• 농업생명과학연구
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• 제45권6호
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• pp.1-7
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• 2011

This work was performed to confirm the molecular discrimination through the nrITS1 sequences among 3 taxa of Scirpus L. sensu lato (s.l.) species. S. planiculmis represented only 2 base sequence variations with S. maritimus in spite that they showed different morphological features. The nucleotide sequences of the ITS1 region from S. planiculmis were shown to have 99.1% homology with S. maritimus and 60.4% homology with S. triqueter. Although the morphology of S. planiculmis is similar with S. triqueter, molecular basis of the size and sequences on ITS1 region were shown to have distinctive differences. For divergency investigation on same sites and metapopulation, sequencing was conducted on ITS1 region with partial 5.8S and 18S regions. All plants of each species collected at the same site had identical band size pattern and sequences. Intraspecific molecular divergency was not identified in spite that these species live in different wetland sites. The ITS1 sequences described here provided a powerful genetic tool for phylogenetic studies which was difficult by morphological identification as high rate of morphological plasticity.

• 호남수학학술지
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• 제42권1호
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• pp.161-173
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• 2020

We introduce statistically localized sequences in 2-normed spaces and give some main properties of statistically localized sequences. Also, we prove that a sequence is statistically Cauchy sequence if and only if its statistical barrier is equal to zero. Moreover, we define the uniformly statistically localized sequences on 2-normed spaces and investigate its relationship with statistically Cauchy sequences.

• Animal cells and systems
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• 제5권4호
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• pp.283-290
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• 2001

Phylogenetic relationships among the Korean taxa of the genus Allium subgenus Rhizirideum and some related taxa were assessed on the basis of in sequences of nuclear ribosomal DNA. Twenty-eight accessions of the genus Allium L. consisting of subgenera Rhizirideum (19 taxa), Allium (5 taxa) and Amerallium (one taxon) were analyzed. The variation in the ITS region was informative at the levels of section except for sect. Reticulato- bulbosa which is known to be of multiple origin. The ITS 2 region was longer than the ITS 1 region, and all of the investigated Allium taxa were the same in length in the 5.8S region except for A. monanthum. Allium cyaneum var. cyaneum was the shortest (635 bp) and A. victorialis the longest (646 bp) among the investigated Korean taxa. The three morphologically similar taxa, A. thunbergii, A. sacculiferum that has been included in A. thunbergii, and A. deltoid- fistulosum, had the same ITS lengths of 641 bp, but were clearly distinguished in the phylogenetic analysis of their ITS sequences.

• 생명과학회지
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• 제13권4호
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• pp.400-411
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• 2003

5.8S rDNA를 포함한 ITS부위에 대한 염기서열 분석결과, 종에 따라 다양한 염기서열을 가지고 있어 분류에 이용될 수 있었으며, 특히 ITS2부위보다 ITS1부위에서 종에 대한 변이율이 높은 것으로 나타났다. 아울러 균종에 따라 정도의 차이는 있으나 사용된 모든 종들이 서로 계통분류학적 거리가 멀어서 종간의 구분이 명확하게 나타났다. P. tenuipes, I. japonica, P. japonicus는 multiple alignment분석에서 매우 유사한 염기서열을 가지고 있어, 이들 세종은 같은 종이지만 다른 이름으로 불리고 있는 것으로 나타났으며, 아울러 Paecilomyces sp. KACC 40220과 KACC 40656도 동일한 염기서열을 가지고 있어 p. tenuipes로 판단된다. 국내에서 유통되는 동충하초제품 35건과 중국산 1건에 대해 실험한 결과 23건은 P. tenuipes / japonica로, 11건은 C. militaris로, 1건은 B. bassiana로 분류되었으며, 중국산 제품 1건은 C. multiaxialis로 분류되었다.

• Mycobiology
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• 제28권1호
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• pp.11-16
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• 2000

The nucleotide sequences of the internal transcribed spacer (ITS) regions of the ribosomal DNA including the 5.8S ribosomal RNA gene (rDNA) have been determined for 11 species in order to analyze their intrageneric relationships. The total length of these sequences ranged from 530 nucleotides for Trichoderma reesei KCTC 1286 to 553 nucleotide for Trichoderma koningii IAM 12534. Generally speaking, the length of ITS1 region was about 30 nucleotides longer than that of the ITS2 region. Also, the sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites were also found. Thus, a neighbor-joining tree was constructed using the full sequence data of the ITS regions and the 5.8S rDNA. The Trichoderma genus used to be grouped on the basis of the morphological features and especially the shape of phialides needs to be reexamined. The phylogenetic tree displayed the presence of monophylogeny in the species of Trichoderma. Therefore, it was difficult to distinguish the intrageneric relationships in the Trichoderma genus.

• ALGAE
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• 제17권4호
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• pp.235-247
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• 2002

• Mycobiology
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• 제29권3호
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• pp.121-131
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• 2001

This study was carried out to identify the phylogenetic relationships among several caterpillar fungi by comparing the sequences of internal transcribed spacer regions(ITS1 and ITS2) and 5.8S ribosomal DNA(rDNA) repeat unit. The sequences of ITS1, ITS2, and the 5.8S rDNA from 10 strains of Cordyceps species, 12 strains of Paecilomyces, 3 strains of Beauveria, 2 strains of Metarhizium and 1 strains of Hirsutella were amplified, determined and compared with the previously known Cordyceps species. The sequences of 5.8S rDNA were more conserved in length and variation than those of ITS regions. Although the variable ITS sequences were often ambiguously aligned, the conserved sites could be found. In the phylogenetic tree, the species generally divided into three clusters, supported by their morphology and/or host ranges. The 5.8S rDNA and TTS1 sequences among 10 species of Cordyceps militaris were identical and only one base pair in ITS2 sequence was different. Cordyceps sinensis and Cordyceps ophioglossoides were also clearly different, although they belonged to the same cluster. The Geniank database search of species revealed sister taxa of an entomogenous fungus. Metarhizium was used as an putgroup in all taxa.

• 한국약용작물학회지
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• 제12권1호
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• pp.60-66
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• 2004

한국산 옻나무속 6종에 대하여 분자식물학적 방법으로 계통유연관계를 확인하기 위하여, nrDNA의 ITS 구간과 cpDNA rbcL 염기서열을 사용하여 계통분석한 결과 ITS 1의 길이는 $246{\sim}253\;bp$이었고, ITS 2는 $234{\sim}244\;bp$이었다. ITS 1의 길이는 Rhus sylvestris와 R. succedanea에서 246 bp로 가장 작았으며, R. verniciflua에서 253 bp로 가장 긴 것으로 나타났다. ITS 2의 길이는 R. verniciflua가 234 bp로 가장 짧았으며, R. trichocarpr가 244 bp로 가장 길게 나타났다. 이들 분류군의 G+C Content는 ITS 1에서는 $58.0{\sim}68.13%$의 범위를 나타냈고, ITS 2에서는 $59.75{\sim}68.46%$로 나타나 두 구간이 비슷한 비율을 보이고 있었다. ITS 1에서의 G+C content는 R. sylvestris가 58.0%로 가장 낮았으며, 가장 높은 값은 R. ambigua가 68.13%로 확인되었다. ITS 2에서는 외군인 Cotinus coggygria가 59.75%로 가장 낮았으며, R. ambigua가 68.46%로 가장 높게 나타났다. 한국산 옻나무 속에서 ITS 염기서열은 일반적으로 피자식물이 갖는 G+C content 범위 안에 포함되는 것으로 확인되었다. 한편, rbcL의 길이는 1,428 bp로 모든 종에서 동일하였다. 또한 rbcL의 G+C content는 $43.56%{\sim}43.77%$로 나타나 종간에 거의 차이가 없음을 확인하였다. 연구결과 rbcL gene은 옻나무속의 종간 계통유연관계를 해석하는데 유용하지 않았으며, ITS 1 구간의 염기서열 변이는 향후 옻나무속을 분류할 때 신속하게 분류할 수 있는 분류 marker로 이용할 수 있다고 판단되었다.

• Journal of Plant Biology
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• 제39권1호
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• pp.1-8
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• 1996

Sequences from the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA were determined to assess their potential as a phylogenetic tool for Korean Acer species, including A. okamotoannum and A. takesimense which are endemic on Ullung Island of volcanic origin. Although the genus Acer has been studied by various authors, different infrageneric dispositions have been suggested, and the phylogeny of the genus has been in dispute. The variation of ITS sequences from seven species of Acer was very low among species within the same section, but comparative analysesof the molecular data obtained suggest that ITS sequences may provide enough phylogenetic resolution for sectional relationships in the genus.

• 한국균학회지
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• 제27권2호통권89호
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• pp.124-131
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• 1999

본 실험은 진흙버섯류 7종 15균주에 대한 5.85 rDNA와 ITS 부위의 염기서열을 비교 분석함으로서 종간 및 종내의 유연관계를 조사하였다. 5.8S rDNA와 ITS 부위를 증폭하고자 18S rDNA의 3'말단 부위와 28S rDNA의 5'말단 부위에 두 개의 primer를 이용하여 PCR증폭을 행하였다. 5.8S rDNA와 ITS 부위를 증폭하여 염기서열을 비교 분석한 결과 본 실험에 공시된 Phellinus속의 제균종은 크게 4개의 cluster를 형성하였다. 첫 번째 cluster는 Phellinus hartigii IMSNU 32041, Phellinus robustus IMSNU 32068로 이루어졌고, 두 번째 cluster는 Phellinus linteus KCTC 6190, IMSNU 31014, DGUM 25003, DGUM 25004, Phellinus sp. DGUM 25007, Namsan No. 1과 Phellinus weirianus IMSNU 32021, 세 번째 cluster는 Phellinus laevigatus KCTC 6229, KCTC 6230과 Phellinus igniarius KCTC 6227, KCTC 6228로 이루어졌으며, Phellinus chrysoloma KCTC 6225와 KCTC 6226이 마지막 cluster를 형성하였다. 결과적으로 ITS 염기서열의 결과만으로 볼 때 Phellinus linteus와 Phellinus weirianus는 명확하게 종 단위의 개념을 정립할 수 없었다. 따라서 정확한 분류를 위해 생리학적, 분자생물학적 인 분류방법이 첨가되어야 하며, type strain에 대한 ITS 염기서얼도 결정되어야 한다. Phellinus속의 균들에서는 ITS2부위에 비해 ITS1부위의 변이율이 높았다. ITS 염기서열은 종 구분에 유용한 도구이며, 다른 균종들과 비교해 보았을 때 Phellinus linteus와 Phellinus weirianus에서만 ITS1 부위에서 특이적인 염기서열을 가지고 있었다.