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Optimized pretreatment conditions for the environmental DNA (eDNA) analysis of Apostichopus japonicus

  • Kang, Yu-An;Lee, Soo Rin;Kim, Eun-Bi;Park, Sang Un;Lim, Sang Min;Andriyono, Sapto;Kim, Hyun-Woo
    • Fisheries and Aquatic Sciences
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    • v.25 no.5
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    • pp.264-275
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    • 2022
  • A non-destructive environmental DNA protocol for the genetic analysis of sea cucumber (Apostichopus japonicus) resources DNA was established. Among the several commercial DNA extraction kits, the DNeasy® Plant Mini Kit was selected as the best choice to obtain the high-quality genomic DNAs from the mucous sea cucumber. As the temperature and incubation time increased, the amount of extracted environmental DNA was also large, but it was judged that the increased amount did not affect as much as 2-3 times. Therefore, these conditions were not considered to be the main factors to consider in actual environmental DNA extraction. However, the amount of seawater relative to the size of the sample was judged as a major consideration, and a sufficient amount of environmental DNA for analysis was secured when stored within 1 min while stirring the volume of seawater corresponding to the total sea cucumber weight (g). In securing the environmental DNA of sea cucumbers, the mortality rate of sea cucumbers in all experiments was 0, and it was judged that the effects of sea cucumbers were not significant through this treatment. Through the results of this study, sea cucumber DNA research, which has been conducted in a destructive method, can be conducted non-destructively through environmental DNA analysis. Through this study, we have secured a standard protocol that can successfully extract the sea cucumber DNA through environmental DNA. It is not only excellent in terms of time and cost of traditional DNA analysis method currently used, but it is completely non-destructive in the ecosystem of the survey area. It is believed that the system can be transformed in a way that does not affect it. However, it is thought that various standard protocols should be established considering the characteristics of each type.

Ecological Characteristics and Native Preservation Method of Glehnia littoralis Community in Korea Coast (갯방풍 자생지의 식생구조 및 군락특성에 관한 연구)

  • Choo, Byung Kil;Ji, Yunui;Moon, Byeong Cheol;Kim, Bobae;Lee, A-Yeong;Yoon, Taesook;Song, Hokyung;Kim, Ho Kyoung
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.11 no.6
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    • pp.38-48
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    • 2008
  • This study was conducted to evaluate the vegetation structure of community by the phytosociology method, floristic composition table on coast of South Korea. Form 2007 June until November, $2m{\times}2m$ quadrat was established in native Glehnia littoralis in order to record a dominants and coverage, and it drew the profile. It was found out that the mean temperature in the distributed areas for Glehnia littoralis population was $11^{\circ}C$ or more. The flora of the studied area in Glehnia lottoralis community of coastal dune was listed as 100 species. Glehnia lottoralis community of appearance species of Yeonggwanggun Duwori was many most by 44 species. Carex pumila, Carex Kobomugi, Imperata cylindrica var. koenigii, Ischaemum anthephehoroides and Vitex rotundifolia range all over the studied areas. And the vegetation of Glehnia littoralis community was classified into Vitex rotundifolia subcommunity, Ischaemun anthephephoroides subcommunity and Imperata cylindrica var. koenigii subcommunity. Native Glehnia littoralis was classified into preserve area, natural selection area and artificial destruction area. It must preserve native Glehnia littoralis of Goseong, Yeongdeok, Haenam it was ecological important area.

Technological and Social Significance of the Revision of the Radio Law (전파법 개정에 따른 기술·사회적 중요성)

  • Yang, Jeong-Won;Seok, Gyeong-Hyu;Shin, Hyun-Shin
    • The Journal of the Korea institute of electronic communication sciences
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    • v.14 no.4
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    • pp.627-636
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    • 2019
  • The Radio Law was revised twelve times since the full revision in 2000, and now it is equipped with the current status of securing radio resources, distribution and allocation of radio resources, utilization of radio resources, protection of radio resources and promotion of radio waves, which can be evaluated to include the legal nature of securing radio resources and propagation beyond the simple administrative legal nature of radio resources. The legal system in the telecommunication sector is also being improved, and the Radio Law is also supplementing the weak points through two revisions. The domestic radio law, and it is considered to form a legal system for promoting the effective allocation and utilization of resources in accordance with the changes in radio wave usage environment. It can be evaluated that it has become a law related to radio promotion and competition in the existing simple administrative law. It is considered necessary to adjust the detailed regulations for each type of use.

Defining a Smart Water City and Investigating Global Standards

  • Lee, Jung Hwan;Jang, Su Hyung;Lee, Yu Jin
    • Proceedings of the Korea Water Resources Association Conference
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    • 2022.05a
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    • pp.505-505
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    • 2022
  • This study shows the first output of the three-year project (2021-2023) to develop a Smart Water City (SWC) Global Standard and Certification Scheme ley by K-water, International Water Resources Association (IWRA) and Asia Water Council (AWC). There are three major parts in the first year. In Part 1, it investigates the essential features of cities today and details the water challenges currently faced and likely to be confronted in the future. It also investigates the functions that water fulfills in the urban environment, and how ICTs can contribute to improving those functions by each Urban Water Cycle. A definition of a Smart Water City is proposed following a discussion on the meaning of "smart development". This part of the report also presents different city cases from countries around the world to illustrate the urban water challenges and the technological and non-technological solutions that cities have put in place, including national and/or local policies and strategies. In Part 2, it defines what global standards indicators and certification schemes are and identifies their characteristics. Especially, it analyses in detail eight relevant standards and certification schemes measuring sustainable development and/or water resources management in urban settings. Standards elaborated by international organizations are distinguished from those developed by the private sector, non-governmental organizations, and by academia. Finally, this study suggests the right direction to develop SWC global standard frameworks and certification schemes. And then, it shows the main tasks for the Stage 2 (second year) project. Basically, the framework for a future SWC standard (consisting three main pillars: Technical, Governance and Prospective pillars) will be fully defined in Stage 2.

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Development of a New Islanding Detection Method for Distributed Resources (분산 전원의 고립 운전 검출 기법의 개발)

  • Jang, Seong-Il;Kim, Gwang-Ho
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.50 no.11
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    • pp.506-513
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    • 2001
  • The islanding detection for distributed resources (DR) becomes an important and emerging issue in power system protection since the distributed generator installations are rapidly increasing and most of the installed systems are interconnected with distribution network. In order to avoid the negative impacts from islanding operations of DR on protection, operation and management of distribution system, it is necessary to effectively detect the islanding operations of DR and rapidly disconnect it from distribution network. Generally, it is difficult to detect islanding operation by monitoring only one system parameter This paper presents a new logic based islanding detection method for distributed resources(DR) which are interconnected with distribution network. The proposed method detects the islanding operation by monitoring four system parameter: voltage variation, phase displacement, frequency variation, and the variation of total harmonic distortion(THD) of current; therefore, it effectively detects island operation of DR unit operating in parallel with the distribution network. We also verified the efficiency of the proposed algorithm using the radial distribution network of IEEE 34 bus model.

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The Internet: An (other) agent that disseminates Japanese 'soft power' resources

  • Bunyavejchewin, Poowin
    • Journal of Contemporary Eastern Asia
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    • v.11 no.1
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    • pp.21-29
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    • 2012
  • The popularity of the Internet has affected international politics in many ways; however, it is seemingly overlooked by most scholars, and in particular, realists who view the Internet as low-politics. This article argues that the impact of the Internet on international politics should not be underestimated. By focusing on the capabilities of the Internet in general and P2P networks in particular, this paper shows how the Internet is able to disseminate soft power resources. This is demonstrated by an examination of the dissemination of Japanese soft power resources through BitTorrent. Finally, it is concluded that the Internet is a plausible agent for distributing the resources of soft power; however, once disseminated, soft power resources do not always transform into soft power. In the end, contextual conditions within states always apply.

Neotypification of Veronica pusanensis (Scrophulariaceae)

  • Jang, Hyun-Do;Noh, Tae-Kwon
    • Journal of Species Research
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    • v.9 no.4
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    • pp.375-376
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    • 2020
  • A type specimen is the specimen originally used to name a new species; thus, it is one of the most significant materials for taxonomic study. In our study on the inventory and management of endemic species in Korea, we checked the type materials for Pseudolysimachion pusanensis (Y. Lee) Y. Lee, combination name Veronica pusanensis Y. Lee, recorded as deposited at the herbaria of the Ewha Womans University and the National Institute of Biological Resources, and failed to find any specimens. Thus, we concluded that all type specimens of V. pusanensis have since been misplaced. According to articles 9.11 and 9.13 of the International Code of Nomenclature for algae, fungi, and plants, it is necessary to select a neotype if the holotype is missing and no other original material exists. Therefore, we designate the neotype maintained in the herbarium of the National Institute of Biological Resources of the Republic of Korea.

The Effects of Job Demands and Job Resources on Job Burnout - A Comparison of office workers with service employees. (직무요구와 직무자원이 직무탈진에 미치는 영향 - 일반 사무직과 서비스직의 비교)

  • Yoon, Jang-Won
    • Journal of Applied Reliability
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    • v.6 no.4
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    • pp.255-274
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    • 2006
  • Recently firms become largely changed because of rapid technological innovation and serious global competition. It induces job stress of workers and finally leads to job burnout. This study aims to find the effects of job demands and job resources on job burnout. Job demands contains role ambiguity, role conflict, role overload and job characteristics. Job resources contains job control and feedback, involvement in decision making, learning opportunity and social support. The result reveals that job demands raises job burnout and job resources decreases job burnout. And it reveals that the effects of job demands and job resources on job burnout differs slightly according to job categories.

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Availability and Utilizations of Library Information Resources in Secondary Schools by Special Patrons

  • Asuata, Isibhakhome Eleaena;Emasealu, Helen Uzoezi
    • International Journal of Knowledge Content Development & Technology
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    • v.12 no.1
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    • pp.35-47
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    • 2022
  • This study adopted the descriptive survey research method. The population of this study comprised of the hearing impaired students in selected government secondary schools in Rivers state. The population of hearing impaired students in Rivers State is 257. A simple random sampling technique was used to select a sample size of 159. A self-developed instrument titled "Availability and Utilization of Library Information Resources Questionnaire (AULIRQ)" was utilized for data collection. 153 responses were collated, indicating a 96.2% response rate. The data collected was arranged and analyzed using frequency count, percentages, and mean and standard deviation. Finding reveals that information resources in the libraries understudied were inadequate and under-utilized. Also, it was established that among other barriers, under-funding is a major barrier to making information resources available for the physically challenged. It was recommended that schools offering special education for the hearing impaired should pace up in their developmental gap by providing such resources and ensure adequate funding by all stakeholders.