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http://dx.doi.org/10.4489/KJM.2009.37.1.086

RAPD Analysis and Cordycepin Concentration of Hybrided Cordyceps militaris Strains by Mating  

Jeong, Jin-Woo (Department of Microbiology, College of Natural Sciences, Pusan National University)
Jin, Cheng-Yun (Department of Biochemistry, Dongeui University College of Oriental Medicine and Department of Biomaterial Control (BK21 program), Dongeui University Graduate School)
Kim, Mun-Ock (Department of Microbiology, College of Natural Sciences, Pusan National University)
Lee, Jae-Yun (Department of Microbiology, College of Natural Sciences, Pusan National University)
Choi, Yung-Hyun (Department of Biochemistry, Dongeui University College of Oriental Medicine and Department of Biomaterial Control (BK21 program), Dongeui University Graduate School)
Lee, Jae-Dong (Department of Microbiology, College of Natural Sciences, Pusan National University)
Publication Information
The Korean Journal of Mycology / v.37, no.1, 2009 , pp. 86-90 More about this Journal
Abstract
The C. militaris belongs to entomopathogenic fungi, which have their specific sequences in internal transcribed spacer regions (ITS1 and ITS2) depending on species. In this study, to identify the phylogenetic relationship of the strain hybrided by mating of C. militaris, we compared DNA sequences of ITS regions and 5.8S ribosomal DNA (rDNA) repeat unit of hybrid strain and its parental strains. The result revealed that hybrid strains are C. militaris species. In addition, cordycepins produced by hybrid strains and other strains of C. militaris were analyzed by HPLC with 20mM $KH_2PO_4$ of mobile phase and C-18 columns. The result indicated that the strain hybrided by mating produce higher concentration of phytochemical cordycepin than other C. militaris strains.
Keywords
Cordycepin; Cordyceps militaris; HPLC analysis; RAPD;
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