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http://dx.doi.org/10.5352/JLS.2003.13.4.400

Phylogenetic Analysis of the Entomopathogenic Fungal Species and Taxonomical Positions of Their Commercial Products  

김순한 (부산지방식품의약품안전청)
이영자 (부산지방식품의약품안전청)
김인복 (부산지방식품의약품안전청)
김미경 (부산지방식품의약품안전청)
한정아 (부산지방식품의약품안전청)
홍무기 (식품의약품안전청 식품평가부)
이순호 (식품의약품안전청 식품평가부)
이재동 (부산대학교 생명과학부)
Publication Information
Journal of Life Science / v.13, no.4, 2003 , pp. 400-411 More about this Journal
Abstract
This study was carried out to identify the phylogenetic relationship and to know the distribution of the entomopathogenic fungi by comparing the DNA sequences of internal transcribed spacer regions (ITS1 and ITS2) and 5.8S ribosomal DNA (rDNA) repeat unit. The entomopathogenic fungi had their specific sequences in ITS1 and 2 regions depending on species. The comparison of the ITS sequences of standard strains indicated that the sequences ITS1 were more variable than those of ITS2. It seems that Paecilomyces tenuipes, Isaria japonicus and P. japonicus are the same species but called as different names because of very similar sequences, and unidentified Paecilomyces sp. KACC 40220 and KACC 40656 showed identical sequences to P. tenuipes. Thirty six strains of the commercial products of entomopathogenic fungi used in this study were divided into four groups by the phylogenetic analysis based on 5.85 rDNA and ITS regions. We found twenty-three strains were P. tenuipes / japonica, eleven strains were C. militaris, and other two strains were Beauveria bassiana and C. multiaxialis, respectively.
Keywords
ITS; phylogenetic analysis; entomopathogenic fungi; Cordyceps; Paecilomyces;
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