• 제목/요약/키워드: ITS II

검색결과 3,545건 처리시간 0.032초

Molecular Analysis of Exophiala Species Using Molecular Markers

  • Chee, Hee-Youn;Kim, Yoon-Kyoung
    • Mycobiology
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    • 제30권1호
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    • pp.1-4
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    • 2002
  • Genetic relatedness of medically important Exophiala species such as E. dermatitidis, E. mansonii, and three E. jeanselmei varieties: jeanselmei, lecanii-corni, and heteromorpha was examined using PCR-RFLP(restriction fragment length polymorphism) of ribosomal DNA, M-13, $(GTG)_5$ and nucleotide sequences of ribosomal ITS(internal transcribed space) II regions. Three E. jeanselmei varieties showing distinct band patterns for each DNA markers as well as different nucleotide sequences of ribosomal ITS II regions could be considered as a separate species. E. dermatitidis and E. mansonii demonstrated the identical band patterns of RFLP of ribosomal DNA, M-13, and $(GTG)_5$ markers. However, nucleotides sequences of ribosomal ITS II region were different between these two species.

구조화된 수중 환경에서 작업을 위한 PETASUS 시스템 II의 위치 인식 및 자율 제어 (Localization and Autonomous Control of PETASUS System II for Manipulation in Structured Environment)

  • 한종희;옥진성;정완균
    • 로봇학회논문지
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    • 제8권1호
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    • pp.37-42
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    • 2013
  • In this paper, a localization algorithm and an autonomous controller for PETASUS system II which is an underwater vehicle-manipulator system, are proposed. To estimate its position and to identify manipulation targets in a structured environment, a multi-rate extended Kalman filter is developed, where map information and data from inertial sensors, sonar sensors, and vision sensors are used. In addition, a three layered control structure is proposed as a controller for autonomy. By this controller, PETASUS system II is able to generate waypoints and make decisions on its own behaviors. Experiment results are provided for verifying proposed algorithms.

Mapping of the Interaction Domain of DNA Topoisomerase $II{\alpha}$ and $II{\beta}$ with Extracellular Signal-Regulated Kinase 2

  • Park, Gye-Hwa;Bae, Young-Seuk
    • BMB Reports
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    • 제34권1호
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    • pp.85-89
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    • 2001
  • Both topoisomerase $II{\alpha}$ and $II{\beta}$ east as phosphoproteins in the cells. Recently it was reported that DNA topoisomerase $II{\alpha}$ associates with and is phosphorylated by the extracellular signal-regulated kinase 2 (ERK2). Also, ERK2 stimulates the activity of topoisomerase II by a phosphorylation-independent manner [Shapiro et al., (1999) Mol. Cell. Biol. 19, 3551-3560]. In this study, a yeast two-hybrid system was used to investigate the binding site between topoisomerase $II{\alpha}$ or $II{\beta}$ and ERK2. The two-hybrid test clearly showed that topoisomerase $II{\beta}$ residues 1099-1263, and topoisomerase $II{\alpha}$ residues 1078-1182, mediate the interaction with ERK2, and that the leucine zipper motifs of topoisomerase $II{\alpha}$ and $II{\beta}$ are not required for its physical binding to ERK2. Our results suggest that topoisomerase $II{\beta}$ residues 1099-1263, and topoisomerase $II{\alpha}$ residues 1078-1182, may be common binding sites for activator proteins.

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Combined Economic and Emission Dispatch with Valve-point loading of Thermal Generators using Modified NSGA-II

  • Rajkumar, M.;Mahadevan, K.;Kannan, S.;Baskar, S.
    • Journal of Electrical Engineering and Technology
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    • 제8권3호
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    • pp.490-498
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    • 2013
  • This paper discusses the application of evolutionary multi-objective optimization algorithms namely Non-dominated Sorting Genetic Algorithm-II (NSGA-II) and Modified NSGA-II (MNSGA-II) for solving the Combined Economic Emission Dispatch (CEED) problem with valve-point loading. The valve-point loading introduce ripples in the input-output characteristics of generating units and make the CEED problem as a non-smooth optimization problem. IEEE 57-bus and IEEE 118-bus systems are taken to validate its effectiveness of NSGA-II and MNSGA-II. To compare the Pareto-front obtained using NSGA-II and MNSGA-II, reference Pareto-front is generated using multiple runs of Real Coded Genetic Algorithm (RCGA) with weighted sum of objectives. Furthermore, three different performance metrics such as convergence, diversity and Inverted Generational Distance (IGD) are calculated for evaluating the closeness of obtained Pareto-fronts. Numerical results reveal that MNSGA-II algorithm performs better than NSGA-II algorithm to solve the CEED problem effectively.

이케토 구리(II) 착물의 합성 및 송아지 Thymus DNA(CTDNA)와의 상호작용 (Synthesis of Diketo Copper(II) Complex and Its Binding toward Calf Thymus DNA (CTDNA))

  • Tak, Aijaz Ahmad;Arjmand, Farukh
    • 대한화학회지
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    • 제55권2호
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    • pp.177-182
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    • 2011
  • 이케토형 리간드는 thiophene-2-aldehyde와 acetylacetone의 Knoevenagel 축합반응을 통해 합성하였으며, 이를 이용하여 Cu(II), Ni(II) 및Co(II) 염화물의 착물을 합성하였다. 모든 착물의 특성은 다양한 물리-화학적 방법으로 규명하였다. 몰전기전도도 결과로부터 이들 착물이 이온성을 가짐을 알았다. 전자 및 EPR 스펙트럼을 통해 구리(II) 이온이 사각평면 기하구조를 가짐을 알았다. 구리(II) 착물과 CTDNA(송아지 thymus DNA)의 상호작용을 흡수 스펙트럼과 순환 전압전류법으로 연구하였다. $k_{obs}$ 대 [DNA]의 도시는 선형을 보였는데, 이는 유사-1차반응을 의미한다. 순환 전압전류 그림으로부터 구리(II) 착물이 각각 -0.240 V와 -0.194 V의 $E_{1/2}$ 값을 갖는 일전자 Cu(II)/Cu(I) 산화-환원 쌍에 대해 준가역적임을 알았다. CTDNA를 첨가한 경우, $E_{1/2}$값이 각각168 mV와 18 mV 이동하였고 Ep 값도 감소하였다. CTDNA의 존재 하에 $E_{1/2}$이 이처럼 이동하는 것은 구리(II) 착물이 CTDNA에 강하게 결합됨을 의미한다.