• Title/Summary/Keyword: ITS region

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The Function of Multiple Pribnow Box on the Aerobic-Anaerobic Switch Control of aeg-46.5 Gene Expression

  • Gang, In O;Jeong, Yeon Ju;Choe, Mu Hyeon
    • Bulletin of the Korean Chemical Society
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    • v.22 no.8
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    • pp.903-908
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    • 2001
  • The gene aeg-46.5, which is expressed under anaerobic condition, has putative triple -10 regions and four transcription start sites. The mRNA transcription level and its start point change depending on the aerobic/anaerobic growth condition. RNA polymerase and its regulatory proteins must choose which of three -10 region to use. The putative triple 10 region was mutated to make only one of them function with consensus -10 region sequence (TATAAT) and the other two as non-functional region. The results show that the second and third -10 regions are used for the aerobic/anaerobic expression. The third -10 region is responsible for the high aerobic to anaerobic switch ratio. This suggests that only the last two of the putative triple -10 region have functions on aeg-46.5 gene expression switch control. The phenotype of the mutated promoter was tested in the wild type cell and narL - cell. The results indicate that the control by NarL is independent from the selection of -10 region. The expression patterns on multi-copy plasmids and on single-copy chromosome were compared. These results show that the aerobic/anaerobic switch control of aeg-46.5 is through the choice of -10 region. The mechanism of choosing different -10 region remains to be seen.

Millimeter-wave Technologies for ITS Wireless Communications

  • Fujise, Masayuki
    • Information and Communications Magazine
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    • v.19 no.9
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    • pp.31-42
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    • 2002
  • CRL (Communications Research Laboratory, Independent Administrative Institution, Japan) is developing ITS wireless communication technologies in millimeter-wave frequency region. Technologies are categorized for inter-vehicle communications (IVC) and road-vehicle communications (RVC). In this paper, system concepts and the experimental facilities are introduced. The experimental facility for inter-vehicle communications is gotten ready in 60 GHz frequency region and the experimental system for road-vehicle communications is based on RoF (Radio on Fiber) technology in 36-37GHz frequency region.

Leaf variants of Pinus and their ITS DNA sequences (소나무속 잎 변이와 그의 ITS DNA 염기서열)

  • Koo, JaChoon;Whang, Sung Soo
    • Korean Journal of Plant Taxonomy
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    • v.43 no.1
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    • pp.63-68
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    • 2013
  • ITS DNA sequences of two variants of Pinus spp. having single fasciculate leaf or two to three fasciculate leaves within an individual were analysed in order to determine their origin. Also, the same DNA locus of P. densiflora, P. rigida and P. koraiensis, distributed at the same region together with the OTUs having leaf variations, were analysed to compare with each other. Aligned sequences including ITS1, 5.8S and ITS2 were 580~584 bp in length. The 5.8S region was well conserved among all the OTUs we tested except for P. koraiensis, which has two nucleotide substitutions. The partial ITS1 region upstream of the 5.8S region showed relatively high sequence diversity compare to the ITS2 and has 181~185 bp in length. In this region, the sequences of the two variants were identical to that of P. densiflora. The ITS2 has identical for all OTUs in length and the two variants also have same sequences compare to that of P. densiflora. These two variants and samples of P. densiflora were grouped together in the UPGMA tree with 100% similarity level. The result strongly suggest that these two variants were originated from P. densiflora.

An Experimental Study on the Depth Variation of Water Flow on Steep Open Channel with Constant Width (一定幅 急傾斜 開水路上을 流動하는 물의 깊이 變化에 관한 實驗的 硏究)

  • 박이동
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.10 no.1
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    • pp.86-95
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    • 1986
  • A study on film water flow on steep open channel has been seldom found up to date. Therefore, this paper dealed with the depth variation of film thickness of water (city supply normal water) flowing on steep open channel. For this purpose, Experimental apparatus (made of a normal glass with 160cm of length and 15cm of width) was made and the depths of the water flowing on the channel were measured experimentally, changing the channel slope angle from 30 to 80 degree (5 steps) and the flow rate from 0.25 to 10CPM (11 steps). The results obtained, some characteristics of the film flow on the channel are as follows. (1) When thin film water flowed on steep open channel, the depths of flow tended to increase after decreasing and was kept nearly constant in its downstream in case of laminar and transitional flow region. The turining point of the depths of flow from decrease to increase tended to move downward with the increase of Reynolds number. In turbulent flow region, the depths of flow showed reapid decrease in its upper stream, gradual decrease in its midstream and were kept nearly constant in its downstream. (2) While the differences between the depths of flow along the channel slope got small in its upper stream and got large in its downstream in case of laminar flow region, they got very large in its upper stream and were kept nearly constant in its downstream in case of transitional and turbulent flow region. And the move flow rate increases, the more the differences between the depths of flow along the channel slope got large in its upper stream.

Distribution of Polycyclic Aromatic Hydrocarbons in PM$_{10}$ and its Adjacent Soil of Urban Atmosphere (도시대기의 미세먼지(PM$_{10}$) 및 주변토양중 다환방향족 탄화수소의 농도분포에 관한 연구)

  • 박기학;최성훈
    • Journal of Environmental Health Sciences
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    • v.24 no.2
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    • pp.1-8
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    • 1998
  • This study was carried out to investigate the distribution of PAHs in atmosphere and soil, and their relationship, accumulation tendency in soil, and its contain burden ratio (%) the PM$_{10}$ and soil were sampled adjacent to a roadside and analysed by HPLC from August 25th 1996 to September 22nd 1996 in Seoul and Kunggido area. The main results are summarized below 1. The concentration of PM$_{10}$ in the ambient air was showed in order of industrial region ($142.70\pm 21.77 \mu g/m^3$), commercial region ($136.51\pm 31.62 \mu g/m^3$), residential region ($110.12\pm 14.98 \mu g/m^3$), greenbelt region ($77.44\pm 12.12 \mu g/m^3$), respectively. 2. Distribution of PAHs concentration level in PM$_{10}$ and soil was showed in order of industrial region, commercial region, residential region, greenbelt region, respectively in all components. 3. The contain burden ratio (%) in PM$_{10}$ was attested that BbF was the highest contain burden component (21.7-32%) and An was the lowest contain burden component (0.35-1.95%) in all region. 4. The contain burden ratio (%) in soil was attested that Pb (20.5%) was the highest and An (0.8%) was the lowest contain burden component in industrial region and BbF (21.9%) and An (0.45%) were the same tendency in commercial region and Py (21.6%) and BkF (3.5%) were in residential region, Py (29%) and An (2.6%) were in greenbelt region. 5. The relationship between concentration of PAHs in PM$_{10}$ and soil was attested that the component of BbF (r=0.514) was very highly correlated, and there were significant in Fl and BaA in soil between industrial region and commercial region (P<0.05).

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Cloning, Sequencing and Characterization of Mitochondrial Control Region of the Domestic Silkwom, Bombyx mori

  • Lee, Jin-Sung;Kim, Ki-Hwan;Hoe, Hyang-Sook;Park, Jae-Heung;Kang, Seok-Woo;Lee, Sang-Han;Hwang, Jae-Sam
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.1
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    • pp.87-89
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    • 2001
  • The nucleotide sequence of the domestic silkworm (Bombyx mori) mitochondrial (mt) control region and its flanking genes was determined from PCR clones. The control region of the silkworm mt genome was located between the small ribosomal RNA gene and transfer RN $A^{Met}$. This 499 bp control region hale 95.4% A+T content. Extensive comparative analysis studies performed with similar control region of other insect genomes could not reveal a highly conserved region containing conserved motifs of animal mito-chondrial genome. The remarkable feature that found in this control region was the presence of tandem motifs containing nine repetitive sequences. The potential usefulness of this motif sequences for Bombyx species or their taxonomically related species is enhanced by its unique localization in the maternally inheritance mitochondrial molecule.e.

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An Efficient Partial Matching System and Region-based Representation for 2D Images (2D 영상의 효과적인 부분 정합 시스템과 영역기반 영상 표현)

  • Kim, Seon-Jong
    • Journal of Institute of Control, Robotics and Systems
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    • v.13 no.9
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    • pp.868-874
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    • 2007
  • This paper proposes an efficient partial matching system and representation by using a region-based method for 2D image, and we applied to an extraction of the ROI(Region of Interest) according to its matching score. The matching templates consist of the global pattern and the local one. The global pattern can make it by using region-based relation between center region and its rest regions in an object. And, the local pattern can be obtained appling to the same method as global, except relation between objects. As the templates can be normalized, we use this templates for extraction of ROI with invariant to size and position. And, our system operates only one try to match, due to normalizing of region size. To use our system for searching and examining if it's the ROI by evaluating the matching function, at first, we are searching to find candidate regions with the global template. Then, we try to find the ROI among the candidates, and it works this time by using the local template. We experimented to the binary and the color image respectively, they showed that the proposed system can be used efficiently for representing of the template and the useful applications, such as partially retrievals of 2D image.

Biogeography and Distribution Pattern of a Korean Wood-eating Cockroach Species, Cryptocercus kyebangensis, Based on Genetic Network Analysis and DNA Sequence Information

  • Park, Yung-Chul;Choe, Jae-Chun
    • Journal of Ecology and Environment
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    • v.30 no.4
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    • pp.331-340
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    • 2007
  • We examined the evolutionary and ecological processes shaping current geographical distributions of a Korean wood-eating cockroach species, Cryptocercus kyebangensis. Our research aims were to understand evolutionary pattern of DNA sequences, to construct genetic network of Cryptocercus kyebangensis local populations and to understand evolutionary and ecological processes shaping their current geographical distribution patterns via DNA sequence information and genetic networks, using sequence data of two genes (ITS-2 and AT region) from local populations of C. kyebangensis. The results suggest that the ITS-2 and AT region are appropriate molecular markers for elucidating C. kyebangensis geographic patterns at the population level. The MSN-A based on the ITS-2 showed two possible routes, the Hwaak-san and Myeongji-san route and the Seorak-san and Gyebang-san route, for migration of ancestral C. kyebangensis into South Korea. The MSNs (MSN-A and -B) elucidate migration routes well within South Korea, especially the route of Group I and Group II.

The Main Negative Factors of Military Influence on the Economic Environment of the Region and its Financial and Economic Security

  • Sytnyk, Yosyf;Havrychenko, Dmytro;Staverska, Tetiana;Primush, Roman;Erfan, Vitalii
    • International Journal of Computer Science & Network Security
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    • v.22 no.6
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    • pp.241-245
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    • 2022
  • The main purpose of the study is to determine the main factors influencing the economic environment of the region and its financial and economic security. Ukraine aspires to become a full member of the European Union, but the events of 2022 have changed everything. Full-scale military operations on the territory of Ukraine significantly affect its socio-economic situation Today, the issue of studying the negative impact of military operations on the economic environment is very relevant. Based on the results of the study, we have identified the main negative factors of the military impact on the economic environment of the region and its financial and economic security.

Phylogenetic Relationships Among Pleurotus species Inferred from Sequence Data of PCR Amplified ITS II Region in Ribosomal DNA (rDNA의 ITS II 부위의 염기서열분석에 의한 느타리버섯 종간의 근연관계)

  • Bae, Shin-Churl;Seong, Ki-Young;Lee, Shin-Woo;Go, Seung-Joo;Eun, Moo-Young;Rhee, In-Koo
    • The Korean Journal of Mycology
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    • v.24 no.2 s.77
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    • pp.155-165
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    • 1996
  • This study was carried out to identify the phylogenetic relationship among several isolates of Pleurotus species by comparing ITS II region of ribosomal DNA(rDNA) repeat unit. Two primers from ribosomal DNA sequences were chosen to amplify the specific internal transcribed spacer (ITS) II region of Pleurotus spp. The exact ITS II region with an unique band from six species of Pleurotus genus could be amplified using the two primers taken from at the 3'-end of 5.8S rDNA and 5'-end of 28S rDNA. Six representative species of the Pleurotus genus were easily characterized according to the length differences of ITS II region. Furthermore, within P. ostreatus species, different sizes of ITS II region could be observed in the isolates of ASI 2025 and ASI 2095 although they were classified as P. ostreatus by the conventional observation. The nucleotide sequence analyses of PCR-amplified ITS II region indicated that the isolates ASI 2025 and ASI 2095 were different from other Pleurotus spp. When the nucleotide sequences of six Pleurotus species were compared, three typical ITS II regions were highly variable especially at both ends of this region. The phylogenetic tree obtained by the Neighbor program of Felsenstein PHYLIP package with all the nucleotide sequence of Pleurotus spp. indicated that P. ostreatus, P. florida, P. sajor-caju and P. eryngii were closely related to one phylogenetic branch and P. cystidious was related to other branch with P. cornucopiae. The isolates ASI 2025 and 2038, however, were not closely related to any other Pleurotus spp. and formed their own individual branches.

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