• 한국균학회지
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• 제37권1호
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• pp.86-90
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• 2009

KACC에서 분양받은 C. militaris 4균주와 야생에서 채집된 6균주 그리고 교배 육성한 4균주를 대상으로 DNA분석에 의한 분류동정을 시행하였다. 표준균주 10균주와 교잡균주 4균주에서 조사된 전 부위(ITS1, 5.8S rDNA, ITS2)에서 많은 염기서열 차이를 보이고 있으나 같은 종으로 확인된 표준균주들과 수집균주들과 비교한 바 같은 균으로 분류된 것을 확인할 수 있었다. 교잡에 쓰여 졌던 표준 균주에서 교잡된 교잡균주 중 가장 우수한 교잡균주 mat1과 교잡에 사용되었던 표준균주(MPNU10306, MPNU10366)를 선택하여 RAPD 분석을 하였으며, RAPD에서 나타난 각 균주간의 다양한 유전적 변이가 관찰되었다. 이는 새로운 C. militaris종 균주로 판단되어지며, HPLC 분석에서도 교잡에 쓰여 졌던 표준균주보다 교잡한 C. militaris 균주가 cordycepin 생산량이 우수하게 나타났으며, 이는 교잡균주가 cordycepin 함량을 보다 더 높일 수 있음을 의미하고 상업적 가치가 있을 것이라 판단되어지며, 지속적인 연구가 필요할 것으로 생각된다.

• Fisheries and Aquatic Sciences
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• 제13권4호
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• pp.263-271
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• 2010

G protein-coupled receptors (GPCR) constitute the largest superfamily of cell membrane receptors, mediating diverse signal-transduction pathways. The melanocortin 4 receptor (MC4R) has been of interest for its physiological role and size, one of the smallest among the GPCRs, which makes it a good model system for the structural study of GPCRs. To study the molecular structure and tissue-specific expression of MC4R in olive flounder (Paralichthys olivaceus), the full-length MC4R gene was obtained using PCR amplification of genomic DNA as well as cDNA synthesis. Sequence analysis of the gene indicates that 978 bp of the MC4R gene encodes 325 amino acids without introns. Sequence alignment with the MC4Rs from other fish shows the highest degree of identity (96%) between Paralichthys olivaceous and Verasper moseri, followed by Takifugu rubripes and Tetraodon nigroviridis (89%). RNA was isolated from various tissues to examine the tissue distribution of MC4R by using RT-PCR. The results showed major expression of MC4R in the liver, brain, and eye, which is consistent with the expression pattern in other fish belonging to the order Pleuronectiformes.

• Parasites, Hosts and Diseases
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• 제55권3호
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• pp.279-285
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• 2017

The present study was performed to analyze molecularly the phylogenetic positions of human-infecting Trichostrongylus species in Mazandaran Province, Iran, which is an endemic area for trichostrongyliasis. DNA from 7 Trichostrongylus infected stool samples were extracted by using in-house (IH) method. PCR amplification of ITS2-rDNA region was performed, and products were sequenced. Phylogenetic analysis of the nucleotide sequence data was performed using MEGA 5.0 software. Six out of 7 isolates had high similarity with Trichostrongylus colubriformis, while the other one showed high homology with Trichostrongylus axei registered in GenBank reference sequences. Intra-specific variations within isolates of T. colubriformis and T. axei amounted to 0-1.8% and 0-0.6%, respectively. Trichostrongylus species obtained in the present study were in a cluster with the relevant reference sequences from previous studies. BLAST analysis indicated that there was 100% homology among all 6 ITS2 sequences of T. colubriformis in the present study and most previously registered sequences of T. colubriformis from human, sheep, and goat isolates from Iran and also human isolates from Laos, Thailand, and France. The ITS2 sequence of T. axei exhibited 99.4% homology with the human isolate of T. axei from Thailand, sheep isolates from New Zealand and Iran, and cattle isolate from USA.

• Animal Systematics, Evolution and Diversity
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• 제34권3호
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• pp.143-151
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• 2018

A marine ciliate Amphisiella milnei (Kahl, 1932) $Horv{\acute{a}}th$, 1950 was discovered from the tidal pool of Baekdo Island, South Korea. The existence of extra cirri between leftmost frontal cirrus and buccal cirrus discriminates this species from its congeners. Its morphological features are described as follows: body size in vivo $110-130{\times}35-45{\mu}m$; elongate rectangular to elliptical in shape; two large and several small ring-shaped structures; yellowish cortical granules arranged irregularly on ventral side but longitudinally along dorsal kineties on dorsal side; 34-40 adoral membranelles, 3 frontal cirri, 1 buccal cirrus, 1 parabuccal cirrus, usually 2 extra cirri behind leftmost frontal cirrus, and 3 frontoventral cirri; amphisiellid median cirral row composed of 25-31 cirri with 27-36 left and 27-44 right marginal cirri; usually 5 transverse cirri and 2 pretransverse cirri with 7 dorsal kineties; two macronuclear nodules. In addition to, 18S rDNA sequence of A. milnei was analyzed to understand its phylogenetic relationship.

• ALGAE
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• 제34권4호
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• pp.267-275
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• 2019

Three new species of the genus Micractinium were collected from five localities on the South Shetland Islands in maritime Antarctica, and their morphological and molecular characteristics were investigated. The vegetative cells are spherical to ellipsoidal and a single chloroplast is parietal with a pyrenoid. Because of their simple morphology, no conspicuous morphological characters of new species were recognized under light microscopy. However, molecular phylogenetic relationships were inferred from the concatenated small subunit rDNA, and internal transcribed spacer (ITS) sequence data indicated that the Antarctic microalgal strains are strongly allied to the well-supported genus Micractinium, including M. pusillum, the type species of the genus, and three other species in the genus. The secondary structure of ITS2 and compensatory base changes were used to identify and describe six Antarctic Micractinium strains. Based on their morphological and molecular characteristics, we characterized three new species of Micractinium: M. simplicissimum sp. nov., M. singularis sp. nov., and M. variabile sp. nov.

• 식물병연구
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• 제23권4호
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• pp.372-378
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• 2017

In this study, we identified the causative agent of post-harvest gray mold on broccoli that was stored on a farmers' cooperative in Pyeongchang, Gangwon Province, South Korea, in September 2016. The incidence of gray mold on broccoli was 10-30% after 3-5 weeks of storage at $3^{\circ}C$. Symptoms included brownish curd and gray-to-dark mycelia with abundant conidia on the infected broccoli curds. The fungus was isolated from infected fruit and cultured on potato dextrose agar. To identify the fungus, we examined the morphological characteristics and sequenced the rDNA of the fungus and confirmed its pathogenicity according to Koch's postulates. The results of the morphological examination, pathogenicity test, and sequencing of the 5.8S rDNA of the internal transcribed spacer regions (ITS1 and ITS4) and three nuclear protein-coding genes, G3PDH, HSP60, and RPB2, revealed that the causal agent of the post-harvest gray mold on broccoli was Botrytis cinerea. To our knowledge, this is the first report of post-harvest gray mold on broccoli in Korea.

• ALGAE
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• 제22권3호
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• pp.221-228
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• 2007

Frequent occurrences of green tides caused by Ulva species (Ulvales, Ulvophyceae) associated with eutrophication along enclosed coasts are currently causing environmental problems in coastal ecosystems. In addition, increasing intercontinental introductions of coastal marine organisms, including Ulva, are also a serious issue. However, due to the considerable morphological plasticity of this genus, the taxonomy of Ulva species based on morphological studies is problematic. Therefore, in order to elucidate the species diversity and seasonal changes of the dominant Ulva species in Mikawa Bay, central Honshu, Japan, we made seasonal collections of Ulva species at seven localities, and identified the dominant species using the ITS2 rDNA region sequences. We identified the following nine taxa as common Ulva species in the area: 1) Ulva pertusa Kjellman; 2) U. ohnoi Hiraoka et Shimada; 3) U. linza L.; 4) U. californica Wille; 5) U. flexuosa Wulfen; 6) U. fasciata Delile; 7) U. compressa L.; 8) U. armoricana Dion et al.; 9) U. scandinavica Bliding. Among the species, U. pertusa was most common and dominant from spring to summer, and U. ohnoi from autumn to winter. Ulva californica and U. scandinavica have not been reported before from Japan.

• 한국균학회지
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• 제43권4호
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• pp.277-280
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• 2015

In August 2015, we collected samples of gray mold from sweet basil growing in Sachunmeon, Gangneung, Gangwon Province, Korea. Symptoms included extensive growth of mycelia with gray conidia on young leaves, stems, and blossoms. The pathogen was isolated from infected leaves and blossoms and the fungus was cultured on potato dextrose agar. For identification of the fungus, morphology and rDNA sequencing analysis of the fungus were performed, which confirmed its pathogenicity according to Koch's postulates. The results of morphological examinations, pathogenicity tests, and the rDNA sequences of the internal transcribed spacer regions (ITS1 and ITS4) and the three nuclear protein-coding genes G3PDH, HSP60, and RPB2 showed that the causal agent was Botrytis cinerea. This is the first report of gray mold caused by Botrytis cinerea on sweet basil in Korea.

• 한국균학회지
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• 제37권1호
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• pp.108-110
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• 2009

영월과 진천의 2지역에서 잣나무로부터 내생균의 다양성을 조사하였다. 총 113개의 내생균을 분리하여 rDNA ITS 부위의 염기서열 분석으로 균을 동정하였다. 염기서열 분석결과 Pestalotiopsis sp.가 2지역에서 가장 높은 빈도로 분리되었다. 영월에서는 Pestalotiopsis sp. 68.5%, Lambertella sp. 12.4%로 분리되었으며, 진천에서는 Pestalotiopsis sp. 41.7%, Hypoxylon sp. 20.8%, 그리고 Phomopsis sp.가 12.4% 분리되었다. Hypoxylon sp.와 Phomopsis sp.는 영월에서 분리되지 않았다.

• ALGAE
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• 제36권3호
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• pp.175-193
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• 2021

This study provides the first report of the presence of Coolia malayensis in the Mediterranean Sea, co-occurring with C. monotis. Isolated strains from the Gulf of Gabès, Tunisia (South-eastern Mediterranean) were identified by morphological characterization and phylogenetic analysis. Examination by light and scanning electron microscopy revealed no significant morphological differences between the Tunisian isolates and other geographically distant strains of C. monotis and C. malayensis. Phylogenetic trees based on ITS1-5.8S-ITS2 and D1-D3/28S rDNA sequences showed that C. monotis strains clustered with others from the Mediterranean and Atlantic whereas the C. malayensis isolate branched with isolates from the Pacific and the Atlantic, therefore revealing no geographical trend among C. monotis and C. malayensis populations. Ultrastructural analyses by transmission electron microscopy revealed the presence of numerous vesicles containing spirally coiled fibers in both C. malayensis and C. monotis cells, which we speculate to be involved in mucus production.