biological Resource Sciences, Kangwon National University) ;
  • Kim, Sang Woo (Division of Biological Resource Sciences, Kangwon National University) ;
  • Lee, Hyang Burm (Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lee, Youn Su (Division of Biological Resource Sciences, Kangwon National University)
  • Received : 2020.09.29
  • Accepted : 2021.03.08
  • Published : 2021.03.31

Abstract

In 2017, Talaromyces variabilis was isolated during a survey of fungal diversity in field soils in Korea. This isolate was described based on its morphological and molecular characteristics and it was identified molecularly using the partial 18S-ITS1-5.8S-ITS2-28S rDNA region and calmodulin (CaM)-encoding gene sequence data. Thus, this study reported morphological and multigene sequence characterization of T. variabilis.

Keywords

Acknowledgement

This study was financed by the Ministry of Environment (MOE) of the Republic of Korea from the National Institute of Biological Resources (NIBR) grant (NIBR2014-01205) on the survey and discovery of indigenous fungal species in Korea. This study has been worked with the support of a research grant of Kangwon National University in 2019.

References

  1. Adhikari M, Yadav DR, Kim SW, Um YH, Kim HS, Lee HB, Lee YS. Discovery of two new Talaromyces species from crop field soil in Korea. Mycobiol 2015;43:402-7. https://doi.org/10.5941/MYCO.2015.43.4.402
  2. Kirk PM, Cannon PF, David JC, Stalpers JA. Ainsworth and Bisby's dictionary of the fungi. 9th ed. Wallingford: CABI Publishers; 2001.
  3. Visage CM, Yilmaz N, Frisvad JC, Houbraken J, Seifart KA, Samson RA, Jacobs K. Five new Talaromyces species with ampuliform-like phialides and globose rough walled conidia resembling T. verruculosus. Mycoscience 2015;56:486-502. https://doi.org/10.1016/j.myc.2015.02.005
  4. Benjamin CR. Ascocarps of Aspergillus and Penicillium. Mycologia 1955;47:669-87. https://doi.org/10.2307/3755578
  5. Antipova TV, Zhelifonova VP, Kochkina GA, Kozlovskii AG. Growth and biosynthesis of rugulovasines in Penicillium variable. Microbiol 2008;444:446-50.
  6. Davet P, Rouxel F. Detection and isolation of soil fungi. Enfield (NH): Science Publishers; 2000.
  7. Samson RA, Houbraken J, Thrane U, Frisvad JC, Andersen B. Food and indoor fungi. CBS laboratory manual series 2. Utrecht: CBS-KNAW Fungal Biodiversity Centre; 2010.
  8. Kornerup A, Wanscher JH. Methuen handbook of color. 2nd ed. Copenhagen: Sankt Jorgen Tryk; 1967.
  9. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: A guide to methods and applications. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. San Diego (CA): Academic Press; 1990. p. 315-22.
  10. National Center for Biotechnology Information. GenBank overview [Internet]. Bethesda (MD): National Center for Biotechnology Information; 2015 [cited 2019 March 5]. Available from: https://www.ncbi.nlm.nih.gov/genbank/.
  11. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  12. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111-20. https://doi.org/10.1007/BF01731581
  13. Penicillium variabile Sopp, Skrifter udgivne af Videnskabs-Selskabet i Christiania. Mathematisk-Naturvidenskabelig Klasse. 1912;11:169.
  14. Tsang CC, Tang JYM, Lau SKP, Woo PCY. Taxonomy and evolution of Aspergillus, Penicillium and Talaromyces in the omics era - Past, present and future. Comput Struct Biotechnol J 2018;16:197-210. https://doi.org/10.1016/j.csbj.2018.05.003
  15. Berbee ML, Yoshimura A, Sugiyama J, Taylor JW. Is Penicillium monophyletic? An evaluation of phylogeny in the family Trichocomaceae from 18S, 5.8S and ITS ribosomal DNA sequence data. Mycologia 1995;87:210-22. https://doi.org/10.2307/3760907
  16. Skouboe P, Taylor JW, Frisvad JC, Lauritsen D, Larsen L, Alboek C, Boysen M, Rossen L. Molecular methods for differentiation of closely related Penicillium species. Integration of Modern Taxonomic Methods for Penicillium and Aspergillus Classification. In: Samson RA, Pitt JI, editors. Amsterdam: Harwood Academic Publishers; 2000.
  17. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W. Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci USA 2012;109:6241-6. https://doi.org/10.1073/pnas.1117018109
  18. Geiser DM, Harbinski FM, Taylor JW. Molecular and analytical tools for characterizing Aspergillus and Penicillium species at the intra- and interspecific levels. Integration of modern taxonomic methods for Penicillium and Aspergillus classification. In: Samson RAP, editor. Amsterdam: Harwood Acadmic Publishers; 2000. p. 381-94.
  19. Halo BA, Ai-Yahai RA, Maharachikumbura SSN, Ai-Sadi A. Talaromyces variabilis interferes with Pythium aphanidermatum growth and suppresses Pythium-induced damping-off of cucumbers and tomatoes. Scientific Reports 2019;9:11255. https://doi.org/10.1038/s41598-019-47736-x