• Title/Summary/Keyword: ITS II

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Molecular Analysis of Exophiala Species Using Molecular Markers

  • Chee, Hee-Youn;Kim, Yoon-Kyoung
    • Mycobiology
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    • v.30 no.1
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    • pp.1-4
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    • 2002
  • Genetic relatedness of medically important Exophiala species such as E. dermatitidis, E. mansonii, and three E. jeanselmei varieties: jeanselmei, lecanii-corni, and heteromorpha was examined using PCR-RFLP(restriction fragment length polymorphism) of ribosomal DNA, M-13, $(GTG)_5$ and nucleotide sequences of ribosomal ITS(internal transcribed space) II regions. Three E. jeanselmei varieties showing distinct band patterns for each DNA markers as well as different nucleotide sequences of ribosomal ITS II regions could be considered as a separate species. E. dermatitidis and E. mansonii demonstrated the identical band patterns of RFLP of ribosomal DNA, M-13, and $(GTG)_5$ markers. However, nucleotides sequences of ribosomal ITS II region were different between these two species.

Localization and Autonomous Control of PETASUS System II for Manipulation in Structured Environment (구조화된 수중 환경에서 작업을 위한 PETASUS 시스템 II의 위치 인식 및 자율 제어)

  • Han, Jonghui;Ok, Jinsung;Chung, Wan Kyun
    • The Journal of Korea Robotics Society
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    • v.8 no.1
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    • pp.37-42
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    • 2013
  • In this paper, a localization algorithm and an autonomous controller for PETASUS system II which is an underwater vehicle-manipulator system, are proposed. To estimate its position and to identify manipulation targets in a structured environment, a multi-rate extended Kalman filter is developed, where map information and data from inertial sensors, sonar sensors, and vision sensors are used. In addition, a three layered control structure is proposed as a controller for autonomy. By this controller, PETASUS system II is able to generate waypoints and make decisions on its own behaviors. Experiment results are provided for verifying proposed algorithms.

Mapping of the Interaction Domain of DNA Topoisomerase $II{\alpha}$ and $II{\beta}$ with Extracellular Signal-Regulated Kinase 2

  • Park, Gye-Hwa;Bae, Young-Seuk
    • BMB Reports
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    • v.34 no.1
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    • pp.85-89
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    • 2001
  • Both topoisomerase $II{\alpha}$ and $II{\beta}$ east as phosphoproteins in the cells. Recently it was reported that DNA topoisomerase $II{\alpha}$ associates with and is phosphorylated by the extracellular signal-regulated kinase 2 (ERK2). Also, ERK2 stimulates the activity of topoisomerase II by a phosphorylation-independent manner [Shapiro et al., (1999) Mol. Cell. Biol. 19, 3551-3560]. In this study, a yeast two-hybrid system was used to investigate the binding site between topoisomerase $II{\alpha}$ or $II{\beta}$ and ERK2. The two-hybrid test clearly showed that topoisomerase $II{\beta}$ residues 1099-1263, and topoisomerase $II{\alpha}$ residues 1078-1182, mediate the interaction with ERK2, and that the leucine zipper motifs of topoisomerase $II{\alpha}$ and $II{\beta}$ are not required for its physical binding to ERK2. Our results suggest that topoisomerase $II{\beta}$ residues 1099-1263, and topoisomerase $II{\alpha}$ residues 1078-1182, may be common binding sites for activator proteins.

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Combined Economic and Emission Dispatch with Valve-point loading of Thermal Generators using Modified NSGA-II

  • Rajkumar, M.;Mahadevan, K.;Kannan, S.;Baskar, S.
    • Journal of Electrical Engineering and Technology
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    • v.8 no.3
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    • pp.490-498
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    • 2013
  • This paper discusses the application of evolutionary multi-objective optimization algorithms namely Non-dominated Sorting Genetic Algorithm-II (NSGA-II) and Modified NSGA-II (MNSGA-II) for solving the Combined Economic Emission Dispatch (CEED) problem with valve-point loading. The valve-point loading introduce ripples in the input-output characteristics of generating units and make the CEED problem as a non-smooth optimization problem. IEEE 57-bus and IEEE 118-bus systems are taken to validate its effectiveness of NSGA-II and MNSGA-II. To compare the Pareto-front obtained using NSGA-II and MNSGA-II, reference Pareto-front is generated using multiple runs of Real Coded Genetic Algorithm (RCGA) with weighted sum of objectives. Furthermore, three different performance metrics such as convergence, diversity and Inverted Generational Distance (IGD) are calculated for evaluating the closeness of obtained Pareto-fronts. Numerical results reveal that MNSGA-II algorithm performs better than NSGA-II algorithm to solve the CEED problem effectively.

Synthesis of Diketo Copper(II) Complex and Its Binding toward Calf Thymus DNA (CTDNA) (이케토 구리(II) 착물의 합성 및 송아지 Thymus DNA(CTDNA)와의 상호작용)

  • Tak, Aijaz Ahmad;Arjmand, Farukh
    • Journal of the Korean Chemical Society
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    • v.55 no.2
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    • pp.177-182
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    • 2011
  • A diketo-type ligand was synthesized by the Knoevenagel condensation reaction of thiophene-2-aldehyde with acetylacetone, subsequently its transition metal complexes with Cu(II), Ni(II), and Co(II) chlorides were also prepared. All the complexes were characterized by various physico-chemical methods. The molar conductivity data reveals ionic nature for the complexes. The electronic spectrum and the EPR values suggest square planar geometry for the Cu(II) ion. Interaction of the Cu(II) complex with CTDNA (calf thymus DNA) was studied by absorption spectral method and cyclic voltammetry. The $k_{obs}$ values versus [DNA] gave a linear plot suggesting psuedo-first order reaction kinetics. The cyclic voltammogram of the Cu(II) complex reveals a quasi-reversible wave attributed to Cu(II)/Cu(I) redox couple for one electron transfer with $E_{1/2}$ values -0.240 V and -0.194 V. respectively. On addition of CTDNA, there is a shift in the $E_{1/2}$ values 168 mV and 18 mV respectively and decrease in Ep values. The shift in $E_{1/2}$ values in the presence of CTDNA suggests strong binding of Cu(II) complex to the CTDNA.