Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
권호 표지

A Phylogenetic Study of Scirpus planiculmis F. Schm. (Cyperaceae) Based on ITS1 Sequences of Nuclear Ribosomal DNA

  • Jang, Wol-Suk (Department of Biology and Biomedical Science, Dong-A University) ;
  • Kang, Hye-Sook (Department of Biology and Biomedical Science, Dong-A University) ;
  • Han, In-Seop (School of Biological Science and Technology, College of Natural Science, Ulsan University) ;
  • Lee, Sun-Hee (Department of Biology and Biomedical Science, Dong-A University)
  • Received : 2011.05.18
  • Accepted : 2011.12.20
  • Published : 2011.12.30
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Abstract

This work was performed to confirm the molecular discrimination through the nrITS1 sequences among 3 taxa of Scirpus L. sensu lato (s.l.) species. S. planiculmis represented only 2 base sequence variations with S. maritimus in spite that they showed different morphological features. The nucleotide sequences of the ITS1 region from S. planiculmis were shown to have 99.1% homology with S. maritimus and 60.4% homology with S. triqueter. Although the morphology of S. planiculmis is similar with S. triqueter, molecular basis of the size and sequences on ITS1 region were shown to have distinctive differences. For divergency investigation on same sites and metapopulation, sequencing was conducted on ITS1 region with partial 5.8S and 18S regions. All plants of each species collected at the same site had identical band size pattern and sequences. Intraspecific molecular divergency was not identified in spite that these species live in different wetland sites. The ITS1 sequences described here provided a powerful genetic tool for phylogenetic studies which was difficult by morphological identification as high rate of morphological plasticity.

Keywords

Acknowledgement

Supported by : Dong-A university

References

  1. Amat, J. A. 1995. Effects of wintering graylag geese Anser anser on their Scirpus food plants. Ecography 18: 155-163. https://doi.org/10.1111/j.1600-0587.1995.tb00336.x
  2. Gibson, T., D. Higgins and J. Thompson. 1994. Clustal X program. EMBL Hidelberg Germany.
  3. Groenendijck, A. A and H. S. Yoon. 1985. Evaluation of one year research on the foodplant biomass for waterfowl in the Nakdong estuary, republic of Korea. In: Nakdong estuary barrage and reclamation project. Delta Institute NEDECO, 56pp.
  4. Hipp, A. L. 2008. Phylogeny and patterns of convergence in Carex sect. Ovales (Cyperaceae): evidence from ITS and 5.8S sequences In: Naczi RFC, Ford BA (eds) Sedges: uses, diversity, and systematics of the Cyperaceae. Missouri Botanical Garden, St. Louis, pp 197-214.
  5. Hirahara, T., T. Katsuyama, and T. Hoshino. 2007. Suprageneric phylogeny of Japanese Cyperaceae based on DNA sequences from chloroplast ndhF and 5.8S nuclear ribosomal DNA. Acta Phytotaxon Geobot. 58: 57-68.
  6. Jung, J. D. and H. K. Choi. 2010. Systematic rearrangement of Korean Scirpus L. s.l. (Cyperaceae) as inferred from nuclear ITS and chloroplast rbcL sequences. J. Plant Biol. 53: 222-232. https://doi.org/10.1007/s12374-010-9109-8
  7. Kim, C. K. and H. K. Choi. 1999. Cloning and structural analyses of partial nuclear rDNA from Bulpleurum euphorbioides (Apiaceae). J. Plant Biol. 42: 133-138.
  8. Koichiro, T., J. Dudley, M. Nei and S. Kumar. 2007. MEGA 4: Molecular evolution genetics analysis (MEGA) software version 4.0. Biol. Evol. 24: 1596-1599. https://doi.org/10.1093/molbev/msm092
  9. Muasya, A. M., D. A. Simpson, M. W. Chase, and A. Culham. 1998. An assessment of suprageneric phylogeny in Cyperaceae using rbcL DNA sequences. Plant Syst Evol. 211: 257-271. https://doi.org/10.1007/BF00985363
  10. Muasya, A. M., D. A. Simpson, G. A. Verboom, P. Goetghebeur, R.F.C. Naczi, M. W. Chase, and E. Smets. 2009. Phylogeny of Cyperaceae based on DNA sequence data: current progress and future prospects. Bot Rev (Lancaster) 75: 2-21. https://doi.org/10.1007/s12229-008-9019-3
  11. Roalson, E. H., J. T. Columbus, and E. A. Friar. 2001. Phylogenetic relationships in Cariceae (Cyperaceae) based on ITS (nrDNA) and trnTL-F (cp DNA) region sequences : assessment of subgeneric and sectional relationships in Carex with emphasis on section Acrocystis. Syst Bot. 26: 318-341.
  12. Simpson, D. A. 2008. Frosted curls to tiger nuts: ethnobotany of Cyperaceae. In: Naczi RFC, Ford BA (eds) Sedges: uses, diversity, and systematics of the cyperaceae. Missouri Botanical Garden, St. Louis, pp 1-14.
  13. Simpson, D. A., A. M. Muasya, M. V. Alves, J. J. Bruhl, S. Dhooge , M. W. Chase, C. A. Furness, K. Gramkhar, P. Goetghebeur, T. R. Hodkinson, A. D. Marchant, A. A. Reznicek, R. Nieuwborg, E. H. Roalson, E. Smets, J. R. Starr, W. W. Thomas, K. L. Wilson, and Z. Xiufu. 2007. Phylogeny of Cyperaceae based on DNA sequence data-a new rbcL analysis. Aliso 23: 72-83.
  14. Strong, M. T. 1993. New combination in Schoenoplectus (Cyperaceae) Novon 3: 202-203. https://doi.org/10.2307/3391535
  15. Suh, Y. 1992. DNA and reconstruction of plant phylogeny. Kor. J. Plant Tax. 22: 121-140.
  16. Suh, Y., H. J. Cho, S. Kim, and C. W. Park. 1996. Comparative analysis of ITS sequence from Acer species (Aceraceae) in Korea. J. Plant Biol. 39: 1-8.
  17. Suh Y, S. Kim, and C. W. Park. 1997. A phylogenetic study of Polygonum sect. Tovara (Polygonaceae) based on ITS sequences of nuclear ribosomal DNA. J. Plant Biol. 40: 47-53. https://doi.org/10.1007/BF03030320
  18. Yano, O., and T. Hoshino. 2005. Molecular phylogeny and chromosomal evolution of Japanese Schoenoplectus (Cyperaceae), based on ITS and ETS 1f sequences. Acta Phytotaxon Geobot. 56: 183-195.
  19. Yoon, H. S. 1987. Estimation of foodplant biomass and their use by waterfowl in the Nakdong estuary. Ph.D. Thesis, Kyung hee University, Seoul.